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The map label for this gene is mltD [C]

Identifier: 33862463

GI number: 33862463

Start: 219569

End: 221014

Strand: Reverse

Name: mltD [C]

Synonym: PMT0190

Alternate gene names: 33862463

Gene position: 221014-219569 (Counterclockwise)

Preceding gene: 33862464

Following gene: 33862460

Centisome position: 9.17

GC content: 54.7

Gene sequence:

>1446_bases
ATGCGCCGGATCATTGTTAGCGCTCTAGCCCTGACAACACTTCTACCGCTGCCATCCTGGGGTGCCAGCGTCACAGTGAG
ACCCGGTGACACCCTTTCGGAAATCGCAGCGCGATATCAGGTATCCCTTCGGGCCTTGATGCGGCTGAACGGCCTAGCCA
ATGCAGACAACCTCTTCATCGGCCAGACCCTCAAACTGCCAGGTAGCGCATCAGGCACAGGCCCGGCTGGAGCAAGCCGT
CACACGGTACGAAGTGGCGAGACCCTCAGCACCATTGCTGTTCGCTACCGAGTGCGCCAGCAGGATCTCATCACCCTCAA
TGGTCTGAGCAATGCCGACAATTTATATATCGGCCAAACGTTGAAATTACCGGGAGGTGCTTCCGGGGCAATCAGAGCTG
GCGCCAGTCGTCACACAGTACGCAGTGGCGAAACCCTCAGCATCATTGCTGCCCGCTACCGAGTTCGCCAACAGGATCTC
GTCGCCCTCAATGGCCTTGCCAATGCAGACAACCTCTTCATTGGCCAGACCCTCAAACTGCCAGGCAGCGCATCAGGCAC
AGTCAGAGCTAGCGCCACTCGTCACACAGTTCGCAGTGGCGAAACCCTCAGCACCATTGCTGTTCGCTACCGAGTGCGCC
AGCAGGATCTCATCACCCTCAATGGCCTTGCCAATGCCAACCATGTCGAACGTGGCCAAACCCTGAAACTGCCCCAAGGG
GCTGTTGTTCCCAAACCCAAAGCAGCAGCAAAGCCCAAACCTGTAGCCATTCAGGCAAATCCAAATGCCACCTCCCATAC
CGTGGCTCGAGGGCAAACATTGAACCAAATCGCTGGGGCTTATCAAATCCCTGTAGCAACCCTGATCAAGATCAATGGGA
TCAACAACCCCAACAAGTTATTAGTCGGCAGCAAACTGTCTCTACGGGTCAAACCATCAACAACAACCCAGCCAAAATCC
ACCACGACAGTTGCCATCAAACCGACTGCAAAAACCACCGTTAAACCAACTGTTAAATCCACCACCAAGCCTGCTGCAAA
ACCACAACCCAAGCCCAAGTCCACCACGACAGTTGCCATCAAACCGACTGCAAAAACCACCGTTAAACCAACTGTTAAAC
CAACCGTTAAGTCCACGAGCAAGCCAGCTGCAAAACCACAACCCAAGCCCAAATCCACCCAGCAAGTTGCTGCCAAGCCT
TCGCAAACGCAATGGCGCACTTACGGTTCACTGCAAGTGGACTGGGCCAATTGGAAGTTCATGGGAGGAAGCTACGTGGC
CCCAACCCTCAACAAAGATGGCAAAGCCCTTTATCTGGCTGTGAACTGCCCAGCGCGCAAGATCAATACCACCGGCACGA
ATGGATCTTGGAAAACTTGGGGCGCTCCACAGCAGCGCTTTGAGCATGATCTCGTCAAGGATCTCTGCAAAGCCAAAGGC
GGCTAA

Upstream 100 bases:

>100_bases
TCAAGCCGCTTTTTGCAAGGGCATCATTTCGGGATGCTTGAAAAGGGCTAGCGCATCTGTGGAGGCAGCTTATCTTTCAC
CAAACTGTAGAGAGAGATTG

Downstream 100 bases:

