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The map label for this gene is ispDF

Identifier: 32267081

GI number: 32267081

Start: 1511265

End: 1512434

Strand: Direct

Name: ispDF

Synonym: HH1582

Alternate gene names: 32267081

Gene position: 1511265-1512434 (Clockwise)

Preceding gene: 32267078

Following gene: 32267082

Centisome position: 84.0

GC content: 37.78

Gene sequence:

>1170_bases
TTGGTTTTAGATAATGTTTCACTTATTATGATGGCAGCAGGAGATTCTACAAGATTTTGTGCTTCAAATGCACAGACTTT
TAGGTGTAAAAAGCAATGGTTACGCGTGGGGGAAGAGCCATTATGGCTTGTGGCTACACGCAATCTTACTCATCATTTTT
CTTTCAAACAAGTGATTTTGACTGCCTCAAGTAAAGATTATGTTTATATGCAAAATATAAGTCCTTATAAAGTCGTGCAA
GGTGGGCAAACACGCTGTCAATCTCTTAGGAATGCTCTTAAATATGTTGATACGCCCCTTGTTCTTGTAAGCGATGTGGC
ACGATGGGATAGTTCAGATTCTATTATTAAAACAATGTTAAAAGCACTTGATGAAAGTGTAGCCTGTGTTGTGCCTTTTG
TAGGTGTGGCAGATACAAGTTTTTATGAAGGAGAATATCTTAAACGTGAGAGCATTAAACTTATTCAAACACCGCAATTA
AGCAGAGTAGAGGATTTACAAGAAGCTCTTAAGGACACAAGCAAGGATTTTAGTGATGAAAGCTCCGCACTTTACGCACT
TGGTAAAAAAATTGCTTTTGTGCAAGGAAGTGAGTTAATGAATAAATTAACCTTTAGTAGTGATTTGAAGGCACATATCA
CACGTTTATCTCCGCCTTCAAAAAGAGTTTTTATCGGTAATGGCATAGATGTGCATCAATTTGAAAAAAATAAGCAAATG
TGGCTTGGCGGAGTTGAGATAGAATCCCCATTTGGCTTTAAAGCTCATAGTGATGGTGATGTGGTGTTACACGCTTTGAG
CGATGCGATTTTGGGGGCTATTGGTGGAGGTGATATAGGGGAGTGGTTTCCTGATACAGACGAGACATATAAAAATGCAG
ATTCTAAAATGATGTTGAGCGAAATTTATACTTTTGCCCAAAGTGTGGGTTATGAGCTTTATAATGCAGATATAAGTATC
ATAGCGCAAACACCCAAAATTGCTCCTTATAAGAGCGCAATGCGTGAATGTATAGCTCGTATTTTGAGAGTGCCAAATGC
ACACATTAATATTAAGGCAACTACAACAGAGGGATTAGGCTTTGTAGGTCGTGAAGAAGGTGTATGTGTGGAGGCGTGTG
TAAGTATGGGATTTATAAATTGGCATACATATATAAAAGATTTGCATTAA

Upstream 100 bases:

>100_bases
AAAAGTTAAATAATATGGGTATTTAAGTAACAATTTTTAGTTTATTTTTTGTTTTGAAGTGATACAATACTGATTTTAAA
ATAGAGTTTAGGACAAGAAT

Downstream 100 bases:

>100_bases
AAAGGAGGCTGCACGATGTATAAAATTCATTATAAAGGGGAGTTTGTATGAAAGTTTTAATTATTGAAAATGAAATTTAT
CTAGCACAAAGTATTGCCAA

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MVLDNVSLIMMAAGDSTRFCASNAQTFRCKKQWLRVGEEPLWLVATRNLTHHFSFKQVILTASSKDYVYMQNISPYKVVQ
GGQTRCQSLRNALKYVDTPLVLVSDVARWDSSDSIIKTMLKALDESVACVVPFVGVADTSFYEGEYLKRESIKLIQTPQL
SRVEDLQEALKDTSKDFSDESSALYALGKKIAFVQGSELMNKLTFSSDLKAHITRLSPPSKRVFIGNGIDVHQFEKNKQM
WLGGVEIESPFGFKAHSDGDVVLHALSDAILGAIGGGDIGEWFPDTDETYKNADSKMMLSEIYTFAQSVGYELYNADISI
IAQTPKIAPYKSAMRECIARILRVPNAHINIKATTTEGLGFVGREEGVCVEACVSMGFINWHTYIKDLH

Sequences:

