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The map label for this gene is murD

Identifier: 33863701

GI number: 33863701

Start: 1522255

End: 1523637

Strand: Direct

Name: murD

Synonym: PMT1434

Alternate gene names: 33863701

Gene position: 1522255-1523637 (Clockwise)

Preceding gene: 33863700

Following gene: 33863706

Centisome position: 63.14

GC content: 57.19

Gene sequence:

>1383_bases
ATGGCACGCACTGTGGTCGTGGGGCTTGGACGCTCCGGCATCGGTGCGGCACGACTTCTGCAAGCAGAGGGACATCAAGT
CACAGTGCTTGAGCGCAGCATCGAGCCCCATCTCCAAAGCTTGGCAGCTGATCTACGTCTGCAGGGAATTGCTGTCGAGC
TAGGCAAACCTCTAGAGCTGAACAGCTTCATCCCTTTGCTTGATCAACTTGATGCCGTGGTCATCAGCCCTGGCATCGCC
TGGGACCACCCAACACTGACTGCACTGCGTCAACGCGGCATCGACATCGATGGAGAGATGGCAGTGGCCTGGCGAAGCCT
GAGCCATCTGCCATGGATCGCCATTACAGGCACGAATGGCAAAACAACAGTGACCCACCTGCTCAATCATGTGCTTGAAA
GCAATGGCCTCAGAGCACCAATGGGCGGCAATGTTGGCCATGCCGCGGCTGAAGTCGCACTCAAATGGCGGCAACCAAAC
GCACAGCGCCCCGACTGGCTGGTCATGGAGCTCAGCAGTTACCAGATCGAAGCCGCCCCTGAGATTGCCCCACGCATGGG
CATTTGGACCAACCTCACCCCCGACCACCTCGAACGCCACGGCACCCTTGATGCTTACCGAACGATCAAGCGTGGCCTAC
TGGAGCGCTCAGAGATCCGCATCTTCAATGGCGATGATCCAGATCTTCGCAGCCAAAGATCCAGCTGGGATAAGGGGCTG
TGGGTGAGCAGCGAAGGGCCTGGTACTGCAAACCATCGCGCCGACTTTTGGATTGACGCTGAGGGGCTGGTTAGGGAACC
GCAAGGAGCGCTTTTTGCCGCTTCGGCTTTGGCCATGCCTGGACAACACAATCTTCAAAACCTGCTGCTAGTGACAGCCG
CAGCCCGGCAAACGGGTTTGCCAGCAAAGGCCATCGAAGCCAGCCTGCGCTCGTTCCCAGGCGTGCCTCACAGACTCGAA
CAGCTTGGGCATATCCAGCAAATGAGCGTCTACAACGACAGCAAGGCCACGAACTATGACGCCGCCTGTGTGGGCCTCAA
GGCCGTCCCTCCTCCTGCCGTTGTGCTTGCAGGTGGGCAAACCAAACAGGGCGATGCTTCTGGATGGCTCAAGCAATTGG
ATCAGAACGCCTGCGCTGTAATCCTGTTTGGTGCAGGCGCTTCAGAACTGCAAGAGCTGATCAAAACGTCTGGCTTCAGT
GGTGAGTTGCATTGCTGCAGCAACCTCAACGCTGCTGTGACTTTGGCCATTCCACTGGGCGTAGAGCGACAAGCCGCTTG
CCTGTTGCTTTCACCTGCCTGCGCAAGTTTTGATCAATACCAAGACTTCGAAGCCCGAGGCGAACATTTCCGTAGCCTGA
TTTCATCACATTTAACGGCCTAA

Upstream 100 bases:

>100_bases
CGGGCGATTAGCTCAGAGGTAGAGCACTACCTTGACACGGTAGGAGTCACTGGTTCGATTCCAGTATCGCCCACTCCCCT
TACAAACACAGGACTCATCG

Downstream 100 bases:

>100_bases
TCTACGGCTAACTGATCACTAGCTTCAACTTCATGCTCATTCCTTTGTTCGATAATGATGCGACTGGGGCTCTATTTATA
ACGTCAATCCTACTAGTTTA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MARTVVVGLGRSGIGAARLLQAEGHQVTVLERSIEPHLQSLAADLRLQGIAVELGKPLELNSFIPLLDQLDAVVISPGIA
WDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNGKTTVTHLLNHVLESNGLRAPMGGNVGHAAAEVALKWRQPN
AQRPDWLVMELSSYQIEAAPEIAPRMGIWTNLTPDHLERHGTLDAYRTIKRGLLERSEIRIFNGDDPDLRSQRSSWDKGL
WVSSEGPGTANHRADFWIDAEGLVREPQGALFAASALAMPGQHNLQNLLLVTAAARQTGLPAKAIEASLRSFPGVPHRLE
QLGHIQQMSVYNDSKATNYDAACVGLKAVPPPAVVLAGGQTKQGDASGWLKQLDQNACAVILFGAGASELQELIKTSGFS
GELHCCSNLNAAVTLAIPLGVERQAACLLLSPACASFDQYQDFEARGEHFRSLISSHLTA

Sequences:

>Translated_460_residues
MARTVVVGLGRSGIGAARLLQAEGHQVTVLERSIEPHLQSLAADLRLQGIAVELGKPLELNSFIPLLDQLDAVVISPGIA
WDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNGKTTVTHLLNHVLESNGLRAPMGGNVGHAAAEVALKWRQPN
AQRPDWLVMELSSYQIEAAPEIAPRMGIWTNLTPDHLERHGTLDAYRTIKRGLLERSEIRIFNGDDPDLRSQRSSWDKGL
WVSSEGPGTANHRADFWIDAEGLVREPQGALFAASALAMPGQHNLQNLLLVTAAARQTGLPAKAIEASLRSFPGVPHRLE
QLGHIQQMSVYNDSKATNYDAACVGLKAVPPPAVVLAGGQTKQGDASGWLKQLDQNACAVILFGAGASELQELIKTSGFS
GELHCCSNLNAAVTLAIPLGVERQAACLLLSPACASFDQYQDFEARGEHFRSLISSHLTA
>Mature_459_residues
ARTVVVGLGRSGIGAARLLQAEGHQVTVLERSIEPHLQSLAADLRLQGIAVELGKPLELNSFIPLLDQLDAVVISPGIAW
DHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNGKTTVTHLLNHVLESNGLRAPMGGNVGHAAAEVALKWRQPNA
QRPDWLVMELSSYQIEAAPEIAPRMGIWTNLTPDHLERHGTLDAYRTIKRGLLERSEIRIFNGDDPDLRSQRSSWDKGLW
VSSEGPGTANHRADFWIDAEGLVREPQGALFAASALAMPGQHNLQNLLLVTAAARQTGLPAKAIEASLRSFPGVPHRLEQ
LGHIQQMSVYNDSKATNYDAACVGLKAVPPPAVVLAGGQTKQGDASGWLKQLDQNACAVILFGAGASELQELIKTSGFSG
ELHCCSNLNAAVTLAIPLGVERQAACLLLSPACASFDQYQDFEARGEHFRSLISSHLTA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=29.9781181619256, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PROMM (Q7V5V5)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895261.1
- ProteinModelPortal:   Q7V5V5
- SMR:   Q7V5V5
- STRING:   Q7V5V5
- GeneID:   1729414
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1434
- NMPDR:   fig|74547.1.peg.1428
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02705
- BioCyc:   PMAR74547:PMT1434-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49544; Mature: 49413

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTVVVGLGRSGIGAARLLQAEGHQVTVLERSIEPHLQSLAADLRLQGIAVELGKPLEL
CCCEEEEECCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHEEEEEEEECCCCCCC
NSFIPLLDQLDAVVISPGIAWDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNG
CHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCC
KTTVTHLLNHVLESNGLRAPMGGNVGHAAAEVALKWRQPNAQRPDWLVMELSSYQIEAAP
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEEEECCCEEECCC
EIAPRMGIWTNLTPDHLERHGTLDAYRTIKRGLLERSEIRIFNGDDPDLRSQRSSWDKGL
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCE
WVSSEGPGTANHRADFWIDAEGLVREPQGALFAASALAMPGQHNLQNLLLVTAAARQTGL
EEECCCCCCCCCCCEEEECCCCCCCCCCCCEEEHHHHCCCCCCCHHHHHEEEEHHHHCCC
PAKAIEASLRSFPGVPHRLEQLGHIQQMSVYNDSKATNYDAACVGLKAVPPPAVVLAGGQ
CHHHHHHHHHHCCCCCHHHHHHCCHHHHHEECCCCCCCCCHHEEECCCCCCCEEEEECCC
TKQGDASGWLKQLDQNACAVILFGAGASELQELIKTSGFSGELHCCSNLNAAVTLAIPLG
CCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECC
VERQAACLLLSPACASFDQYQDFEARGEHFRSLISSHLTA
CCCCCEEEEECHHHHCHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARTVVVGLGRSGIGAARLLQAEGHQVTVLERSIEPHLQSLAADLRLQGIAVELGKPLEL
CCEEEEECCCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHEEEEEEEECCCCCCC
NSFIPLLDQLDAVVISPGIAWDHPTLTALRQRGIDIDGEMAVAWRSLSHLPWIAITGTNG
CHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCC
KTTVTHLLNHVLESNGLRAPMGGNVGHAAAEVALKWRQPNAQRPDWLVMELSSYQIEAAP
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEEEECCCEEECCC
EIAPRMGIWTNLTPDHLERHGTLDAYRTIKRGLLERSEIRIFNGDDPDLRSQRSSWDKGL
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCE
WVSSEGPGTANHRADFWIDAEGLVREPQGALFAASALAMPGQHNLQNLLLVTAAARQTGL
EEECCCCCCCCCCCEEEECCCCCCCCCCCCEEEHHHHCCCCCCCHHHHHEEEEHHHHCCC
PAKAIEASLRSFPGVPHRLEQLGHIQQMSVYNDSKATNYDAACVGLKAVPPPAVVLAGGQ
CHHHHHHHHHHCCCCCHHHHHHCCHHHHHEECCCCCCCCCHHEEECCCCCCCEEEEECCC
TKQGDASGWLKQLDQNACAVILFGAGASELQELIKTSGFSGELHCCSNLNAAVTLAIPLG
CCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECC
VERQAACLLLSPACASFDQYQDFEARGEHFRSLISSHLTA
CCCCCEEEEECHHHHCHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642