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The map label for this gene is ndhD [H]

Identifier: 33864275

GI number: 33864275

Start: 2123940

End: 2125631

Strand: Direct

Name: ndhD [H]

Synonym: PMT2010

Alternate gene names: 33864275

Gene position: 2123940-2125631 (Clockwise)

Preceding gene: 33864274

Following gene: 33864276

Centisome position: 88.1

GC content: 53.72

Gene sequence:

>1692_bases
GTGCTGGAGTTTGCCATAAGCGCACCCTTGGACACAGGTGCCGAGCTGCTGAGTGATCAAGTGAGCGCTAACTTCCCCTG
GCTGAGCCTCTCAATTCTGTTCCCGATTGTGGGGGCCTTTCTGGTGCCGTTCATCCCTGATGAAGGGGAGGGTAAGCAGG
TTCGTTGGTTTGCACTCGGCATCGCCCTGGTGACCTTTCTGATCACCGTGGCTGCCTACCTATACGGCTATGACCCCAGT
CTGAGCGGCTTACAACTATCCGAACGGGTGAGCTGGCTACCTGATCTGGGCCTCACCTGGGCCGTCGGAGCAGATGGCAT
CTCGATGCCACTCATTCTGCTCACCAGTTTCATCACAGCACTGGCAGTCCTGGCAGCCTGGCCAGTGACGTTCAAACCCA
AGCTGTTCTTTTTCCTGATACTGGCAATGGATGGCGGCCAGATCGCAGTTTTTGCCGTTCAAGACATGCTGCTGTTCTTC
CTGGCCTGGGAACTGGAATTGTTGCCGGTCTACCTGCTGCTAGCGATCTGGGGTGGCAAAAAAAGGCAATATGCAGCCAC
AAAATTCATCATTTATACCGCTGGCAGTTCGCTGTTCATTCTGCTGGTGGCCCTAGCCATGGGGTTCTTTGGCGGAGGCA
CACCCAACTTCGAATACACCAACCTCGCCCAACAAAGCTTTGGGACAGGTTTCCAGTTGCTCTGTTACGCCGGACTGCTC
ATTGCATTCGGCGTCAAGCTGCCAATTGTGCCTCTACACACCTGGCTACCAGATGCCCATGGAGAAGCAACAGCGCCAGT
TCACATGCTGCTTGCAGGCATCCTGCTGAAGATGGGCGGCTATGCCTTGATGCGCTTTAACGCTCAACTGCTACCTGAAG
CCCATGCCCAGTTCGCCCCACTTCTCATCGTGCTTGGGGTAGTCAACATCATCTATGCCGCTCTCACCTCCTTCGCCCAG
CGCAACCTCAAACGCAAGATCGCCTATAGCTCAATTAGCCACATGGGCTTTGTACTGATCGGCATTGGCAGCTTCAGTGC
CCTAGGAACGAGTGGCGCCATGCTGCAGATGATCAGTCATGGTCTAATCGGCGCCAGTCTGTTCTTCCTTGTAGGAGCCA
CCTACGACCGCACCCATACCTTGCAACTTGAGGAGATGGGAGGGGTGGGTCAAAAGATGCGAATCATGTTCGCCCTATGG
ACTGTCTGTGCACTCGCCTCTCTCGCTCTGCCTGGCATGAGTGGTTTCGTTTCTGAATTAATGGTGTTCGTGGGATTTGC
TACCGATGAGGCCTACACGCTGACATTCCGAATTGTGATTGCGGGGCTTGCGGCAATCGGCGTCATCCTTACCCCCATCT
ATCTACTCTCAATGCTCAGAGAAATCTTCTTCGGCAAAGAGAATGACCAACTTGTCTCCCATACCAACCTGGTGGATGCG
GAACCAAGAGAGGTCTACATCATTAGCTGCTTACTCGTGCCGATCATTGGCATTGGCCTCTACCCCCGACTCATGACCGA
TAGCTATACCGCATCCATCCAAGAATTAGTAAAGCGAGATGATTTAGCCCTGCAACGCATCAAAAAACCCTCAGCATTGA
TGATTCGAAACACCACCATGACGCCTGCTGTGGTCTCATCCCCAAGGCTGCCGATCACTCAAACGCGATCGGAGCAGCTC
ACTAGGAAATAA

Upstream 100 bases:

>100_bases
CCTGTTAGCTGATAAACCAAATTGTGTGTTATCGCCTATCGATGCAGTGTCAGAACGCCAAGCATTTCTACACTCCGACC
AGTGCTGAAAAGGCCTGTCC

Downstream 100 bases:

>100_bases
GATCTGCAGCCTTTCTTTGCACAGCGACCCACCCTGAAGTCTCCAGCATTAATGATGAGCTAGTTGCTTTTTTAAGGGAT
TCGCCAGATCAGTTTCATCT

Product: NAD(P)H-quinone oxidoreductase subunit 4

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4; NDH-1, chain 4 [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MLEFAISAPLDTGAELLSDQVSANFPWLSLSILFPIVGAFLVPFIPDEGEGKQVRWFALGIALVTFLITVAAYLYGYDPS
LSGLQLSERVSWLPDLGLTWAVGADGISMPLILLTSFITALAVLAAWPVTFKPKLFFFLILAMDGGQIAVFAVQDMLLFF
LAWELELLPVYLLLAIWGGKKRQYAATKFIIYTAGSSLFILLVALAMGFFGGGTPNFEYTNLAQQSFGTGFQLLCYAGLL
IAFGVKLPIVPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRFNAQLLPEAHAQFAPLLIVLGVVNIIYAALTSFAQ
RNLKRKIAYSSISHMGFVLIGIGSFSALGTSGAMLQMISHGLIGASLFFLVGATYDRTHTLQLEEMGGVGQKMRIMFALW
TVCALASLALPGMSGFVSELMVFVGFATDEAYTLTFRIVIAGLAAIGVILTPIYLLSMLREIFFGKENDQLVSHTNLVDA
EPREVYIISCLLVPIIGIGLYPRLMTDSYTASIQELVKRDDLALQRIKKPSALMIRNTTMTPAVVSSPRLPITQTRSEQL
TRK

Sequences:

>Translated_563_residues
MLEFAISAPLDTGAELLSDQVSANFPWLSLSILFPIVGAFLVPFIPDEGEGKQVRWFALGIALVTFLITVAAYLYGYDPS
LSGLQLSERVSWLPDLGLTWAVGADGISMPLILLTSFITALAVLAAWPVTFKPKLFFFLILAMDGGQIAVFAVQDMLLFF
LAWELELLPVYLLLAIWGGKKRQYAATKFIIYTAGSSLFILLVALAMGFFGGGTPNFEYTNLAQQSFGTGFQLLCYAGLL
IAFGVKLPIVPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRFNAQLLPEAHAQFAPLLIVLGVVNIIYAALTSFAQ
RNLKRKIAYSSISHMGFVLIGIGSFSALGTSGAMLQMISHGLIGASLFFLVGATYDRTHTLQLEEMGGVGQKMRIMFALW
TVCALASLALPGMSGFVSELMVFVGFATDEAYTLTFRIVIAGLAAIGVILTPIYLLSMLREIFFGKENDQLVSHTNLVDA
EPREVYIISCLLVPIIGIGLYPRLMTDSYTASIQELVKRDDLALQRIKKPSALMIRNTTMTPAVVSSPRLPITQTRSEQL
TRK
>Mature_563_residues
MLEFAISAPLDTGAELLSDQVSANFPWLSLSILFPIVGAFLVPFIPDEGEGKQVRWFALGIALVTFLITVAAYLYGYDPS
LSGLQLSERVSWLPDLGLTWAVGADGISMPLILLTSFITALAVLAAWPVTFKPKLFFFLILAMDGGQIAVFAVQDMLLFF
LAWELELLPVYLLLAIWGGKKRQYAATKFIIYTAGSSLFILLVALAMGFFGGGTPNFEYTNLAQQSFGTGFQLLCYAGLL
IAFGVKLPIVPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRFNAQLLPEAHAQFAPLLIVLGVVNIIYAALTSFAQ
RNLKRKIAYSSISHMGFVLIGIGSFSALGTSGAMLQMISHGLIGASLFFLVGATYDRTHTLQLEEMGGVGQKMRIMFALW
TVCALASLALPGMSGFVSELMVFVGFATDEAYTLTFRIVIAGLAAIGVILTPIYLLSMLREIFFGKENDQLVSHTNLVDA
EPREVYIISCLLVPIIGIGLYPRLMTDSYTASIQELVKRDDLALQRIKKPSALMIRNTTMTPAVVSSPRLPITQTRSEQL
TRK

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=377, Percent_Identity=30.7692307692308, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI251831117, Length=170, Percent_Identity=28.8235294117647, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788613, Length=508, Percent_Identity=39.5669291338583, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1788831, Length=298, Percent_Identity=30.2013422818792, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788827, Length=285, Percent_Identity=24.2105263157895, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788614, Length=472, Percent_Identity=26.271186440678, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI2367154, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693160, Length=382, Percent_Identity=26.1780104712042, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788829, Length=269, Percent_Identity=29.368029739777, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 61321; Mature: 61321

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFAISAPLDTGAELLSDQVSANFPWLSLSILFPIVGAFLVPFIPDEGEGKQVRWFALG
CCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHH
IALVTFLITVAAYLYGYDPSLSGLQLSERVSWLPDLGLTWAVGADGISMPLILLTSFITA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHH
LAVLAAWPVTFKPKLFFFLILAMDGGQIAVFAVQDMLLFFLAWELELLPVYLLLAIWGGK
HHHHHHCCCCCCCHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KRQYAATKFIIYTAGSSLFILLVALAMGFFGGGTPNFEYTNLAQQSFGTGFQLLCYAGLL
CCCEEHEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
IAFGVKLPIVPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRFNAQLLPEAHAQFAP
HHHCCCCCCEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCHHHHHHHH
LLIVLGVVNIIYAALTSFAQRNLKRKIAYSSISHMGFVLIGIGSFSALGTSGAMLQMISH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHHHH
GLIGASLFFLVGATYDRTHTLQLEEMGGVGQKMRIMFALWTVCALASLALPGMSGFVSEL
HHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MVFVGFATDEAYTLTFRIVIAGLAAIGVILTPIYLLSMLREIFFGKENDQLVSHTNLVDA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
EPREVYIISCLLVPIIGIGLYPRLMTDSYTASIQELVKRDDLALQRIKKPSALMIRNTTM
CCCCEEHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
TPAVVSSPRLPITQTRSEQLTRK
CCHHHCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MLEFAISAPLDTGAELLSDQVSANFPWLSLSILFPIVGAFLVPFIPDEGEGKQVRWFALG
CCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHH
IALVTFLITVAAYLYGYDPSLSGLQLSERVSWLPDLGLTWAVGADGISMPLILLTSFITA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHH
LAVLAAWPVTFKPKLFFFLILAMDGGQIAVFAVQDMLLFFLAWELELLPVYLLLAIWGGK
HHHHHHCCCCCCCHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KRQYAATKFIIYTAGSSLFILLVALAMGFFGGGTPNFEYTNLAQQSFGTGFQLLCYAGLL
CCCEEHEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
IAFGVKLPIVPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRFNAQLLPEAHAQFAP
HHHCCCCCCEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHCCHHHHHHHH
LLIVLGVVNIIYAALTSFAQRNLKRKIAYSSISHMGFVLIGIGSFSALGTSGAMLQMISH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHHHH
GLIGASLFFLVGATYDRTHTLQLEEMGGVGQKMRIMFALWTVCALASLALPGMSGFVSEL
HHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MVFVGFATDEAYTLTFRIVIAGLAAIGVILTPIYLLSMLREIFFGKENDQLVSHTNLVDA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
EPREVYIISCLLVPIIGIGLYPRLMTDSYTASIQELVKRDDLALQRIKKPSALMIRNTTM
CCCCEEHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
TPAVVSSPRLPITQTRSEQLTRK
CCHHHCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA