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The map label for this gene is trpB

Identifier: 32266914

GI number: 32266914

Start: 1354518

End: 1355780

Strand: Direct

Name: trpB

Synonym: HH1415

Alternate gene names: 32266914

Gene position: 1354518-1355780 (Clockwise)

Preceding gene: 32266911

Following gene: 32266917

Centisome position: 75.29

GC content: 39.67

Gene sequence:

>1263_bases
ATGAAAAAGAAAATATTTACAGAATCTAAAAATGGTTATTTTGGGGAAGCAAAAGGTGGTAATCACGCTTTTGGTGGGCA
ATATATCCCTGAAATTTTGCTTCCGGCTTTAAAAGAATTGGAAAAAGCATATCATGGCGTGTTTGTGAGTAAAGCATATA
AAAAGGAGCTTAAAAGCTTATTCAAGCATTTTGTGGGACGTCCAACACCGCTTATTTATGCTCATAATGCTTCAAAGATT
CTCAAAAATGATATTTATTTGAAGTTTGAAGGTTTAGCAAATACAGGGGCGCACAAGATTAATAATGCTCTAGGACAAGT
GCTTTTAGCAAAGCATATGAAGAAAAAACGTGTGATTGCCGAGACAGGAGCGGGACAGCACGGATTAGCCACAGCAGCAG
CTTGTGCACGTTTGGGTTTAGAGTGTGAAATTTTTATGGGTGAGATAGATATAGCACGACAGCGTCCCAATGTCTTTAAT
ATGGAACTTTTTGGCGCAAAAGTCCATAGTGTCAGCAGTGGAAGCAAGACGCTTAAAGATGCAGTTAATGAGGCTTTGCG
TGAGTGGAGTAAAAGAAGTGATGATAGCTTTTATGTGCTAGGAAGCGCACTTGGACCTTATCCATATCCTGATATTGTGC
GAGATTTGCAGAGTGTGATAAGTAAAGAGCTTAAGAAGCAAACAAAGGCTTATTTTAGCTCCTTGCCTGATATTTTGGTA
GCTTGTGTGGGTGGAGGAAGCAATGCTATGGGATTTTTTACTCATTATCTCAAAGAAGAGCGTGTGCGACTTATTGGCGT
TGAAGCTGGAGGTATAGGAGATAAAGAGGGGGAAAATGCTATTAGGATTAATACAATAAATGCAAGTGAAGGCATTGCAC
AGGGCTATAAAAGCTTGTTTTTGCAAGATAAAGATGGGCAGCTTAGTGATACGCATTCTATTTCGGCAGGACTTGATTAT
GCGGGGATTGGACCTCAATTAGCACATTTGTATGAGGTTGGACGTGTGGAATTTCAATCTGCCACAGATAAAGAGGCTTT
AGAAGCACTTAGCTTTTTTGCTCAATATGAGGGCATTATTCCCGCTTTAGAATCTAGCCACGCTCTCGCTGCTGTTATAC
GCCTATGCAAAGATATAAAAGGCAAAAAAATTATTGCTAATATTTCCGGACGCGGCGATAAGGATATATTTATTACTGCT
AAAGCTCTTACACCTGAACATTGGAAGACATTTTTAAGCGAAGAAATCGCTCGTATAGGGTAG

Upstream 100 bases:

>100_bases
ATACTATAATTTTGTTTAAAAAGAATGTTATTTAAAGTGAAGCTAATGCGTTTTTTTCTATAATCTCAGCTACTTTCAAA
TTAAAGAGTGGCGTGTAATA

Downstream 100 bases:

>100_bases
ATTCTATCTTTTCTTATTGAGCATTGTTTTTACGAAAGCTAAGCGAGAGTGAATTTACACAATGGCGTATATTCTTAGGG
GTAAAGCCCTCATTTTCAAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKKKIFTESKNGYFGEAKGGNHAFGGQYIPEILLPALKELEKAYHGVFVSKAYKKELKSLFKHFVGRPTPLIYAHNASKI
LKNDIYLKFEGLANTGAHKINNALGQVLLAKHMKKKRVIAETGAGQHGLATAAACARLGLECEIFMGEIDIARQRPNVFN
MELFGAKVHSVSSGSKTLKDAVNEALREWSKRSDDSFYVLGSALGPYPYPDIVRDLQSVISKELKKQTKAYFSSLPDILV
ACVGGGSNAMGFFTHYLKEERVRLIGVEAGGIGDKEGENAIRINTINASEGIAQGYKSLFLQDKDGQLSDTHSISAGLDY
AGIGPQLAHLYEVGRVEFQSATDKEALEALSFFAQYEGIIPALESSHALAAVIRLCKDIKGKKIIANISGRGDKDIFITA
KALTPEHWKTFLSEEIARIG

Sequences:

>Translated_420_residues
MKKKIFTESKNGYFGEAKGGNHAFGGQYIPEILLPALKELEKAYHGVFVSKAYKKELKSLFKHFVGRPTPLIYAHNASKI
LKNDIYLKFEGLANTGAHKINNALGQVLLAKHMKKKRVIAETGAGQHGLATAAACARLGLECEIFMGEIDIARQRPNVFN
MELFGAKVHSVSSGSKTLKDAVNEALREWSKRSDDSFYVLGSALGPYPYPDIVRDLQSVISKELKKQTKAYFSSLPDILV
ACVGGGSNAMGFFTHYLKEERVRLIGVEAGGIGDKEGENAIRINTINASEGIAQGYKSLFLQDKDGQLSDTHSISAGLDY
AGIGPQLAHLYEVGRVEFQSATDKEALEALSFFAQYEGIIPALESSHALAAVIRLCKDIKGKKIIANISGRGDKDIFITA
KALTPEHWKTFLSEEIARIG
>Mature_420_residues
MKKKIFTESKNGYFGEAKGGNHAFGGQYIPEILLPALKELEKAYHGVFVSKAYKKELKSLFKHFVGRPTPLIYAHNASKI
LKNDIYLKFEGLANTGAHKINNALGQVLLAKHMKKKRVIAETGAGQHGLATAAACARLGLECEIFMGEIDIARQRPNVFN
MELFGAKVHSVSSGSKTLKDAVNEALREWSKRSDDSFYVLGSALGPYPYPDIVRDLQSVISKELKKQTKAYFSSLPDILV
ACVGGGSNAMGFFTHYLKEERVRLIGVEAGGIGDKEGENAIRINTINASEGIAQGYKSLFLQDKDGQLSDTHSISAGLDY
AGIGPQLAHLYEVGRVEFQSATDKEALEALSFFAQYEGIIPALESSHALAAVIRLCKDIKGKKIIANISGRGDKDIFITA
KALTPEHWKTFLSEEIARIG

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=389, Percent_Identity=49.3573264781491, Blast_Score=374, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321412, Length=411, Percent_Identity=48.9051094890511, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_HELHP (Q7VGA7)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860946.1
- ProteinModelPortal:   Q7VGA7
- SMR:   Q7VGA7
- GeneID:   1493103
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1415
- NMPDR:   fig|235279.1.peg.1415
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   HHEP235279:HH_1415-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 45883; Mature: 45883

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIFTESKNGYFGEAKGGNHAFGGQYIPEILLPALKELEKAYHGVFVSKAYKKELKSL
CCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKHFVGRPTPLIYAHNASKILKNDIYLKFEGLANTGAHKINNALGQVLLAKHMKKKRVIA
HHHHCCCCCCEEEECCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ETGAGQHGLATAAACARLGLECEIFMGEIDIARQRPNVFNMELFGAKVHSVSSGSKTLKD
HCCCCCCHHHHHHHHHHHCCEEEEEECCCHHHHCCCCEEEEEECCCHHHCCCCCHHHHHH
AVNEALREWSKRSDDSFYVLGSALGPYPYPDIVRDLQSVISKELKKQTKAYFSSLPDILV
HHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ACVGGGSNAMGFFTHYLKEERVRLIGVEAGGIGDKEGENAIRINTINASEGIAQGYKSLF
HHCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHE
LQDKDGQLSDTHSISAGLDYAGIGPQLAHLYEVGRVEFQSATDKEALEALSFFAQYEGII
EECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCC
PALESSHALAAVIRLCKDIKGKKIIANISGRGDKDIFITAKALTPEHWKTFLSEEIARIG
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKIFTESKNGYFGEAKGGNHAFGGQYIPEILLPALKELEKAYHGVFVSKAYKKELKSL
CCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKHFVGRPTPLIYAHNASKILKNDIYLKFEGLANTGAHKINNALGQVLLAKHMKKKRVIA
HHHHCCCCCCEEEECCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ETGAGQHGLATAAACARLGLECEIFMGEIDIARQRPNVFNMELFGAKVHSVSSGSKTLKD
HCCCCCCHHHHHHHHHHHCCEEEEEECCCHHHHCCCCEEEEEECCCHHHCCCCCHHHHHH
AVNEALREWSKRSDDSFYVLGSALGPYPYPDIVRDLQSVISKELKKQTKAYFSSLPDILV
HHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
ACVGGGSNAMGFFTHYLKEERVRLIGVEAGGIGDKEGENAIRINTINASEGIAQGYKSLF
HHCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHE
LQDKDGQLSDTHSISAGLDYAGIGPQLAHLYEVGRVEFQSATDKEALEALSFFAQYEGII
EECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCC
PALESSHALAAVIRLCKDIKGKKIIANISGRGDKDIFITAKALTPEHWKTFLSEEIARIG
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954