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The map label for this gene is gspD [H]

Identifier: 33864362

GI number: 33864362

Start: 2210432

End: 2212756

Strand: Direct

Name: gspD [H]

Synonym: PMT2098

Alternate gene names: 33864362

Gene position: 2210432-2212756 (Clockwise)

Preceding gene: 33864361

Following gene: 33864364

Centisome position: 91.69

GC content: 48.73

Gene sequence:

>2325_bases
ATGTCTCGTTCCCAGCGACTGGTTCCTTTGCTTGCAGCTCTTGGTTTGTATGGCGCGTCGATAAGCGGTGGCTTGATGCT
GCCGGCTGCAGTAAAGGCACAGAAGCAGGGGACGATGGAGCTGAAGTTGCGACGCTCGCAGGACAGCGTTGAAGTCATCA
TTGAAGGTGTGGGCCCACAGCCCGTTGTGCGGCAGCGACAGACTGGTCAGGACTGGGAAGGTCGATTGACTACCCAGGGC
AAACCTGGTCTGCGACGCGGTCCACAGCAAGTATCGATGCCGCAACTGGGGCTGCAATCTGTGAGCCTTGCAGGATCAGG
CGAGAGCTATCAATTAAATGTTGAAGGGGTGGCCGGCAGGCCGTTAATGGAACCATTGGTTAGTGCCGACGGCCGCAATT
TGATCCTTAGCTTTCCTGGGTTGACGGCTCAGGCTAAGCAAACTGGACGATTGGATCTACGCACACCAGGGCGGGTGCCT
CAGGCGTCTTATGTACCGCCTTTGCAACAACGTGCTGTAGCGCCTCCACTAGGAGATATGGCCGTGGGCACCATGGTGCT
GAGTAACCGCAGTTTTGTGCAGGTGAGTGGTCCTTCAGTGACTCTGACCTTGAACAATGCTCCTGCGAAAGATGCCTTGA
TGTCTCTTGCTCGATTGGGAGGCTATGGATTCATTTTTGTAGGGGATGATTCAGAAAGTGGAAACACGACTGGAGTGGGC
ACATCAGCAGATGCTCCAGTCAATGGTGGGAATAAGAAGGTGTCGATGGCGTTTCAAAATGAGAGCTATGCCCGTGCCCT
CAATGGGGTGCTGTTGGCATCAGGGTTACAGGGAAAGCTTGATGGCCGCACGTTGCTTGTTGGCAGTGCCGTTGCGAGTA
AAACATTTGGGCCTCAGGTTTCGAAGGTATATCGGTTGAATCAAGCAAGTGCTGCTTCTGCGGCTGACTACTTGGCCAGC
CTCGGAGCTGCGATCAGTAAGGTGAATATCGTCAGCATTACATCTGGCGAGGCATCATCCGCAGGGACATCTCAGCTCAG
CAATGAGGTTTCACAAACGTCTTCTAAGACGACGAGTATTGAAACCTACGGAGCATCAGTTGGCCCTCTGAGGGGTCTTA
CAGGTACAACTGATTCTCGCTTGCAAACAATCACATTGGTAGGGGATTCTCAATTGGTTTCTGTGGCGGAGAGCTATCTC
AAGCAGATCGATCTGCGCTCTAGGCAGGTGGCGTTATCAGTGAAGATTTTAGATGTTAATTTAAACAATAACAGCGAAAT
GGATAATAGCTTTTCGGTGCGGTTTGGAGATAATTTAATTGTTAATGATAATGGCGAATTACTAGCAGCATTCGGCAGTA
ATATGCCTGCCAATGAAGAGACTTTTAGCTCTGCACCAGTAGAAACTGAGAGTGGAGTTAGCACTACTACGACTCAGGTT
ATTGACTATGGTTTAATGTATTTTGTGGATGAGAAAGGTTTTTCTACAGGAATCATTGAGACTGATGATGAAGGAAAAAC
TGTAAAGGCAGAGGGGATGCAATACAACCCTGACGGTTCAGTCGCTACAAATGGCGGAAGAGGGCCAATTGTTTATGACG
TTACTACGGCCGAGTCTTCAACAACTTCCGCTGCCGCAACAAGGACACCAAACCCAGCTTTAAATTACCCAGATCAAGCC
TTTTATGATTTCCTGAGAGCACAAATTATATCGTCAAATACCAAGGTTATGGCCTCGCCAACTTTGATACTCTCGGAGAG
CCCCGAATCTATTTCTGGAGAGATTGGACGCTCAAAAGCGAATGAAGCCCAGGTATCAATTGGCGATAAAATTATTACAT
CATACAAAATAGTCCAAGACGCCAATGGCAATGCTTATTGTGAGGCTGGTTTTGATAATGCCGGTTTAACTTTGGGTGCC
ATGGTTAGCAAGATTGATGACAATGGCTTCGTTACATTTACACTTGAGCCTGATCTTTCAGCTGTTGTGGGCCAGCCAAT
CAGCACTGGAGGCAAATGTGGGGACGTTCGAACTGTTAACTCAAGGCGCTTGGAAACGGGTGCCGTTCGCGTTCGTGATG
GTCAAACATTGATTCTTACTGGTGTGATTAGTGATTCGGATACGGAGGTGGTCACAAAATGGCCAATTTTGGGAGATATA
CCTTTAATTGGGCAGTTCTTCAGGTCGTCGTCTGGATCGAGAAAGAAGAATGAGCTGGTGATCATGGTGACGCCTCGTAT
TATTAATGACGAGCGGGGGGGGACTTACGGCTATGGTTATCAACCTTCCACCAAAGCAACACGCAATTTAATTTATTCCC
AGTAG

Upstream 100 bases:

>100_bases
GAAGGATTCCCTCCTGAAGCCTGAAGCGCCCCTGGCTGGCAAGGAAATCGATCGATACCATCCGTGAAGATTGTGTGCCA
GATCAACCCTGGGTCGCCGT

Downstream 100 bases:

>100_bases
TTCAACACAATTAATCGTCTTTCATTTTGTGCTTTTAAAAGGCGAGGGGTGATAAAAACTTGACTGCTATGGGTAGCAAC
TGTTGAAACAAACCAGTTGC

Product: general (type II) secretion pathway protein D precursor

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MSRSQRLVPLLAALGLYGASISGGLMLPAAVKAQKQGTMELKLRRSQDSVEVIIEGVGPQPVVRQRQTGQDWEGRLTTQG
KPGLRRGPQQVSMPQLGLQSVSLAGSGESYQLNVEGVAGRPLMEPLVSADGRNLILSFPGLTAQAKQTGRLDLRTPGRVP
QASYVPPLQQRAVAPPLGDMAVGTMVLSNRSFVQVSGPSVTLTLNNAPAKDALMSLARLGGYGFIFVGDDSESGNTTGVG
TSADAPVNGGNKKVSMAFQNESYARALNGVLLASGLQGKLDGRTLLVGSAVASKTFGPQVSKVYRLNQASAASAADYLAS
LGAAISKVNIVSITSGEASSAGTSQLSNEVSQTSSKTTSIETYGASVGPLRGLTGTTDSRLQTITLVGDSQLVSVAESYL
KQIDLRSRQVALSVKILDVNLNNNSEMDNSFSVRFGDNLIVNDNGELLAAFGSNMPANEETFSSAPVETESGVSTTTTQV
IDYGLMYFVDEKGFSTGIIETDDEGKTVKAEGMQYNPDGSVATNGGRGPIVYDVTTAESSTTSAAATRTPNPALNYPDQA
FYDFLRAQIISSNTKVMASPTLILSESPESISGEIGRSKANEAQVSIGDKIITSYKIVQDANGNAYCEAGFDNAGLTLGA
MVSKIDDNGFVTFTLEPDLSAVVGQPISTGGKCGDVRTVNSRRLETGAVRVRDGQTLILTGVISDSDTEVVTKWPILGDI
PLIGQFFRSSSGSRKKNELVIMVTPRIINDERGGTYGYGYQPSTKATRNLIYSQ

Sequences:

>Translated_774_residues
MSRSQRLVPLLAALGLYGASISGGLMLPAAVKAQKQGTMELKLRRSQDSVEVIIEGVGPQPVVRQRQTGQDWEGRLTTQG
KPGLRRGPQQVSMPQLGLQSVSLAGSGESYQLNVEGVAGRPLMEPLVSADGRNLILSFPGLTAQAKQTGRLDLRTPGRVP
QASYVPPLQQRAVAPPLGDMAVGTMVLSNRSFVQVSGPSVTLTLNNAPAKDALMSLARLGGYGFIFVGDDSESGNTTGVG
TSADAPVNGGNKKVSMAFQNESYARALNGVLLASGLQGKLDGRTLLVGSAVASKTFGPQVSKVYRLNQASAASAADYLAS
LGAAISKVNIVSITSGEASSAGTSQLSNEVSQTSSKTTSIETYGASVGPLRGLTGTTDSRLQTITLVGDSQLVSVAESYL
KQIDLRSRQVALSVKILDVNLNNNSEMDNSFSVRFGDNLIVNDNGELLAAFGSNMPANEETFSSAPVETESGVSTTTTQV
IDYGLMYFVDEKGFSTGIIETDDEGKTVKAEGMQYNPDGSVATNGGRGPIVYDVTTAESSTTSAAATRTPNPALNYPDQA
FYDFLRAQIISSNTKVMASPTLILSESPESISGEIGRSKANEAQVSIGDKIITSYKIVQDANGNAYCEAGFDNAGLTLGA
MVSKIDDNGFVTFTLEPDLSAVVGQPISTGGKCGDVRTVNSRRLETGAVRVRDGQTLILTGVISDSDTEVVTKWPILGDI
PLIGQFFRSSSGSRKKNELVIMVTPRIINDERGGTYGYGYQPSTKATRNLIYSQ
>Mature_773_residues
SRSQRLVPLLAALGLYGASISGGLMLPAAVKAQKQGTMELKLRRSQDSVEVIIEGVGPQPVVRQRQTGQDWEGRLTTQGK
PGLRRGPQQVSMPQLGLQSVSLAGSGESYQLNVEGVAGRPLMEPLVSADGRNLILSFPGLTAQAKQTGRLDLRTPGRVPQ
ASYVPPLQQRAVAPPLGDMAVGTMVLSNRSFVQVSGPSVTLTLNNAPAKDALMSLARLGGYGFIFVGDDSESGNTTGVGT
SADAPVNGGNKKVSMAFQNESYARALNGVLLASGLQGKLDGRTLLVGSAVASKTFGPQVSKVYRLNQASAASAADYLASL
GAAISKVNIVSITSGEASSAGTSQLSNEVSQTSSKTTSIETYGASVGPLRGLTGTTDSRLQTITLVGDSQLVSVAESYLK
QIDLRSRQVALSVKILDVNLNNNSEMDNSFSVRFGDNLIVNDNGELLAAFGSNMPANEETFSSAPVETESGVSTTTTQVI
DYGLMYFVDEKGFSTGIIETDDEGKTVKAEGMQYNPDGSVATNGGRGPIVYDVTTAESSTTSAAATRTPNPALNYPDQAF
YDFLRAQIISSNTKVMASPTLILSESPESISGEIGRSKANEAQVSIGDKIITSYKIVQDANGNAYCEAGFDNAGLTLGAM
VSKIDDNGFVTFTLEPDLSAVVGQPISTGGKCGDVRTVNSRRLETGAVRVRDGQTLILTGVISDSDTEVVTKWPILGDIP
LIGQFFRSSSGSRKKNELVIMVTPRIINDERGGTYGYGYQPSTKATRNLIYSQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=74, Percent_Identity=44.5945945945946, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 81393; Mature: 81262

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSQRLVPLLAALGLYGASISGGLMLPAAVKAQKQGTMELKLRRSQDSVEVIIEGVGPQ
CCCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCC
PVVRQRQTGQDWEGRLTTQGKPGLRRGPQQVSMPQLGLQSVSLAGSGESYQLNVEGVAGR
HHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCC
PLMEPLVSADGRNLILSFPGLTAQAKQTGRLDLRTPGRVPQASYVPPLQQRAVAPPLGDM
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCH
AVGTMVLSNRSFVQVSGPSVTLTLNNAPAKDALMSLARLGGYGFIFVGDDSESGNTTGVG
HEEEEEECCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCC
TSADAPVNGGNKKVSMAFQNESYARALNGVLLASGLQGKLDGRTLLVGSAVASKTFGPQV
CCCCCCCCCCCEEEEEEECCCHHHHHHCCEEEECCCCCCCCCCEEEEECHHHHCCCCCCH
SKVYRLNQASAASAADYLASLGAAISKVNIVSITSGEASSAGTSQLSNEVSQTSSKTTSI
HHHEECCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEE
ETYGASVGPLRGLTGTTDSRLQTITLVGDSQLVSVAESYLKQIDLRSRQVALSVKILDVN
EECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEE
LNNNSEMDNSFSVRFGDNLIVNDNGELLAAFGSNMPANEETFSSAPVETESGVSTTTTQV
ECCCCCCCCEEEEEECCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHH
IDYGLMYFVDEKGFSTGIIETDDEGKTVKAEGMQYNPDGSVATNGGRGPIVYDVTTAESS
HHCCEEEEEECCCCCCCCEEECCCCCEEEECCEEECCCCCEEECCCCCCEEEEEECCCCC
TTSAAATRTPNPALNYPDQAFYDFLRAQIISSNTKVMASPTLILSESPESISGEIGRSKA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCHHCCCCCCCCC
NEAQVSIGDKIITSYKIVQDANGNAYCEAGFDNAGLTLGAMVSKIDDNGFVTFTLEPDLS
CCCEEECCCEEEEEEEEEECCCCCEEEECCCCCCCEEHHHHHHHCCCCCEEEEEECCCCH
AVVGQPISTGGKCGDVRTVNSRRLETGAVRVRDGQTLILTGVISDSDTEVVTKWPILGDI
HHHCCCCCCCCCCCCEEECCCCEEECCEEEEECCCEEEEEEEECCCCCEEEEECCCCCCC
PLIGQFFRSSSGSRKKNELVIMVTPRIINDERGGTYGYGYQPSTKATRNLIYSQ
HHHHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SRSQRLVPLLAALGLYGASISGGLMLPAAVKAQKQGTMELKLRRSQDSVEVIIEGVGPQ
CCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCC
PVVRQRQTGQDWEGRLTTQGKPGLRRGPQQVSMPQLGLQSVSLAGSGESYQLNVEGVAGR
HHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCC
PLMEPLVSADGRNLILSFPGLTAQAKQTGRLDLRTPGRVPQASYVPPLQQRAVAPPLGDM
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCH
AVGTMVLSNRSFVQVSGPSVTLTLNNAPAKDALMSLARLGGYGFIFVGDDSESGNTTGVG
HEEEEEECCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCC
TSADAPVNGGNKKVSMAFQNESYARALNGVLLASGLQGKLDGRTLLVGSAVASKTFGPQV
CCCCCCCCCCCEEEEEEECCCHHHHHHCCEEEECCCCCCCCCCEEEEECHHHHCCCCCCH
SKVYRLNQASAASAADYLASLGAAISKVNIVSITSGEASSAGTSQLSNEVSQTSSKTTSI
HHHEECCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEE
ETYGASVGPLRGLTGTTDSRLQTITLVGDSQLVSVAESYLKQIDLRSRQVALSVKILDVN
EECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEE
LNNNSEMDNSFSVRFGDNLIVNDNGELLAAFGSNMPANEETFSSAPVETESGVSTTTTQV
ECCCCCCCCEEEEEECCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHH
IDYGLMYFVDEKGFSTGIIETDDEGKTVKAEGMQYNPDGSVATNGGRGPIVYDVTTAESS
HHCCEEEEEECCCCCCCCEEECCCCCEEEECCEEECCCCCEEECCCCCCEEEEEECCCCC
TTSAAATRTPNPALNYPDQAFYDFLRAQIISSNTKVMASPTLILSESPESISGEIGRSKA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCHHCCCCCCCCC
NEAQVSIGDKIITSYKIVQDANGNAYCEAGFDNAGLTLGAMVSKIDDNGFVTFTLEPDLS
CCCEEECCCEEEEEEEEEECCCCCEEEECCCCCCCEEHHHHHHHCCCCCEEEEEECCCCH
AVVGQPISTGGKCGDVRTVNSRRLETGAVRVRDGQTLILTGVISDSDTEVVTKWPILGDI
HHHCCCCCCCCCCCCEEECCCCEEECCEEEEECCCEEEEEEEECCCCCEEEEECCCCCCC
PLIGQFFRSSSGSRKKNELVIMVTPRIINDERGGTYGYGYQPSTKATRNLIYSQ
HHHHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]