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The map label for this gene is cbiG/cobJ-fusion [H]

Identifier: 33864039

GI number: 33864039

Start: 1866577

End: 1868355

Strand: Direct

Name: cbiG/cobJ-fusion [H]

Synonym: PMT1772

Alternate gene names: 33864039

Gene position: 1866577-1868355 (Clockwise)

Preceding gene: 33864038

Following gene: 33864042

Centisome position: 77.42

GC content: 57.73

Gene sequence:

>1779_bases
TTGCAAAGGCTTGCAAGACGCCACCAGGTGGATCAACTGGCCCTTACCCCGCGTGCAGCAAACACTCTCGAGGAAACACC
TCAAAGCCTTGTAGTTGGCCCCGCCAGCGAAGTGCTGAGCAGAAACTGGCAGCCAAACGGGGTCTTGCTCATCGTGGGTG
CTATCGGCGCCGTGACTCGCTTAATTGCTCCATTACTCATTGGGAAGGACAAAGATCCCGCCGTGATCGTCCTTGATGCC
CATGGCAAACACGTCGTGCCATTGCTGGGCGGTCACCAGGCGGGCGCAGAACAACTTGCCATGGAACTGGCCGCAGAGCT
AGGCGGCCATGCTGTACTCACCGGAGACGCCAATAGCCAAAACCGCTTGGCCCTCGATAGTTTTGGCAAAGGCTGGGGCT
GGCGACGTAGCGGCAGCCGCGAGAACTGGAATCAGTTGATGCTGACCCAAGCCCAAGGGGGGAAGCTTTTGCTTCTGCAG
CATTCTGGTGCGACGTTTTGGCAAACAACTGCTGCTGCACAAGACGTTTTTGATGCCAATGCCAATGCTGATCAGGCTCG
GCCAGCCGAGCTAACCATCGGCCCACAAAGCAGCATGTCTTGCAGCTGGCATCCCGCCAGTCTATGGATTGGAATTGGCT
GCGAACGCGACACAAGCCTGAGCCTGCTTGAGCGCGCCGTGGCAGCTGCCCTGGCAGAGGCAGGCCTTGCCCCAGAGGCA
GTAGCAGGCCTTGCCAGCATTGACCGAAAGGCCGATGAAACCGCTCTGCTCGCTCTCGCGCAAGCAAAGGACTGGCCTGT
GCGCTTCTTCAACGCTGATGCCCTCGCAGAAGTTGACGTACCCACACCTTCTGCTGTGGTAGCAAAAGCTATGGGCACCT
CTTCCGTGGCCGAAGCAGCAGCACTACTCGCAGCTAGCAACAAAAGCACTTTGCTGCTAACCAAACAGATTCACCACGCC
CAAAACGCTGAACATGGTGCTACCACCATCGCCATCAGCGAAGCCAATCAACCTTTTGCACCCCAAAGAGGCGAATTGCA
TCTGATCGGAAGCGGTCCTGGAGATTTGGCCTTTCTCACCCACGATGCCCGTGCAGCACTAGCCCGCAGTGCCATATGGG
TTGGATACGACCTCTACCTCGATCTACTGGAACCGTTGCGCCGCCCAGATCAAGCCCGTCTGGACGGGCAACTCACTCGC
GAACGGGATCGCTGCCTTAAGGCCCTGGAGCTAGCCCAACAGGGAGCTCGAGTCGCCTTGATCTCTTCAGGAGACAGTGG
CATCTACGGCATGGCCGGCCTAGCCCTGGAACTATGGCTCACACAACCCCCTAGTGATCGGCCACAATTTCAGGTGCATC
CAGGCCTCTCAGCCCTGCAATTAGCCGCCGCCAAGGTAGGTGCCCCTCTGATGCACGATTTCTGCAGCGTGAGTCTCAGC
GATCGCCTCACTCCCTGGTCAAAGATTGAAACCCGCCTGAAAAGTGCAGCCAGCGGAGATTTCGTGGTAGCCCTTTACAA
CCCACGCTCTCAAGAACGCGACTGGCAGCTGACGCGTGCCATTGAACTCTTGCTGGAGCATCGAGCCCCCAGCACGCCAG
TGGTCGTAGCACGTCAACTTGGACGAGCGCAGGAAGACATACAGCTGCACACACTGCAGACGCTGCCAGTCAAAGAGATC
GACATGCTCACGATTGTGCTAATCGGCAATAGCAGCAGTCGTTTGCAAGACAATCATGTGGTGACACCAAGGGGATACCC
CGGAGCCGAACTGGCTTGA

Upstream 100 bases:

>100_bases
CTCCAACGTTCCCCTTGGCCAACCTGCCCTGACCCATCCCGACGCACCTAGCCCCGCTCTGCGCCTGGCTCTTGGTCTCT
CTGTCAGCGCCTGGCCCCTT

Downstream 100 bases:

>100_bases
AAACATGTCAACTTCAATCAAGCTGAACGTTGTTTCAAGGCATCTCTGAATTGATAGGGCACTTTTTAGCGAGGCGGAAA
AAGAAAGCGTGAAAGCAACT

Product: bifunctional cbiH protein and precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MQRLARRHQVDQLALTPRAANTLEETPQSLVVGPASEVLSRNWQPNGVLLIVGAIGAVTRLIAPLLIGKDKDPAVIVLDA
HGKHVVPLLGGHQAGAEQLAMELAAELGGHAVLTGDANSQNRLALDSFGKGWGWRRSGSRENWNQLMLTQAQGGKLLLLQ
HSGATFWQTTAAAQDVFDANANADQARPAELTIGPQSSMSCSWHPASLWIGIGCERDTSLSLLERAVAAALAEAGLAPEA
VAGLASIDRKADETALLALAQAKDWPVRFFNADALAEVDVPTPSAVVAKAMGTSSVAEAAALLAASNKSTLLLTKQIHHA
QNAEHGATTIAISEANQPFAPQRGELHLIGSGPGDLAFLTHDARAALARSAIWVGYDLYLDLLEPLRRPDQARLDGQLTR
ERDRCLKALELAQQGARVALISSGDSGIYGMAGLALELWLTQPPSDRPQFQVHPGLSALQLAAAKVGAPLMHDFCSVSLS
DRLTPWSKIETRLKSAASGDFVVALYNPRSQERDWQLTRAIELLLEHRAPSTPVVVARQLGRAQEDIQLHTLQTLPVKEI
DMLTIVLIGNSSSRLQDNHVVTPRGYPGAELA

Sequences:

>Translated_592_residues
MQRLARRHQVDQLALTPRAANTLEETPQSLVVGPASEVLSRNWQPNGVLLIVGAIGAVTRLIAPLLIGKDKDPAVIVLDA
HGKHVVPLLGGHQAGAEQLAMELAAELGGHAVLTGDANSQNRLALDSFGKGWGWRRSGSRENWNQLMLTQAQGGKLLLLQ
HSGATFWQTTAAAQDVFDANANADQARPAELTIGPQSSMSCSWHPASLWIGIGCERDTSLSLLERAVAAALAEAGLAPEA
VAGLASIDRKADETALLALAQAKDWPVRFFNADALAEVDVPTPSAVVAKAMGTSSVAEAAALLAASNKSTLLLTKQIHHA
QNAEHGATTIAISEANQPFAPQRGELHLIGSGPGDLAFLTHDARAALARSAIWVGYDLYLDLLEPLRRPDQARLDGQLTR
ERDRCLKALELAQQGARVALISSGDSGIYGMAGLALELWLTQPPSDRPQFQVHPGLSALQLAAAKVGAPLMHDFCSVSLS
DRLTPWSKIETRLKSAASGDFVVALYNPRSQERDWQLTRAIELLLEHRAPSTPVVVARQLGRAQEDIQLHTLQTLPVKEI
DMLTIVLIGNSSSRLQDNHVVTPRGYPGAELA
>Mature_592_residues
MQRLARRHQVDQLALTPRAANTLEETPQSLVVGPASEVLSRNWQPNGVLLIVGAIGAVTRLIAPLLIGKDKDPAVIVLDA
HGKHVVPLLGGHQAGAEQLAMELAAELGGHAVLTGDANSQNRLALDSFGKGWGWRRSGSRENWNQLMLTQAQGGKLLLLQ
HSGATFWQTTAAAQDVFDANANADQARPAELTIGPQSSMSCSWHPASLWIGIGCERDTSLSLLERAVAAALAEAGLAPEA
VAGLASIDRKADETALLALAQAKDWPVRFFNADALAEVDVPTPSAVVAKAMGTSSVAEAAALLAASNKSTLLLTKQIHHA
QNAEHGATTIAISEANQPFAPQRGELHLIGSGPGDLAFLTHDARAALARSAIWVGYDLYLDLLEPLRRPDQARLDGQLTR
ERDRCLKALELAQQGARVALISSGDSGIYGMAGLALELWLTQPPSDRPQFQVHPGLSALQLAAAKVGAPLMHDFCSVSLS
DRLTPWSKIETRLKSAASGDFVVALYNPRSQERDWQLTRAIELLLEHRAPSTPVVVARQLGRAQEDIQLHTLQTLPVKEI
DMLTIVLIGNSSSRLQDNHVVTPRGYPGAELA

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 63244; Mature: 63244

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLARRHQVDQLALTPRAANTLEETPQSLVVGPASEVLSRNWQPNGVLLIVGAIGAVTR
CCHHHHHHCCHHEEECCCHHHHHHHCCHHEEECCHHHHHHCCCCCCCEEEEEEHHHHHHH
LIAPLLIGKDKDPAVIVLDAHGKHVVPLLGGHQAGAEQLAMELAAELGGHAVLTGDANSQ
HHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
NRLALDSFGKGWGWRRSGSRENWNQLMLTQAQGGKLLLLQHSGATFWQTTAAAQDVFDAN
CCEEHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEHHHHHHHHHCCC
ANADQARPAELTIGPQSSMSCSWHPASLWIGIGCERDTSLSLLERAVAAALAEAGLAPEA
CCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
VAGLASIDRKADETALLALAQAKDWPVRFFNADALAEVDVPTPSAVVAKAMGTSSVAEAA
HHHHHHHHCCCCHHHHHEECCCCCCCEEEECCCCEEEECCCCCHHHHHHHCCCHHHHHHH
ALLAASNKSTLLLTKQIHHAQNAEHGATTIAISEANQPFAPQRGELHLIGSGPGDLAFLT
HHHHCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEE
HDARAALARSAIWVGYDLYLDLLEPLRRPDQARLDGQLTRERDRCLKALELAQQGARVAL
CHHHHHHHHHHHEEHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEE
ISSGDSGIYGMAGLALELWLTQPPSDRPQFQVHPGLSALQLAAAKVGAPLMHDFCSVSLS
EECCCCCCEEHHCEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHCCCHH
DRLTPWSKIETRLKSAASGDFVVALYNPRSQERDWQLTRAIELLLEHRAPSTPVVVARQL
HCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
GRAQEDIQLHTLQTLPVKEIDMLTIVLIGNSSSRLQDNHVVTPRGYPGAELA
CCCHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MQRLARRHQVDQLALTPRAANTLEETPQSLVVGPASEVLSRNWQPNGVLLIVGAIGAVTR
CCHHHHHHCCHHEEECCCHHHHHHHCCHHEEECCHHHHHHCCCCCCCEEEEEEHHHHHHH
LIAPLLIGKDKDPAVIVLDAHGKHVVPLLGGHQAGAEQLAMELAAELGGHAVLTGDANSQ
HHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
NRLALDSFGKGWGWRRSGSRENWNQLMLTQAQGGKLLLLQHSGATFWQTTAAAQDVFDAN
CCEEHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEHHHHHHHHHCCC
ANADQARPAELTIGPQSSMSCSWHPASLWIGIGCERDTSLSLLERAVAAALAEAGLAPEA
CCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
VAGLASIDRKADETALLALAQAKDWPVRFFNADALAEVDVPTPSAVVAKAMGTSSVAEAA
HHHHHHHHCCCCHHHHHEECCCCCCCEEEECCCCEEEECCCCCHHHHHHHCCCHHHHHHH
ALLAASNKSTLLLTKQIHHAQNAEHGATTIAISEANQPFAPQRGELHLIGSGPGDLAFLT
HHHHCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEE
HDARAALARSAIWVGYDLYLDLLEPLRRPDQARLDGQLTRERDRCLKALELAQQGARVAL
CHHHHHHHHHHHEEHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEE
ISSGDSGIYGMAGLALELWLTQPPSDRPQFQVHPGLSALQLAAAKVGAPLMHDFCSVSLS
EECCCCCCEEHHCEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHCCCHH
DRLTPWSKIETRLKSAASGDFVVALYNPRSQERDWQLTRAIELLLEHRAPSTPVVVARQL
HCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
GRAQEDIQLHTLQTLPVKEIDMLTIVLIGNSSSRLQDNHVVTPRGYPGAELA
CCCHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]