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The map label for this gene is purN [H]

Identifier: 33862982

GI number: 33862982

Start: 766458

End: 767180

Strand: Reverse

Name: purN [H]

Synonym: PMT0710

Alternate gene names: 33862982

Gene position: 767180-766458 (Counterclockwise)

Preceding gene: 33862985

Following gene: 33862980

Centisome position: 31.82

GC content: 48.13

Gene sequence:

>723_bases
ATGAACAACTTAGATAAAAAGGAGATTTGTCTGACCAGTGCTGCTTCGAATCCTCTTCAGAACAGTTGCTATGAAAAGGA
TGCGAAGCGATCGCTGATCTGGCCACCCCTCCAGGCCAGCCCAAAATTCAACCCTCGCTTAAATCTTGGCGTGATGGCAT
CAGGGAATGGCAGCAATTTTGAAGCCCTGGTTAAAGCCATTCAAAATTCTCAACTTGATGCTTATATTTCCATATTGGTG
GTTAACAACCCTAATTGTGAAGCAAGTCTCCGGGCCAAACGGCTAGGTGTGCCTTGTGTCATCCACGATCATCGCGAATT
CAGTAGTCGAGAAGAACTGGATAAGGCCTTGGTAAAGACATTCACTAACCATGCTGTAGAAGGCGTGGTGATGGCCGGCT
GGATGCGAATCGTCACCCCAATCCTGATTGCTGCTTTCCCGAATCGCCTTATTAATATTCACCCATCTCTGCTTCCTAGC
TTTCGCGGTTTAGATGCGGTTGGGCAAGCACTTAAAGCGAGAGTTGCTATCAGTGGTTGTTCCGTTCACCTGGTTACACC
TCAAGTAGATGACGGTCCGGTGCTTGCGCAAGCTGCAGTGCCAGTTTTGAGCTCAGACGACCATCAGAGCCTCAGCAAAA
GGATCCAGCGCATGGAGCATCAACTGCTTCCATTATCTGTTGCTCTTGCAGGCCAGAATTGGAGAAACGCAGCTCAAAAT
TAA

Upstream 100 bases:

>100_bases
GGATCACAGCAGGGGTTCCAATTGGATGCTGCACATAGGAATTAAGTTGTGAATTGGATGTGCTTAGAGCATGTAGCTGG
TCTTCTCAATCGTCAACAAG

Downstream 100 bases:

>100_bases
GGGTAGAAGGTCAGTAAAGACATCCCTGTGGTTGGTGGTAATCCAGCCATCAGGTTTAGACATTGAACCCCTTGACCAGC
TTGGCCTTTAATCAGGTTGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNNLDKKEICLTSAASNPLQNSCYEKDAKRSLIWPPLQASPKFNPRLNLGVMASGNGSNFEALVKAIQNSQLDAYISILV
VNNPNCEASLRAKRLGVPCVIHDHREFSSREELDKALVKTFTNHAVEGVVMAGWMRIVTPILIAAFPNRLINIHPSLLPS
FRGLDAVGQALKARVAISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSKRIQRMEHQLLPLSVALAGQNWRNAAQN

Sequences:

>Translated_240_residues
MNNLDKKEICLTSAASNPLQNSCYEKDAKRSLIWPPLQASPKFNPRLNLGVMASGNGSNFEALVKAIQNSQLDAYISILV
VNNPNCEASLRAKRLGVPCVIHDHREFSSREELDKALVKTFTNHAVEGVVMAGWMRIVTPILIAAFPNRLINIHPSLLPS
FRGLDAVGQALKARVAISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSKRIQRMEHQLLPLSVALAGQNWRNAAQN
>Mature_240_residues
MNNLDKKEICLTSAASNPLQNSCYEKDAKRSLIWPPLQASPKFNPRLNLGVMASGNGSNFEALVKAIQNSQLDAYISILV
VNNPNCEASLRAKRLGVPCVIHDHREFSSREELDKALVKTFTNHAVEGVVMAGWMRIVTPILIAAFPNRLINIHPSLLPS
FRGLDAVGQALKARVAISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSKRIQRMEHQLLPLSVALAGQNWRNAAQN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-31,
Organism=Escherichia coli, GI1788846, Length=182, Percent_Identity=36.2637362637363, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787483, Length=138, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=32.9608938547486, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=37.6344086021505, Blast_Score=138, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 26151; Mature: 26151

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLDKKEICLTSAASNPLQNSCYEKDAKRSLIWPPLQASPKFNPRLNLGVMASGNGSNF
CCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCH
EALVKAIQNSQLDAYISILVVNNPNCEASLRAKRLGVPCVIHDHREFSSREELDKALVKT
HHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHH
FTNHAVEGVVMAGWMRIVTPILIAAFPNRLINIHPSLLPSFRGLDAVGQALKARVAISGC
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCHHHCCCCCCHHHHHHHHHHHHEEECC
SVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSKRIQRMEHQLLPLSVALAGQNWRNAAQN
EEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHCCCC
>Mature Secondary Structure
MNNLDKKEICLTSAASNPLQNSCYEKDAKRSLIWPPLQASPKFNPRLNLGVMASGNGSNF
CCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCH
EALVKAIQNSQLDAYISILVVNNPNCEASLRAKRLGVPCVIHDHREFSSREELDKALVKT
HHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHH
FTNHAVEGVVMAGWMRIVTPILIAAFPNRLINIHPSLLPSFRGLDAVGQALKARVAISGC
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCHHHCCCCCCHHHHHHHHHHHHEEECC
SVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSKRIQRMEHQLLPLSVALAGQNWRNAAQN
EEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]