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The map label for this gene is cobB [H]

Identifier: 33863453

GI number: 33863453

Start: 1273438

End: 1274943

Strand: Direct

Name: cobB [H]

Synonym: PMT1183

Alternate gene names: 33863453

Gene position: 1273438-1274943 (Clockwise)

Preceding gene: 33863452

Following gene: 33863454

Centisome position: 52.82

GC content: 53.19

Gene sequence:

>1506_bases
ATGTCGACCCCCCTGATGGTGTTAGGCACATCCAGTGGAGCTGGTAAATCATTAATGTCGGCCGCTCTCTGCAGGCTGCT
GCTTCGTCAAGGCGAAACTCCCATACCCTTCAAAGGGCAAAACATGAGCAACAACGCCTGGGTTGATGAGAGCGGCGGAG
AGATGGCCTATTCACAAGCCCTGCAAGCTTGGGCGGCAGGACTCAAGCCCCAATGCGCCATGAACCCTGTGCTGCTCAAA
CCCCAGGGAGACAGCACCAGTGAAGTCATCCATCTGGGTCAAAGCGTTGGCACGGCGCGTGCCGAGACCTACTACGAGCA
ATGGTTCCGTCCTGGATGGAGCGTCATCCGTGAGGCGCTAAAAGACCTTCAATGCTCTTACACCCATGGCCGGCTTGTCT
TGGAAGGTGCAGGCAGCCCAGTGGAAGTCAACCTGCAACGGCGAGACCTCACCAACCTGCGATTGGCTCAGTTCCTGCGT
GCCCGCTGTTTGCTCGTTGCCGATATCGAACGCGGTGGTGTTTTTGCCCAGATCATGGGAACCCTGTCTTTACTGAAGCC
GGTAGAAAAGCAGTTGATACGCGGGCTACTCATCAATCGTTTCCGTGGCCGACGCGAACTTTTTGATGAAGGGCGCGATT
GGTTAGAAGCCGAAACCGGAATTCCCGTCATCGGTGTAATGCCATGGCTGGAAGAGCTCTTCCCGCCAGAAGACTCCCTT
GATCTACTGGAGAGAAGAGGCAAAAAAAATGATGCCGAGATCGAAATCGCTGTCTTGAAATTGCCATCCCTAAGTAATTT
TTCCGATCTCGATCCATTAGAAGCAGAACCCACAGTTCAGCTGCGATGGGTTGAACCCGGCACATTCCTCGGGATGCCAG
ATGCTGTCATCATTCCCGGCAGCAAGCAGACACTGCGCGATCTAAATTCGCTAAACCGCAGTGGTTTAGGAAGCCAGTTG
CAAACCTACGCCCAAAGCGGCGGGCATGTGTTTGGCATCTGTGGCGGCATGCAAATGCTTGGGCGAACTCTGGCAGATCC
CCTAGGTCTGGAAGGCGTCACAACAACAGACCCATCAAGCAGCATGGCAGGACTCAATCTGCTGCCTCTAGATACCGTTT
TCAAACGCAACAAGGCTCTAAGTCAATGCCAAAGGATTACCCAGTGGCCAGACAATGCCAAGGTGAAGGGGTTTGAATTA
CACCATGGCAATAGCCAACTCATTCAAGGGAGCCATGAGTCTGTCCTCCCTATGGCTGACGATCCCTCAATGGGTTGGGT
CAGTAAAAATGAGAGCTGCGGCCAAGTCGCGGGAACTTACCTGCACGGGATTTTCGAGAATGGTCGCTGGCGAAGGCTGT
GGCTCAATCTGATACGGAAGCAAAAGGGTTTAGCAGACTTGCCGACCGATATATCCAACCATGAGCAACAAAGAGAACAA
CTACTCAATCGTCTCGCCGATGTTTTCGAAGAACATGTCAACATCAACCCACTGCTGGGAACATGA

Upstream 100 bases:

>100_bases
TTGAACAGTCACCAGGGTTACCAACACAACAAGCTGGGATTTTCGAACGACTGCAACACAATCTGCGTCGACTTTGGCCC
AGACAATGAGCACCGATGGG

Downstream 100 bases:

>100_bases
CGATCTCTAAGATTGAGATTCAATGGCCCAATGGCACAAGATCCCATACAGAACCAGGGATTGATTGGCTCCAGGTTGCT
CGAGACGCAGGAATCATCAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSTPLMVLGTSSGAGKSLMSAALCRLLLRQGETPIPFKGQNMSNNAWVDESGGEMAYSQALQAWAAGLKPQCAMNPVLLK
PQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLAQFLR
ARCLLVADIERGGVFAQIMGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFPPEDSL
DLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGMPDAVIIPGSKQTLRDLNSLNRSGLGSQL
QTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSSSMAGLNLLPLDTVFKRNKALSQCQRITQWPDNAKVKGFEL
HHGNSQLIQGSHESVLPMADDPSMGWVSKNESCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDISNHEQQREQ
LLNRLADVFEEHVNINPLLGT

Sequences:

>Translated_501_residues
MSTPLMVLGTSSGAGKSLMSAALCRLLLRQGETPIPFKGQNMSNNAWVDESGGEMAYSQALQAWAAGLKPQCAMNPVLLK
PQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLAQFLR
ARCLLVADIERGGVFAQIMGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFPPEDSL
DLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGMPDAVIIPGSKQTLRDLNSLNRSGLGSQL
QTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSSSMAGLNLLPLDTVFKRNKALSQCQRITQWPDNAKVKGFEL
HHGNSQLIQGSHESVLPMADDPSMGWVSKNESCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDISNHEQQREQ
LLNRLADVFEEHVNINPLLGT
>Mature_500_residues
STPLMVLGTSSGAGKSLMSAALCRLLLRQGETPIPFKGQNMSNNAWVDESGGEMAYSQALQAWAAGLKPQCAMNPVLLKP
QGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLAQFLRA
RCLLVADIERGGVFAQIMGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFPPEDSLD
LLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGMPDAVIIPGSKQTLRDLNSLNRSGLGSQLQ
TYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSSSMAGLNLLPLDTVFKRNKALSQCQRITQWPDNAKVKGFELH
HGNSQLIQGSHESVLPMADDPSMGWVSKNESCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDISNHEQQREQL
LNRLADVFEEHVNINPLLGT

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 55321; Mature: 55190

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLMVLGTSSGAGKSLMSAALCRLLLRQGETPIPFKGQNMSNNAWVDESGGEMAYSQA
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHH
LQAWAAGLKPQCAMNPVLLKPQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREAL
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHH
KDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLAQFLRARCLLVADIERGGVFAQIMG
HHHCCCCCCCEEEEECCCCCEEEEEEHHCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHH
TLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFPPEDSL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHCCCCCCH
DLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGMPDAVIIPG
HHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCEEEECC
SKQTLRDLNSLNRSGLGSQLQTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSS
CHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCC
SMAGLNLLPLDTVFKRNKALSQCQRITQWPDNAKVKGFELHHGNSQLIQGSHESVLPMAD
CCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCC
DPSMGWVSKNESCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDISNHEQQREQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LLNRLADVFEEHVNINPLLGT
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STPLMVLGTSSGAGKSLMSAALCRLLLRQGETPIPFKGQNMSNNAWVDESGGEMAYSQA
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHH
LQAWAAGLKPQCAMNPVLLKPQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREAL
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHH
KDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLAQFLRARCLLVADIERGGVFAQIMG
HHHCCCCCCCEEEEECCCCCEEEEEEHHCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHH
TLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFPPEDSL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHCCCCCCH
DLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGMPDAVIIPG
HHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCEEEECC
SKQTLRDLNSLNRSGLGSQLQTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSS
CHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCC
SMAGLNLLPLDTVFKRNKALSQCQRITQWPDNAKVKGFELHHGNSQLIQGSHESVLPMAD
CCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCC
DPSMGWVSKNESCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDISNHEQQREQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LLNRLADVFEEHVNINPLLGT
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA