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The map label for this gene is mhpC [H]

Identifier: 33863445

GI number: 33863445

Start: 1266708

End: 1267706

Strand: Direct

Name: mhpC [H]

Synonym: PMT1175

Alternate gene names: 33863445

Gene position: 1266708-1267706 (Clockwise)

Preceding gene: 33863440

Following gene: 33863446

Centisome position: 52.54

GC content: 54.05

Gene sequence:

>999_bases
TTGGTTCAAGCCGCTTTGTTTGCAACTGACTTCCAGACTGCCGAGCGGGCCATTCAAGAGACAGGTGCTGACTGGGGCGA
ATCCCATGATTGGTATTGGCAAGGCCTACGTTGTCACTGGCGTGTACTGGGAGCAGAGGATGCACGGCCACTCGTGCTCA
TCCACGGATTCGGTGCCAGCAGCGCTCATTGGCGACACAATGCTGCACCCCTAAAAGCGGCAGGATTCCGCGTCTACAGC
ATTGATCTGATCGGTTTTGGAGCTTCAGCCCAGCCAGGACTGAAGCAATTTCGACGCCTCGACAATCGACTGTGGGGCAA
ACAATTAGCAGCCTTTCTTGAGCAAATTGTCCAGGCCAGCCCCTCTCAACAAGCAGTACTAATAGGCAATTCTCTAGGAG
CACTAACGGCTCTCACCACAGCCGCATTCCGCCCAGACCTTGTTGGAGCTGTGGTAGCAGCTCCATTACCGGATCCTGCA
TTGGTTGAACCATTGAGCTTCCACCCCACCCGCTGGTGGCGCCGGTTAAAGCACAAGTTGATCGCAATCATCTTCCGCCT
CTTGCCTCTGGAACTGATTGTTCCTCTCATCGCACGAACGCCACTCCTTAAAGCAGCTCTTCAAATGGGCTACCACCACT
CCATCCATTTAGACCATGAGCTACTACACCTGATCGCCCAACCTGCAAGGCGACCCTCGGCAGCTCGCGCCCTACGAGCG
ATGTGCGTTGGCATGACACTGCGGCCAGTGGGCTGCACAGCTCCTCGCTTACTAAAACGCCTTGCTAGCAGGCCCGATCG
CCCCCCCATATTGCTTCTCTGGGGACGAGAGGACCGTTTTGTGCCATTAAAAATTGGCCATCGACTGCAGCATCAATACC
CATGGATAACGCTTTCAGTGCTTAATGAAACTGGCCATTGCCCCCATGACGAATCCACCCAGGCTTTTGATCAAGCCGTG
TTCAGCTGGCTGAACCTTAATTTAGGTAATGAACAATAA

Upstream 100 bases:

>100_bases
CCAATAAAACCGAAAGATCACGGGAAGGACGAGACTCTGGGCGGAAGAAACCACCTCCTGGCTCCAAAACGACTGAGCCT
TCGCGATAGTGGGTGGTGGG

Downstream 100 bases:

>100_bases
AGGGCATGAAGCACACCCTTTCAGTGCTAGTCGAAGACGAATCCGGAGCTCTAAGCCGGATCGCAGGACTTTTTGCACGC
CGTGGTTTCAACATCGACAG

Product: alpha/beta hydrolase superfamily protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MVQAALFATDFQTAERAIQETGADWGESHDWYWQGLRCHWRVLGAEDARPLVLIHGFGASSAHWRHNAAPLKAAGFRVYS
IDLIGFGASAQPGLKQFRRLDNRLWGKQLAAFLEQIVQASPSQQAVLIGNSLGALTALTTAAFRPDLVGAVVAAPLPDPA
LVEPLSFHPTRWWRRLKHKLIAIIFRLLPLELIVPLIARTPLLKAALQMGYHHSIHLDHELLHLIAQPARRPSAARALRA
MCVGMTLRPVGCTAPRLLKRLASRPDRPPILLLWGREDRFVPLKIGHRLQHQYPWITLSVLNETGHCPHDESTQAFDQAV
FSWLNLNLGNEQ

Sequences:

>Translated_332_residues
MVQAALFATDFQTAERAIQETGADWGESHDWYWQGLRCHWRVLGAEDARPLVLIHGFGASSAHWRHNAAPLKAAGFRVYS
IDLIGFGASAQPGLKQFRRLDNRLWGKQLAAFLEQIVQASPSQQAVLIGNSLGALTALTTAAFRPDLVGAVVAAPLPDPA
LVEPLSFHPTRWWRRLKHKLIAIIFRLLPLELIVPLIARTPLLKAALQMGYHHSIHLDHELLHLIAQPARRPSAARALRA
MCVGMTLRPVGCTAPRLLKRLASRPDRPPILLLWGREDRFVPLKIGHRLQHQYPWITLSVLNETGHCPHDESTQAFDQAV
FSWLNLNLGNEQ
>Mature_332_residues
MVQAALFATDFQTAERAIQETGADWGESHDWYWQGLRCHWRVLGAEDARPLVLIHGFGASSAHWRHNAAPLKAAGFRVYS
IDLIGFGASAQPGLKQFRRLDNRLWGKQLAAFLEQIVQASPSQQAVLIGNSLGALTALTTAAFRPDLVGAVVAAPLPDPA
LVEPLSFHPTRWWRRLKHKLIAIIFRLLPLELIVPLIARTPLLKAALQMGYHHSIHLDHELLHLIAQPARRPSAARALRA
MCVGMTLRPVGCTAPRLLKRLASRPDRPPILLLWGREDRFVPLKIGHRLQHQYPWITLSVLNETGHCPHDESTQAFDQAV
FSWLNLNLGNEQ

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=298, Percent_Identity=25.8389261744966, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 37137; Mature: 37137

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQAALFATDFQTAERAIQETGADWGESHDWYWQGLRCHWRVLGAEDARPLVLIHGFGAS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCCCEEEEEECCCC
SAHWRHNAAPLKAAGFRVYSIDLIGFGASAQPGLKQFRRLDNRLWGKQLAAFLEQIVQAS
CCCCCCCCCCCHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSQQAVLIGNSLGALTALTTAAFRPDLVGAVVAAPLPDPALVEPLSFHPTRWWRRLKHKL
CCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHH
IAIIFRLLPLELIVPLIARTPLLKAALQMGYHHSIHLDHELLHLIAQPARRPSAARALRA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCHHHHHHHH
MCVGMTLRPVGCTAPRLLKRLASRPDRPPILLLWGREDRFVPLKIGHRLQHQYPWITLSV
HHHCCEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHHHCCCEEEEEE
LNETGHCPHDESTQAFDQAVFSWLNLNLGNEQ
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVQAALFATDFQTAERAIQETGADWGESHDWYWQGLRCHWRVLGAEDARPLVLIHGFGAS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEEECCCCCCCEEEEEECCCC
SAHWRHNAAPLKAAGFRVYSIDLIGFGASAQPGLKQFRRLDNRLWGKQLAAFLEQIVQAS
CCCCCCCCCCCHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSQQAVLIGNSLGALTALTTAAFRPDLVGAVVAAPLPDPALVEPLSFHPTRWWRRLKHKL
CCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHH
IAIIFRLLPLELIVPLIARTPLLKAALQMGYHHSIHLDHELLHLIAQPARRPSAARALRA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCHHHHHHHH
MCVGMTLRPVGCTAPRLLKRLASRPDRPPILLLWGREDRFVPLKIGHRLQHQYPWITLSV
HHHCCEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHHHCCCEEEEEE
LNETGHCPHDESTQAFDQAVFSWLNLNLGNEQ
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA