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The map label for this gene is proS [H]

Identifier: 33863527

GI number: 33863527

Start: 1348272

End: 1350071

Strand: Direct

Name: proS [H]

Synonym: PMT1259

Alternate gene names: 33863527

Gene position: 1348272-1350071 (Clockwise)

Preceding gene: 33863523

Following gene: 33863528

Centisome position: 55.92

GC content: 54.56

Gene sequence:

>1800_bases
ATGCGCGTCTCCCGCCTGATGCTGGTGACGTTGCGCGACATACCAGCTGACGCGGAAATCGCCTCCCATCAATTGCTGCT
CAGAGGGGGATATATCCGTCGTGTGACTTCAGGCATTTATGCCTACCTACCACTGATGTGGCGGGTCCTGCGCAAAATCA
CATCGATTGTTCAGGAGGAAATGGATGCCACTGGTGCTCTGGAGACCTTGTTACCTCAATTGCAGCCTGCAGAGCTCTGG
CGGCGTAGTGGCCGATGGCAGGGGTATACGGCCGGCGAAGGCCTGATGTTCCACCTTGAAGACCGCCAAGGACGAGAACT
CGGACTCGGCCCCACCCATGAAGAGGTGATCACAAGTCTTGCCGGAGACCTGCTGCGCTCATATCGACAGCTTCCTGTAA
CGCTCTATCAGATCCAAAGCAAATTCCGCGATGAGATCCGCCCACGTTTTGGCCTGATGCGCGGCCGCGAATTCATCATG
AAGGACGCCTATTCCTTCCATTCCTGCGAAGCAGACCTCGCCATGACCTACAGAGCCATGGATGAGGCCTATCAACGAAT
CTTCTCCCGTTGCGGACTCGAAACCGTCGCCGTGGAAGCCGACAGCGGAGCCATCGGCGGAGCCTCGTCTCAAGAATTTA
TGGTCACAGCGGATGCTGGCGAAGACCTGATCCTGATCAGTGGTGATGGCCACTACGCCGCCAACCAAGAAAAGGCCGTC
TCTCATCCACCAAAAGCCATCCCCCTGCCTGCTTCAAAAGTTGCCTTGCTTGATACACCCGAACAGGGAACGATCGAGAC
ACTGTGTACCGCCCAGGGCCTTGTTCCGTCTCAAGTGGTGAAGGTGCTGGTCATGCTGGCCCGCTTCGAAGATGGTGAAC
TTCAACCAGTCCTTGTAAGCATCCGAGGAGACCAGCAACTCAATGAGGTAAAGCTGATCAACGCCCTAAGCAGGGAACTA
AACAAGGGGGTGCTCGATGTGGCCCCCATCAGCGCCGATCAAATCACTGCGCAAAAGCTTGAAGCCTGGCCCTTTGGGGC
GATAGGGCCTGACCTAGACGATGCCCTGTTAAGCGGAGCCACAAGCTGGACAAAACATTTCCTACGCCTAGCTGACCCGA
CAGCTACTGAGCTGAACTGTTTTGTCTGCGGTGCCAATCAAAACAATCAGCATCGAACTGGCATGACCTGGTCAAAACTC
GGGGGAGTGCCCAAAAGCGTCGATCTACGCAAATCGCAGGCTGGCGATCGCTGTATCCATGACTCCAGCCAGATTCTGGA
GGAGAGAAGAGGCATTGAGGTAGGACACATTTTCCAATTAGGTCGCAAGTACTCAGAAGCCCTAGAGGCCTGTTTCACCA
ATGACCAGGGCACTCAGGAGCCTTTCTGGATGGGGTGTTACGGCATCGGGATTTCCAGACTTGCCCAGGCTGCTGTGGAA
CAACACCATGACGAAGCTGGCATGAAATGGCCTCTGGCTATAGCCCCATTTGAGGTCATCATCGTGGTAGCCAACATCCA
GGATGAGGTTCAGCAAAAGCTGGCCGAACAGCTTTACAGCGAACTACAAGCAGCTGATATCGAAGCACTACTTGATGACA
GGACTGAACGCGCCGGCGTGAAATTCAAAGATGCTGACCTGATCGGTATCCCTTGGCGAGTCGTGGTTGGCAGAGATGCC
GCCAAGGGGCAGGTGGAACTGATTCAAAGATCCAGCCGCAAGGTTCAAATACTTTCTGCAAAACAGGCCTTTACCGCACT
GGTTAAAGACATAGCAGCGAACCAAAACACAAGGGTCTGA

Upstream 100 bases:

>100_bases
ACTCATCTCGTACAGGTCGATTAGGCGCTTTGAGACAGTTTCGTGATCGGCTTATTTGGACCGCGGATGGTAGCTTCCCC
CATCCACTGAGGTCCGATTC

Downstream 100 bases:

>100_bases
CCAGCCATTTAAAATTCTTGGGAATGGAATGAAACGGATGACTACTGCCTTGCATCACATGGCCAAGCAGTTGATGCGGG
CAGGATTGGCAATCTGCCTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MRVSRLMLVTLRDIPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITSIVQEEMDATGALETLLPQLQPAELW
RRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSLAGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIM
KDAYSFHSCEADLAMTYRAMDEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGHYAANQEKAV
SHPPKAIPLPASKVALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARFEDGELQPVLVSIRGDQQLNEVKLINALSREL
NKGVLDVAPISADQITAQKLEAWPFGAIGPDLDDALLSGATSWTKHFLRLADPTATELNCFVCGANQNNQHRTGMTWSKL
GGVPKSVDLRKSQAGDRCIHDSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDQGTQEPFWMGCYGIGISRLAQAAVE
QHHDEAGMKWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEALLDDRTERAGVKFKDADLIGIPWRVVVGRDA
AKGQVELIQRSSRKVQILSAKQAFTALVKDIAANQNTRV

Sequences:

>Translated_599_residues
MRVSRLMLVTLRDIPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITSIVQEEMDATGALETLLPQLQPAELW
RRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSLAGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIM
KDAYSFHSCEADLAMTYRAMDEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGHYAANQEKAV
SHPPKAIPLPASKVALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARFEDGELQPVLVSIRGDQQLNEVKLINALSREL
NKGVLDVAPISADQITAQKLEAWPFGAIGPDLDDALLSGATSWTKHFLRLADPTATELNCFVCGANQNNQHRTGMTWSKL
GGVPKSVDLRKSQAGDRCIHDSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDQGTQEPFWMGCYGIGISRLAQAAVE
QHHDEAGMKWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEALLDDRTERAGVKFKDADLIGIPWRVVVGRDA
AKGQVELIQRSSRKVQILSAKQAFTALVKDIAANQNTRV
>Mature_599_residues
MRVSRLMLVTLRDIPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITSIVQEEMDATGALETLLPQLQPAELW
RRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSLAGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIM
KDAYSFHSCEADLAMTYRAMDEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGHYAANQEKAV
SHPPKAIPLPASKVALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARFEDGELQPVLVSIRGDQQLNEVKLINALSREL
NKGVLDVAPISADQITAQKLEAWPFGAIGPDLDDALLSGATSWTKHFLRLADPTATELNCFVCGANQNNQHRTGMTWSKL
GGVPKSVDLRKSQAGDRCIHDSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDQGTQEPFWMGCYGIGISRLAQAAVE
QHHDEAGMKWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEALLDDRTERAGVKFKDADLIGIPWRVVVGRDA
AKGQVELIQRSSRKVQILSAKQAFTALVKDIAANQNTRV

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=231, Percent_Identity=43.7229437229437, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI1786392, Length=594, Percent_Identity=44.2760942760943, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI115532348, Length=207, Percent_Identity=39.1304347826087, Blast_Score=148, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6320931, Length=226, Percent_Identity=37.1681415929204, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24656200, Length=228, Percent_Identity=37.719298245614, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 66260; Mature: 66260

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSRLMLVTLRDIPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITSIVQEE
CCCHHEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDATGALETLLPQLQPAELWRRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSL
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
AGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIMKDAYSFHSCEADLAMTYRAM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGHYAANQEKAV
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCC
SHPPKAIPLPASKVALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARFEDGELQPVLVS
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IRGDQQLNEVKLINALSRELNKGVLDVAPISADQITAQKLEAWPFGAIGPDLDDALLSGA
ECCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
TSWTKHFLRLADPTATELNCFVCGANQNNQHRTGMTWSKLGGVPKSVDLRKSQAGDRCIH
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCHHHCCCCHHHC
DSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDQGTQEPFWMGCYGIGISRLAQAAVE
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHH
QHHDEAGMKWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEALLDDRTERAGV
HHHHHCCCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
KFKDADLIGIPWRVVVGRDAAKGQVELIQRSSRKVQILSAKQAFTALVKDIAANQNTRV
EECCCCEECCCEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRVSRLMLVTLRDIPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITSIVQEE
CCCHHEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDATGALETLLPQLQPAELWRRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSL
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
AGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIMKDAYSFHSCEADLAMTYRAM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGHYAANQEKAV
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCC
SHPPKAIPLPASKVALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARFEDGELQPVLVS
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IRGDQQLNEVKLINALSRELNKGVLDVAPISADQITAQKLEAWPFGAIGPDLDDALLSGA
ECCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
TSWTKHFLRLADPTATELNCFVCGANQNNQHRTGMTWSKLGGVPKSVDLRKSQAGDRCIH
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCHHHCCCCHHHC
DSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDQGTQEPFWMGCYGIGISRLAQAAVE
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHH
QHHDEAGMKWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEALLDDRTERAGV
HHHHHCCCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
KFKDADLIGIPWRVVVGRDAAKGQVELIQRSSRKVQILSAKQAFTALVKDIAANQNTRV
EECCCCEECCCEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA