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The map label for this gene is truA [H]

Identifier: 33864024

GI number: 33864024

Start: 1848915

End: 1849802

Strand: Direct

Name: truA [H]

Synonym: PMT1757

Alternate gene names: 33864024

Gene position: 1848915-1849802 (Clockwise)

Preceding gene: 33864023

Following gene: 33864025

Centisome position: 76.69

GC content: 54.05

Gene sequence:

>888_bases
ATGTTCCCTAACTCCAAAGTCGAACTGCCAAAAAGTACGCCAGTTCGACGTATTGGCCTCAGCGTGCAATATGAAGGCTC
AAACTTTTGCGGTTGGCAGCGTCAACCACAAGCGGTGAGCGTTCAACAAGTGCTTGAAGATGCAATCTCCCAGCTTGATC
CTCAGAGGCCTATTCAAGCCGTTGCAGCAGGTCGCACAGACGCTGGCGTCCATGCTGCGGGCCAAGTTGTGCACTTCGAC
TGCTGTGGGCCCATCCCAGCCGAACGCTGGGCACCTGCTCTAAACGGACGACTACCTAGCACGATTCGTGTGCGGGAAGC
AGTAGCGCGCCCAGCCTCCTGGCATGCATGTCATTCAGCCATATACCGAAGATATCGCTACACGATCTACAACGGTCGGC
GACCCAATCTGTTCCTCACCCCCTGGAGCTGGCACCGCTACCAAGTTCGTCTTGATGAAACCATGATGCAAATAGCTCTA
GAGGGGCTCCTTGGGTTGCAAGACTTCAGCGCTTTTCAACGAACTGGTAGTCGACGAGCCCATGCCCGCACCACAATTCA
GGAAGTAAAGATTGAGCGGCAGGGAGATTTAATAATGATTGAGATCCAAGCCAGCGGCTTCCTCTACGGCATGGTGCGGC
TGTTGATGGGCCAATTGGTGGCCTTAGGCGAACACCGACTTAATCTTGAGACCTTTGAGCGGCGCTGGAAGGAGAAACGC
CGTCAAGAAGTGAAAGAAGCGGCACCAGCTCAGGGTCTTTGCCTCCTGCGTGCAGGATACGAAGAAGACATTTTTTCTAA
AGGCGGTTGGTATGACTGCCAACCGCGCTATTCCCTCGGAGCAAATGATCCTCCTTTGGATCCTCCATCTGCACCGGAGT
CAAGCTGA

Upstream 100 bases:

>100_bases
TCATCCGTACGGTGCCTCGCCGCGGCGACAATGCTGAGATGGCAATCATAGAGTTGGTCTAAAGCCTGGGAACAGTGACA
ACATGCTGAACTGATCAGCA

Downstream 100 bases:

>100_bases
ACTCCCACTACAACGCCTCACCCCTAAGGCAAACACAAGGGCTTCGCGAACCATTCCACGAGTGATCTTTTTGGATTTGC
TCCCATGAAAGGCTAAAGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEDAISQLDPQRPIQAVAAGRTDAGVHAAGQVVHFD
CCGPIPAERWAPALNGRLPSTIRVREAVARPASWHACHSAIYRRYRYTIYNGRRPNLFLTPWSWHRYQVRLDETMMQIAL
EGLLGLQDFSAFQRTGSRRAHARTTIQEVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLETFERRWKEKR
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS

Sequences:

>Translated_295_residues
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEDAISQLDPQRPIQAVAAGRTDAGVHAAGQVVHFD
CCGPIPAERWAPALNGRLPSTIRVREAVARPASWHACHSAIYRRYRYTIYNGRRPNLFLTPWSWHRYQVRLDETMMQIAL
EGLLGLQDFSAFQRTGSRRAHARTTIQEVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLETFERRWKEKR
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS
>Mature_295_residues
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEDAISQLDPQRPIQAVAAGRTDAGVHAAGQVVHFD
CCGPIPAERWAPALNGRLPSTIRVREAVARPASWHACHSAIYRRYRYTIYNGRRPNLFLTPWSWHRYQVRLDETMMQIAL
EGLLGLQDFSAFQRTGSRRAHARTTIQEVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLETFERRWKEKR
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=280, Percent_Identity=33.9285714285714, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=36.5461847389558, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 33414; Mature: 33414

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEDAISQLDPQRPIQA
CCCCCCEECCCCCCHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
VAAGRTDAGVHAAGQVVHFDCCGPIPAERWAPALNGRLPSTIRVREAVARPASWHACHSA
HHCCCCCCCCCCCCCEEEEECCCCCCHHHCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHH
IYRRYRYTIYNGRRPNLFLTPWSWHRYQVRLDETMMQIALEGLLGLQDFSAFQRTGSRRA
HHHHHHEEEECCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCHH
HARTTIQEVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLETFERRWKEKR
HHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS
HHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPNSKVELPKSTPVRRIGLSVQYEGSNFCGWQRQPQAVSVQQVLEDAISQLDPQRPIQA
CCCCCCEECCCCCCHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
VAAGRTDAGVHAAGQVVHFDCCGPIPAERWAPALNGRLPSTIRVREAVARPASWHACHSA
HHCCCCCCCCCCCCCEEEEECCCCCCHHHCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHH
IYRRYRYTIYNGRRPNLFLTPWSWHRYQVRLDETMMQIALEGLLGLQDFSAFQRTGSRRA
HHHHHHEEEECCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCHH
HARTTIQEVKIERQGDLIMIEIQASGFLYGMVRLLMGQLVALGEHRLNLETFERRWKEKR
HHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RQEVKEAAPAQGLCLLRAGYEEDIFSKGGWYDCQPRYSLGANDPPLDPPSAPESS
HHHHHHHCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA