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The map label for this gene is gltA [H]

Identifier: 33864286

GI number: 33864286

Start: 2134073

End: 2135287

Strand: Reverse

Name: gltA [H]

Synonym: PMT2021

Alternate gene names: 33864286

Gene position: 2135287-2134073 (Counterclockwise)

Preceding gene: 33864287

Following gene: 33864285

Centisome position: 88.57

GC content: 54.9

Gene sequence:

>1215_bases
TTGCCAAGGAGTTGTCGAGATGCGGTGGGGCAGGAGCAGGTTGGGGAGATTCGCCACGATCGCAGTGGCATCAGCTTTCG
TCCGGGGCTGGAGGGAGTGCCAGTAACCCAATCCGCCATCTGTGATATCGATGGACTGCAGGGGCGGCTTGCTTATCGGG
GTTATCCAATTGCGGAGCTCTCAGCGCATAGCAGCTTTTTGGAAACCACCTATCTGTTGATCTGGGGCGATCTGCCAAGC
CCACAGCAACTTCGTGATTTCGAAGCTGAGGTTCAGATGCACCGTCGCGTGAGTTTCCGGGTACGCGACATGATGAAGTG
CTTCCCAGCTTCGGGGCACCCGATGGATGCACTTCAGTCGAGTGCGGCATCCCTGGGGCTGTTCTATTCCCGTCGAGCCA
TCGATGACCCGAAGTACGTCTATAACGCCGTGGTGAGGCTGATCGCCAAGATTCCCACGATGGTGGCGGCCTTCAAGTTG
ATTCGCAAAGGGCAAGATCCCATTCAGCCTCGTGACGACCTGGCCTACTCAGCCAATTTCCTCTACATGCTCACCGAGCG
TGAACCGGACCCTTTGGCGGCAAGGGTTCTTGATCGCTGCTTGATCCTGCATGCGGAACACAGTCTTAATGCCAGTACTT
TTAGTGCTCGAGTGACCGCCAGTACGCTCACAGATCCTTATGCAGTGGTGGCTTCCGCAGTGGGCACCCTTGCAGGACCC
CTACATGGTGGTGCCAATGAGGATGTTTTGGCGATGCTTGAAGAGATCGGCAGCCCAGAGCATGCTGGTGATTACTTGGA
TGAAGCGATTGCTTCTAAGCGCAAGGTGATGGGCTTTGGTCATAGGGAATATCGGGTCAAGGATCCTCGCGCTGTGATCC
TTCAGGGTCTTGCAGAGGAGTTGTTTGCCCGATTCGGTACCGATGAGATGTACGACGTGGCCAAGGCGTTGGAGCAGGCG
GCTGCCACCCGTTTAGGCCCTAAAGGGATCTATCCGAATGTGGACTTCTATTCCGGTCTTGTGTATCGCAAGCTTGGAAT
TCCACGTGATCTTTTTACTCCTGTGTTTGCTATCTCAAGGGTGGCTGGTTGGCTAGCTCATTGGCGAGAGCAACTTGGCG
CGAATCGGATTTTCAGACCTTCGCAGATTTACACCGGTCCCCAGAACAGAACTTGGATCCCTAGTGATGAGCGTTTCTCT
TCTACGGGCGCTTAA

Upstream 100 bases:

>100_bases
GCACATCTCAGATGGTCGCAGCAGAAACGAAGCCCTGCTGGCAGGGCAACGCTGCAGGTGTTGTTGAGGCCGTCGGTGTT
GTTGCCCTGTTCCGCTTAGG

Downstream 100 bases:

>100_bases
GTTGCAGCCAGCTCAGCCATCACCATGGTGACCACCTTTGCCACTACAACCTCAGGCTTGGTGAGTTCTGGCCTTGATCT
GGAGTTGAGCTTTAGTCAGT

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPRSCRDAVGQEQVGEIRHDRSGISFRPGLEGVPVTQSAICDIDGLQGRLAYRGYPIAELSAHSSFLETTYLLIWGDLPS
PQQLRDFEAEVQMHRRVSFRVRDMMKCFPASGHPMDALQSSAASLGLFYSRRAIDDPKYVYNAVVRLIAKIPTMVAAFKL
IRKGQDPIQPRDDLAYSANFLYMLTEREPDPLAARVLDRCLILHAEHSLNASTFSARVTASTLTDPYAVVASAVGTLAGP
LHGGANEDVLAMLEEIGSPEHAGDYLDEAIASKRKVMGFGHREYRVKDPRAVILQGLAEELFARFGTDEMYDVAKALEQA
AATRLGPKGIYPNVDFYSGLVYRKLGIPRDLFTPVFAISRVAGWLAHWREQLGANRIFRPSQIYTGPQNRTWIPSDERFS
STGA

Sequences:

>Translated_404_residues
MPRSCRDAVGQEQVGEIRHDRSGISFRPGLEGVPVTQSAICDIDGLQGRLAYRGYPIAELSAHSSFLETTYLLIWGDLPS
PQQLRDFEAEVQMHRRVSFRVRDMMKCFPASGHPMDALQSSAASLGLFYSRRAIDDPKYVYNAVVRLIAKIPTMVAAFKL
IRKGQDPIQPRDDLAYSANFLYMLTEREPDPLAARVLDRCLILHAEHSLNASTFSARVTASTLTDPYAVVASAVGTLAGP
LHGGANEDVLAMLEEIGSPEHAGDYLDEAIASKRKVMGFGHREYRVKDPRAVILQGLAEELFARFGTDEMYDVAKALEQA
AATRLGPKGIYPNVDFYSGLVYRKLGIPRDLFTPVFAISRVAGWLAHWREQLGANRIFRPSQIYTGPQNRTWIPSDERFS
STGA
>Mature_403_residues
PRSCRDAVGQEQVGEIRHDRSGISFRPGLEGVPVTQSAICDIDGLQGRLAYRGYPIAELSAHSSFLETTYLLIWGDLPSP
QQLRDFEAEVQMHRRVSFRVRDMMKCFPASGHPMDALQSSAASLGLFYSRRAIDDPKYVYNAVVRLIAKIPTMVAAFKLI
RKGQDPIQPRDDLAYSANFLYMLTEREPDPLAARVLDRCLILHAEHSLNASTFSARVTASTLTDPYAVVASAVGTLAGPL
HGGANEDVLAMLEEIGSPEHAGDYLDEAIASKRKVMGFGHREYRVKDPRAVILQGLAEELFARFGTDEMYDVAKALEQAA
ATRLGPKGIYPNVDFYSGLVYRKLGIPRDLFTPVFAISRVAGWLAHWREQLGANRIFRPSQIYTGPQNRTWIPSDERFSS
TGA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=405, Percent_Identity=28.8888888888889, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1786939, Length=381, Percent_Identity=38.0577427821522, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1786527, Length=370, Percent_Identity=33.7837837837838, Blast_Score=234, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17555174, Length=334, Percent_Identity=31.1377245508982, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319850, Length=377, Percent_Identity=25.7294429708223, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325257, Length=344, Percent_Identity=26.453488372093, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=382, Percent_Identity=24.6073298429319, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640124, Length=421, Percent_Identity=27.790973871734, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640126, Length=421, Percent_Identity=27.790973871734, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356863, Length=408, Percent_Identity=26.4705882352941, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 44836; Mature: 44704

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSCRDAVGQEQVGEIRHDRSGISFRPGLEGVPVTQSAICDIDGLQGRLAYRGYPIAEL
CCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCHHHH
SAHSSFLETTYLLIWGDLPSPQQLRDFEAEVQMHRRVSFRVRDMMKCFPASGHPMDALQS
HHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SAASLGLFYSRRAIDDPKYVYNAVVRLIAKIPTMVAAFKLIRKGQDPIQPRDDLAYSANF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCE
LYMLTEREPDPLAARVLDRCLILHAEHSLNASTFSARVTASTLTDPYAVVASAVGTLAGP
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEEHHHCCCHHHHHHHHHHHHHCC
LHGGANEDVLAMLEEIGSPEHAGDYLDEAIASKRKVMGFGHREYRVKDPRAVILQGLAEE
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LFARFGTDEMYDVAKALEQAAATRLGPKGIYPNVDFYSGLVYRKLGIPRDLFTPVFAISR
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VAGWLAHWREQLGANRIFRPSQIYTGPQNRTWIPSDERFSSTGA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRSCRDAVGQEQVGEIRHDRSGISFRPGLEGVPVTQSAICDIDGLQGRLAYRGYPIAEL
CCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCHHHH
SAHSSFLETTYLLIWGDLPSPQQLRDFEAEVQMHRRVSFRVRDMMKCFPASGHPMDALQS
HHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SAASLGLFYSRRAIDDPKYVYNAVVRLIAKIPTMVAAFKLIRKGQDPIQPRDDLAYSANF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCE
LYMLTEREPDPLAARVLDRCLILHAEHSLNASTFSARVTASTLTDPYAVVASAVGTLAGP
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEEHHHCCCHHHHHHHHHHHHHCC
LHGGANEDVLAMLEEIGSPEHAGDYLDEAIASKRKVMGFGHREYRVKDPRAVILQGLAEE
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LFARFGTDEMYDVAKALEQAAATRLGPKGIYPNVDFYSGLVYRKLGIPRDLFTPVFAISR
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VAGWLAHWREQLGANRIFRPSQIYTGPQNRTWIPSDERFSSTGA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]