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The map label for this gene is prfC [H]

Identifier: 33863063

GI number: 33863063

Start: 864375

End: 866090

Strand: Reverse

Name: prfC [H]

Synonym: PMT0791

Alternate gene names: 33863063

Gene position: 866090-864375 (Counterclockwise)

Preceding gene: 33863064

Following gene: 33863062

Centisome position: 35.92

GC content: 48.72

Gene sequence:

>1716_bases
ATGCACTTGCACCCGCCAGATCTTCGTCACAGCTTGACCTCTCTTCCCGATTCCACGAGCCCGCCGATTGATTCGGCTTC
TTCAATCGATATAGATCAAGTCCTTGAGGCCTCGTTGGCTGAGGCCATTAGCCGTCGCCGCAATTTCGCGATCATTTCCC
ATCCAGATGCGGGGAAGACCACCCTTACAGAAAAATTGTTGCTTTACGGAGGTGCGATCCAGCAAGCAGGGGCTGTTAAA
GCCCGTGGTGAGCAGCGCAAGGTGACCTCCGATTGGATGGAGTTGGAGAAGCAGCGGGGTATATCGATCACCTCCACCGT
TCTGCAGTTTGATTATGCAGATAACACAATTAACCTTCTTGATACCCCCGGACACCAGGATTTTTCCGAAGACACTTATC
GCACCCTGGCGGCTGCAGATAATGCAGTGATGTTGGAGGATGCAGCTAAGGGTTTAGAACCTCAGACCAGAAAATTGTTT
GAGGTATGTCGCATGAGAAACATTCCAATCTTTACTTTCATAAACAAGATGGATCGTGCAGGGCGGGATCCTCTGTTGCT
GTTGGATGAGATTGAAGCAGAGTTGGAGCTAATACCTTGGGCCGTGAATTGGCCTATTGGTAGTGGGGAGTTGTTTCGTG
GTGTTATTGATCGTCGAACTAGAAATGTTGTTCTTTTTACAAGAGCGGAAAGGGGCCGGCAAGCCAGTGAACGCCACCTT
CATCTTGACGATCCGGAATTGCCAAGCCTTGTCGAGCAGGAGCTTTTGGACCAAGCTTTGGAAGAGTTAGAGCTCTTGGA
AGCAGCAGGTGCAGAGCTTGATCTGGAATTAGTCCATGCAGGCGAGTTGACACCTGTGTTTTTTGGTTCAGCAATGACCA
ATTTTGGGGTAAGGCCTTTCCTTGATGCCTTTCTTGAGATGGCGCAGCGTCCGGTAGCTCGTTCTACAAGTGAGGGCCCT
GTTGATCCTCTGCGACCATCCTTTAGTGGCTTTGTTTTCAAATTACAGGCCAATATGGATCCTCGCCATCGTGATCGTGT
CGCCTTTGTAAGGGTCTGCAGCGGACGTTTTGAAAAGGATATGACGGTGCGCCTAGCACGAACCGGTAAATCCATACGTC
TATCTCGCCCACAGAAGTTATTTGGCCAAGAACGTTCGGTCGTTGATGATGCCTATCCAGGTGATGTTATTGGTCTAAAT
AATCCTGGTATGTTTGCGATTGGTGATACTCTGTATACAGGATCTAGAGTTGAATACGAGGGTATACCATGCTTTAGTCC
TGAGATATTTAGCTGGTTACGTAACCCGAATCCTTCAGCATTTAAAAATTTTCGTAAAGGAGTTAATGAGTTAAGAGAGG
AAGGTGCTGTACAAATTCTCTACGATACTGATCAGAGCAAGCGGGATCCGATTCTTGCTGCAGTAGGTCAGTTGCAGCTT
GATGTTGTTCAGCATCGTTTAGAAAACGAATATGGCGTTCAGACCCGTCTTGAGCCTATGGGCTTCCAGGTGGCACGGTG
GGTCAGTGATGGTTGGCCTGCTCTAGATGAGATGGGACGGATCTTTAATTGCAAGACCGTTCAGGATGCCTGGCAGCGTC
CTGTTCTCTTGTTCAAGAACGAGTGGAACCTTAACCAGCTTCTTGATGATCACCCTGCGTTGGAACTTAGTGCTGTTGCG
CCTGTGGTTAGTGGTGTAGAGCCGATCAGCCTTTGA

Upstream 100 bases:

>100_bases
AGAGGCTGAAATATCACCTTTACACCTGCCGCGAAGTGAATTCCTTGTCTTATGAATCTTAGACCCAGTCAAAGGCTTGA
TCATTTATCGCACCTTTAAT

Downstream 100 bases:

>100_bases
TCGTTGGGCTTAAATCTGCTTTCCTGACAAAAAGAACTCGCGATTCACTGGTCTAGTTAGCCAGAATCGTTAAAGAATGT
TTTCAGTGGTAGTGGACAAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MHLHPPDLRHSLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVK
ARGEQRKVTSDWMELEKQRGISITSTVLQFDYADNTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLF
EVCRMRNIPIFTFINKMDRAGRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPFLDAFLEMAQRPVARSTSEGP
VDPLRPSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKDMTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLN
NPGMFAIGDTLYTGSRVEYEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKNEWNLNQLLDDHPALELSAVA
PVVSGVEPISL

