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The map label for this gene is desA [H]

Identifier: 33862522

GI number: 33862522

Start: 278544

End: 279683

Strand: Reverse

Name: desA [H]

Synonym: PMT0249

Alternate gene names: 33862522

Gene position: 279683-278544 (Counterclockwise)

Preceding gene: 33862523

Following gene: 33862521

Centisome position: 11.6

GC content: 43.86

Gene sequence:

>1140_bases
ATGTCTGGCCTCAATGGAAAGGCGCTCAAGGTTTCCAAAGCGGATATCCCTGATCTAGCTGCAATCAAGGCTATTTTGCC
TCATCAATGCTTGAATTGCAGCACCAACACTTCCCTTGCTTATCTAGCGCAATCTCTGACGATACAGGCCATTGTAATTT
CCATAGGGATGCACATCCCTCTAAATCTGGAAATATTACCTGTTTGGGTTTTCTACTGGCTTGTATCTGGCACAACAGCA
ATGGGCTTATGGGTTATCGCACATGAATGCGGACACGGTGCTTTTTCGAAAAACAGGAAACTAGAAACATTCGTTGGCTA
TGTATTGCATTCGATGCTTCTTGTTCCATATTTCTCATGGCAGCGTTCACATTTAGTTCACCATACTTATACAAACCATA
TCGCCAATGGTGAGACTCACGTGCCTTTAGTCATTCGTGGCAACGGAATTGATGAGCAGGCTGGTGGAGAAAAAGATATT
GCCATAGCAGGCAGATTAGGAAAAGTTCAATATGGTGTATTTCAGCTTGTGCTTCATCTGGTTTTTGGCTGGCCAGCCTA
TTTGCTGACTGGGAAGACGGGAGGCCCAAAATATGGCCTATCGAATCACTTCTGGCCGATAGCACCTTTCTCTAAAAAAT
TGTGGACAGAAAAATGGATAAATAAAGTCTGGCTTTCCGATTGGGGGATATGTCTGGCTCTGTTTGGATTGATTGCCTGG
AGCCTACATGATGGCTTTATTACTGTTTTTACAATTTACATAGCCCCTCTGTTAGTAGTCAATATCTGGCTAGTGACCTA
TACGTGGCTGCATCATACTGATACCGATGTTCCCCACCTTAGCAGTTCAGACTTCTCCCAATTGCGAGGTGCGTTCTTAT
CGATTGATAGGCCATATGGAAAAGTAATTGATTTCCTCCATCACAAGATAGGCTCTACTCATGCCATTCATCACATAGCA
CCTTGGATGCCTCATTACCATGCGGGCGAAGCAACTATTGCTCTAAAGAATGCTTTCCCAAAGGTATATCTTTACAGTCA
AACACCAATTCTTCAGGCTCTCTGGCTTATTTCTACTAACTGCATAGCTGTCACTCGGGAAGAGAACAGTGGGCGCTATG
TCTGGAAAAATCCTTGGTGA

Upstream 100 bases:

>100_bases
ATTATATCCAATGAGCCCTTGCCAAGCATAGTCATTGAAAGATCATTCAGAAAGCCATGAAAAAACATTTTTTAGACAAA
ACCCTGCCAAAGGCATCATC

Downstream 100 bases:

>100_bases
GATGACGACTATCGAGGAAGCTATATTAACTGGCTCAATATCTATCTAGTCAATTATTTAATCATCATTTACAACCAATA
CCTATATTCACTCCATTAAA

Product: fatty acid desaturase, type 2

Products: NA

Alternate protein names: Delta(12)-desaturase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSGLNGKALKVSKADIPDLAAIKAILPHQCLNCSTNTSLAYLAQSLTIQAIVISIGMHIPLNLEILPVWVFYWLVSGTTA
MGLWVIAHECGHGAFSKNRKLETFVGYVLHSMLLVPYFSWQRSHLVHHTYTNHIANGETHVPLVIRGNGIDEQAGGEKDI
AIAGRLGKVQYGVFQLVLHLVFGWPAYLLTGKTGGPKYGLSNHFWPIAPFSKKLWTEKWINKVWLSDWGICLALFGLIAW
SLHDGFITVFTIYIAPLLVVNIWLVTYTWLHHTDTDVPHLSSSDFSQLRGAFLSIDRPYGKVIDFLHHKIGSTHAIHHIA
PWMPHYHAGEATIALKNAFPKVYLYSQTPILQALWLISTNCIAVTREENSGRYVWKNPW

Sequences:

>Translated_379_residues
MSGLNGKALKVSKADIPDLAAIKAILPHQCLNCSTNTSLAYLAQSLTIQAIVISIGMHIPLNLEILPVWVFYWLVSGTTA
MGLWVIAHECGHGAFSKNRKLETFVGYVLHSMLLVPYFSWQRSHLVHHTYTNHIANGETHVPLVIRGNGIDEQAGGEKDI
AIAGRLGKVQYGVFQLVLHLVFGWPAYLLTGKTGGPKYGLSNHFWPIAPFSKKLWTEKWINKVWLSDWGICLALFGLIAW
SLHDGFITVFTIYIAPLLVVNIWLVTYTWLHHTDTDVPHLSSSDFSQLRGAFLSIDRPYGKVIDFLHHKIGSTHAIHHIA
PWMPHYHAGEATIALKNAFPKVYLYSQTPILQALWLISTNCIAVTREENSGRYVWKNPW
>Mature_378_residues
SGLNGKALKVSKADIPDLAAIKAILPHQCLNCSTNTSLAYLAQSLTIQAIVISIGMHIPLNLEILPVWVFYWLVSGTTAM
GLWVIAHECGHGAFSKNRKLETFVGYVLHSMLLVPYFSWQRSHLVHHTYTNHIANGETHVPLVIRGNGIDEQAGGEKDIA
IAGRLGKVQYGVFQLVLHLVFGWPAYLLTGKTGGPKYGLSNHFWPIAPFSKKLWTEKWINKVWLSDWGICLALFGLIAWS
LHDGFITVFTIYIAPLLVVNIWLVTYTWLHHTDTDVPHLSSSDFSQLRGAFLSIDRPYGKVIDFLHHKIGSTHAIHHIAP
WMPHYHAGEATIALKNAFPKVYLYSQTPILQALWLISTNCIAVTREENSGRYVWKNPW

Specific function: Can introduce a second cis double bond at the Delta(12) position of fatty acid bound to membranes glycerolipids. This enzyme is involved in chilling tolerance because the phase transition temperature of lipids of cellular membranes depends on the degree o

COG id: COG3239

COG function: function code I; Fatty acid desaturase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fatty acid desaturase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17542730, Length=338, Percent_Identity=29.585798816568, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71999459, Length=327, Percent_Identity=28.1345565749235, Blast_Score=120, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005804 [H]

Pfam domain/function: PF00487 FA_desaturase [H]

EC number: 1.14.99.-

Molecular weight: Translated: 42570; Mature: 42439

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLNGKALKVSKADIPDLAAIKAILPHQCLNCSTNTSLAYLAQSLTIQAIVISIGMHIP
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCC
LNLEILPVWVFYWLVSGTTAMGLWVIAHECGHGAFSKNRKLETFVGYVLHSMLLVPYFSW
CCEEHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QRSHLVHHTYTNHIANGETHVPLVIRGNGIDEQAGGEKDIAIAGRLGKVQYGVFQLVLHL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
VFGWPAYLLTGKTGGPKYGLSNHFWPIAPFSKKLWTEKWINKVWLSDWGICLALFGLIAW
HHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SLHDGFITVFTIYIAPLLVVNIWLVTYTWLHHTDTDVPHLSSSDFSQLRGAFLSIDRPYG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCCCHH
KVIDFLHHKIGSTHAIHHIAPWMPHYHAGEATIALKNAFPKVYLYSQTPILQALWLISTN
HHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHCCC
CIAVTREENSGRYVWKNPW
EEEEEEECCCCEEEECCCC
>Mature Secondary Structure 
SGLNGKALKVSKADIPDLAAIKAILPHQCLNCSTNTSLAYLAQSLTIQAIVISIGMHIP
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCC
LNLEILPVWVFYWLVSGTTAMGLWVIAHECGHGAFSKNRKLETFVGYVLHSMLLVPYFSW
CCEEHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QRSHLVHHTYTNHIANGETHVPLVIRGNGIDEQAGGEKDIAIAGRLGKVQYGVFQLVLHL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
VFGWPAYLLTGKTGGPKYGLSNHFWPIAPFSKKLWTEKWINKVWLSDWGICLALFGLIAW
HHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SLHDGFITVFTIYIAPLLVVNIWLVTYTWLHHTDTDVPHLSSSDFSQLRGAFLSIDRPYG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCCCHH
KVIDFLHHKIGSTHAIHHIAPWMPHYHAGEATIALKNAFPKVYLYSQTPILQALWLISTN
HHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHCCC
CIAVTREENSGRYVWKNPW
EEEEEEECCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA