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The map label for this gene is pyrC [H]

Identifier: 33862741

GI number: 33862741

Start: 518998

End: 520062

Strand: Reverse

Name: pyrC [H]

Synonym: PMT0468

Alternate gene names: 33862741

Gene position: 520062-518998 (Counterclockwise)

Preceding gene: 33862743

Following gene: 33862740

Centisome position: 21.57

GC content: 49.2

Gene sequence:

>1065_bases
TTGGATTCTTTGCCCGACCAACTCATTTTGCGTCAACCGGATGACTGGCATGTCCATCTGAGGGATGGTGCCATGCTGCA
TGCGGTGCTAGGCAGCACAGCTCGTGTGTTTCGACGAGCAATCGTGATGCCCAATCTCAGGCCACCAATCACGAGTGTAG
AAGCAGCAAAAACCTATCGCGACCAGATTTTGGCAGCCCTTCCTGATGGTGTTCCGTTTACGCCATTGATGACGGCCTAC
CTCAACGAAAGCTTGGCTCCAGATGTTTTAGAACAAGGGCATCAACAGCATGTTTTCATCGCCGCCAAGCTTTATCCAGC
CCATGCCACAACTAATTCCGAGCAGGGGGTCAGTGATCTAAGAGCGATTAACTCACTTCTAGAGACGATGGAGAAAATTG
GGATGCCATTGCTCGTTCATGGTGAGGTGAGCGATGTCGATATCGACATTTTTGATAGAGAAGCCTTTTTTATTGAGCAC
CACCTGGCACCACTAATAGGGCGTTATCCAAATCTTCGTGTCGTGATGGAGCACATCACGACGCAAGAGGCAGTTCAGTT
CGTGGAAACAGGTGGGCCGAATCTGGCAGCCACTATCACTCCTCATCATTTACATATCAACCGTAATGCAATGTTTCTCG
GTGGTTTTCGTAGTGATTTCTACTGCTTACCAGTAGCCAAGCGTGAACGCCATCGACTCTCCCTGCGTCGAGCAGCGACA
AGTGGCAAACCATGCTTTTTCCTTGGCACTGATTCGGCACCACACCCTCGCTCTGCAAAGGAGAGTGCTTGTAGTTGTGG
TGGCATTTTCAATGCTCATTATGCGATGGAAAGTTATGCCGAAGTCTTTGAGCAGGAAGGAGCACTTGATCGCCTTGAGG
CCTTCTCCAGTGAATACGGTCCAGCTTTCTATGGCTTGCCCCTCAATAACACCTCAATCAAACTGATACGAAGAGCTCAT
GTTGTACCAGCTACTTTTAGCGGACAAACGAACGCAGATTCTTCTGAACACTTAGTTCCATTTCATGCTGGCGAACTGTT
GGGCTGGTCTGTTTCGGTTGATTGA

Upstream 100 bases:

>100_bases
ATCTTTGGGAGCACTTCCAGAAAGGGTTTATCACAGCAAGTTTATTTTTGGGTCTTACTTCGGTGCTGAAATCTCTACAC
CTCATTCTGGGTTGATGACT

Downstream 100 bases:

>100_bases
TAAAGCACGCATTGGAGCGCATGAATTGCACCATTGCTCGAGCTGGCAATCAAGGATCCGATTGGGGTCTGGCTAGCAAC
TTGAATCTCTACGCAAATGG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MDSLPDQLILRQPDDWHVHLRDGAMLHAVLGSTARVFRRAIVMPNLRPPITSVEAAKTYRDQILAALPDGVPFTPLMTAY
LNESLAPDVLEQGHQQHVFIAAKLYPAHATTNSEQGVSDLRAINSLLETMEKIGMPLLVHGEVSDVDIDIFDREAFFIEH
HLAPLIGRYPNLRVVMEHITTQEAVQFVETGGPNLAATITPHHLHINRNAMFLGGFRSDFYCLPVAKRERHRLSLRRAAT
SGKPCFFLGTDSAPHPRSAKESACSCGGIFNAHYAMESYAEVFEQEGALDRLEAFSSEYGPAFYGLPLNNTSIKLIRRAH
VVPATFSGQTNADSSEHLVPFHAGELLGWSVSVD

Sequences:

>Translated_354_residues
MDSLPDQLILRQPDDWHVHLRDGAMLHAVLGSTARVFRRAIVMPNLRPPITSVEAAKTYRDQILAALPDGVPFTPLMTAY
LNESLAPDVLEQGHQQHVFIAAKLYPAHATTNSEQGVSDLRAINSLLETMEKIGMPLLVHGEVSDVDIDIFDREAFFIEH
HLAPLIGRYPNLRVVMEHITTQEAVQFVETGGPNLAATITPHHLHINRNAMFLGGFRSDFYCLPVAKRERHRLSLRRAAT
SGKPCFFLGTDSAPHPRSAKESACSCGGIFNAHYAMESYAEVFEQEGALDRLEAFSSEYGPAFYGLPLNNTSIKLIRRAH
VVPATFSGQTNADSSEHLVPFHAGELLGWSVSVD
>Mature_354_residues
MDSLPDQLILRQPDDWHVHLRDGAMLHAVLGSTARVFRRAIVMPNLRPPITSVEAAKTYRDQILAALPDGVPFTPLMTAY
LNESLAPDVLEQGHQQHVFIAAKLYPAHATTNSEQGVSDLRAINSLLETMEKIGMPLLVHGEVSDVDIDIFDREAFFIEH
HLAPLIGRYPNLRVVMEHITTQEAVQFVETGGPNLAATITPHHLHINRNAMFLGGFRSDFYCLPVAKRERHRLSLRRAAT
SGKPCFFLGTDSAPHPRSAKESACSCGGIFNAHYAMESYAEVFEQEGALDRLEAFSSEYGPAFYGLPLNNTSIKLIRRAH
VVPATFSGQTNADSSEHLVPFHAGELLGWSVSVD

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=349, Percent_Identity=56.1604584527221, Blast_Score=381, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323452, Length=362, Percent_Identity=34.2541436464088, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 39151; Mature: 39151

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLPDQLILRQPDDWHVHLRDGAMLHAVLGSTARVFRRAIVMPNLRPPITSVEAAKTYR
CCCCCCHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DQILAALPDGVPFTPLMTAYLNESLAPDVLEQGHQQHVFIAAKLYPAHATTNSEQGVSDL
HHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHH
RAINSLLETMEKIGMPLLVHGEVSDVDIDIFDREAFFIEHHLAPLIGRYPNLRVVMEHIT
HHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHH
TQEAVQFVETGGPNLAATITPHHLHINRNAMFLGGFRSDFYCLPVAKRERHRLSLRRAAT
HHHHHHHHHCCCCCEEEEECCEEEEECCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHC
SGKPCFFLGTDSAPHPRSAKESACSCGGIFNAHYAMESYAEVFEQEGALDRLEAFSSEYG
CCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PAFYGLPLNNTSIKLIRRAHVVPATFSGQTNADSSEHLVPFHAGELLGWSVSVD
CEEEECCCCCCHHEEEEHHEECCEEECCCCCCCCCCCEEEEECCCEEEEEEECC
>Mature Secondary Structure
MDSLPDQLILRQPDDWHVHLRDGAMLHAVLGSTARVFRRAIVMPNLRPPITSVEAAKTYR
CCCCCCHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DQILAALPDGVPFTPLMTAYLNESLAPDVLEQGHQQHVFIAAKLYPAHATTNSEQGVSDL
HHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHH
RAINSLLETMEKIGMPLLVHGEVSDVDIDIFDREAFFIEHHLAPLIGRYPNLRVVMEHIT
HHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHH
TQEAVQFVETGGPNLAATITPHHLHINRNAMFLGGFRSDFYCLPVAKRERHRLSLRRAAT
HHHHHHHHHCCCCCEEEEECCEEEEECCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHC
SGKPCFFLGTDSAPHPRSAKESACSCGGIFNAHYAMESYAEVFEQEGALDRLEAFSSEYG
CCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PAFYGLPLNNTSIKLIRRAHVVPATFSGQTNADSSEHLVPFHAGELLGWSVSVD
CEEEECCCCCCHHEEEEHHEECCEEECCCCCCCCCCCEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA