Click here to switch to the map view.

The map label for this gene is mscL [H]

Identifier: 33863128

GI number: 33863128

Start: 937493

End: 937885

Strand: Reverse

Name: mscL [H]

Synonym: PMT0856

Alternate gene names: 33863128

Gene position: 937885-937493 (Counterclockwise)

Preceding gene: 33863131

Following gene: 33863127

Centisome position: 38.9

GC content: 47.84

Gene sequence:

>393_bases
ATGGCATACCGTCGCAAAATAACCAACGTCCTTCATGACTTCACTGAATTTATCAATCGCGGCAATGTCGTTGATCTAGC
GGTCGCTGTTGTCGTAGGTGGTGCCTTCTCCAAGGTGGTGGATGCACTTGTCAAGTTGGTCACTATCGCTGCGATGGACC
CTCTACTTCGTAAACTTAAGGTAGAGACACTAAGTGACTTGCCCGGCGGATCCTTGCTGGTTGCAATAATCAATTTCTTG
GTCATTGCCTTCGTGGTATTTATTGTGGTGAGGGCTATCGAGCGCTTCAAACGCAAACAAGAGGTTAAAGCTGTAGCAGG
TCCAAATACCCAACAGCAATTAGCCGAAGCGGTTGAACGTTTAGCAATTGCACTGGAGAGCCGTGAGCTTTGA

Upstream 100 bases:

>100_bases
CCCTGAAGATTTAGAAGGGGTAAATCGTTTAAATTTTTTAGGATTGTGGCGTCATCTCACTGAGAGGTTCATTATGGTGT
CTTTTTAGTCATTGCTTCAT

Downstream 100 bases:

>100_bases
GCTCATCAAACTCTTATAAAACTAGTTTAGGTTTCTTAATGCACTGAACCGCTTCTGCTTGATTGGCTGCCATTTTTGGT
GTATCACCTGAACATCCCTA

Product: large-conductance mechanosensitive channel mscL

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MAYRRKITNVLHDFTEFINRGNVVDLAVAVVVGGAFSKVVDALVKLVTIAAMDPLLRKLKVETLSDLPGGSLLVAIINFL
VIAFVVFIVVRAIERFKRKQEVKAVAGPNTQQQLAEAVERLAIALESREL

Sequences:

>Translated_130_residues
MAYRRKITNVLHDFTEFINRGNVVDLAVAVVVGGAFSKVVDALVKLVTIAAMDPLLRKLKVETLSDLPGGSLLVAIINFL
VIAFVVFIVVRAIERFKRKQEVKAVAGPNTQQQLAEAVERLAIALESREL
>Mature_129_residues
AYRRKITNVLHDFTEFINRGNVVDLAVAVVVGGAFSKVVDALVKLVTIAAMDPLLRKLKVETLSDLPGGSLLVAIINFLV
IAFVVFIVVRAIERFKRKQEVKAVAGPNTQQQLAEAVERLAIALESREL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=119, Percent_Identity=31.9327731092437, Blast_Score=66, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14269; Mature: 14138

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYRRKITNVLHDFTEFINRGNVVDLAVAVVVGGAFSKVVDALVKLVTIAAMDPLLRKLK
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VETLSDLPGGSLLVAIINFLVIAFVVFIVVRAIERFKRKQEVKAVAGPNTQQQLAEAVER
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LAIALESREL
HHHHHHHCCC
>Mature Secondary Structure 
AYRRKITNVLHDFTEFINRGNVVDLAVAVVVGGAFSKVVDALVKLVTIAAMDPLLRKLK
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VETLSDLPGGSLLVAIINFLVIAFVVFIVVRAIERFKRKQEVKAVAGPNTQQQLAEAVER
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LAIALESREL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]