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The map label for this gene is truB

Identifier: 33863689

GI number: 33863689

Start: 1509744

End: 1510706

Strand: Direct

Name: truB

Synonym: PMT1422

Alternate gene names: 33863689

Gene position: 1509744-1510706 (Clockwise)

Preceding gene: 33863688

Following gene: 33863690

Centisome position: 62.62

GC content: 57.53

Gene sequence:

>963_bases
GTGACTGTGCCCTTCGGCTTTGTGGTGATCGACAAGCCTGCTGGCATCACCTCCCACGACTGTGTGAGCCGAATGCGGCG
CGTGTTTGGCATCAAGCGCGTGGGCCACGGCGGCACGCTTGATCCTGCCGTAACAGGAGTTTTGCCCATCGCCCTAGGGC
ATGCCACCAGGCTGCTGCCCTATCTACCCGGCGCCAAGAGCTATCGCGGCAGCATCCAACTGGGCCAACGCACCAGTAGC
GACGATCAACAAGGGGATCTGATCTCCAAGCAAGCCTGGCCCGAATTGAACACAGCAGAGATCGAGGCCTATCTCGAGCC
GTTTCGCGGCAGGATTCAGCAACGGCCACCGCAAGTTTCAGCCGTGCATGTGCAAGGGGAACGCGCCCATGCACGTGCCC
GCAGGGGAGAAACGATGGAGATTCCAGCGCGAACGATCACGATTGATCGCTTGCAGCTACTCAACTGGAACCAACAACTG
GGCCAAATCGACTTCAACGTGCATTGCTCATCAGGCACATACATTCGCTCTCTCGCACGAGATCTAGGCGAATTAATTGG
CTGCGGCGCTTGTCTGGGGTGGTTAAAGCGAACCCAGGCACTGGGGTTTCACGAGCAACAAGCCGTACCACTACCCGATC
GAGACAATCCAGCCCTAACGACTCCGCCTGCTGTGTTACCGCCACTCACGGCCCTTGCACACCTACCAAGACTGCAACTC
AACGAAGAGGAACAGGAGAGCTGGAGCTGCGGGCGCCGCATTACCGCCCATCAAGATCAATGCCAACCAGCACCCAAACC
TCTGGCCTCTGATCAGCAAGAATCTGCTCCAAACCAAACTGATCCAAGCGCAAACAAGTCCATGCTCGTGGTGATCGACT
GCAGAGGCGAAGTCGCCGGAATGGCCTACTGGGAAGACAACGCAACAGTGAAGCCAAAAGTTGTGTTCAACGCACAGGGA
TGA

Upstream 100 bases:

>100_bases
CAACCGCAAGCGCATCAGTGTGGTTGCCTCCAGCTGAGCAATCTCAGCCTTTGCAGACAATGCGGGCAGCAGCAGTCACA
AACTGAACGCTGCTGATCAG

Downstream 100 bases:

>100_bases
TCGCAACACTGTCGTTCAACGCGAACGCCCCTAGCGTTGATGGTCAAGCAACAACCTAATCAGCCTCCGCCATGGCCGGC
CATAGCAAATGGTCTCAAAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTVPFGFVVIDKPAGITSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYLPGAKSYRGSIQLGQRTSS
DDQQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAVHVQGERAHARARRGETMEIPARTITIDRLQLLNWNQQL
GQIDFNVHCSSGTYIRSLARDLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPALTTPPAVLPPLTALAHLPRLQL
NEEEQESWSCGRRITAHQDQCQPAPKPLASDQQESAPNQTDPSANKSMLVVIDCRGEVAGMAYWEDNATVKPKVVFNAQG

Sequences:

>Translated_320_residues
MTVPFGFVVIDKPAGITSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYLPGAKSYRGSIQLGQRTSS
DDQQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAVHVQGERAHARARRGETMEIPARTITIDRLQLLNWNQQL
GQIDFNVHCSSGTYIRSLARDLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPALTTPPAVLPPLTALAHLPRLQL
NEEEQESWSCGRRITAHQDQCQPAPKPLASDQQESAPNQTDPSANKSMLVVIDCRGEVAGMAYWEDNATVKPKVVFNAQG
>Mature_319_residues
TVPFGFVVIDKPAGITSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLPYLPGAKSYRGSIQLGQRTSSD
DQQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVSAVHVQGERAHARARRGETMEIPARTITIDRLQLLNWNQQLG
QIDFNVHCSSGTYIRSLARDLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPALTTPPAVLPPLTALAHLPRLQLN
EEEQESWSCGRRITAHQDQCQPAPKPLASDQQESAPNQTDPSANKSMLVVIDCRGEVAGMAYWEDNATVKPKVVFNAQG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=224, Percent_Identity=32.1428571428571, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI215599015, Length=174, Percent_Identity=31.6091954022989, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4503337, Length=174, Percent_Identity=31.6091954022989, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367200, Length=209, Percent_Identity=37.799043062201, Blast_Score=148, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553978, Length=199, Percent_Identity=31.6582914572864, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=205, Percent_Identity=29.7560975609756, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=136, Percent_Identity=35.2941176470588, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=29.5918367346939, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=29.5918367346939, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=29.5918367346939, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=29.5918367346939, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=29.5918367346939, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=29.5918367346939, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PROMM (P59881)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_895249.1
- ProteinModelPortal:   P59881
- SMR:   P59881
- STRING:   P59881
- GeneID:   1728218
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1422
- NMPDR:   fig|74547.1.peg.1416
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   IPARTIT
- ProtClustDB:   PRK02755
- BioCyc:   PMAR74547:PMT1422-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 35093; Mature: 34962

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: ACT_SITE 41-41

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPFGFVVIDKPAGITSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLP
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YLPGAKSYRGSIQLGQRTSSDDQQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVS
CCCCCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
AVHVQGERAHARARRGETMEIPARTITIDRLQLLNWNQQLGQIDFNVHCSSGTYIRSLAR
EEEEECHHHHHHHHCCCEEECCCEEEEEHHHHHHCCCHHHCCEEEEEEECCCHHHHHHHH
DLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPALTTPPAVLPPLTALAHLPRLQL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
NEEEQESWSCGRRITAHQDQCQPAPKPLASDQQESAPNQTDPSANKSMLVVIDCRGEVAG
CCHHHHHHHHCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCEEE
MAYWEDNATVKPKVVFNAQG
EEEECCCCEECCEEEEECCC
>Mature Secondary Structure 
TVPFGFVVIDKPAGITSHDCVSRMRRVFGIKRVGHGGTLDPAVTGVLPIALGHATRLLP
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YLPGAKSYRGSIQLGQRTSSDDQQGDLISKQAWPELNTAEIEAYLEPFRGRIQQRPPQVS
CCCCCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
AVHVQGERAHARARRGETMEIPARTITIDRLQLLNWNQQLGQIDFNVHCSSGTYIRSLAR
EEEEECHHHHHHHHCCCEEECCCEEEEEHHHHHHCCCHHHCCEEEEEEECCCHHHHHHHH
DLGELIGCGACLGWLKRTQALGFHEQQAVPLPDRDNPALTTPPAVLPPLTALAHLPRLQL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
NEEEQESWSCGRRITAHQDQCQPAPKPLASDQQESAPNQTDPSANKSMLVVIDCRGEVAG
CCHHHHHHHHCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCEEE
MAYWEDNATVKPKVVFNAQG
EEEECCCCEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642