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The map label for this gene is trmB [H]

Identifier: 33864087

GI number: 33864087

Start: 1928221

End: 1928931

Strand: Direct

Name: trmB [H]

Synonym: PMT1820

Alternate gene names: 33864087

Gene position: 1928221-1928931 (Clockwise)

Preceding gene: 33864086

Following gene: 33864088

Centisome position: 79.98

GC content: 53.59

Gene sequence:

>711_bases
GTGCGCCAGCACGTCAATCCTCTCAGCCGTTTCTTCCAACTTCCAAGGCCACTGCCAAGCCCGGAAGAAATGTTTGCGCA
ATCCAGCCGCCCTCTCCATCTCGACATCGGTTGTGCTCGAGGTGGCTTCCTGTTAAGCCTGGCTCCACTTCAACCAGAGT
GGAATCATGTGGGTGTTGAGATTCGCCACCCGCTTGTGCTTTCAGCGGAAAGGGATCGCCAAGAACTCGAACTCGACAAC
CTCCGCTTCTTGTTCTGCAACGTCAATGTGAGCCTTGAGGAGTGGCTTGATGCCCTGCCTAGAGATCAGCTGCAATGGGT
GTCCATCCAGTTCCCAGACCCCTGGTTTAAACGTCGTCATCAGAAGCGCAGGGTGCTGCAACCCTCCCTGTTAATCGCCT
TAGCAACAGCCCTGCAACCAGGCCGTGAACTCTTTATCCAGAGTGATGTGCTGTCTGTTATCGAGCCGATGGTGATGCTG
ATTGAACAAAGCAACTGTTTCGAGCGTCCTAAAAATGATTCCCATGCCTGGCAAAAGGCCAACCCACTGCCCGTGCCGAC
GGAGCGAGAACGTTATGTGCTCGATCAGGGCCTACAGGTTTACCGCAGGCTCTACCAACGCAACGACCAACAAGCTCCTG
AACTGTCCAACCTCGAAGCTTTATGGCAACAGGTCGATAATCCAAGTAAAGAAGAACATAGCGACTGCTGA

Upstream 100 bases:

>100_bases
CCGGAGGCGTTGGCGCTACCCACATTCACGGTTACACGGCCTGCTGGGGTTTGCTTAGGCATGACCCCTCTTCCTCGGAA
CCAGCCAAATCGGTCTAAGT

Downstream 100 bases:

>100_bases
TGCCTAAGACTGCGGCCCCACAGCCCCTTCTACTGCGCTGGCAGGGACACATTCCCTCCTCTGAGGCGATGCAAATGCGC
CTGCAGTGGATTGCGGGGTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MRQHVNPLSRFFQLPRPLPSPEEMFAQSSRPLHLDIGCARGGFLLSLAPLQPEWNHVGVEIRHPLVLSAERDRQELELDN
LRFLFCNVNVSLEEWLDALPRDQLQWVSIQFPDPWFKRRHQKRRVLQPSLLIALATALQPGRELFIQSDVLSVIEPMVML
IEQSNCFERPKNDSHAWQKANPLPVPTERERYVLDQGLQVYRRLYQRNDQQAPELSNLEALWQQVDNPSKEEHSDC

Sequences:

>Translated_236_residues
MRQHVNPLSRFFQLPRPLPSPEEMFAQSSRPLHLDIGCARGGFLLSLAPLQPEWNHVGVEIRHPLVLSAERDRQELELDN
LRFLFCNVNVSLEEWLDALPRDQLQWVSIQFPDPWFKRRHQKRRVLQPSLLIALATALQPGRELFIQSDVLSVIEPMVML
IEQSNCFERPKNDSHAWQKANPLPVPTERERYVLDQGLQVYRRLYQRNDQQAPELSNLEALWQQVDNPSKEEHSDC
>Mature_236_residues
MRQHVNPLSRFFQLPRPLPSPEEMFAQSSRPLHLDIGCARGGFLLSLAPLQPEWNHVGVEIRHPLVLSAERDRQELELDN
LRFLFCNVNVSLEEWLDALPRDQLQWVSIQFPDPWFKRRHQKRRVLQPSLLIALATALQPGRELFIQSDVLSVIEPMVML
IEQSNCFERPKNDSHAWQKANPLPVPTERERYVLDQGLQVYRRLYQRNDQQAPELSNLEALWQQVDNPSKEEHSDC

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI167004078, Length=177, Percent_Identity=29.3785310734463, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1789330, Length=152, Percent_Identity=30.2631578947368, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320000, Length=188, Percent_Identity=32.4468085106383, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27652; Mature: 27652

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHVNPLSRFFQLPRPLPSPEEMFAQSSRPLHLDIGCARGGFLLSLAPLQPEWNHVGVE
CCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEE
IRHPLVLSAERDRQELELDNLRFLFCNVNVSLEEWLDALPRDQLQWVSIQFPDPWFKRRH
ECCCEEEECCCCHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCHHHHHHH
QKRRVLQPSLLIALATALQPGRELFIQSDVLSVIEPMVMLIEQSNCFERPKNDSHAWQKA
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHCC
NPLPVPTERERYVLDQGLQVYRRLYQRNDQQAPELSNLEALWQQVDNPSKEEHSDC
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRQHVNPLSRFFQLPRPLPSPEEMFAQSSRPLHLDIGCARGGFLLSLAPLQPEWNHVGVE
CCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEE
IRHPLVLSAERDRQELELDNLRFLFCNVNVSLEEWLDALPRDQLQWVSIQFPDPWFKRRH
ECCCEEEECCCCHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCHHHHHHH
QKRRVLQPSLLIALATALQPGRELFIQSDVLSVIEPMVMLIEQSNCFERPKNDSHAWQKA
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHCC
NPLPVPTERERYVLDQGLQVYRRLYQRNDQQAPELSNLEALWQQVDNPSKEEHSDC
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA