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The map label for this gene is menF [H]

Identifier: 33864325

GI number: 33864325

Start: 2169215

End: 2170636

Strand: Direct

Name: menF [H]

Synonym: PMT2060

Alternate gene names: 33864325

Gene position: 2169215-2170636 (Clockwise)

Preceding gene: 33864320

Following gene: 33864328

Centisome position: 89.98

GC content: 57.74

Gene sequence:

>1422_bases
ATGAAAGCTGACCGTAGTTTCAGCAACCTTTTAACTGCCGCATCCCGGGGCTGGTGCGAGCGCAAGGGCGATGAAGGTGT
CCTCAGCCTGGCCCTGCCTCTCGACGAGGTGGATCCCCTGTGCCACCTGCCCTTACTAGCCGATGAGCATCCCTTCCAGT
TTCTCTGGGATAGTGCACCAGGCCTTTGCTTTGCAGCAGCTGGTCAGTGCCAAAGCCTTGATCTTGCAGGCCCCAGACGC
TTTGAGCTAGCCCAACGATTCAGCGATACAACCCTGGCACGCCTCACAGACGCGACGCCCAACACCCCTGCCCAGGCTCG
AGCCAAAATCCTGCTGGCCTTTTCCTTTTTCGACCATCCCGCAGAACGACAACGCAGCCAGATCCACACCAGTGCAGTTC
AAGCGGTACTGCCACGCTGGCAACTCAGCCGCCAGGGTCGACACGGTTGGCTGCGTCTCAATGGCGTAATCACCCAGCAA
GCAGAAGCGCGCGACCTAGCAGAACAGCTTTGGCTGATGGCGGAAAACCTGCTCGACCCCAATCACAATTCAAAAGATTT
CTGGCCCAGCAGCGTCCGCGGCACCACGGCACTCCAGCCCTGGCAGGAGTGTTACAGACCAGCTCTCAAACGCGGACTGG
ATTTAGTGAGCAGCGGTGAGCTCAACAAATTGGTGCTGGCTGTGCGCCAATCCATTCAGCTCCAAGCACCCTTAAACCCG
CTGCCAGTACTGGCCAAGCTACGTCAACAACAATCAGGTAGCTGCCGCTTCTTATGGCGAAGAACTCACGAGGATGCCTT
CTTTGGAGCTTCGCCTGAACGACTACTCAGCCTGCAAGGGAATCAGCTACGCACCGACGCTCTAGCCGGCACTGCTGATC
GCAATGACGATGGACAGGGCCTACTCCGCTCGGAAAAGGACCGTCGCGAGCATGAACTTGTCGTTGCCTCAATTACCAGT
CAGCTGTGCAATCAAGGACTGACACCACGGCGACCACGGAACCCCCAGCTCGCTCGTCATGGCCAACTGGTGCATCTGCA
TACACCGATTACAGCCAATGCCATGGGTCACACATCCCTCAATCTCGCCAAAATTCTCCATCCAACTCCGGCGGTCGCGG
GTCTGCCTTGCCGAGAGGCGATGGCCTGGCTGCGCACATTGGAACCTTTTGAACGTGGCAGCTATGCGGCGCCGATTGGC
TGGATCGACAGCCAAGGCGACACGGAACTGCGCGTAGCCATCCGTTGTGGCCACGCCAGAGGTTCAGTGCTTGATCTCAC
TGCTGGAGCTGGTTTGGTCCGAGGGTCTGTGGTCGAACGCGAATTACAAGAAGTTGGCATGAAGCTGTCTGTACTGGCCG
ACCAACTCGATCTGGATGCCAAGGATCACCCAAGACTGACCAGCAAACGCGCAATCACTTGA

Upstream 100 bases:

>100_bases
AGGGATGCAACATCCTGCTGGTCTGGCATGGGAAAGAAGCCTGGAAACCAGGGAAAGGCCCATACATTTGAGCAGGACCC
GCGCTCAACTCCGATCCCGG

Downstream 100 bases:

>100_bases
TCAGCCAGGGGAACCCCCTTGAGGCGCTCAACCTCTCTAATGCCAGTAGGACTGGTGACATTGATCTCACTCAGCAGGCC
ATCAATCACATCAATTCCCA

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKADRSFSNLLTAASRGWCERKGDEGVLSLALPLDEVDPLCHLPLLADEHPFQFLWDSAPGLCFAAAGQCQSLDLAGPRR
FELAQRFSDTTLARLTDATPNTPAQARAKILLAFSFFDHPAERQRSQIHTSAVQAVLPRWQLSRQGRHGWLRLNGVITQQ
AEARDLAEQLWLMAENLLDPNHNSKDFWPSSVRGTTALQPWQECYRPALKRGLDLVSSGELNKLVLAVRQSIQLQAPLNP
LPVLAKLRQQQSGSCRFLWRRTHEDAFFGASPERLLSLQGNQLRTDALAGTADRNDDGQGLLRSEKDRREHELVVASITS
QLCNQGLTPRRPRNPQLARHGQLVHLHTPITANAMGHTSLNLAKILHPTPAVAGLPCREAMAWLRTLEPFERGSYAAPIG
WIDSQGDTELRVAIRCGHARGSVLDLTAGAGLVRGSVVERELQEVGMKLSVLADQLDLDAKDHPRLTSKRAIT

Sequences:

>Translated_473_residues
MKADRSFSNLLTAASRGWCERKGDEGVLSLALPLDEVDPLCHLPLLADEHPFQFLWDSAPGLCFAAAGQCQSLDLAGPRR
FELAQRFSDTTLARLTDATPNTPAQARAKILLAFSFFDHPAERQRSQIHTSAVQAVLPRWQLSRQGRHGWLRLNGVITQQ
AEARDLAEQLWLMAENLLDPNHNSKDFWPSSVRGTTALQPWQECYRPALKRGLDLVSSGELNKLVLAVRQSIQLQAPLNP
LPVLAKLRQQQSGSCRFLWRRTHEDAFFGASPERLLSLQGNQLRTDALAGTADRNDDGQGLLRSEKDRREHELVVASITS
QLCNQGLTPRRPRNPQLARHGQLVHLHTPITANAMGHTSLNLAKILHPTPAVAGLPCREAMAWLRTLEPFERGSYAAPIG
WIDSQGDTELRVAIRCGHARGSVLDLTAGAGLVRGSVVERELQEVGMKLSVLADQLDLDAKDHPRLTSKRAIT
>Mature_473_residues
MKADRSFSNLLTAASRGWCERKGDEGVLSLALPLDEVDPLCHLPLLADEHPFQFLWDSAPGLCFAAAGQCQSLDLAGPRR
FELAQRFSDTTLARLTDATPNTPAQARAKILLAFSFFDHPAERQRSQIHTSAVQAVLPRWQLSRQGRHGWLRLNGVITQQ
AEARDLAEQLWLMAENLLDPNHNSKDFWPSSVRGTTALQPWQECYRPALKRGLDLVSSGELNKLVLAVRQSIQLQAPLNP
LPVLAKLRQQQSGSCRFLWRRTHEDAFFGASPERLLSLQGNQLRTDALAGTADRNDDGQGLLRSEKDRREHELVVASITS
QLCNQGLTPRRPRNPQLARHGQLVHLHTPITANAMGHTSLNLAKILHPTPAVAGLPCREAMAWLRTLEPFERGSYAAPIG
WIDSQGDTELRVAIRCGHARGSVLDLTAGAGLVRGSVVERELQEVGMKLSVLADQLDLDAKDHPRLTSKRAIT

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=267, Percent_Identity=35.2059925093633, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082077, Length=251, Percent_Identity=34.6613545816733, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52367; Mature: 52367

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADRSFSNLLTAASRGWCERKGDEGVLSLALPLDEVDPLCHLPLLADEHPFQFLWDSAP
CCCCHHHHHHHHHHHCCHHHCCCCCCEEEEECCHHHCCCHHCCCCCCCCCCCHHEECCCC
GLCFAAAGQCQSLDLAGPRRFELAQRFSDTTLARLTDATPNTPAQARAKILLAFSFFDHP
CEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHEEEEEEHHHCCH
AERQRSQIHTSAVQAVLPRWQLSRQGRHGWLRLNGVITQQAEARDLAEQLWLMAENLLDP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCHHHHHHHHHHHHHHHHHCCC
NHNSKDFWPSSVRGTTALQPWQECYRPALKRGLDLVSSGELNKLVLAVRQSIQLQAPLNP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCH
LPVLAKLRQQQSGSCRFLWRRTHEDAFFGASPERLLSLQGNQLRTDALAGTADRNDDGQG
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEHHHHCCCCCCCCCCCC
LLRSEKDRREHELVVASITSQLCNQGLTPRRPRNPQLARHGQLVHLHTPITANAMGHTSL
CHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCH
NLAKILHPTPAVAGLPCREAMAWLRTLEPFERGSYAAPIGWIDSQGDTELRVAIRCGHAR
HHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCC
GSVLDLTAGAGLVRGSVVERELQEVGMKLSVLADQLDLDAKDHPRLTSKRAIT
CCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKADRSFSNLLTAASRGWCERKGDEGVLSLALPLDEVDPLCHLPLLADEHPFQFLWDSAP
CCCCHHHHHHHHHHHCCHHHCCCCCCEEEEECCHHHCCCHHCCCCCCCCCCCHHEECCCC
GLCFAAAGQCQSLDLAGPRRFELAQRFSDTTLARLTDATPNTPAQARAKILLAFSFFDHP
CEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHEEEEEEHHHCCH
AERQRSQIHTSAVQAVLPRWQLSRQGRHGWLRLNGVITQQAEARDLAEQLWLMAENLLDP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCHHHHHHHHHHHHHHHHHCCC
NHNSKDFWPSSVRGTTALQPWQECYRPALKRGLDLVSSGELNKLVLAVRQSIQLQAPLNP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCH
LPVLAKLRQQQSGSCRFLWRRTHEDAFFGASPERLLSLQGNQLRTDALAGTADRNDDGQG
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEHHHHCCCCCCCCCCCC
LLRSEKDRREHELVVASITSQLCNQGLTPRRPRNPQLARHGQLVHLHTPITANAMGHTSL
CHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCH
NLAKILHPTPAVAGLPCREAMAWLRTLEPFERGSYAAPIGWIDSQGDTELRVAIRCGHAR
HHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCC
GSVLDLTAGAGLVRGSVVERELQEVGMKLSVLADQLDLDAKDHPRLTSKRAIT
CCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]