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The map label for this gene is ubiE [H]

Identifier: 33862885

GI number: 33862885

Start: 666715

End: 667788

Strand: Reverse

Name: ubiE [H]

Synonym: PMT0612

Alternate gene names: 33862885

Gene position: 667788-666715 (Counterclockwise)

Preceding gene: 33862886

Following gene: 33862884

Centisome position: 27.7

GC content: 53.07

Gene sequence:

>1074_bases
ATGGCGCAGCCAAGCCTCACCAAGTTCGCTTATCAAACCCTGCAGCAGAGCAAGACTCTTGCGGGCCTGGCCCACAAGGA
ATTGAGCACCAAGCTGATGGAATTGCTGGCACCTGATGCGATGCCAAGCATTGAACCGATTCCGCGAGAATTGCTGATGG
AGCTGCGCAGCTCAATGTCAGCTCTCGAGCAACTTGACTGGCATGAAGCTGAACAAGGCATTTATCCAAAAACGCAGCTC
TTTGAAGCTCCGTGGTTGGAATGGGCGAGCCGTTACCCCTTGGTCTGGTTAGATCTTCCCTCGACATGGCAGCGGCGCAA
GGCACGCAATATTCATGATCTGCCAAACGATGTCGATTCAGAGATCTATCCCGATTACTACCTGCAAAACTTTCATCACC
AAACCGATGGCTATCTAAGCGACCACTCTGCAGAACTTTATGACCTGCAGGTAGAGATTCTCTTTAATGGCACTGCAGAC
TCCATGCGGCGCAGGGTTTTAGCGCCTCTCAAGCGCGGGCTGAAACGCTTCCAAAGCCGCAGCTCCGCCAGTCTTCGGGT
GTTGGATGTCGCAACCGGAACCGGTCGAACCCTGCAACAGATTCGCAGCGCCCTACCCAAAGTTGAACTGCTGGGGTTGG
ATCTATCAGCAGCTTATTTACGTCAGGCCAGCCGCTCGCTCAACAGCCGCAATGGAGAACTCGCGCAACTGGTGCGCGGT
AATGCTGAACAGCTGCCGTTTGCTGATCAAAGTGTGCAAGGCATCACGTGCGTTTTCCTGTTTCACGAATTACCGAGCTC
GGCCCGGCAAAATGTTCTTGACGAATGCTGGCGCGTGCTCGAACCAGGCGGAGTTTTGGTCCTAGCAGATTCTGTACAAA
TGGAAGACTCGCCCAAGTTCATCCCTGCCATGGAGAACTTCCGGCGGATATTTCATGAGCCGTACTACCGCGATTACATC
TCCGACAACATTGAGGCACGTTTCCAAGCAAGCGGTTTTGAGGGCATTGCTGCCGAGTCTCACTTCATGACACGAGTTTG
GACTGCTTTCAAACCGCTCCAAAACAACGCTTAA

Upstream 100 bases:

>100_bases
ATTGTCATAGATCCTTAATTGAATAGGCAAGGCTTATAATCTGAGATCTCAGCAATTGCAAGGCTGATCCGCTGACATAG
AGCACACCCAAATCAGGTTC

Downstream 100 bases:

>100_bases
GCGGGTTTCCTGACACTCGGTAGTTCAATCGAGTCTCACAAATAGGGTGCTCTTGTTAGATGCAAAATCCGGTGTATTTT
TTCCTGAAGGGACAACGTGA

Product: methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MAQPSLTKFAYQTLQQSKTLAGLAHKELSTKLMELLAPDAMPSIEPIPRELLMELRSSMSALEQLDWHEAEQGIYPKTQL
FEAPWLEWASRYPLVWLDLPSTWQRRKARNIHDLPNDVDSEIYPDYYLQNFHHQTDGYLSDHSAELYDLQVEILFNGTAD
SMRRRVLAPLKRGLKRFQSRSSASLRVLDVATGTGRTLQQIRSALPKVELLGLDLSAAYLRQASRSLNSRNGELAQLVRG
NAEQLPFADQSVQGITCVFLFHELPSSARQNVLDECWRVLEPGGVLVLADSVQMEDSPKFIPAMENFRRIFHEPYYRDYI
SDNIEARFQASGFEGIAAESHFMTRVWTAFKPLQNNA

Sequences:

>Translated_357_residues
MAQPSLTKFAYQTLQQSKTLAGLAHKELSTKLMELLAPDAMPSIEPIPRELLMELRSSMSALEQLDWHEAEQGIYPKTQL
FEAPWLEWASRYPLVWLDLPSTWQRRKARNIHDLPNDVDSEIYPDYYLQNFHHQTDGYLSDHSAELYDLQVEILFNGTAD
SMRRRVLAPLKRGLKRFQSRSSASLRVLDVATGTGRTLQQIRSALPKVELLGLDLSAAYLRQASRSLNSRNGELAQLVRG
NAEQLPFADQSVQGITCVFLFHELPSSARQNVLDECWRVLEPGGVLVLADSVQMEDSPKFIPAMENFRRIFHEPYYRDYI
SDNIEARFQASGFEGIAAESHFMTRVWTAFKPLQNNA
>Mature_356_residues
AQPSLTKFAYQTLQQSKTLAGLAHKELSTKLMELLAPDAMPSIEPIPRELLMELRSSMSALEQLDWHEAEQGIYPKTQLF
EAPWLEWASRYPLVWLDLPSTWQRRKARNIHDLPNDVDSEIYPDYYLQNFHHQTDGYLSDHSAELYDLQVEILFNGTADS
MRRRVLAPLKRGLKRFQSRSSASLRVLDVATGTGRTLQQIRSALPKVELLGLDLSAAYLRQASRSLNSRNGELAQLVRGN
AEQLPFADQSVQGITCVFLFHELPSSARQNVLDECWRVLEPGGVLVLADSVQMEDSPKFIPAMENFRRIFHEPYYRDYIS
DNIEARFQASGFEGIAAESHFMTRVWTAFKPLQNNA

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 40787; Mature: 40656

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPSLTKFAYQTLQQSKTLAGLAHKELSTKLMELLAPDAMPSIEPIPRELLMELRSSMS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALEQLDWHEAEQGIYPKTQLFEAPWLEWASRYPLVWLDLPSTWQRRKARNIHDLPNDVDS
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCCC
EIYPDYYLQNFHHQTDGYLSDHSAELYDLQVEILFNGTADSMRRRVLAPLKRGLKRFQSR
CCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
SSASLRVLDVATGTGRTLQQIRSALPKVELLGLDLSAAYLRQASRSLNSRNGELAQLVRG
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHCC
NAEQLPFADQSVQGITCVFLFHELPSSARQNVLDECWRVLEPGGVLVLADSVQMEDSPKF
CHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
IPAMENFRRIFHEPYYRDYISDNIEARFQASGFEGIAAESHFMTRVWTAFKPLQNNA
CHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQPSLTKFAYQTLQQSKTLAGLAHKELSTKLMELLAPDAMPSIEPIPRELLMELRSSMS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALEQLDWHEAEQGIYPKTQLFEAPWLEWASRYPLVWLDLPSTWQRRKARNIHDLPNDVDS
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCCC
EIYPDYYLQNFHHQTDGYLSDHSAELYDLQVEILFNGTADSMRRRVLAPLKRGLKRFQSR
CCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
SSASLRVLDVATGTGRTLQQIRSALPKVELLGLDLSAAYLRQASRSLNSRNGELAQLVRG
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHCC
NAEQLPFADQSVQGITCVFLFHELPSSARQNVLDECWRVLEPGGVLVLADSVQMEDSPKF
CHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
IPAMENFRRIFHEPYYRDYISDNIEARFQASGFEGIAAESHFMTRVWTAFKPLQNNA
CHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]