>Translated_389_residues
MVLDNVSLIMMAAGDSTRFCASNAQTFRCKKQWLRVGEEPLWLVATRNLTHHFSFKQVILTASSKDYVYMQNISPYKVVQ
GGQTRCQSLRNALKYVDTPLVLVSDVARWDSSDSIIKTMLKALDESVACVVPFVGVADTSFYEGEYLKRESIKLIQTPQL
SRVEDLQEALKDTSKDFSDESSALYALGKKIAFVQGSELMNKLTFSSDLKAHITRLSPPSKRVFIGNGIDVHQFEKNKQM
WLGGVEIESPFGFKAHSDGDVVLHALSDAILGAIGGGDIGEWFPDTDETYKNADSKMMLSEIYTFAQSVGYELYNADISI
IAQTPKIAPYKSAMRECIARILRVPNAHINIKATTTEGLGFVGREEGVCVEACVSMGFINWHTYIKDLH
>Mature_389_residues
MVLDNVSLIMMAAGDSTRFCASNAQTFRCKKQWLRVGEEPLWLVATRNLTHHFSFKQVILTASSKDYVYMQNISPYKVVQ
GGQTRCQSLRNALKYVDTPLVLVSDVARWDSSDSIIKTMLKALDESVACVVPFVGVADTSFYEGEYLKRESIKLIQTPQL
SRVEDLQEALKDTSKDFSDESSALYALGKKIAFVQGSELMNKLTFSSDLKAHITRLSPPSKRVFIGNGIDVHQFEKNKQM
WLGGVEIESPFGFKAHSDGDVVLHALSDAILGAIGGGDIGEWFPDTDETYKNADSKMMLSEIYTFAQSVGYELYNADISI
IAQTPKIAPYKSAMRECIARILRVPNAHINIKATTTEGLGFVGREEGVCVEACVSMGFINWHTYIKDLH

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=148, Percent_Identity=52.027027027027, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_HELHP (Q7VFU3)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861113.1
- ProteinModelPortal:   Q7VFU3
- SMR:   Q7VFU3
- GeneID:   1492129
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1582
- NMPDR:   fig|235279.1.peg.1582
- HOGENOM:   HBG672839
- OMA:   GGDIGEW
- ProtClustDB:   PRK09382
- BioCyc:   HHEP235279:HH_1582-MONOMER
- BRENDA:   2.7.7.60
- BRENDA:   4.6.1.12
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 43300; Mature: 43300

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLDNVSLIMMAAGDSTRFCASNAQTFRCKKQWLRVGEEPLWLVATRNLTHHFSFKQVIL
CEECCEEEEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHCCEEEEEE
TASSKDYVYMQNISPYKVVQGGQTRCQSLRNALKYVDTPLVLVSDVARWDSSDSIIKTML
EECCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
KALDESVACVVPFVGVADTSFYEGEYLKRESIKLIQTPQLSRVEDLQEALKDTSKDFSDE
HHHCCCCEEEEEECCCCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCC
SSALYALGKKIAFVQGSELMNKLTFSSDLKAHITRLSPPSKRVFIGNGIDVHQFEKNKQM
HHHHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCHHHCCCCCCE
WLGGVEIESPFGFKAHSDGDVVLHALSDAILGAIGGGDIGEWFPDTDETYKNADSKMMLS
EECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHH
EIYTFAQSVGYELYNADISIIAQTPKIAPYKSAMRECIARILRVPNAHINIKATTTEGLG
HHHHHHHHHCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FVGREEGVCVEACVSMGFINWHTYIKDLH
CCCCCCCHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MVLDNVSLIMMAAGDSTRFCASNAQTFRCKKQWLRVGEEPLWLVATRNLTHHFSFKQVIL
CEECCEEEEEEECCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHCCEEEEEE
TASSKDYVYMQNISPYKVVQGGQTRCQSLRNALKYVDTPLVLVSDVARWDSSDSIIKTML
EECCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
KALDESVACVVPFVGVADTSFYEGEYLKRESIKLIQTPQLSRVEDLQEALKDTSKDFSDE
HHHCCCCEEEEEECCCCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCC
SSALYALGKKIAFVQGSELMNKLTFSSDLKAHITRLSPPSKRVFIGNGIDVHQFEKNKQM
HHHHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCHHHCCCCCCE
WLGGVEIESPFGFKAHSDGDVVLHALSDAILGAIGGGDIGEWFPDTDETYKNADSKMMLS
EECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHH
EIYTFAQSVGYELYNADISIIAQTPKIAPYKSAMRECIARILRVPNAHINIKATTTEGLG
HHHHHHHHHCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FVGREEGVCVEACVSMGFINWHTYIKDLH
CCCCCCCHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954