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The map label for this gene is pth

Identifier: 32266994

GI number: 32266994

Start: 1432666

End: 1433337

Strand: Direct

Name: pth

Synonym: HH1495

Alternate gene names: 32266994

Gene position: 1432666-1433337 (Clockwise)

Preceding gene: 32266993

Following gene: 32266995

Centisome position: 79.63

GC content: 34.67

Gene sequence:

>672_bases
ATGCCTTGCCTCCTTGTCGCAGGACTTGGTAATCCGAGTGCCAAATATCAAAATACTCGGCACAATATTGGGTTTATGGT
GCTTGATTTTTTATCAAAAGAATTAGACTTTGATTTTTCTTTTGATAAAAAATTTAATGCGGAAGTTGGTGCTATAAATA
TCGGTCCTCACAAAGTTTTTTTTCTTAAACCACAAACTTTTATGAATCTCTCCGGGGAGGCAATTAATCCTTTTGTGCGG
TATTTTGATATCACACATACATTCGTAATTCACGATGATATTGATATCGGTTTTGGTGATATGCGTTTTAAATATGGTGG
TTCAAGTGGCGGACATAATGGCTTAAAATCTATTGATTCATTTATGGGAGATACATATTTTAGATTACGTTTTGGTGTGG
GACGAAGTGCGAATAAAAATGTAGTAGAGTATGTTTTAAGTGATTTTAATGCACAAGAAAGAGAGCAACTTGAAGGATTA
ATAGCTCACGCTAAGGAAGCAGTTATGAGTTTTTGTAATATGGCACAATATCCTAAAGAGCATATTCTTACTCATCTACA
GCAGTATTTTACTCTCAAAATACCTAAGCTTACGCCATCGCAAACTCAAAATAATGAATCCTTTGCTACACAATTCTCAC
AAATGCAGTATACAAGATTTACGCAGGGATAA

Upstream 100 bases:

>100_bases
TGGGAGATTCTATCCTTATTCGCGATTTGCCACAATTTGATAATGTAAATGTGTTGAATCGTCCATCTGTGGCAGTAGTT
GGGGTAATTAAAGCTAAATA

Downstream 100 bases:

>100_bases
AAATGATTTTCCGTTTTGTAGGTGTATATTATCTTAAATATTTTTTCATTATTTTTTTAGGCTTAGAAGGCTTTTTTCTT
GCTATTGATATGCTCAAATA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MPCLLVAGLGNPSAKYQNTRHNIGFMVLDFLSKELDFDFSFDKKFNAEVGAINIGPHKVFFLKPQTFMNLSGEAINPFVR
YFDITHTFVIHDDIDIGFGDMRFKYGGSSGGHNGLKSIDSFMGDTYFRLRFGVGRSANKNVVEYVLSDFNAQEREQLEGL
IAHAKEAVMSFCNMAQYPKEHILTHLQQYFTLKIPKLTPSQTQNNESFATQFSQMQYTRFTQG

Sequences:

>Translated_223_residues
MPCLLVAGLGNPSAKYQNTRHNIGFMVLDFLSKELDFDFSFDKKFNAEVGAINIGPHKVFFLKPQTFMNLSGEAINPFVR
YFDITHTFVIHDDIDIGFGDMRFKYGGSSGGHNGLKSIDSFMGDTYFRLRFGVGRSANKNVVEYVLSDFNAQEREQLEGL
IAHAKEAVMSFCNMAQYPKEHILTHLQQYFTLKIPKLTPSQTQNNESFATQFSQMQYTRFTQG
>Mature_222_residues
PCLLVAGLGNPSAKYQNTRHNIGFMVLDFLSKELDFDFSFDKKFNAEVGAINIGPHKVFFLKPQTFMNLSGEAINPFVRY
FDITHTFVIHDDIDIGFGDMRFKYGGSSGGHNGLKSIDSFMGDTYFRLRFGVGRSANKNVVEYVLSDFNAQEREQLEGLI
AHAKEAVMSFCNMAQYPKEHILTHLQQYFTLKIPKLTPSQTQNNESFATQFSQMQYTRFTQG

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=171, Percent_Identity=32.7485380116959, Blast_Score=101, Evalue=6e-22,
Organism=Escherichia coli, GI1787455, Length=169, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_HELHP (Q7VG29)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_861026.1
- ProteinModelPortal:   Q7VG29
- SMR:   Q7VG29
- GeneID:   1492042
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1495
- NMPDR:   fig|235279.1.peg.1495
- HOGENOM:   HBG610927
- OMA:   NESGRQV
- ProtClustDB:   PRK05426
- BioCyc:   HHEP235279:HH_1495-MONOMER
- BRENDA:   3.1.1.29
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 25374; Mature: 25243

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCLLVAGLGNPSAKYQNTRHNIGFMVLDFLSKELDFDFSFDKKFNAEVGAINIGPHKVF
CCEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEE
FLKPQTFMNLSGEAINPFVRYFDITHTFVIHDDIDIGFGDMRFKYGGSSGGHNGLKSIDS
EECCHHHHCCCCCHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHH
FMGDTYFRLRFGVGRSANKNVVEYVLSDFNAQEREQLEGLIAHAKEAVMSFCNMAQYPKE
HHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
HILTHLQQYFTLKIPKLTPSQTQNNESFATQFSQMQYTRFTQG
HHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PCLLVAGLGNPSAKYQNTRHNIGFMVLDFLSKELDFDFSFDKKFNAEVGAINIGPHKVF
CEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEE
FLKPQTFMNLSGEAINPFVRYFDITHTFVIHDDIDIGFGDMRFKYGGSSGGHNGLKSIDS
EECCHHHHCCCCCHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHH
FMGDTYFRLRFGVGRSANKNVVEYVLSDFNAQEREQLEGLIAHAKEAVMSFCNMAQYPKE
HHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
HILTHLQQYFTLKIPKLTPSQTQNNESFATQFSQMQYTRFTQG
HHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954