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The map label for this gene is nth

Identifier: 32267074

GI number: 32267074

Start: 1506171

End: 1506863

Strand: Direct

Name: nth

Synonym: HH1575

Alternate gene names: 32267074

Gene position: 1506171-1506863 (Clockwise)

Preceding gene: 32267073

Following gene: 32267075

Centisome position: 83.72

GC content: 34.05

Gene sequence:

>693_bases
TTGGCAACACAAAAAGTTATAAAAAAATCTGTATCCAAATCAAAGGATTTTAAAAAGCACAATAAAAAAGAAATTGAGAT
TATCAAATCGCGATTTTTAGAACATTATGGTGATGCAAAAACTGAACTTGTATATCATAATATTTATGAGTTGCTCGTAT
GCGTAATGCTTTCAGCTCAATGCACAGATAAGCGTGTGAATCTTGTAACGCCAGCATTATTTAAGGCTTATCCAAATGTA
GCGACTTTAAGTCAAGCACATCTTGAAGATATTAAGATTTTGATTCAATCTGTTTCATTTTTTAACAATAAGGCTAAACA
TCTTATCACAATGGCGAATCAAGTAATGAATGATTTTAATGGGGAGATACCAACTACACAAGCGGAGTTAAAGACTCTTG
CAGGTGTAGGGCAAAAAACTGCTAATGTAGTGCTTATAGAATTTTTTGAGCAAAATTATATGGCGGTAGATACACACGTG
TTTCGCGTTTCTCATCGCTTGGGATTGAGTGGAGCTAAAAGCGCTCTTGAGACGGAAAAAGAGCTTACACAAGTTTTTAA
AACGCAACTTTCTACTCTTCATCAAGCCTTTGTGCTTTTTGGTCGCTATACTTGCAAGGCTTTGAAACCAATGTGTGAAA
ATTGTTTTGTAGGTGAATTTTGTCAAAAAAAATGTAATTTTAAACCTGCTTGA

Upstream 100 bases:

>100_bases
AATCATTTGGCAGTTGCAGTAATAATTGAGGGCACACAGGTAGCTTTGCGAGATTCTGAAGCAAAATTGATTATTGTTGA
ACCCATAAAGTGAAAGTATC

Downstream 100 bases:

>100_bases
AATATAGACTTCAATATTTTAATTGTATATTGTATAATGCTATATAATATTATCTTGTAAGGTAAAGGTGGTTTGGTATG
GATATTATTCATCGTAGTTT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MATQKVIKKSVSKSKDFKKHNKKEIEIIKSRFLEHYGDAKTELVYHNIYELLVCVMLSAQCTDKRVNLVTPALFKAYPNV
ATLSQAHLEDIKILIQSVSFFNNKAKHLITMANQVMNDFNGEIPTTQAELKTLAGVGQKTANVVLIEFFEQNYMAVDTHV
FRVSHRLGLSGAKSALETEKELTQVFKTQLSTLHQAFVLFGRYTCKALKPMCENCFVGEFCQKKCNFKPA

Sequences:

>Translated_230_residues
MATQKVIKKSVSKSKDFKKHNKKEIEIIKSRFLEHYGDAKTELVYHNIYELLVCVMLSAQCTDKRVNLVTPALFKAYPNV
ATLSQAHLEDIKILIQSVSFFNNKAKHLITMANQVMNDFNGEIPTTQAELKTLAGVGQKTANVVLIEFFEQNYMAVDTHV
FRVSHRLGLSGAKSALETEKELTQVFKTQLSTLHQAFVLFGRYTCKALKPMCENCFVGEFCQKKCNFKPA
>Mature_229_residues
ATQKVIKKSVSKSKDFKKHNKKEIEIIKSRFLEHYGDAKTELVYHNIYELLVCVMLSAQCTDKRVNLVTPALFKAYPNVA
TLSQAHLEDIKILIQSVSFFNNKAKHLITMANQVMNDFNGEIPTTQAELKTLAGVGQKTANVVLIEFFEQNYMAVDTHVF
RVSHRLGLSGAKSALETEKELTQVFKTQLSTLHQAFVLFGRYTCKALKPMCENCFVGEFCQKKCNFKPA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=228, Percent_Identity=27.1929824561404, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787920, Length=203, Percent_Identity=36.9458128078818, Blast_Score=141, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17554540, Length=171, Percent_Identity=32.1637426900585, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45550361, Length=198, Percent_Identity=28.2828282828283, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26075; Mature: 25944

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQKVIKKSVSKSKDFKKHNKKEIEIIKSRFLEHYGDAKTELVYHNIYELLVCVMLSAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CTDKRVNLVTPALFKAYPNVATLSQAHLEDIKILIQSVSFFNNKAKHLITMANQVMNDFN
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEIPTTQAELKTLAGVGQKTANVVLIEFFEQNYMAVDTHVFRVSHRLGLSGAKSALETEK
CCCCCHHHHHHHHHHCCCHHHHHHHHEEHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
ELTQVFKTQLSTLHQAFVLFGRYTCKALKPMCENCFVGEFCQKKCNFKPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATQKVIKKSVSKSKDFKKHNKKEIEIIKSRFLEHYGDAKTELVYHNIYELLVCVMLSAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CTDKRVNLVTPALFKAYPNVATLSQAHLEDIKILIQSVSFFNNKAKHLITMANQVMNDFN
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEIPTTQAELKTLAGVGQKTANVVLIEFFEQNYMAVDTHVFRVSHRLGLSGAKSALETEK
CCCCCHHHHHHHHHHCCCHHHHHHHHEEHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
ELTQVFKTQLSTLHQAFVLFGRYTCKALKPMCENCFVGEFCQKKCNFKPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA