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The map label for this gene is gltX [H]

Identifier: 33863576

GI number: 33863576

Start: 1395402

End: 1396832

Strand: Direct

Name: gltX [H]

Synonym: PMT1308

Alternate gene names: 33863576

Gene position: 1395402-1396832 (Clockwise)

Preceding gene: 33863575

Following gene: 33863581

Centisome position: 57.88

GC content: 54.09

Gene sequence:

>1431_bases
GTGAAGGTGCGCGTTCGGCTGGCCCCGAGTCCAACAGGCACACTTCACATCGGCACGGCAAGAACGGCAGTTTTCAACTG
GCTCTTTGCACGCCATCAGGGAGGACAATTCCTCTTGCGCATCGAGGACACCGACAAAGAACGATCCAAGCCAGAATTCA
CCACCAACATCCTCGAAGGTCTGAAGTGGCTTGGCCTCAACTGGGATGAACAGCCCATCATTCAAAGCCAGCATGTGGAT
GACCATCGCGCAGCAATCCAAAAGCTGCTCGATCGTGACCTGGCCTACCGCTGCTACGCCAGCGAGACTGAACTGGAGGC
CATGCGAGAAACACAAAAGGCCCAGGGCAAGGCCCCTCGCTACGACAACCGTCACCGAGATCTCAATCCCGAGCAAGAAG
CCGCCTTCCAATCTGAAGGTAGAACTGCCGTGGTGCGCTTCCGTATCGATGATGATGCGACCATCAGTTGGAAGGACCTG
GTGCGCGGTCCAATGCACTGGAAGGGATCAGATTTGGGCGGCGACATGGTGATCTCACGTCGGGCTCCAGCCAAGGAAAT
TGGAGACCCGCTCTACAACCTTGTTGTGGTGGTTGATGACGCCGCCATGAGTATCTCGCATGTGATCCGCGGCGAAGATC
ACATCGCAAACACGGCCAAACAACTGCTGATCTATGAGGCCCTTGGATTACCAATACCTCAATTCGCCCATACCCCGCTC
ATTCTCAATTCCGAAGGACGCAAACTCTCCAAGCGGGATGGAGTGACCTCGATCAGTGACTTCCAAGCCATGGGGTACAC
AGCCGAAGCTATGGCCAACTACATGTCCCTGCTGGGTTGGTCAGTCCCCGAAGGGACTGACGAACGGTTCACTCTTCAGC
AAGCTGCAACCGTCTTCAGTTTTGACCGAGTCAATAAAGCAGGAGCAAAGTTTGACTGGGACAAACTCAACTGGCTCAAC
TCTCAAGTGCTACATGACTTGCCTAAAGATCAACTGTTGCATGAACTCAAACCCTTGTGGTCTAAGGCTGGCTGGGCTCT
ACCAGAAGAAAACTGGTGTTTGGATCTAGCCGAGCTGCTTGGACCATCGCTGACACTGCTCAAGGATGGTGTCGATCAAG
CTCGCCCTTTTTTCGAAGAACCAACGCTGCAAGCTGATGGCCTGGAACAACTTGCCGTGGACGGAGCCAAGGCAGGCCTT
AGCAATCTTCTCGATCAGCTGGATCGCACTTCCTGGGACGGTTTTGATGTCAAGCAAGCACAACAACTGCTAACGAATGC
TGCTCAGGCTGCCAACGTCAAAAAAGGTGTGATCATGAAAAGCCTCAGAGCTGCGCTTCTAGGCCGCCTACAAGGGCCAG
ACCTAATCACAACATGGGGATTACTGGCCAGGATCGGGCAAGACTTGAACAGGCTGCGTCGTTGTCTCTGA

Upstream 100 bases:

>100_bases
GGACCGTAGTTCAATTGGTTAGAGCACCGCCCTGTCACGGCGGAAGTTGCGGGTTCGAACCCCGTCGGTCCCGTTCTTAT
TCCTAATCCTGAGAGTCATT

Downstream 100 bases:

>100_bases
GCAGTCGTCTTCGTTTGAATCTTCAGGAGCGATTAAACCCAGGGCCTTGGCCAGCGGCTGGGCCGTGAGGCCTTGAATGC
CAACTGTCATCAGGATGGTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGQFLLRIEDTDKERSKPEFTTNILEGLKWLGLNWDEQPIIQSQHVD
DHRAAIQKLLDRDLAYRCYASETELEAMRETQKAQGKAPRYDNRHRDLNPEQEAAFQSEGRTAVVRFRIDDDATISWKDL
VRGPMHWKGSDLGGDMVISRRAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMSLLGWSVPEGTDERFTLQQAATVFSFDRVNKAGAKFDWDKLNWLN
SQVLHDLPKDQLLHELKPLWSKAGWALPEENWCLDLAELLGPSLTLLKDGVDQARPFFEEPTLQADGLEQLAVDGAKAGL
SNLLDQLDRTSWDGFDVKQAQQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL

Sequences:

>Translated_476_residues
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGQFLLRIEDTDKERSKPEFTTNILEGLKWLGLNWDEQPIIQSQHVD
DHRAAIQKLLDRDLAYRCYASETELEAMRETQKAQGKAPRYDNRHRDLNPEQEAAFQSEGRTAVVRFRIDDDATISWKDL
VRGPMHWKGSDLGGDMVISRRAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMSLLGWSVPEGTDERFTLQQAATVFSFDRVNKAGAKFDWDKLNWLN
SQVLHDLPKDQLLHELKPLWSKAGWALPEENWCLDLAELLGPSLTLLKDGVDQARPFFEEPTLQADGLEQLAVDGAKAGL
SNLLDQLDRTSWDGFDVKQAQQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL
>Mature_476_residues
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGQFLLRIEDTDKERSKPEFTTNILEGLKWLGLNWDEQPIIQSQHVD
DHRAAIQKLLDRDLAYRCYASETELEAMRETQKAQGKAPRYDNRHRDLNPEQEAAFQSEGRTAVVRFRIDDDATISWKDL
VRGPMHWKGSDLGGDMVISRRAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMSLLGWSVPEGTDERFTLQQAATVFSFDRVNKAGAKFDWDKLNWLN
SQVLHDLPKDQLLHELKPLWSKAGWALPEENWCLDLAELLGPSLTLLKDGVDQARPFFEEPTLQADGLEQLAVDGAKAGL
SNLLDQLDRTSWDGFDVKQAQQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=476, Percent_Identity=34.0336134453782, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI62241042, Length=344, Percent_Identity=22.6744186046512, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788743, Length=485, Percent_Identity=37.9381443298969, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI226510925, Length=275, Percent_Identity=32.7272727272727, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1786895, Length=318, Percent_Identity=22.9559748427673, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=299, Percent_Identity=36.7892976588629, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17511007, Length=365, Percent_Identity=24.1095890410959, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=30.9716599190283, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=26.6094420600858, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=133, Percent_Identity=30.8270676691729, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=33.9960238568588, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53387; Mature: 53387

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGQFLLRIEDTDKERSKPEFTTNILEG
CEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LKWLGLNWDEQPIIQSQHVDDHRAAIQKLLDRDLAYRCYASETELEAMRETQKAQGKAPR
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
YDNRHRDLNPEQEAAFQSEGRTAVVRFRIDDDATISWKDLVRGPMHWKGSDLGGDMVISR
CCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEEHHHHHCCCCCCCCCCCCCCEEEEC
RAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
CCCHHHHCCHHEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMSLLGWSVPEGTDERFTLQQAATVFS
EECCCCCCCHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
FDRVNKAGAKFDWDKLNWLNSQVLHDLPKDQLLHELKPLWSKAGWALPEENWCLDLAELL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
GPSLTLLKDGVDQARPFFEEPTLQADGLEQLAVDGAKAGLSNLLDQLDRTSWDGFDVKQA
CCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVRVRLAPSPTGTLHIGTARTAVFNWLFARHQGGQFLLRIEDTDKERSKPEFTTNILEG
CEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LKWLGLNWDEQPIIQSQHVDDHRAAIQKLLDRDLAYRCYASETELEAMRETQKAQGKAPR
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
YDNRHRDLNPEQEAAFQSEGRTAVVRFRIDDDATISWKDLVRGPMHWKGSDLGGDMVISR
CCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEEHHHHHCCCCCCCCCCCCCCEEEEC
RAPAKEIGDPLYNLVVVVDDAAMSISHVIRGEDHIANTAKQLLIYEALGLPIPQFAHTPL
CCCHHHHCCHHEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILNSEGRKLSKRDGVTSISDFQAMGYTAEAMANYMSLLGWSVPEGTDERFTLQQAATVFS
EECCCCCCCHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
FDRVNKAGAKFDWDKLNWLNSQVLHDLPKDQLLHELKPLWSKAGWALPEENWCLDLAELL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
GPSLTLLKDGVDQARPFFEEPTLQADGLEQLAVDGAKAGLSNLLDQLDRTSWDGFDVKQA
CCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QQLLTNAAQAANVKKGVIMKSLRAALLGRLQGPDLITTWGLLARIGQDLNRLRRCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA