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The map label for this gene is gltA

Identifier: 32266992

GI number: 32266992

Start: 1430783

End: 1432066

Strand: Direct

Name: gltA

Synonym: HH1493

Alternate gene names: 32266992

Gene position: 1430783-1432066 (Clockwise)

Preceding gene: 32266990

Following gene: 32266993

Centisome position: 79.53

GC content: 37.77

Gene sequence:

>1284_bases
ATGGCTAGCATTACTTTAACAAACAATGAGACAAAAGAGAGTTTTGAGTTTGAGCTTATTGAATGCACAAGAGGACCAAA
GGCTGTAGATTTTTCTAAGCTTTTTGAACGCACCAATATTTTTTCTTATGACCCAGGTTATGGTTCAACCGCAGGATGTA
AATCAACCATTAGCTATATTAATGGCAAAAATGGAGAATTACTTTATAAAGGGCTTCCAATAGAAAAAATTGTAGAAAAA
TACCAATTTACTGATGTGTGCAAGCTACTCATTACAGGTGAAGTTCCTAAAAATGAGACAGAAACCAAGGAATTTGAAAT
TGAGCTTGTGCATCGTAGTTTTTTGAATGAGGGACTCATTAATATTTTTAACGCATTTCCTGATAGCGCACACCCTATGG
CAAATCTCTCTGCTTCGCTTTCTGCACTTTCTACTTTCCATTTCCGCCATTTGGATATGGGTAATGAGGAAGAGCGTCAA
GTAATGGCACGGCGAGCAATTGCTAAAATCCCTACACTTGCAGCATTTTCTTATAGACATTCTTTAGGGATTCCATATAT
TTATCCTGATGTTGCGCGTGGATATGTTGAAAATTTCCTTTATATGTTGCGTGCATATCCTGGTGGTAAAATGAAACATA
CAATTAAGGGTGAAGTTGAAATCACTCCTTTAGAAATAGAGGCTCTTGATAAGATTTTTACGCTTCACGCTGATCACGGA
CAAAATGCTTCTACTACTACTGTGCGCAATGTTGCTTCTACGGGAGCACACCCTTATGCAGCATTAAGTGCTGGTGTTAA
TGCATTGTGGGGTGCAGCACACGGAGGAGCTAATGAAAAAGTGTTAGATATGCTCAATGAGATTGGCGATGCTAAAAATG
TTGATAAGTTTATCAATAAGGCAAAAGATAAAAATGATCCATTTAGGCTTATGGGCTTTGGACATCGTGTGTATAAAAAC
TATGACCCGCGTGCAAAGATTCTGAAAAAACTCAAAGATGACCTTGATGCTAAAGGTATTAAAATGAATACACGATTAAG
TGATTTGGCACATAAGGTTGAAGAAGTAGCATTAAGTGATAGCTACTTTGTAGAGCGAAATCTTTATCCAAATGTTGATT
TTTACTCCGGTATCATTCTTTCTGCACTTAAAATTCCTGTTTCTCTTTTCACGCCAATTTTTGTTATTGGGCGTATGCCG
GGTTGGTGTGCGCAGCTTTTAGAGCACGTTAAAGATCCAACTACAAGAATCACACGACCACGACAAGTGTATATAGGTGA
GTAA

Upstream 100 bases:

>100_bases
CTAATTTTATACAAAATTTAATTTTTTTTTGACTTTTAAAAGAGGTTTGTGCCATAATAAAAGTGTATAGATTCTAAAAA
TTGTTAAAAAAGGAGAATAC

Downstream 100 bases:

>100_bases
CTCAAGTGTATTTAAGGGTTTTTAGCTATAATGCAACCCTTAATTTTCACATAAGGACATACAATGTTAGAAGGACAAAT
TAGAGAGAGTATTTCAAAAT

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MASITLTNNETKESFEFELIECTRGPKAVDFSKLFERTNIFSYDPGYGSTAGCKSTISYINGKNGELLYKGLPIEKIVEK
YQFTDVCKLLITGEVPKNETETKEFEIELVHRSFLNEGLINIFNAFPDSAHPMANLSASLSALSTFHFRHLDMGNEEERQ
VMARRAIAKIPTLAAFSYRHSLGIPYIYPDVARGYVENFLYMLRAYPGGKMKHTIKGEVEITPLEIEALDKIFTLHADHG
QNASTTTVRNVASTGAHPYAALSAGVNALWGAAHGGANEKVLDMLNEIGDAKNVDKFINKAKDKNDPFRLMGFGHRVYKN
YDPRAKILKKLKDDLDAKGIKMNTRLSDLAHKVEEVALSDSYFVERNLYPNVDFYSGIILSALKIPVSLFTPIFVIGRMP
GWCAQLLEHVKDPTTRITRPRQVYIGE

Sequences:

>Translated_427_residues
MASITLTNNETKESFEFELIECTRGPKAVDFSKLFERTNIFSYDPGYGSTAGCKSTISYINGKNGELLYKGLPIEKIVEK
YQFTDVCKLLITGEVPKNETETKEFEIELVHRSFLNEGLINIFNAFPDSAHPMANLSASLSALSTFHFRHLDMGNEEERQ
VMARRAIAKIPTLAAFSYRHSLGIPYIYPDVARGYVENFLYMLRAYPGGKMKHTIKGEVEITPLEIEALDKIFTLHADHG
QNASTTTVRNVASTGAHPYAALSAGVNALWGAAHGGANEKVLDMLNEIGDAKNVDKFINKAKDKNDPFRLMGFGHRVYKN
YDPRAKILKKLKDDLDAKGIKMNTRLSDLAHKVEEVALSDSYFVERNLYPNVDFYSGIILSALKIPVSLFTPIFVIGRMP
GWCAQLLEHVKDPTTRITRPRQVYIGE
>Mature_426_residues
ASITLTNNETKESFEFELIECTRGPKAVDFSKLFERTNIFSYDPGYGSTAGCKSTISYINGKNGELLYKGLPIEKIVEKY
QFTDVCKLLITGEVPKNETETKEFEIELVHRSFLNEGLINIFNAFPDSAHPMANLSASLSALSTFHFRHLDMGNEEERQV
MARRAIAKIPTLAAFSYRHSLGIPYIYPDVARGYVENFLYMLRAYPGGKMKHTIKGEVEITPLEIEALDKIFTLHADHGQ
NASTTTVRNVASTGAHPYAALSAGVNALWGAAHGGANEKVLDMLNEIGDAKNVDKFINKAKDKNDPFRLMGFGHRVYKNY
DPRAKILKKLKDDLDAKGIKMNTRLSDLAHKVEEVALSDSYFVERNLYPNVDFYSGIILSALKIPVSLFTPIFVIGRMPG
WCAQLLEHVKDPTTRITRPRQVYIGE

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=349, Percent_Identity=28.3667621776504, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=42.5882352941177, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1786527, Length=385, Percent_Identity=28.051948051948, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17555174, Length=361, Percent_Identity=28.808864265928, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319850, Length=336, Percent_Identity=27.0833333333333, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=339, Percent_Identity=27.4336283185841, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325257, Length=375, Percent_Identity=24.5333333333333, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640126, Length=401, Percent_Identity=26.4339152119701, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640124, Length=401, Percent_Identity=26.4339152119701, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356863, Length=348, Percent_Identity=25.5747126436782, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47887; Mature: 47756

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASITLTNNETKESFEFELIECTRGPKAVDFSKLFERTNIFSYDPGYGSTAGCKSTISYI
CCEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEECCCCCCCCHHHHHHHHH
NGKNGELLYKGLPIEKIVEKYQFTDVCKLLITGEVPKNETETKEFEIELVHRSFLNEGLI
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NIFNAFPDSAHPMANLSASLSALSTFHFRHLDMGNEEERQVMARRAIAKIPTLAAFSYRH
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
SLGIPYIYPDVARGYVENFLYMLRAYPGGKMKHTIKGEVEITPLEIEALDKIFTLHADHG
HCCCCEECHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEHHHHHHHHHEEECCCC
QNASTTTVRNVASTGAHPYAALSAGVNALWGAAHGGANEKVLDMLNEIGDAKNVDKFINK
CCCCHHHHHHHHHCCCCCHHHHHHCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AKDKNDPFRLMGFGHRVYKNYDPRAKILKKLKDDLDAKGIKMNTRLSDLAHKVEEVALSD
HCCCCCCEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCC
SYFVERNLYPNVDFYSGIILSALKIPVSLFTPIFVIGRMPGWCAQLLEHVKDPTTRITRP
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCC
RQVYIGE
CEEECCC
>Mature Secondary Structure 
ASITLTNNETKESFEFELIECTRGPKAVDFSKLFERTNIFSYDPGYGSTAGCKSTISYI
CEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEECCCCCCCCHHHHHHHHH
NGKNGELLYKGLPIEKIVEKYQFTDVCKLLITGEVPKNETETKEFEIELVHRSFLNEGLI
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NIFNAFPDSAHPMANLSASLSALSTFHFRHLDMGNEEERQVMARRAIAKIPTLAAFSYRH
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
SLGIPYIYPDVARGYVENFLYMLRAYPGGKMKHTIKGEVEITPLEIEALDKIFTLHADHG
HCCCCEECHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEHHHHHHHHHEEECCCC
QNASTTTVRNVASTGAHPYAALSAGVNALWGAAHGGANEKVLDMLNEIGDAKNVDKFINK
CCCCHHHHHHHHHCCCCCHHHHHHCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AKDKNDPFRLMGFGHRVYKNYDPRAKILKKLKDDLDAKGIKMNTRLSDLAHKVEEVALSD
HCCCCCCEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCC
SYFVERNLYPNVDFYSGIILSALKIPVSLFTPIFVIGRMPGWCAQLLEHVKDPTTRITRP
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCC
RQVYIGE
CEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]