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The map label for this gene is purU [H]

Identifier: 33864517

GI number: 33864517

Start: 2384617

End: 2385456

Strand: Reverse

Name: purU [H]

Synonym: PMT2253

Alternate gene names: 33864517

Gene position: 2385456-2384617 (Counterclockwise)

Preceding gene: 33864519

Following gene: 33864516

Centisome position: 98.95

GC content: 52.02

Gene sequence:

>840_bases
GTGATTCTGCAGCTCATCTGTCCAGATCGGCCTGCTTTGGTGAGTGAGTTGTCGGGATGGGTGGCTGTTAATGGAGGCAA
TATTCTTCATGCTGATCACCATACGGATGTTGGGGCTGGACTGTTTCTGAGCAGGATCGAATTTGGAATTGAGGGCTTCG
GATTGCCTCGAGAGGCGATAGCACCGGCTGTGAATGCTCTGGCAGATCGCCTCGGGGGTCAGGCGCAATTGCATTTTTCG
GATGAGATCCCTCGGGTGGCGATCTTCGCCAGCAAGCAGAGTCACTGTCTGTTGGATCTGCTTTGGCGCACTCGCAGTGG
GGAGTTGCCGATGCAGGTGCCGCTTGTGATTGCCAACCACTCTCAATTGGAGCCTTTATGCAGGGAGTTTGGTGTTTGTT
TTGAGTGTGTTCCTATGACGCCTGCCAGCAAGCCTGAGGCGGAACAAACCATGCTGGATTTGTTGGCTGAGCATCGGATT
GAGCTGGTTGTGTTGGCCAAGTACATGCAGGTCCTTAGTGGTGCTTTTCTAGAGCGTTTCTCCACCGTGATTAATATTCA
CCATTCGTTCCTACCTGCTTTTAAGGGGGCACAGCCCTATCACAGAGCCTGGGATCGAGGGGTGAAAGTTATTGGTGCTA
CCGCCCATTACGTCACTGAAGATCTGGATGACGGGCCGATTATTGAGCAGACCATCGAGCATGTAAACCATCGTGATGAG
GTGGAAGATTTGATTCGTAAAGGACGTGATACGGAACGTCTCGCTTTGGCAAGGGCCTTGAGATTGCATCTTTGTCGCCA
GGTGATGGTCTATCGCGGTAGAACCGCTGTTTTTGCATGA

Upstream 100 bases:

>100_bases
TTGGTGCTGATGTTCGTCAAGACAGAATGAGTTTTTGCAATCAGGTTGAGTGAGCTCCCCTCTTGGTGCTGTATGGAATC
CTTACGGTTACAAGCTTCGT

Downstream 100 bases:

>100_bases
CGGTGTTGGCTTGGATGGCTCGATGTCGCCCTGCTGCTGCGACCTCCATGGGTTGGTTGGTTTTCCAGTTGGGGCTTTTT
TTGTTGCCTTCCAGTGCATT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAIAPAVNALADRLGGQAQLHFS
DEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPASKPEAEQTMLDLLAEHRI
ELVVLAKYMQVLSGAFLERFSTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLDDGPIIEQTIEHVNHRDE
VEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA

Sequences:

>Translated_279_residues
MILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAIAPAVNALADRLGGQAQLHFS
DEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPASKPEAEQTMLDLLAEHRI
ELVVLAKYMQVLSGAFLERFSTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLDDGPIIEQTIEHVNHRDE
VEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA
>Mature_279_residues
MILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAIAPAVNALADRLGGQAQLHFS
DEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPASKPEAEQTMLDLLAEHRI
ELVVLAKYMQVLSGAFLERFSTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLDDGPIIEQTIEHVNHRDE
VEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=40.6474820143885, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=35.5421686746988, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=28.7179487179487, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31100; Mature: 31100

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAI
CEEEEECCCCHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHH
APAVNALADRLGGQAQLHFSDEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANH
HHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEECC
SQLEPLCREFGVCFECVPMTPASKPEAEQTMLDLLAEHRIELVVLAKYMQVLSGAFLERF
HHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLDDGPIIEQTIEHVNHRDE
HHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECHHHHHHHCCCCCCHHHHHHHHCCCHHH
VEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAI
CEEEEECCCCHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHH
APAVNALADRLGGQAQLHFSDEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANH
HHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEECC
SQLEPLCREFGVCFECVPMTPASKPEAEQTMLDLLAEHRIELVVLAKYMQVLSGAFLERF
HHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLDDGPIIEQTIEHVNHRDE
HHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECHHHHHHHCCCCCCHHHHHHHHCCCHHH
VEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]