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The map label for this gene is priA

Identifier: 32267050

GI number: 32267050

Start: 1486408

End: 1488321

Strand: Direct

Name: priA

Synonym: HH1551

Alternate gene names: 32267050

Gene position: 1486408-1488321 (Clockwise)

Preceding gene: 32267049

Following gene: 32267051

Centisome position: 82.62

GC content: 37.57

Gene sequence:

>1914_bases
GTGAATTATTACCTTATAGCTCCGATTGGACTAAAATCCCCGCCGCTTGTTTATGAGTGTGAAGAGGAATGTGCCAAAGA
GAGTATCTGTTCTATTGTTGTGCGCGGTAAATCCTATCTTGGTGTAATATTGCAATCGCTTGATAAACCTTCTTTTGAAT
GTAAAATTGCGCATAAGATTACAAATTGTTTTTTGCCCCATCAGATACTTTTAGCTTCATTTATTGCACAATATTATTGT
GTGGGATTAGGGGAAAGCTATGGGCTTTTTGTCCCTCAAGAAAGCAAGAGCGAAGAGAGCGCAAATAAGAATCTCGCAGA
GCTAACATTTGAGCTTAAAACTTTAAGCCGGGAGCAAAATGAAGCTCTCAAATTTTTGTTATCTCACACTAATCCTTTGC
TTTTTGGTGACACAGGAAGTGGAAAGACAGAAATATACATTCATCTTATTGCTCACACACTTGCTCAAGCTAAAAACACA
CTTTTTTTAATGCCAGAAATCGCTCTTACACCACAGATAGAATCCCGCCTTAGAGAAGTATTTGGTGATGCAGTAGGAAT
TTGGCATAGCAAAGTTACTCCAGCACAAAAAAGAAAACTTCTTTTGGCTTTGCATAGTGGCACGATACGCATAATTGCAG
GAGCAAGAAGCGCATTATTTTTGCCTATAAGAGAGCTTGGGCTTGTTATTGTTGATGAAGAACACGATGACGCTTATAAA
TCCCAAAGCACACCATATTATAATGCACGTGATATGGCACTTTATCTCGGGAGCAAAACAAATATCAAAGTTGTTTTAGG
TTCGGCAACACCAAGTGTAGTCAGCTACTATAATGCAATTAAGCAAGACAGCGTATATCGCTTGAGGGGGCGATATTTTA
CTTCGCATAAATCTATTTATATAGTAAGCCAAAATCCGCAAATGACAATAGAAACAGAATCTACACCCATTGATGAAGGG
CTTGTAGCAAAAATCAAAGAAAAACTTGAGCATAATGAACAAGCCATTGTATTTATCCCTACGCGTGCACATTATAAGAT
GCTGGTATGTAGTGAATGTGGAAGTGGTATAGAGTGTGCATTTTGTTCGGTAAATATGTCCTTACATCTTGATAAGAATG
CACTTATTTGCCATTATTGTCATTGGAGTTCACCTATTCCTACATTATGTCCTAAGTGTAAGAGTGAGAGTTTGCACTCT
CATCGTATTGGCACTGCTCAAGTTGCACAAGCCTTACAAAAAGCACTTCCAAACGCAAAAATTACACTTTTTGATAGGGA
TAATATCACCACACACACAAAACTTAAAACGATACTTCAAAAGTTTAATGATGGTGACATTGATGTGCTTGTTGGCACAC
AAATGTTAAGTAAAGGGCACGATTATCATCGTGTGAATCTTGCGATAATATTAGGTATTGATTATGTGCTCAAAGGAAGT
GATTATCGCTGCAATGAGCGAGCAATAAGTTTGTTACATCAAATTGCAGGGCGTAGTGGGCGTAAATACGATGGCGAAGT
GTATGTTCAAAGTGCTAATAGTATATTTTTAGAGCAATTTTTGAGAGATTATGAGGATTTTTTACATTATGAATTGCGCA
ACCGCCCTCATCTCTATCCGCCTTATCAGAGATTAGCTACTCTTACTTTTTCACACAAGATAGAATCCAAAGCTCTTAAT
GCTATGCAAAATGTGCGTGAAATACTTTTAAAACGCGGTTTAGGAGAGGTAGAGATTGTGGGGGATTCTAAGGCATTGGT
AGAGCGACTCTATAATAAGTATCGTTTTATATTATTGCTTCGCTCACCTTCTGCACGAGCACTTTTAGAGTTATTGCATT
ATCTTAATGCTCATACTCATCAAGATTTAGGGCGTATGTATGAAATTGATGTAGACCCTTTAAATATAGTATAG

Upstream 100 bases:

>100_bases
CCCTCATCTGTCAATCACTTGAAAATCTCTATAAAAGCCTTGAAAGTGTAGAGATGCCTACTCCGCATACAAGTGTGGAG
ATTCACGCCATAGAGAATCT

Downstream 100 bases:

>100_bases
AGGTAGATTAGCTATAATAAAGTATTGTATAACCCAACATAATAAAGGAGAGCAAATGAAAATTGCTATATCTTGTTTTT
CGCCATTAATGCAAAATAGT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MNYYLIAPIGLKSPPLVYECEEECAKESICSIVVRGKSYLGVILQSLDKPSFECKIAHKITNCFLPHQILLASFIAQYYC
VGLGESYGLFVPQESKSEESANKNLAELTFELKTLSREQNEALKFLLSHTNPLLFGDTGSGKTEIYIHLIAHTLAQAKNT
LFLMPEIALTPQIESRLREVFGDAVGIWHSKVTPAQKRKLLLALHSGTIRIIAGARSALFLPIRELGLVIVDEEHDDAYK
SQSTPYYNARDMALYLGSKTNIKVVLGSATPSVVSYYNAIKQDSVYRLRGRYFTSHKSIYIVSQNPQMTIETESTPIDEG
LVAKIKEKLEHNEQAIVFIPTRAHYKMLVCSECGSGIECAFCSVNMSLHLDKNALICHYCHWSSPIPTLCPKCKSESLHS
HRIGTAQVAQALQKALPNAKITLFDRDNITTHTKLKTILQKFNDGDIDVLVGTQMLSKGHDYHRVNLAIILGIDYVLKGS
DYRCNERAISLLHQIAGRSGRKYDGEVYVQSANSIFLEQFLRDYEDFLHYELRNRPHLYPPYQRLATLTFSHKIESKALN
AMQNVREILLKRGLGEVEIVGDSKALVERLYNKYRFILLLRSPSARALLELLHYLNAHTHQDLGRMYEIDVDPLNIV

Sequences:

>Translated_637_residues
MNYYLIAPIGLKSPPLVYECEEECAKESICSIVVRGKSYLGVILQSLDKPSFECKIAHKITNCFLPHQILLASFIAQYYC
VGLGESYGLFVPQESKSEESANKNLAELTFELKTLSREQNEALKFLLSHTNPLLFGDTGSGKTEIYIHLIAHTLAQAKNT
LFLMPEIALTPQIESRLREVFGDAVGIWHSKVTPAQKRKLLLALHSGTIRIIAGARSALFLPIRELGLVIVDEEHDDAYK
SQSTPYYNARDMALYLGSKTNIKVVLGSATPSVVSYYNAIKQDSVYRLRGRYFTSHKSIYIVSQNPQMTIETESTPIDEG
LVAKIKEKLEHNEQAIVFIPTRAHYKMLVCSECGSGIECAFCSVNMSLHLDKNALICHYCHWSSPIPTLCPKCKSESLHS
HRIGTAQVAQALQKALPNAKITLFDRDNITTHTKLKTILQKFNDGDIDVLVGTQMLSKGHDYHRVNLAIILGIDYVLKGS
DYRCNERAISLLHQIAGRSGRKYDGEVYVQSANSIFLEQFLRDYEDFLHYELRNRPHLYPPYQRLATLTFSHKIESKALN
AMQNVREILLKRGLGEVEIVGDSKALVERLYNKYRFILLLRSPSARALLELLHYLNAHTHQDLGRMYEIDVDPLNIV
>Mature_637_residues
MNYYLIAPIGLKSPPLVYECEEECAKESICSIVVRGKSYLGVILQSLDKPSFECKIAHKITNCFLPHQILLASFIAQYYC
VGLGESYGLFVPQESKSEESANKNLAELTFELKTLSREQNEALKFLLSHTNPLLFGDTGSGKTEIYIHLIAHTLAQAKNT
LFLMPEIALTPQIESRLREVFGDAVGIWHSKVTPAQKRKLLLALHSGTIRIIAGARSALFLPIRELGLVIVDEEHDDAYK
SQSTPYYNARDMALYLGSKTNIKVVLGSATPSVVSYYNAIKQDSVYRLRGRYFTSHKSIYIVSQNPQMTIETESTPIDEG
LVAKIKEKLEHNEQAIVFIPTRAHYKMLVCSECGSGIECAFCSVNMSLHLDKNALICHYCHWSSPIPTLCPKCKSESLHS
HRIGTAQVAQALQKALPNAKITLFDRDNITTHTKLKTILQKFNDGDIDVLVGTQMLSKGHDYHRVNLAIILGIDYVLKGS
DYRCNERAISLLHQIAGRSGRKYDGEVYVQSANSIFLEQFLRDYEDFLHYELRNRPHLYPPYQRLATLTFSHKIESKALN
AMQNVREILLKRGLGEVEIVGDSKALVERLYNKYRFILLLRSPSARALLELLHYLNAHTHQDLGRMYEIDVDPLNIV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=516, Percent_Identity=32.7519379844961, Blast_Score=271, Evalue=7e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 71980; Mature: 71980

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYYLIAPIGLKSPPLVYECEEECAKESICSIVVRGKSYLGVILQSLDKPSFECKIAHKI
CCEEEEEECCCCCCCEEEECHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCEEEHHHHH
TNCFLPHQILLASFIAQYYCVGLGESYGLFVPQESKSEESANKNLAELTFELKTLSREQN
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHCCCHHHHHHHHHHHCCHHH
EALKFLLSHTNPLLFGDTGSGKTEIYIHLIAHTLAQAKNTLFLMPEIALTPQIESRLREV
HHHHHHHHCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
FGDAVGIWHSKVTPAQKRKLLLALHSGTIRIIAGARSALFLPIRELGLVIVDEEHDDAYK
HHHHHHHHCCCCCCHHHCEEEEEEECCCEEEEECCCCEEEEEHHHCCEEEEECCCCCHHC
SQSTPYYNARDMALYLGSKTNIKVVLGSATPSVVSYYNAIKQDSVYRLRGRYFTSHKSIY
CCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCEEECCCEEE
IVSQNPQMTIETESTPIDEGLVAKIKEKLEHNEQAIVFIPTRAHYKMLVCSECGSGIECA
EEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCEEE
FCSVNMSLHLDKNALICHYCHWSSPIPTLCPKCKSESLHSHRIGTAQVAQALQKALPNAK
EEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCE
ITLFDRDNITTHTKLKTILQKFNDGDIDVLVGTQMLSKGHDYHRVNLAIILGIDYVLKGS
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCEEEEEEEEEEEEEEEECCC
DYRCNERAISLLHQIAGRSGRKYDGEVYVQSANSIFLEQFLRDYEDFLHYELRNRPHLYP
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYQRLATLTFSHKIESKALNAMQNVREILLKRGLGEVEIVGDSKALVERLYNKYRFILLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCEEEEEEE
RSPSARALLELLHYLNAHTHQDLGRMYEIDVDPLNIV
ECCCHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MNYYLIAPIGLKSPPLVYECEEECAKESICSIVVRGKSYLGVILQSLDKPSFECKIAHKI
CCEEEEEECCCCCCCEEEECHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCEEEHHHHH
TNCFLPHQILLASFIAQYYCVGLGESYGLFVPQESKSEESANKNLAELTFELKTLSREQN
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHCCCHHHHHHHHHHHCCHHH
EALKFLLSHTNPLLFGDTGSGKTEIYIHLIAHTLAQAKNTLFLMPEIALTPQIESRLREV
HHHHHHHHCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
FGDAVGIWHSKVTPAQKRKLLLALHSGTIRIIAGARSALFLPIRELGLVIVDEEHDDAYK
HHHHHHHHCCCCCCHHHCEEEEEEECCCEEEEECCCCEEEEEHHHCCEEEEECCCCCHHC
SQSTPYYNARDMALYLGSKTNIKVVLGSATPSVVSYYNAIKQDSVYRLRGRYFTSHKSIY
CCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCEEECCCEEE
IVSQNPQMTIETESTPIDEGLVAKIKEKLEHNEQAIVFIPTRAHYKMLVCSECGSGIECA
EEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCEEE
FCSVNMSLHLDKNALICHYCHWSSPIPTLCPKCKSESLHSHRIGTAQVAQALQKALPNAK
EEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCE
ITLFDRDNITTHTKLKTILQKFNDGDIDVLVGTQMLSKGHDYHRVNLAIILGIDYVLKGS
EEEEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCEEEEEEEEEEEEEEEECCC
DYRCNERAISLLHQIAGRSGRKYDGEVYVQSANSIFLEQFLRDYEDFLHYELRNRPHLYP
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYQRLATLTFSHKIESKALNAMQNVREILLKRGLGEVEIVGDSKALVERLYNKYRFILLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCEEEEEEE
RSPSARALLELLHYLNAHTHQDLGRMYEIDVDPLNIV
ECCCHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]