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The map label for this gene is hisH

Identifier: 33863399

GI number: 33863399

Start: 1221554

End: 1222195

Strand: Direct

Name: hisH

Synonym: PMT1128

Alternate gene names: 33863399

Gene position: 1221554-1222195 (Clockwise)

Preceding gene: 33863398

Following gene: 33863400

Centisome position: 50.67

GC content: 56.23

Gene sequence:

>642_bases
TTGATTACACGTCTAGGCTTAATTGATTACGGCATGGGCAATCTGCGCTCTGTGCAGATTGCCTTTGAAAGGCTCCACAA
GGCTCTTCACATAGTGAGGCAGCCCGCCGACCTCAGCCGCTGTGATGCCTTGATCTTGCCTGGAGTCGGTGCCTTTGATC
CCGCCATGGTGCACCTTGAGCAGACCAGATTGGTTCCAGACCTCAAGAGTTGGGTGAAAGGAGGACGACCTCTGCTAGGC
ATCTGTCTGGGCCTGCAGTTGTTGTTCGAATCGAGTGACGAAGGAAATGCCACCGGCCTAGGGTTGCTTAAGGGCCACGT
TCAACGACTGCCCTCCAATCAAGGCGAACGCATTCCCCACATGGGCTGGGCTGCTCTAGAACATCGAAACGACTGCCCGC
TGCTTGACAAGGAAGATCCAGACAGCTGGATGTATTTCGTGCATTCCTATGCTGCAGTCCCCAGCCAAAACAGTGATCTT
GCGGCCATAGCGCCGTTTGGCCGAGACAACATCACAGCGATGGTCTGGAAAGGCCGACTAGGGGCCTGTCAGTTTCACCC
AGAGAAATCCGCCGCAGCCGGCGAGCGCATGCTGCGGCGTTGGCTGAATTGGCTGGAGACCGGCGCAAAACCTGTGCCAT
GA

Upstream 100 bases:

>100_bases
CTCTGAAGCTTGATCCAACTTCGATTCCAGCCCTCCTAGATCACCATTGGCCTGATTCAATGCCTCACATGGCTAAAAGG
CCAGATTGCGGAGGGTTTCT

Downstream 100 bases:

>100_bases
GTAGCCAGCTAAGGCTTTCCGGAGGGCGCAAGCTTCAGAGTCCCGCTGGCCAAGGCACTAGACCCACGACGAGTCGTGTT
CGTGAAGCAGTGATGAACGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MITRLGLIDYGMGNLRSVQIAFERLHKALHIVRQPADLSRCDALILPGVGAFDPAMVHLEQTRLVPDLKSWVKGGRPLLG
ICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPHMGWAALEHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDL
AAIAPFGRDNITAMVWKGRLGACQFHPEKSAAAGERMLRRWLNWLETGAKPVP

Sequences:

>Translated_213_residues
MITRLGLIDYGMGNLRSVQIAFERLHKALHIVRQPADLSRCDALILPGVGAFDPAMVHLEQTRLVPDLKSWVKGGRPLLG
ICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPHMGWAALEHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDL
AAIAPFGRDNITAMVWKGRLGACQFHPEKSAAAGERMLRRWLNWLETGAKPVP
>Mature_213_residues
MITRLGLIDYGMGNLRSVQIAFERLHKALHIVRQPADLSRCDALILPGVGAFDPAMVHLEQTRLVPDLKSWVKGGRPLLG
ICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPHMGWAALEHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDL
AAIAPFGRDNITAMVWKGRLGACQFHPEKSAAAGERMLRRWLNWLETGAKPVP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=35.0253807106599, Blast_Score=100, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=32.8828828828829, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS52_PROMM (Q7V6M5)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894959.1
- ProteinModelPortal:   Q7V6M5
- SMR:   Q7V6M5
- STRING:   Q7V6M5
- GeneID:   1729583
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT1128
- NMPDR:   fig|74547.1.peg.1126
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13141
- BioCyc:   PMAR74547:PMT1128-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23577; Mature: 23577

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRLGLIDYGMGNLRSVQIAFERLHKALHIVRQPADLSRCDALILPGVGAFDPAMVHLE
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCHHHHHHH
QTRLVPDLKSWVKGGRPLLGICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPH
HHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
MGWAALEHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDLAAIAPFGRDNITAMVWKGRL
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCC
GACQFHPEKSAAAGERMLRRWLNWLETGAKPVP
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITRLGLIDYGMGNLRSVQIAFERLHKALHIVRQPADLSRCDALILPGVGAFDPAMVHLE
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCHHHHHHH
QTRLVPDLKSWVKGGRPLLGICLGLQLLFESSDEGNATGLGLLKGHVQRLPSNQGERIPH
HHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
MGWAALEHRNDCPLLDKEDPDSWMYFVHSYAAVPSQNSDLAAIAPFGRDNITAMVWKGRL
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCC
GACQFHPEKSAAAGERMLRRWLNWLETGAKPVP
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642