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The map label for this gene is nhaA

Identifier: 32267144

GI number: 32267144

Start: 1572432

End: 1573766

Strand: Direct

Name: nhaA

Synonym: HH1645

Alternate gene names: 32267144

Gene position: 1572432-1573766 (Clockwise)

Preceding gene: 32267143

Following gene: 32267145

Centisome position: 87.4

GC content: 37.83

Gene sequence:

>1335_bases
ATGGCAGCAAAGCAAAATAGAATTTCGGAAGCATTAAGTAGTTTTATCCGCGCAGAATCTTTTAGTGGTATTTTTTTATT
TTTTTGCGCAGTGAGTGCAATGATTGTGGCTAATTCGCCTTTCTCTGATATTTATAAAAACTTTTGGGAACAACCTTTTG
GTTTCAGCTTTGCAGGGGGATTTTATGGATTTAGTATTCACGATTGGATTAATGATGTATTGATGTCAATTTTTTTTCTT
ATGGTTGGGCTTGAAATTAAACGCGAATTGCTTTTTGGGGATTTATCCGGATTCCAAAAAGCAGCTTTTCCTGTTATAGG
CGCAGTAGGTGGTATGATTGTGCCAGGTGTTATTTATTATGTGCTCAATATGAATACTCCCTCTTATCACGGATTTGGAA
TCCCTATGGCTACCGATATTGCCTTTGCGCTCGGTGTCATCTTGCTTCTTGGCAAACGTGTTCCTTTGGCACTTAAAGTA
TTTCTTGTAACACTTGCTGTGGCTGATGATTTAGGAGCAATTGTTGTTATTGCAGTATTTTATCCTTCGCCTGAAGGTTT
GCATTTTATATATTTAGGTGTAGCAGCAGGGCTTTTAATACTTCTCACAGGTATCAATCATTTAGGGGTGCGCCATTTAG
GAGTGTATATAGGGATTGGTATTTTGTTGTGGTTTTGTGTGCACCATAGCGGGATTCACGCAACTATTGCAGCTGTGGCA
TTAGCGTTTTGTATTCCTGTAAAGCCCAAAATTGAGAGCAAGGAATTTATTCAAGTCGTTCAACAGATGATAGAAATTTT
TGAGAGCAAAGACAAAGAGCGTAAAAATATTTTACTTGATACGCAACAAATGAGTGCAATTGATGAAGCAGGGAGAGATT
TTGCAAAGGTCCAAAATCCACTTTTGCGTTTAGAGCACGCTTTGCAACCTCTGTGTGCATTCATTATTATGCCCCTTTTT
GCTTTTGCTAATGCAGGCGTAGATATTCGTGCAGAAGTAAATTTTCACATTGACCATATTATGCTTGGGGTTATACTTGG
ACTTGTAGTAGGCAAACCTTTGGGTATTTTAAGTTTAACATTTTTATGTGAGAAATGTAAAATTGCTTCACGTCCAGCAG
GAGTGTCTTGGAGTCATATTTTTGGTGCAGGAATGCTCGCAGGCATTGGCTTTACAATGTCTATGTTTGTCTCAAATCTT
GCATTTGATGCTCCTCAAGCAAGTGATGTGTCAAAAATAGCAATTTTGCTTGCTTCAAGCATTGCAGGTATTGTGGGTTC
ATTGTATTTGATAATCAATTATAAAATCAACTCTCGGGCAAAAGTCAAATCTTAA

Upstream 100 bases:

>100_bases
TTAAGCAGGATAATCAGCTTGAGAATATAAATGAAGAAGATGAGATTCAAAAGCTTAAGGAAACACAAAGTATCATTTGA
TTTAAGAGTAAGGAGTAAAA

Downstream 100 bases:

>100_bases
CTATTTTAAGATAGAATCCACAATTTAAGTTTATTAAAAAAGGATTACTTATGCAAGGTGGAGCACAAGAGACATTAATG
TCATTACTTCCTATCGTATT

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA 2

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAAKQNRISEALSSFIRAESFSGIFLFFCAVSAMIVANSPFSDIYKNFWEQPFGFSFAGGFYGFSIHDWINDVLMSIFFL
MVGLEIKRELLFGDLSGFQKAAFPVIGAVGGMIVPGVIYYVLNMNTPSYHGFGIPMATDIAFALGVILLLGKRVPLALKV
FLVTLAVADDLGAIVVIAVFYPSPEGLHFIYLGVAAGLLILLTGINHLGVRHLGVYIGIGILLWFCVHHSGIHATIAAVA
LAFCIPVKPKIESKEFIQVVQQMIEIFESKDKERKNILLDTQQMSAIDEAGRDFAKVQNPLLRLEHALQPLCAFIIMPLF
AFANAGVDIRAEVNFHIDHIMLGVILGLVVGKPLGILSLTFLCEKCKIASRPAGVSWSHIFGAGMLAGIGFTMSMFVSNL
AFDAPQASDVSKIAILLASSIAGIVGSLYLIINYKINSRAKVKS

Sequences:

>Translated_444_residues
MAAKQNRISEALSSFIRAESFSGIFLFFCAVSAMIVANSPFSDIYKNFWEQPFGFSFAGGFYGFSIHDWINDVLMSIFFL
MVGLEIKRELLFGDLSGFQKAAFPVIGAVGGMIVPGVIYYVLNMNTPSYHGFGIPMATDIAFALGVILLLGKRVPLALKV
FLVTLAVADDLGAIVVIAVFYPSPEGLHFIYLGVAAGLLILLTGINHLGVRHLGVYIGIGILLWFCVHHSGIHATIAAVA
LAFCIPVKPKIESKEFIQVVQQMIEIFESKDKERKNILLDTQQMSAIDEAGRDFAKVQNPLLRLEHALQPLCAFIIMPLF
AFANAGVDIRAEVNFHIDHIMLGVILGLVVGKPLGILSLTFLCEKCKIASRPAGVSWSHIFGAGMLAGIGFTMSMFVSNL
AFDAPQASDVSKIAILLASSIAGIVGSLYLIINYKINSRAKVKS
>Mature_443_residues
AAKQNRISEALSSFIRAESFSGIFLFFCAVSAMIVANSPFSDIYKNFWEQPFGFSFAGGFYGFSIHDWINDVLMSIFFLM
VGLEIKRELLFGDLSGFQKAAFPVIGAVGGMIVPGVIYYVLNMNTPSYHGFGIPMATDIAFALGVILLLGKRVPLALKVF
LVTLAVADDLGAIVVIAVFYPSPEGLHFIYLGVAAGLLILLTGINHLGVRHLGVYIGIGILLWFCVHHSGIHATIAAVAL
AFCIPVKPKIESKEFIQVVQQMIEIFESKDKERKNILLDTQQMSAIDEAGRDFAKVQNPLLRLEHALQPLCAFIIMPLFA
FANAGVDIRAEVNFHIDHIMLGVILGLVVGKPLGILSLTFLCEKCKIASRPAGVSWSHIFGAGMLAGIGFTMSMFVSNLA
FDAPQASDVSKIAILLASSIAGIVGSLYLIINYKINSRAKVKS

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=398, Percent_Identity=41.9597989949749, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA2_HELHP (Q7VFN0)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861176.1
- HSSP:   P13738
- ProteinModelPortal:   Q7VFN0
- SMR:   Q7VFN0
- GeneID:   1492192
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1645
- NMPDR:   fig|235279.1.peg.1645
- HOGENOM:   HBG665619
- OMA:   HGFGIPM
- ProtClustDB:   PRK14856
- BioCyc:   HHEP235279:HH_1645-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 48172; Mature: 48041

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe54fc10)-; HASH(0x102ea4e0)-; HASH(0xfdaec9c)-; HASH(0x102c4e40)-; HASH(0x102cba1c)-; HASH(0xf702bc8)-; HASH(0xe67b40c)-; HASH(0x102d217c)-; HASH(0x102ea714)-; HASH(0x10223e74)-; HASH(0x102ece9c)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKQNRISEALSSFIRAESFSGIFLFFCAVSAMIVANSPFSDIYKNFWEQPFGFSFAGG
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEECCC
FYGFSIHDWINDVLMSIFFLMVGLEIKRELLFGDLSGFQKAAFPVIGAVGGMIVPGVIYY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNMNTPSYHGFGIPMATDIAFALGVILLLGKRVPLALKVFLVTLAVADDLGAIVVIAVF
HEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEE
YPSPEGLHFIYLGVAAGLLILLTGINHLGVRHLGVYIGIGILLWFCVHHSGIHATIAAVA
ECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LAFCIPVKPKIESKEFIQVVQQMIEIFESKDKERKNILLDTQQMSAIDEAGRDFAKVQNP
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHEECHHHHHHHHHHHHHHHHHHHH
LLRLEHALQPLCAFIIMPLFAFANAGVDIRAEVNFHIDHIMLGVILGLVVGKPLGILSLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHH
FLCEKCKIASRPAGVSWSHIFGAGMLAGIGFTMSMFVSNLAFDAPQASDVSKIAILLASS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
IAGIVGSLYLIINYKINSRAKVKS
HHHHHHHEEEEEEEEECCCCCCCH
>Mature Secondary Structure 
AAKQNRISEALSSFIRAESFSGIFLFFCAVSAMIVANSPFSDIYKNFWEQPFGFSFAGG
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEECCC
FYGFSIHDWINDVLMSIFFLMVGLEIKRELLFGDLSGFQKAAFPVIGAVGGMIVPGVIYY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNMNTPSYHGFGIPMATDIAFALGVILLLGKRVPLALKVFLVTLAVADDLGAIVVIAVF
HEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEE
YPSPEGLHFIYLGVAAGLLILLTGINHLGVRHLGVYIGIGILLWFCVHHSGIHATIAAVA
ECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LAFCIPVKPKIESKEFIQVVQQMIEIFESKDKERKNILLDTQQMSAIDEAGRDFAKVQNP
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHEECHHHHHHHHHHHHHHHHHHHH
LLRLEHALQPLCAFIIMPLFAFANAGVDIRAEVNFHIDHIMLGVILGLVVGKPLGILSLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHH
FLCEKCKIASRPAGVSWSHIFGAGMLAGIGFTMSMFVSNLAFDAPQASDVSKIAILLASS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
IAGIVGSLYLIINYKINSRAKVKS
HHHHHHHEEEEEEEEECCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12810954