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The map label for this gene is ndhB [H]

Identifier: 33863621

GI number: 33863621

Start: 1439686

End: 1441257

Strand: Direct

Name: ndhB [H]

Synonym: PMT1354

Alternate gene names: 33863621

Gene position: 1439686-1441257 (Clockwise)

Preceding gene: 33863615

Following gene: 33863622

Centisome position: 59.72

GC content: 54.96

Gene sequence:

>1572_bases
ATGCCCGACATGGGTGTATTTCTTCTCGCCACCCAGGCCGTGGCACCTCCAGGGGAGCTGCTCAACCTTGCACTCAATGC
GGGTGCGATTGCCCCTGAAGGGGCTGTTCTCGTCGCAATGCTGGCGACCCTACTTGTGGACTTGGCCGGCGAGCAGGCTG
CAGCCCGCTGGGTACCCCCCATTTGTTATGCCGGCCTTGGCACAGCACTCGTGCTCCTCGCCCAGCAATGGAATGCCCCT
CTGGAACCCTCGTTCCTTGGAGCTTTTCTGGCTGACAACCTGGCCATTTCCTTTCGGGCCGTGGTGGCGCTCTCGACATT
GTTGTCACTACTGATCAGCTGGCGCTACGCGGAACAGAGCGGCACGCCAATTGGTGAATATGCCGCCATCCTTCTGGCCG
CAACCCTCGGGGCAATGCTCCTTTGCGGCTCAACTGATCTTGTGAGCGTGTTCGTCTCCCTCGAGACCCTCTCCGTGGCG
AGCTACCTACTGGCTGGCTACATGAAACGAGATGCCCGCAGTTCCGAAGCAGCACTCAAATATTTGCTGGTGGGCTCCGC
CGCCGCCGCAGTTTTTCTCTACGGCGCCTCTCTTCTCTACGGGCTCAGCGGTACCACCAGCTTGCAGGCTATTGGCCTTG
CTTTACTCACCAGCCCTACTCCACTGGCCGCACTATCACTGGTCTTCGTACTTGCCACGGTGGCCTTCAAAATTGCTGCG
GTGCCTTTCCACCAATGGACCCCGGATGTCTATGAAGGATCGCCAACTCCAGTCGTCGCATTCCTCTCAGTGGGATCTAA
AGCTGCTGGCTTTGCTCTTGCACTGAGGTTGCTGGTTGGTTGCTTCGGTGCCTTCGACAACCAATGGAAATTGCTTTTCA
CCGTGCTTGCTGTGTTGAGCATGACCCTAGGCAACGTCGTCGCGCTGGCGCAGACATCAATGAAGCGAATGCTGGCTTAC
AGCTCCATCGGCCAGGCAGGATTCGTGATGATTGGCCTGGTGTGCGGAACCGAAGACGGCTTCGCTGCAATGGTTCTCTA
TATGGCGGCCTATCTATTCATGAACCTTGGAGCCTTTGCCTGCATCATCCTCTTTTCCATCCGCACGGGAAGTGATCGAA
TTTCTGACTATGCAGGTCTCTATCAGAAGGATCCGTTGATAACGCTGGGACTCAGCCTATGCCTTCTTTCCTTGGGTGGC
ATACCTCCGATGCTCGGATTTTTTGGCAAGATCTACCTTTTCTTTGCTGGCTGGGCCGACCATCAATACCTCCTCGTCGT
TGTTGGCCTAGTGACCTCCGTAGTTTCGATTTATTACTACATCTCTGTCATCAAAATGATGGTGGTCAAAGAACCAAAAG
AAGCGTCCGATGTCGTTAAGTCCTATCCATCGATCAAGTGGTCAACGATTGGCATGCCTCCACTTCGAATAGCACTAGTG
GGTTGCGTTGTTGTAACAGCCGTTGGGGGGATTCTTTCAAATCCTCTCTTTCAATGGGCTAATAATGCCGTAGCCGGCAC
GCCGCTTCTCCAGGAAGCCATTGCTCTAGGCAGCCAAAGATCCATTGGCTAA

Upstream 100 bases:

>100_bases
ATCGCACCGACAAGCGAGCTGCATGATGCGATGTTCTCAATAAGGTATAGCAAGGCAGCACAGCTACAGTCACGCAAATT
GGTCTGATCGTGCACGCGTC

Downstream 100 bases:

>100_bases
TCCAACGGCAAGCAGCACACCCGTCGCCGTGCTGAGCGATGTTAGCAAGCTTTACGGACAAGGAGCTGGAACAGTAAAAG
CTCTAGACCATCTCAATCTG

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2 [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPDMGVFLLATQAVAPPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPPICYAGLGTALVLLAQQWNAP
LEPSFLGAFLADNLAISFRAVVALSTLLSLLISWRYAEQSGTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVA
SYLLAGYMKRDARSSEAALKYLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGLALLTSPTPLAALSLVFVLATVAFKIAA
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLSMTLGNVVALAQTSMKRMLAY
SSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFACIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGG
IPPMLGFFGKIYLFFAGWADHQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIKWSTIGMPPLRIALV
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG

Sequences:

>Translated_523_residues
MPDMGVFLLATQAVAPPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPPICYAGLGTALVLLAQQWNAP
LEPSFLGAFLADNLAISFRAVVALSTLLSLLISWRYAEQSGTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVA
SYLLAGYMKRDARSSEAALKYLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGLALLTSPTPLAALSLVFVLATVAFKIAA
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLSMTLGNVVALAQTSMKRMLAY
SSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFACIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGG
IPPMLGFFGKIYLFFAGWADHQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIKWSTIGMPPLRIALV
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG
>Mature_522_residues
PDMGVFLLATQAVAPPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPPICYAGLGTALVLLAQQWNAPL
EPSFLGAFLADNLAISFRAVVALSTLLSLLISWRYAEQSGTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVAS
YLLAGYMKRDARSSEAALKYLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGLALLTSPTPLAALSLVFVLATVAFKIAAV
PFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLSMTLGNVVALAQTSMKRMLAYS
SIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFACIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGGI
PPMLGFFGKIYLFFAGWADHQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIKWSTIGMPPLRIALVG
CVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=318, Percent_Identity=25.1572327044025, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI145693160, Length=372, Percent_Identity=34.4086021505376, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788829, Length=354, Percent_Identity=28.2485875706215, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788831, Length=324, Percent_Identity=25.6172839506173, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788613, Length=361, Percent_Identity=26.0387811634349, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788614, Length=385, Percent_Identity=23.8961038961039, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54838; Mature: 54706

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDMGVFLLATQAVAPPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPP
CCCCCEEEEEHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCH
ICYAGLGTALVLLAQQWNAPLEPSFLGAFLADNLAISFRAVVALSTLLSLLISWRYAEQS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVASYLLAGYMKRDARSSEAALK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGLALLTSPTPLAALSLVFVLATVAFKIAA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLS
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MTLGNVVALAQTSMKRMLAYSSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
CIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGGIPPMLGFFGKIYLFFAGWAD
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIKWSTIGMPPLRIALV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG
HHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PDMGVFLLATQAVAPPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPP
CCCCEEEEEHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCH
ICYAGLGTALVLLAQQWNAPLEPSFLGAFLADNLAISFRAVVALSTLLSLLISWRYAEQS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVASYLLAGYMKRDARSSEAALK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGLALLTSPTPLAALSLVFVLATVAFKIAA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLS
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MTLGNVVALAQTSMKRMLAYSSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
CIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGGIPPMLGFFGKIYLFFAGWAD
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIKWSTIGMPPLRIALV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG
HHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA