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The map label for this gene is thiG [H]

Identifier: 33862325

GI number: 33862325

Start: 52427

End: 53359

Strand: Reverse

Name: thiG [H]

Synonym: PMT0052

Alternate gene names: 33862325

Gene position: 53359-52427 (Counterclockwise)

Preceding gene: 33862327

Following gene: 33862324

Centisome position: 2.21

GC content: 55.84

Gene sequence:

>933_bases
ATGGCGAGCAGGATGGGCATGGAAAAACATGATGATGGGCACAAGCTAAGGCCAATCGGCTCAACCGGGACACTCCGCGA
TGACGTTCCAACAACCTCAACAGCGCCAATGGTTCTGACGCACTCCTCACCTGACCTACTCACAATCGGCAAACGCAACT
TCCACAGCCGACTTTTTGCAGGCACAGGGAAGTACCCAAGCCTAAAAGTGATGCAAGAGAGTCTGCGCTCTTCAGGATGC
GAAATGGTGACTGTTGCAGTCCGCCGTGTACAGACAATGGCATCAGGCCACGCCGGCCTGATAGAAGCCATCGACTGGTC
AAAAATATGGATGCTGCCCAACACCGCTGGCTGTGCAACTGCAGAGGAAGCCATTCGTGTAGCCCGACTCGGCCGCGAAC
TCGCCAAACTGGCAGGCCAGGAGGACAACAAGTTCGTCAAGCTCGAAGTCATCCCCGACAGCCGCTACCTCCTACCTGAC
CCTTTCGGCACCCTTGAGGCCGCTGAACAATTGGTAAAAGAAGGCTTCGAGGTCTTGCCATACATCAATGCCGATCCCCT
CTTGGCTAAGCGCCTTGAAGAGGTGGGCTGTGCAACGGTCATGCCACTGGGCTCACCGATTGGATCAGGTCAAGGTCTGA
GGAATGCCGCCAACATCTCCCTAATCATTGAAAACGCCCGAGTGCCAGTCGTTGTGGATGCTGGCATCGGTGTCCCTAGT
GAAGCTGCACAGGCCTTGGAAATGGGTGCCGACGCAGTGCTGGTTAACAGCGCAATTGCCTTAGCCGGAGATCCAATCAC
CATGGCCGAAGCAATGCGCTGGGCAATTCAGGCCGGTAGGCAGGCTTACAGATCAGGTCGCCTACCCGAACGCGCCGCAG
CCTCGCCAAGCTCACCGACAACAGGGATTATTGCGGAAGCAAAGACTAAATAA

Upstream 100 bases:

>100_bases
TCTCTGAACAGAACCCTCCACGACCAGCGGCACAGCCAATTGGGAGGAATCCAAGGGCATAGTGAGCTCGAAGGGAACCT
GACTGTCAATCGAGAGATCC

Downstream 100 bases:

>100_bases
TTATGAAGGAACGTAACGGAAAAGTTCCTGCAAGCAATCCTGTCCATAGGGTCCGCCGAGTTGTAAGTGTTACATGCAAG
TCACCGAGGAAGAGGGTGGT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MASRMGMEKHDDGHKLRPIGSTGTLRDDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYPSLKVMQESLRSSGC
EMVTVAVRRVQTMASGHAGLIEAIDWSKIWMLPNTAGCATAEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRYLLPD
PFGTLEAAEQLVKEGFEVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPS
EAAQALEMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERAAASPSSPTTGIIAEAKTK

Sequences:

>Translated_310_residues
MASRMGMEKHDDGHKLRPIGSTGTLRDDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYPSLKVMQESLRSSGC
EMVTVAVRRVQTMASGHAGLIEAIDWSKIWMLPNTAGCATAEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRYLLPD
PFGTLEAAEQLVKEGFEVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPS
EAAQALEMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERAAASPSSPTTGIIAEAKTK
>Mature_309_residues
ASRMGMEKHDDGHKLRPIGSTGTLRDDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFAGTGKYPSLKVMQESLRSSGCE
MVTVAVRRVQTMASGHAGLIEAIDWSKIWMLPNTAGCATAEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRYLLPDP
FGTLEAAEQLVKEGFEVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPSE
AAQALEMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERAAASPSSPTTGIIAEAKTK

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=259, Percent_Identity=54.0540540540541, Blast_Score=263, Evalue=9e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 32906; Mature: 32775

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRMGMEKHDDGHKLRPIGSTGTLRDDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFA
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHEE
GTGKYPSLKVMQESLRSSGCEMVTVAVRRVQTMASGHAGLIEAIDWSKIWMLPNTAGCAT
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCC
AEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRYLLPDPFGTLEAAEQLVKEGFEVLP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHCCC
YINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPS
CCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCH
EAAQALEMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERAAASPSSPT
HHHHHHHHCCCEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCC
TGIIAEAKTK
CCEEEECCCC
>Mature Secondary Structure 
ASRMGMEKHDDGHKLRPIGSTGTLRDDVPTTSTAPMVLTHSSPDLLTIGKRNFHSRLFA
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHEE
GTGKYPSLKVMQESLRSSGCEMVTVAVRRVQTMASGHAGLIEAIDWSKIWMLPNTAGCAT
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCC
AEEAIRVARLGRELAKLAGQEDNKFVKLEVIPDSRYLLPDPFGTLEAAEQLVKEGFEVLP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHCCC
YINADPLLAKRLEEVGCATVMPLGSPIGSGQGLRNAANISLIIENARVPVVVDAGIGVPS
CCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCH
EAAQALEMGADAVLVNSAIALAGDPITMAEAMRWAIQAGRQAYRSGRLPERAAASPSSPT
HHHHHHHHCCCEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCC
TGIIAEAKTK
CCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]