>100_bases
ATGCATCGAGGGCTGCACTCAGGCCGCATAGTGCAGCGGCCAGGGTTGGCGTAAAAAGCGCCGACCGCTCTCACGTAAAT
AGAGCCGCTGTACACCATCA

Product: LysM domain-containing protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Beta-glycosidase; Peptidoglycan hydrolase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MRRIIVSALALTTLLPLPSWGASVTVRPGDTLSEIAARYQVSLRALMRLNGLANADNLFIGQTLKLPGSASGTGPAGASR
HTVRSGETLSTIAVRYRVRQQDLITLNGLSNADNLYIGQTLKLPGGASGAIRAGASRHTVRSGETLSIIAARYRVRQQDL
VALNGLANADNLFIGQTLKLPGSASGTVRASATRHTVRSGETLSTIAVRYRVRQQDLITLNGLANANHVERGQTLKLPQG
AVVPKPKAAAKPKPVAIQANPNATSHTVARGQTLNQIAGAYQIPVATLIKINGINNPNKLLVGSKLSLRVKPSTTTQPKS
TTTVAIKPTAKTTVKPTVKSTTKPAAKPQPKPKSTTTVAIKPTAKTTVKPTVKPTVKSTSKPAAKPQPKPKSTQQVAAKP
SQTQWRTYGSLQVDWANWKFMGGSYVAPTLNKDGKALYLAVNCPARKINTTGTNGSWKTWGAPQQRFEHDLVKDLCKAKG
G

Sequences:

>Translated_481_residues
MRRIIVSALALTTLLPLPSWGASVTVRPGDTLSEIAARYQVSLRALMRLNGLANADNLFIGQTLKLPGSASGTGPAGASR
HTVRSGETLSTIAVRYRVRQQDLITLNGLSNADNLYIGQTLKLPGGASGAIRAGASRHTVRSGETLSIIAARYRVRQQDL
VALNGLANADNLFIGQTLKLPGSASGTVRASATRHTVRSGETLSTIAVRYRVRQQDLITLNGLANANHVERGQTLKLPQG
AVVPKPKAAAKPKPVAIQANPNATSHTVARGQTLNQIAGAYQIPVATLIKINGINNPNKLLVGSKLSLRVKPSTTTQPKS
TTTVAIKPTAKTTVKPTVKSTTKPAAKPQPKPKSTTTVAIKPTAKTTVKPTVKPTVKSTSKPAAKPQPKPKSTQQVAAKP
SQTQWRTYGSLQVDWANWKFMGGSYVAPTLNKDGKALYLAVNCPARKINTTGTNGSWKTWGAPQQRFEHDLVKDLCKAKG
G
>Mature_481_residues
MRRIIVSALALTTLLPLPSWGASVTVRPGDTLSEIAARYQVSLRALMRLNGLANADNLFIGQTLKLPGSASGTGPAGASR
HTVRSGETLSTIAVRYRVRQQDLITLNGLSNADNLYIGQTLKLPGGASGAIRAGASRHTVRSGETLSIIAARYRVRQQDL
VALNGLANADNLFIGQTLKLPGSASGTVRASATRHTVRSGETLSTIAVRYRVRQQDLITLNGLANANHVERGQTLKLPQG
AVVPKPKAAAKPKPVAIQANPNATSHTVARGQTLNQIAGAYQIPVATLIKINGINNPNKLLVGSKLSLRVKPSTTTQPKS
TTTVAIKPTAKTTVKPTVKSTTKPAAKPQPKPKSTTTVAIKPTAKTTVKPTVKPTVKSTSKPAAKPQPKPKSTQQVAAKP
SQTQWRTYGSLQVDWANWKFMGGSYVAPTLNKDGKALYLAVNCPARKINTTGTNGSWKTWGAPQQRFEHDLVKDLCKAKG
G

Specific function: Hydrolyzes the cell wall of E.faecalis and M.lysodeikticus. May play an important role in cell wall growth and cell separation [H]

COG id: COG1388

COG function: function code M; FOG: LysM repeat

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 LysM repeats [H]

Homologues:

Organism=Caenorhabditis elegans, GI17559362, Length=389, Percent_Identity=22.6221079691517, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013338
- InterPro:   IPR002901
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF01476 LysM [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 51020; Mature: 51020

Theoretical pI: Translated: 11.74; Mature: 11.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIIVSALALTTLLPLPSWGASVTVRPGDTLSEIAARYQVSLRALMRLNGLANADNLFI
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
GQTLKLPGSASGTGPAGASRHTVRSGETLSTIAVRYRVRQQDLITLNGLSNADNLYIGQT
EEEEECCCCCCCCCCCCCCCCEECCCCHHEEEEEEEEECCCCEEEEECCCCCCCEEEEEE
LKLPGGASGAIRAGASRHTVRSGETLSIIAARYRVRQQDLVALNGLANADNLFIGQTLKL
EECCCCCCCCEECCCCCCEECCCCEEEEEHHHHHHCHHHEEEECCCCCCCCEEEEEEEEC
PGSASGTVRASATRHTVRSGETLSTIAVRYRVRQQDLITLNGLANANHVERGQTLKLPQG
CCCCCCEEEECCHHHHHCCCCHHEEEEEEEEECCCCEEEEECCCCCCCCCCCCEEECCCC
AVVPKPKAAAKPKPVAIQANPNATSHTVARGQTLNQIAGAYQIPVATLIKINGINNPNKL
CCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHCCEEECCEEEEEEEECCCCCCEE
LVGSKLSLRVKPSTTTQPKSTTTVAIKPTAKTTVKPTVKSTTKPAAKPQPKPKSTTTVAI
EEECEEEEEECCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEE
KPTAKTTVKPTVKPTVKSTSKPAAKPQPKPKSTQQVAAKPSQTQWRTYGSLQVDWANWKF
ECCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCEEEEECCEEE
MGGSYVAPTLNKDGKALYLAVNCPARKINTTGTNGSWKTWGAPQQRFEHDLVKDLCKAKG
ECCCEECCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MRRIIVSALALTTLLPLPSWGASVTVRPGDTLSEIAARYQVSLRALMRLNGLANADNLFI
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
GQTLKLPGSASGTGPAGASRHTVRSGETLSTIAVRYRVRQQDLITLNGLSNADNLYIGQT
EEEEECCCCCCCCCCCCCCCCEECCCCHHEEEEEEEEECCCCEEEEECCCCCCCEEEEEE
LKLPGGASGAIRAGASRHTVRSGETLSIIAARYRVRQQDLVALNGLANADNLFIGQTLKL
EECCCCCCCCEECCCCCCEECCCCEEEEEHHHHHHCHHHEEEECCCCCCCCEEEEEEEEC
PGSASGTVRASATRHTVRSGETLSTIAVRYRVRQQDLITLNGLANANHVERGQTLKLPQG
CCCCCCEEEECCHHHHHCCCCHHEEEEEEEEECCCCEEEEECCCCCCCCCCCCEEECCCC
AVVPKPKAAAKPKPVAIQANPNATSHTVARGQTLNQIAGAYQIPVATLIKINGINNPNKL
CCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHCCEEECCEEEEEEEECCCCCCEE
LVGSKLSLRVKPSTTTQPKSTTTVAIKPTAKTTVKPTVKSTTKPAAKPQPKPKSTTTVAI
EEECEEEEEECCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEE
KPTAKTTVKPTVKPTVKSTSKPAAKPQPKPKSTQQVAAKPSQTQWRTYGSLQVDWANWKF
ECCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCEEEEECCEEE
MGGSYVAPTLNKDGKALYLAVNCPARKINTTGTNGSWKTWGAPQQRFEHDLVKDLCKAKG
ECCCEECCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1679432; 12663927 [H]