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The map label for this gene is hisZ [H]

Identifier: 33862970

GI number: 33862970

Start: 752902

End: 754080

Strand: Reverse

Name: hisZ [H]

Synonym: PMT0698

Alternate gene names: 33862970

Gene position: 754080-752902 (Counterclockwise)

Preceding gene: 33862971

Following gene: 33862969

Centisome position: 31.28

GC content: 52.25

Gene sequence:

>1179_bases
ATGGCCCTGCAACCGGCAGCGGGTGCTCGCGATCTGAACCCCCAGCAGGTCGAGCTAAACCAAAAACTTAGCCAGCGACT
TGCCGAGGTTTATCGACTATGGGGCTACGACGAAGTCTCTCCTCCAAGGGTCGAGCGACTCGAAACCTTGAAAGCTGGTG
GTGCTATTGCCAGTCAGGACATCGTGCGATTGGTGGCCGATGAACCCCTTGGTCTACGACCAGAGATGACAGCATCAATC
GCTCGCGCCGCTTGTACAAGGCTTAAACAAAGACCTCGTCCCTTGAGGCTATGGGCTGCAGGAACAATTTTTGAAAGCCG
TACTGCTGATGAAGGAAGCCTTTGCATCGAAGAAAACCTTCAAAGCGGTGTAGAGCTCTTCGGGGTCGAGCCCATCAATG
CGGAAATGGAGCTTCTTTCCCTGCTGTTTTCAGCAGTGGAGACTCTCGAACTGAGTAAACGACACCAGCCACGTTTACTC
GTTGGCCACACAGCATTAATGGATCTCATCATGCTGCCATTTCAAAACGATCTACGCGAAAAAATAAGAACAGCATTGAT
CCACTATGACCGCCTTGCTCTTGAAAGCCTGCAACTGCCGAATGATCAATTCGAGCGCCTCCTCCATCACTTGGAGTGCC
GTGGTGAACCACTAGACGTGCTCGAACGCTTATCAGGCCTATTTGGAACTCAACAAGCACTCAATAACCTACAAAGGCTA
TTTGAACAGATGAGTCCACTGGCCGCTGATCAGGGGATTGACCTGCAACTGGATCCAACCTTTCAGCCCCATTTTGAGCT
CTACACCGGGCTGGTGTTTCAGTTGGTATGTCAAAGCGATGCTGCACCTGTCGTGATTGCTCGTGGGGGGCGTTACGACA
ACCTTGTAGCTCGTTGCGGTGCCAAAGGGCTTCAAGCAGCAGGGGTAGGCTTTAGTTTCGCCATAGACGATATTCGTGAA
CTGCTGACCAAAGAGATCAAAGCGTCTGACGCTGTGGAAACAACCCTTGTTGCCTACGGCGAGCAAGCAACGCTGGAACA
TGCACTCAAGCGTCAGCGTCACTGGCACAAGCAGGGCCAGCGTGCTGTTGTTGAACTTGAAGCCTGTCGCGATCGTGAAG
ATGCCTTCAGCCGTCTATCAGATCGGGGCTGTAGCACACTGGACTGGTTAGATCATTAG

Upstream 100 bases:

>100_bases
GTTGATCAACTCAGCAAAGTAAAACCCCTTGAAGCGGATTCATTTGGCGCTCCCGAACTCAGGAGCATGGGATCCTGAAG
TCACTCCGAGAAAATCAGGG

Downstream 100 bases:

>100_bases
GATTTCGAGAGTCTCGTCGTCCGATCTGCAAGGCCATGCCACACAGCATCGTGACAGAAATATGTGAAGGGGTTGCTGAC
TGTGCTCAGGCATGCCCTGT

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MALQPAAGARDLNPQQVELNQKLSQRLAEVYRLWGYDEVSPPRVERLETLKAGGAIASQDIVRLVADEPLGLRPEMTASI
ARAACTRLKQRPRPLRLWAAGTIFESRTADEGSLCIEENLQSGVELFGVEPINAEMELLSLLFSAVETLELSKRHQPRLL
VGHTALMDLIMLPFQNDLREKIRTALIHYDRLALESLQLPNDQFERLLHHLECRGEPLDVLERLSGLFGTQQALNNLQRL
FEQMSPLAADQGIDLQLDPTFQPHFELYTGLVFQLVCQSDAAPVVIARGGRYDNLVARCGAKGLQAAGVGFSFAIDDIRE
LLTKEIKASDAVETTLVAYGEQATLEHALKRQRHWHKQGQRAVVELEACRDREDAFSRLSDRGCSTLDWLDH

Sequences:

>Translated_392_residues
MALQPAAGARDLNPQQVELNQKLSQRLAEVYRLWGYDEVSPPRVERLETLKAGGAIASQDIVRLVADEPLGLRPEMTASI
ARAACTRLKQRPRPLRLWAAGTIFESRTADEGSLCIEENLQSGVELFGVEPINAEMELLSLLFSAVETLELSKRHQPRLL
VGHTALMDLIMLPFQNDLREKIRTALIHYDRLALESLQLPNDQFERLLHHLECRGEPLDVLERLSGLFGTQQALNNLQRL
FEQMSPLAADQGIDLQLDPTFQPHFELYTGLVFQLVCQSDAAPVVIARGGRYDNLVARCGAKGLQAAGVGFSFAIDDIRE
LLTKEIKASDAVETTLVAYGEQATLEHALKRQRHWHKQGQRAVVELEACRDREDAFSRLSDRGCSTLDWLDH
>Mature_391_residues
ALQPAAGARDLNPQQVELNQKLSQRLAEVYRLWGYDEVSPPRVERLETLKAGGAIASQDIVRLVADEPLGLRPEMTASIA
RAACTRLKQRPRPLRLWAAGTIFESRTADEGSLCIEENLQSGVELFGVEPINAEMELLSLLFSAVETLELSKRHQPRLLV
GHTALMDLIMLPFQNDLREKIRTALIHYDRLALESLQLPNDQFERLLHHLECRGEPLDVLERLSGLFGTQQALNNLQRLF
EQMSPLAADQGIDLQLDPTFQPHFELYTGLVFQLVCQSDAAPVVIARGGRYDNLVARCGAKGLQAAGVGFSFAIDDIREL
LTKEIKASDAVETTLVAYGEQATLEHALKRQRHWHKQGQRAVVELEACRDREDAFSRLSDRGCSTLDWLDH

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 43943; Mature: 43812

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQPAAGARDLNPQQVELNQKLSQRLAEVYRLWGYDEVSPPRVERLETLKAGGAIASQD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
IVRLVADEPLGLRPEMTASIARAACTRLKQRPRPLRLWAAGTIFESRTADEGSLCIEENL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCCCHHHHHHH
QSGVELFGVEPINAEMELLSLLFSAVETLELSKRHQPRLLVGHTALMDLIMLPFQNDLRE
HCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCHHHHHH
KIRTALIHYDRLALESLQLPNDQFERLLHHLECRGEPLDVLERLSGLFGTQQALNNLQRL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FEQMSPLAADQGIDLQLDPTFQPHFELYTGLVFQLVCQSDAAPVVIARGGRYDNLVARCG
HHHHCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHC
AKGLQAAGVGFSFAIDDIRELLTKEIKASDAVETTLVAYGEQATLEHALKRQRHWHKQGQ
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
RAVVELEACRDREDAFSRLSDRGCSTLDWLDH
HHEEHHHHHCCHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
ALQPAAGARDLNPQQVELNQKLSQRLAEVYRLWGYDEVSPPRVERLETLKAGGAIASQD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHH
IVRLVADEPLGLRPEMTASIARAACTRLKQRPRPLRLWAAGTIFESRTADEGSLCIEENL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCCCHHHHHHH
QSGVELFGVEPINAEMELLSLLFSAVETLELSKRHQPRLLVGHTALMDLIMLPFQNDLRE
HCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCHHHHHH
KIRTALIHYDRLALESLQLPNDQFERLLHHLECRGEPLDVLERLSGLFGTQQALNNLQRL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FEQMSPLAADQGIDLQLDPTFQPHFELYTGLVFQLVCQSDAAPVVIARGGRYDNLVARCG
HHHHCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHC
AKGLQAAGVGFSFAIDDIRELLTKEIKASDAVETTLVAYGEQATLEHALKRQRHWHKQGQ
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
RAVVELEACRDREDAFSRLSDRGCSTLDWLDH
HHEEHHHHHCCHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA