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The map label for this gene is ndhF [H]

Identifier: 33864274

GI number: 33864274

Start: 2121839

End: 2123851

Strand: Direct

Name: ndhF [H]

Synonym: PMT2009

Alternate gene names: 33864274

Gene position: 2121839-2123851 (Clockwise)

Preceding gene: 33864273

Following gene: 33864275

Centisome position: 88.01

GC content: 55.19

Gene sequence:

>2013_bases
ATGAACTCGGCCGCCGACCTCGCCTGGTTAATCCCGGTGTTCCCCCTACTGGGAGCGATCGCCACAGGCCTGGGACTAAT
CAGTTTCAATCGCACCATTAACCGCCTACGTAAGCCGGTAGCTGTGCTGCTGCTCACCTCTCTAGGTGCTGCCGCCGTCT
TGAGCTATGCAGTCCTCGCAGAGCAGCTGACCGGTGCTGCACCTGTCGAACATCTCTTCATCTGGGCCAGCGCCGGCAGT
TTCAGTCTGCCAATGGGTTATGTGATTGATCCCCTTGGAGCAGTGATGCTTGCCCTGGTCACCACCATCGCTCTGCTGGT
GATGATCTACTCCCACGGCTACATGGCCCACGACAAGGGCTACGTGCGTTTCTTTACCTATCTAGCCCTATTCAGCAGCT
CGATGCTGGGCTTGATTATCAGCCCCAACCTTCTCGAAATCTACGTGTTTTGGGAGCTGGTAGGGATGTGCTCCTATCTC
CTGGTGGGCTTCTGGTACGACCGAGATGGTGCCGCCCATGCAGCTCAAAAGGCTTTTGTCGTCAACCGTGTGGGTGATTT
TGGCTTGCTCCTTGGCATTCTCGGAGTCTTCTGGGCAACGGGCAGTTTTGATTTCCATGGCATTGCTAATGGCTTGTCAG
ATGCCATCGCGAACGGCACCGTTCCAGGCTGGGCGGCATTGACCTTATGTCTATTGATCTTCATGGGGCCGATGGCCAAG
TCAGCTCAATTCCCCCTTCATGTTTGGCTTCCCGATGCCATGGAAGGGCCCACACCAATTTCAGCTCTCATCCATGCGGC
AACCATGGTGGCAGCAGGTGTCTTCCTCGTTGCCAGGCTAGAACCTCTCTACAGCCAGTTCCCCATGGTGGGTTTGGTGA
TCGCCGTGATCGGCACGATCACTTGTTTTCTGGGAGCGTCCATCGCGCTCACCCAGATGGACCTCAAGAAGGGACTGGCT
TACAGCACGGTGTCTCAACTTGGATACATGATGCTGGCGATGGGCTGCGGCGCTCCCGTAGCAGGAATGTTCCACCTGGT
GACCCATGCCTTTTTCAAGGCCATGCTTTTCCTTGGCTCAGGTTCAGTAATCCACGCCATGGAAGACGTTGTTGGTCACG
AGCCCATCCTGGCCCAAGACATGAGGCTGATGGGCGGCCTGCGCCAAAAAATGCCGATCACAGCCATCACCTTCCTGATC
GGCTGCATTGCCATAAGCGGTATTCCGCCTCTAGCAGGCTTCTGGAGCAAGGACGAAATTCTTGGACAAGCATTCACCAC
CTTCCCATTGCTCTGGGTTGTGGGCTTTCTGACAGCAGGGATGACGGCCTTCTACATGTTCCGCCTCTACTTCCTCACAT
TTGAAGGGCCATTCCGCGGAGACGACAAAACCCTTCAGGCAAATTTGATTGCTGCTGCGGGCAAAGGCAGTGAAGACGAG
CATCCTCATCAAGGCGGGGTCCTGCATGAGTCGCCCTGGTCCATGACTCTGCCATTAGCAGTCTTGGCAGTGCCCTCGAT
GCTGATTGGTCTACTCGGCACCCCCTGGAACAGCCGCTTTGCAGGCCTGCTCAACCCAGAGGAGGCTCTTGAAATGGCCG
CAACATTCAACTGGAGTGAATTCCTGCCACTCGCAGGTGCTTCAGTGGCGATCTCAACAGCAGGCATCAGCTTGGCTGTC
TTAGCTTATGCACTGCACCGCCTCGATCTCGAAGAACTATTTGCCAATCGTTTCCCTGTCATCAATACCTTCCTGGCCAA
CAAGTGGTATTTGGATGACATCAATGAAAAGATCTTCGTACGCGGCAGCCGCAAACTGGCTCGTGAAGTGCTTGAAGTGG
ATGCCAAGGTGGTTGACGGCGTGGTGAATCTTACCGGCCTACTCACGCTTGGAAGTGGCGAAGGTCTTAAGTACTTTGAA
ACTGGCCGCGCTCAGTTTTACGCCCTCATCGTTTTCGGTGGTGTCATCGCCTTGGTCGTGCTTTTCGGCGTCCTAGGCAG
TCCTGTTAGCTGA

Upstream 100 bases:

>100_bases
CATCGCTTCATCCCCATCGGCAGCGCGGCCTTCCTCTCCTCCAAGCTGGAAGGCCTGCTGAGCTGAATTGCCTACACTCC
CATGATCCTGCCTGGACCGG

Downstream 100 bases:

>100_bases
TAAACCAAATTGTGTGTTATCGCCTATCGATGCAGTGTCAGAACGCCAAGCATTTCTACACTCCGACCAGTGCTGAAAAG
GCCTGTCCGTGCTGGAGTTT

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MNSAADLAWLIPVFPLLGAIATGLGLISFNRTINRLRKPVAVLLLTSLGAAAVLSYAVLAEQLTGAAPVEHLFIWASAGS
FSLPMGYVIDPLGAVMLALVTTIALLVMIYSHGYMAHDKGYVRFFTYLALFSSSMLGLIISPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGVFWATGSFDFHGIANGLSDAIANGTVPGWAALTLCLLIFMGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLEPLYSQFPMVGLVIAVIGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGMFHLVTHAFFKAMLFLGSGSVIHAMEDVVGHEPILAQDMRLMGGLRQKMPITAITFLI
GCIAISGIPPLAGFWSKDEILGQAFTTFPLLWVVGFLTAGMTAFYMFRLYFLTFEGPFRGDDKTLQANLIAAAGKGSEDE
HPHQGGVLHESPWSMTLPLAVLAVPSMLIGLLGTPWNSRFAGLLNPEEALEMAATFNWSEFLPLAGASVAISTAGISLAV
LAYALHRLDLEELFANRFPVINTFLANKWYLDDINEKIFVRGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYFE
TGRAQFYALIVFGGVIALVVLFGVLGSPVS

Sequences:

>Translated_670_residues
MNSAADLAWLIPVFPLLGAIATGLGLISFNRTINRLRKPVAVLLLTSLGAAAVLSYAVLAEQLTGAAPVEHLFIWASAGS
FSLPMGYVIDPLGAVMLALVTTIALLVMIYSHGYMAHDKGYVRFFTYLALFSSSMLGLIISPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGVFWATGSFDFHGIANGLSDAIANGTVPGWAALTLCLLIFMGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLEPLYSQFPMVGLVIAVIGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGMFHLVTHAFFKAMLFLGSGSVIHAMEDVVGHEPILAQDMRLMGGLRQKMPITAITFLI
GCIAISGIPPLAGFWSKDEILGQAFTTFPLLWVVGFLTAGMTAFYMFRLYFLTFEGPFRGDDKTLQANLIAAAGKGSEDE
HPHQGGVLHESPWSMTLPLAVLAVPSMLIGLLGTPWNSRFAGLLNPEEALEMAATFNWSEFLPLAGASVAISTAGISLAV
LAYALHRLDLEELFANRFPVINTFLANKWYLDDINEKIFVRGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYFE
TGRAQFYALIVFGGVIALVVLFGVLGSPVS
>Mature_670_residues
MNSAADLAWLIPVFPLLGAIATGLGLISFNRTINRLRKPVAVLLLTSLGAAAVLSYAVLAEQLTGAAPVEHLFIWASAGS
FSLPMGYVIDPLGAVMLALVTTIALLVMIYSHGYMAHDKGYVRFFTYLALFSSSMLGLIISPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGVFWATGSFDFHGIANGLSDAIANGTVPGWAALTLCLLIFMGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLEPLYSQFPMVGLVIAVIGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGMFHLVTHAFFKAMLFLGSGSVIHAMEDVVGHEPILAQDMRLMGGLRQKMPITAITFLI
GCIAISGIPPLAGFWSKDEILGQAFTTFPLLWVVGFLTAGMTAFYMFRLYFLTFEGPFRGDDKTLQANLIAAAGKGSEDE
HPHQGGVLHESPWSMTLPLAVLAVPSMLIGLLGTPWNSRFAGLLNPEEALEMAATFNWSEFLPLAGASVAISTAGISLAV
LAYALHRLDLEELFANRFPVINTFLANKWYLDDINEKIFVRGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYFE
TGRAQFYALIVFGGVIALVVLFGVLGSPVS

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=387, Percent_Identity=37.7260981912145, Blast_Score=260, Evalue=3e-69,
Organism=Escherichia coli, GI1788614, Length=647, Percent_Identity=36.9397217928903, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1788829, Length=368, Percent_Identity=32.6086956521739, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI1788827, Length=405, Percent_Identity=29.8765432098765, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1788831, Length=348, Percent_Identity=29.8850574712644, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1788613, Length=370, Percent_Identity=25.9459459459459, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI2367154, Length=401, Percent_Identity=25.1870324189526, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI145693160, Length=325, Percent_Identity=25.5384615384615, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 71815; Mature: 71815

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAADLAWLIPVFPLLGAIATGLGLISFNRTINRLRKPVAVLLLTSLGAAAVLSYAVLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLTGAAPVEHLFIWASAGSFSLPMGYVIDPLGAVMLALVTTIALLVMIYSHGYMAHDKG
HHHCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
YVRFFTYLALFSSSMLGLIISPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFV
HHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGVFWATGSFDFHGIANGLSDAIANGTVPGWAALTLCLLIFMGPMAK
HHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLEPLYSQFPMVGLVIAVIGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGMFHLVTHAFFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEDVVGHEPILAQDMRLMGGLRQKMPITAITFLIGCIAISGIPPLAGFWSKDEI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LGQAFTTFPLLWVVGFLTAGMTAFYMFRLYFLTFEGPFRGDDKTLQANLIAAAGKGSEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEHEEEECCCCCCCC
HPHQGGVLHESPWSMTLPLAVLAVPSMLIGLLGTPWNSRFAGLLNPEEALEMAATFNWSE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHH
FLPLAGASVAISTAGISLAVLAYALHRLDLEELFANRFPVINTFLANKWYLDDINEKIFV
HCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEE
RGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYFETGRAQFYALIVFGGVIALVV
ECCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHH
LFGVLGSPVS
HHHHHCCCCC
>Mature Secondary Structure
MNSAADLAWLIPVFPLLGAIATGLGLISFNRTINRLRKPVAVLLLTSLGAAAVLSYAVLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLTGAAPVEHLFIWASAGSFSLPMGYVIDPLGAVMLALVTTIALLVMIYSHGYMAHDKG
HHHCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
YVRFFTYLALFSSSMLGLIISPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFV
HHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGVFWATGSFDFHGIANGLSDAIANGTVPGWAALTLCLLIFMGPMAK
HHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARLEPLYSQFPMVGLVIAVIGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGMFHLVTHAFFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEDVVGHEPILAQDMRLMGGLRQKMPITAITFLIGCIAISGIPPLAGFWSKDEI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LGQAFTTFPLLWVVGFLTAGMTAFYMFRLYFLTFEGPFRGDDKTLQANLIAAAGKGSEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEHEEEECCCCCCCC
HPHQGGVLHESPWSMTLPLAVLAVPSMLIGLLGTPWNSRFAGLLNPEEALEMAATFNWSE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHH
FLPLAGASVAISTAGISLAVLAYALHRLDLEELFANRFPVINTFLANKWYLDDINEKIFV
HCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEE
RGSRKLAREVLEVDAKVVDGVVNLTGLLTLGSGEGLKYFETGRAQFYALIVFGGVIALVV
ECCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHHHH
LFGVLGSPVS
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]