Sequences:

>Translated_571_residues
MHLHPPDLRHSLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVK
ARGEQRKVTSDWMELEKQRGISITSTVLQFDYADNTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLF
EVCRMRNIPIFTFINKMDRAGRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPFLDAFLEMAQRPVARSTSEGP
VDPLRPSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKDMTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLN
NPGMFAIGDTLYTGSRVEYEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKNEWNLNQLLDDHPALELSAVA
PVVSGVEPISL
>Mature_571_residues
MHLHPPDLRHSLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVK
ARGEQRKVTSDWMELEKQRGISITSTVLQFDYADNTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLF
EVCRMRNIPIFTFINKMDRAGRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPFLDAFLEMAQRPVARSTSEGP
VDPLRPSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKDMTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLN
NPGMFAIGDTLYTGSRVEYEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKNEWNLNQLLDDHPALELSAVA
PVVSGVEPISL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=477, Percent_Identity=30.398322851153, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI19923640, Length=572, Percent_Identity=26.9230769230769, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI25306287, Length=544, Percent_Identity=27.2058823529412, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI25306283, Length=432, Percent_Identity=29.8611111111111, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=30.9677419354839, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=35.036496350365, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=166, Percent_Identity=31.9277108433735, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=36.2068965517241, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=36.2068965517241, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=36.2068965517241, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790835, Length=470, Percent_Identity=50.8510638297872, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1789738, Length=488, Percent_Identity=28.8934426229508, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=37.5838926174497, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=32.7868852459016, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789737, Length=137, Percent_Identity=31.3868613138686, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=137, Percent_Identity=31.3868613138686, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=490, Percent_Identity=26.9387755102041, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=484, Percent_Identity=25.6198347107438, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=34, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=32.0987654320988, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=150, Percent_Identity=31.3333333333333, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=150, Percent_Identity=31.3333333333333, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=483, Percent_Identity=29.6066252587992, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6322359, Length=510, Percent_Identity=26.078431372549, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6323320, Length=172, Percent_Identity=31.9767441860465, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=32.7044025157233, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=32.7044025157233, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582462, Length=489, Percent_Identity=30.879345603272, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI221458488, Length=524, Percent_Identity=26.3358778625954, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=34.375, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=182, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585711, Length=182, Percent_Identity=31.3186813186813, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585713, Length=182, Percent_Identity=31.3186813186813, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574573, Length=159, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 64050; Mature: 64050

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00732 RIBOSOMAL_S16 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLHPPDLRHSLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKT
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
TLTEKLLLYGGAIQQAGAVKARGEQRKVTSDWMELEKQRGISITSTVLQFDYADNTINLL
HHHHHHHHHCCHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCEEEEHHEEEECCCCEEEEE
DTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRNIPIFTFINKMDRA
ECCCCCCCCHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
CCCCEEEHHHHCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHH
LDAFLEMAQRPVARSTSEGPVDPLRPSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKD
HHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
MTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLNNPGMFAIGDTLYTGSRVEYE
CEEEEECCCCCEECCCCHHHHCCCHHHHCCCCCCCEEECCCCCCEEECCEEECCCEEEEC
GIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKN
HHHHHHHCCCCCCCEECCCCCHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHCCCEEEEEC
EWNLNQLLDDHPALELSAVAPVVSGVEPISL
CCCHHHHHCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHLHPPDLRHSLTSLPDSTSPPIDSASSIDIDQVLEASLAEAISRRRNFAIISHPDAGKT
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
TLTEKLLLYGGAIQQAGAVKARGEQRKVTSDWMELEKQRGISITSTVLQFDYADNTINLL
HHHHHHHHHCCHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCEEEEHHEEEECCCCEEEEE
DTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRNIPIFTFINKMDRA
ECCCCCCCCHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GRDPLLLLDEIEAELELIPWAVNWPIGSGELFRGVIDRRTRNVVLFTRAERGRQASERHL
CCCCEEEHHHHCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCC
HLDDPELPSLVEQELLDQALEELELLEAAGAELDLELVHAGELTPVFFGSAMTNFGVRPF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHH
LDAFLEMAQRPVARSTSEGPVDPLRPSFSGFVFKLQANMDPRHRDRVAFVRVCSGRFEKD
HHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCC
MTVRLARTGKSIRLSRPQKLFGQERSVVDDAYPGDVIGLNNPGMFAIGDTLYTGSRVEYE
CEEEEECCCCCEECCCCHHHHCCCHHHHCCCCCCCEEECCCCCCEEECCEEECCCEEEEC
GIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDTDQSKRDPILAAVGQLQL
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
DVVQHRLENEYGVQTRLEPMGFQVARWVSDGWPALDEMGRIFNCKTVQDAWQRPVLLFKN
HHHHHHHCCCCCCCEECCCCCHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHCCCEEEEEC
EWNLNQLLDDHPALELSAVAPVVSGVEPISL
CCCHHHHHCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA