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The map label for this gene is mraW [H]

Identifier: 33864313

GI number: 33864313

Start: 2159748

End: 2160680

Strand: Reverse

Name: mraW [H]

Synonym: PMT2048

Alternate gene names: 33864313

Gene position: 2160680-2159748 (Counterclockwise)

Preceding gene: 33864316

Following gene: 33864312

Centisome position: 89.62

GC content: 58.31

Gene sequence:

>933_bases
ATGCCCGATTCGTCCATTAATTCGGATTCAGGTTTCAAGCATCTGTCCGTAATGGAAGATGCCGTGTCGCAGCCTGTCGC
TGCTTTGCCTGCAGAGCTGCTCGTTGGCGGGTTGATCATTGATGCCACTCTGGGCGGCGGCGGCCATAGTGCTCTGTTGC
TGGAACGTCATCCAGATCTCCGCCTGATCGGTTTGGACCAAGACCCCACGGCTCGTGCGGCGGCGGCTGAGCGACTGGCG
CTATTTGGAGATCGCGTCACGATCGTGGCGACTAATTTTGTCGATTTCAGCCCCGCCGAGCCGGCAGTGGTGGTGATGGC
TGACTTGGGAGTGAGCAGTCCCCAACTGGATGTGGCGCAAAGAGGTTTTAGCTTTCGCCTCGATGGTCCGCTCGACATGC
GCATGAACCCCCAGGTGGGCGAAACGGCGGCTGAGCTGATTGGGCGTCTCGAAGAAACAGAGCTGGCAGATCTCATCTAT
GCCTATGGCGAGGAGCGCTTGTCCCGACGCATCGCCCGGCGCATTAAGCACGATCTGGCAGAGCAAGGACCCTATGCCGG
CACCGCCGCTTTGGCTTATGCGGTGGCTGGTTGCTATCCGCCGAAGGCCCGTCATGGTCGTATCCATCCAGCCACCCGTA
CTTTTCAGGCCCTGCGCATTGCTGTGAATAATGAATTGGATGCCTTAGATCGCTTCTTGCAAAAGGCCCCTGACTGGTTA
GTCCCAGGTGGTTTGCTCGCAGTGATCAGCTTTCATTCTCTTGAAGACCGTCGCGTCAAGACTGCTTTCTTGCGTGATCA
GCGCCTGGAGCGGCTAACCCGTAAGCCTTTGGTGGCCAGCGAGATGGAGATCGCTGCCAATCCACGCAGCCGTAGTGCAA
AGTGTCGATTCGCTCGCCGCTTGCCTCAAGCGACCACTGCCGATGCCCTCTGA

Upstream 100 bases:

>100_bases
ATGGGGGCCGAAGTTGACGACCATTGGCTCCGTACGCGTTTCCAGCTGCGACATGGGGCCTTGAGTTGCAGAGCGTTAGC
GGGATCTTAGGAAGGAACCC

Downstream 100 bases:

>100_bases
TCCAATCTGGTGGTGGGCTTTTGGAGTGCAACTGCTAGCTCTCCCTGGAGCATTGTTGCCGGTGTTACCGAGTTTGTTGT
GGTTGCCACTCGGTGCTGGT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPDSSINSDSGFKHLSVMEDAVSQPVAALPAELLVGGLIIDATLGGGGHSALLLERHPDLRLIGLDQDPTARAAAAERLA
LFGDRVTIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAQRGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIY
AYGEERLSRRIARRIKHDLAEQGPYAGTAALAYAVAGCYPPKARHGRIHPATRTFQALRIAVNNELDALDRFLQKAPDWL
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARRLPQATTADAL

Sequences:

>Translated_310_residues
MPDSSINSDSGFKHLSVMEDAVSQPVAALPAELLVGGLIIDATLGGGGHSALLLERHPDLRLIGLDQDPTARAAAAERLA
LFGDRVTIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAQRGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIY
AYGEERLSRRIARRIKHDLAEQGPYAGTAALAYAVAGCYPPKARHGRIHPATRTFQALRIAVNNELDALDRFLQKAPDWL
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARRLPQATTADAL
>Mature_309_residues
PDSSINSDSGFKHLSVMEDAVSQPVAALPAELLVGGLIIDATLGGGGHSALLLERHPDLRLIGLDQDPTARAAAAERLAL
FGDRVTIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAQRGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIYA
YGEERLSRRIARRIKHDLAEQGPYAGTAALAYAVAGCYPPKARHGRIHPATRTFQALRIAVNNELDALDRFLQKAPDWLV
PGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARRLPQATTADAL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=346, Percent_Identity=35.8381502890173, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI165377202, Length=177, Percent_Identity=36.7231638418079, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=40.8805031446541, Blast_Score=189, Evalue=3e-49,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=32.02416918429, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=32.02416918429, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 33687; Mature: 33556

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSSINSDSGFKHLSVMEDAVSQPVAALPAELLVGGLIIDATLGGGGHSALLLERHPDL
CCCCCCCCCCCCHHHHHHHHHHHCCHHHCCHHHHHCCEEEEEEECCCCCEEEEEECCCCE
RLIGLDQDPTARAAAAERLALFGDRVTIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAQ
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHH
RGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIYAYGEERLSRRIARRIKHDLA
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGPYAGTAALAYAVAGCYPPKARHGRIHPATRTFQALRIAVNNELDALDRFLQKAPDWL
HCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
LPQATTADAL
CCCCCCCCCC
>Mature Secondary Structure 
PDSSINSDSGFKHLSVMEDAVSQPVAALPAELLVGGLIIDATLGGGGHSALLLERHPDL
CCCCCCCCCCCHHHHHHHHHHHCCHHHCCHHHHHCCEEEEEEECCCCCEEEEEECCCCE
RLIGLDQDPTARAAAAERLALFGDRVTIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAQ
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHH
RGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIYAYGEERLSRRIARRIKHDLA
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGPYAGTAALAYAVAGCYPPKARHGRIHPATRTFQALRIAVNNELDALDRFLQKAPDWL
HCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
LPQATTADAL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA