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The map label for this gene is rlmN [H]

Identifier: 33863770

GI number: 33863770

Start: 1585854

End: 1586924

Strand: Reverse

Name: rlmN [H]

Synonym: PMT1503

Alternate gene names: 33863770

Gene position: 1586924-1585854 (Counterclockwise)

Preceding gene: 33863771

Following gene: 33863769

Centisome position: 65.82

GC content: 56.3

Gene sequence:

>1071_bases
GTGAGTTCTACCCATCTGCCTGGCGGCAATCAGGCGTTACTGGGTTGTAGTGCTACTGAACTAGAGGGCTGGGCTGTGGC
AGAAGGCCAGCCAGCCTTCCGGGGCCGTCAGTTGCATGAATGGCTCTATGCCAAGGGAGCCAGAAGTTTTGAGGCGATCA
CAGTGTTGCCAAAGTCATGGCGCCTCTCGTTGCAACAGCGAGGCATCACGATTGGGCGGTTGTTGGAGGTGAACCGGGCT
GTTGCGGTGGATGACACGACCAAGTTGCTGCTGGCAACGGTTGATGGCGAAACCATTGAGAGTGTAGGCATCCCAACCCA
GCAACGTCTCACAGTGTGCTTATCCAGTCAGGTGGGCTGTCCAATGGCTTGTCGCTTCTGTGCTAGCGGGAAGGGAGGGC
TGCAACGATCGCTGGCCACACATGAGATTGTTGATCAGGTGTTGAGCTTGCGCGAGGCGATGGATCGCCGTCCTTCCCAC
GTTGTCTTCATGGGTATGGGTGAGCCTTTGCTCAACATTGAAGCGGTCTTGGCGGCGATTCGTTGCCTGAATATTGATCT
AGGTATTGCTCAGCGTCGTATCACTGTGAGCACCGTGGGTGTCCCCCATACGCTGCCCCATCTGGCAGAGCTGGCGATGA
AACGTCTAGGTCGCGCCCAGTTCACCCTGGCCGTGAGTCTGCATGCCCCCAATCAAGAGTTGCGTGAACGTTTGATACCT
ACCGCTTGCGCTTACCCCTTCGAGACTCTGCTTCAAGACTGTCGCCATTATTTGGCAGTGACTGGTCGACGGGTGACTTT
TGAGTACATCCTGCTTGGAGCACTTAATGATCAGCCTCAGCATGCAGAAGAACTAGCGGATCGGGTTAGGGGCTTCCAGA
GTCATGTGAATCTGATTGCCTACAACCCCATCGACGATGAGGGTTTTCAACGTCCTAATCCAGAGACGATTGAGGCCTTT
CGGCGTGTATTGGAGCAGCGTGGGGTGGCAGTGAGTTTGCGTGCAAGTCGAGGGCTGGATCAGAATGCCGCCTGTGGTCA
GCTCCGCCGCCAGCACGCTGCCACCGGCTGA

Upstream 100 bases:

>100_bases
TTCACAGCCATAACGAGAAGCTCAATGGACGTATGGCGATGCTTGGCTTTATTGCCTTAATGGTGGTGGAGGCAACACTC
GGGCATGGTTTGTTGATCTG

Downstream 100 bases:

>100_bases
GATGGACTAGCTGAGTCACACTGCGATCAACCGCCTAAGGATATGGCTGCCATTGATTGGATGATCCTCGTTGGTTATCT
CGCCATCACTCTCGTGATTG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSSTHLPGGNQALLGCSATELEGWAVAEGQPAFRGRQLHEWLYAKGARSFEAITVLPKSWRLSLQQRGITIGRLLEVNRA
VAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGCPMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSH
VVFMGMGEPLLNIEAVLAAIRCLNIDLGIAQRRITVSTVGVPHTLPHLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELADRVRGFQSHVNLIAYNPIDDEGFQRPNPETIEAF
RRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAATG

Sequences:

>Translated_356_residues
MSSTHLPGGNQALLGCSATELEGWAVAEGQPAFRGRQLHEWLYAKGARSFEAITVLPKSWRLSLQQRGITIGRLLEVNRA
VAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGCPMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSH
VVFMGMGEPLLNIEAVLAAIRCLNIDLGIAQRRITVSTVGVPHTLPHLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELADRVRGFQSHVNLIAYNPIDDEGFQRPNPETIEAF
RRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAATG
>Mature_355_residues
SSTHLPGGNQALLGCSATELEGWAVAEGQPAFRGRQLHEWLYAKGARSFEAITVLPKSWRLSLQQRGITIGRLLEVNRAV
AVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGCPMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSHV
VFMGMGEPLLNIEAVLAAIRCLNIDLGIAQRRITVSTVGVPHTLPHLAELAMKRLGRAQFTLAVSLHAPNQELRERLIPT
ACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELADRVRGFQSHVNLIAYNPIDDEGFQRPNPETIEAFR
RVLEQRGVAVSLRASRGLDQNAACGQLRRQHAATG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=353, Percent_Identity=34.2776203966006, Blast_Score=201, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39119; Mature: 38987

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTHLPGGNQALLGCSATELEGWAVAEGQPAFRGRQLHEWLYAKGARSFEAITVLPKSW
CCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCHHH
RLSLQQRGITIGRLLEVNRAVAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGC
HHHHHHCCCHHHHHHHHCCEEEECCCCEEEEEEECCCHHHHCCCCCHHHHHHHHCCCCCC
PMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSHVVFMGMGEPLLNIEAVLAAI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
RCLNIDLGIAQRRITVSTVGVPHTLPHLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHCC
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELADRVRGFQSHVNLIA
HHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEE
YNPIDDEGFQRPNPETIEAFRRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAATG
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSTHLPGGNQALLGCSATELEGWAVAEGQPAFRGRQLHEWLYAKGARSFEAITVLPKSW
CCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCHHH
RLSLQQRGITIGRLLEVNRAVAVDDTTKLLLATVDGETIESVGIPTQQRLTVCLSSQVGC
HHHHHHCCCHHHHHHHHCCEEEECCCCEEEEEEECCCHHHHCCCCCHHHHHHHHCCCCCC
PMACRFCASGKGGLQRSLATHEIVDQVLSLREAMDRRPSHVVFMGMGEPLLNIEAVLAAI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
RCLNIDLGIAQRRITVSTVGVPHTLPHLAELAMKRLGRAQFTLAVSLHAPNQELRERLIP
HHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHCC
TACAYPFETLLQDCRHYLAVTGRRVTFEYILLGALNDQPQHAEELADRVRGFQSHVNLIA
HHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEE
YNPIDDEGFQRPNPETIEAFRRVLEQRGVAVSLRASRGLDQNAACGQLRRQHAATG
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA