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The map label for this gene is hybA [H]

Identifier: 32266977

GI number: 32266977

Start: 1417559

End: 1418596

Strand: Direct

Name: hybA [H]

Synonym: HH1478

Alternate gene names: 32266977

Gene position: 1417559-1418596 (Clockwise)

Preceding gene: 32266976

Following gene: 32266978

Centisome position: 78.79

GC content: 41.33

Gene sequence:

>1038_bases
ATGGAAGAAACCAATCAAAACAATCAGACAAATACATCGCGTAGGAATTTTTTAAAATTTCTAGGCGTGGCAAGTGTGAG
TGCTGTGAGCGTTAATGCGGCACAATCCACAGATTCTAAGCAACCACAATATAAAATGGCTTCCATTATAGATTTGGGAT
TGTGCGATGGGTGCAGAGATAGGGAGATTCCCGCTTGTGTTGAGGCTTGTAAAGCAAAAAATACGACAAATTTTCCCGAG
CCAATAGCGGAGATTCCGTATTATTTTCCGCGAAAAGTCTATGAGGATTATTCCAAAGATAGAGCAAATACTAAGCGTTT
AACCCCTTATAATTGGACTTATGTGGAATCTCTAAAGGTTGAGGATAGAGACATATATGTGCCTAGACGTTGTATGCACT
GCGATGATCCGACTTGCCAGAAGCTCTGCCCCTTTGGCGTGATTTCTAAGGATTCTAATGGCGCGGTAAAAATTGATGAT
GAATATTGCTTTGGCGGTGCAAAATGTAGAGATGTATGCCCTTGGGGGATTCCACAAAGACAAGCAGGAGTTGGAATCTA
TTTGAAAATAGCGCCCAAACTTGCAGGGGGAGGAGCAATGTATAAATGCGATAGCTGCGCGGATTTACTCGCCAAGAATC
AAAAACCAGCTTGTGAGGTGCAATGCCCCAAAAAAGCCATAATCTTTGGAGATAGAGAGGAAATACTTACCAAAGCTAAG
GCATTGCAAAAAGCGTATAAGGGAGACAAAGAGGGGACTTATATCTATGGGGATACGCAAAATGGTGGCACTTCTACATT
TTATGTCTCGCCAGTGTCGTTTGAGCAAATCCAAGAATCTTTAAACAAAAAACACAATTTCACACAAGAACAACCACAAA
AAATGGGGATTCCCCATATGAATCTTGCAATTAAAAACTTTGTGAGTGAGGATTCTGCTTTGATTAAAAGTGTGCTTTTA
GCTCCTGTTGCGGGGGTAATAGCAGGAGGTATTGCTGTGGCAATGGCAAATAGAACCGATAAAAAGGAGGGAAAATGA

Upstream 100 bases:

>100_bases
TGTTTGGGTAGGGCTTAGTGCAAATGTGATAATATCACTTTTATTTTCTTTGCAATTTTATATACTTCCATATTTTTAAG
GCTAAATCAAGGTTAAAATT

Downstream 100 bases:

>100_bases
AAGAGGCAAAAATACAAAGACAAAGTGCGCAAAATAGAATCGTGCATTGGGGTGTTGCAATAAGTATTTTTGGGCTTATT
TTTAGCGGAATCTTGCAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MEETNQNNQTNTSRRNFLKFLGVASVSAVSVNAAQSTDSKQPQYKMASIIDLGLCDGCRDREIPACVEACKAKNTTNFPE
PIAEIPYYFPRKVYEDYSKDRANTKRLTPYNWTYVESLKVEDRDIYVPRRCMHCDDPTCQKLCPFGVISKDSNGAVKIDD
EYCFGGAKCRDVCPWGIPQRQAGVGIYLKIAPKLAGGGAMYKCDSCADLLAKNQKPACEVQCPKKAIIFGDREEILTKAK
ALQKAYKGDKEGTYIYGDTQNGGTSTFYVSPVSFEQIQESLNKKHNFTQEQPQKMGIPHMNLAIKNFVSEDSALIKSVLL
APVAGVIAGGIAVAMANRTDKKEGK

Sequences:

>Translated_345_residues
MEETNQNNQTNTSRRNFLKFLGVASVSAVSVNAAQSTDSKQPQYKMASIIDLGLCDGCRDREIPACVEACKAKNTTNFPE
PIAEIPYYFPRKVYEDYSKDRANTKRLTPYNWTYVESLKVEDRDIYVPRRCMHCDDPTCQKLCPFGVISKDSNGAVKIDD
EYCFGGAKCRDVCPWGIPQRQAGVGIYLKIAPKLAGGGAMYKCDSCADLLAKNQKPACEVQCPKKAIIFGDREEILTKAK
ALQKAYKGDKEGTYIYGDTQNGGTSTFYVSPVSFEQIQESLNKKHNFTQEQPQKMGIPHMNLAIKNFVSEDSALIKSVLL
APVAGVIAGGIAVAMANRTDKKEGK
>Mature_345_residues
MEETNQNNQTNTSRRNFLKFLGVASVSAVSVNAAQSTDSKQPQYKMASIIDLGLCDGCRDREIPACVEACKAKNTTNFPE
PIAEIPYYFPRKVYEDYSKDRANTKRLTPYNWTYVESLKVEDRDIYVPRRCMHCDDPTCQKLCPFGVISKDSNGAVKIDD
EYCFGGAKCRDVCPWGIPQRQAGVGIYLKIAPKLAGGGAMYKCDSCADLLAKNQKPACEVQCPKKAIIFGDREEILTKAK
ALQKAYKGDKEGTYIYGDTQNGGTSTFYVSPVSFEQIQESLNKKHNFTQEQPQKMGIPHMNLAIKNFVSEDSALIKSVLL
APVAGVIAGGIAVAMANRTDKKEGK

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=307, Percent_Identity=28.3387622149837, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787872, Length=197, Percent_Identity=30.4568527918782, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790326, Length=235, Percent_Identity=31.063829787234, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787122, Length=197, Percent_Identity=29.4416243654822, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787749, Length=231, Percent_Identity=29.8701298701299, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI226510944, Length=240, Percent_Identity=25.4166666666667, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38139; Mature: 38139

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETNQNNQTNTSRRNFLKFLGVASVSAVSVNAAQSTDSKQPQYKMASIIDLGLCDGCRD
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCC
REIPACVEACKAKNTTNFPEPIAEIPYYFPRKVYEDYSKDRANTKRLTPYNWTYVESLKV
CCCHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
EDRDIYVPRRCMHCDDPTCQKLCPFGVISKDSNGAVKIDDEYCFGGAKCRDVCPWGIPQR
CCCEEECCHHHCCCCCHHHHHHCCCCEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
QAGVGIYLKIAPKLAGGGAMYKCDSCADLLAKNQKPACEVQCPKKAIIFGDREEILTKAK
CCCCEEEEEECCCCCCCCCEEECHHHHHHHHCCCCCCEEEECCCEEEEECCHHHHHHHHH
ALQKAYKGDKEGTYIYGDTQNGGTSTFYVSPVSFEQIQESLNKKHNFTQEQPQKMGIPHM
HHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCH
NLAIKNFVSEDSALIKSVLLAPVAGVIAGGIAVAMANRTDKKEGK
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
>Mature Secondary Structure
MEETNQNNQTNTSRRNFLKFLGVASVSAVSVNAAQSTDSKQPQYKMASIIDLGLCDGCRD
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCC
REIPACVEACKAKNTTNFPEPIAEIPYYFPRKVYEDYSKDRANTKRLTPYNWTYVESLKV
CCCHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
EDRDIYVPRRCMHCDDPTCQKLCPFGVISKDSNGAVKIDDEYCFGGAKCRDVCPWGIPQR
CCCEEECCHHHCCCCCHHHHHHCCCCEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
QAGVGIYLKIAPKLAGGGAMYKCDSCADLLAKNQKPACEVQCPKKAIIFGDREEILTKAK
CCCCEEEEEECCCCCCCCCEEECHHHHHHHHCCCCCCEEEECCCEEEEECCHHHHHHHHH
ALQKAYKGDKEGTYIYGDTQNGGTSTFYVSPVSFEQIQESLNKKHNFTQEQPQKMGIPHM
HHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCH
NLAIKNFVSEDSALIKSVLLAPVAGVIAGGIAVAMANRTDKKEGK
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]