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The map label for this gene is dfp

Identifier: 32266834

GI number: 32266834

Start: 1291455

End: 1292696

Strand: Direct

Name: dfp

Synonym: HH1335

Alternate gene names: 32266834

Gene position: 1291455-1292696 (Clockwise)

Preceding gene: 32266833

Following gene: 32266835

Centisome position: 71.78

GC content: 37.68

Gene sequence:

>1242_bases
ATGCATCACATATTTGTTAATAAAAAAATTCTTATTCTTGTGAGTGGCTCTATTGCCGTTTATAAAATGCTTGATTGTAT
CTCACAGCTGACAAAATATGGTGCAGATGTAAGAGTTGTTATGAGTAGGGAATCTCGCGCATTTATTGCTCCACTAAGTT
TTGAGGCTATGAGCCATAATATTGTTTTGCACGATGACAATCAATCTTGGCATACAACTTTACAAGAAGTAAGCCCAAAT
CATATTTTTTACGCAAAATGGGCAGATATTGTGCTTGTTGCTCCAGCAACAGCAAATACTATCGCAAAAATTGCTTGTGG
CATCGCCGATAATGTTTTGCTCTCTACGCTTTTAGCCACCCGTGCTCCTAAACTTGTAGCTCCTGCAATGAATACAGCTA
TGCTTAATGCTTCTCAAACCAAGCATAATCTTTCTATGCTTGCAGATATGGGCTATGAAATCATTGTTCCACGTTCAAGT
ATCCTTATGTGTGGAGATGAGGGAGAAGGGGCTCTTGCTCAGGTAGAAGAGATTATTTATAGGCTTGGCAGAGAGATTTT
GAGGGATACATTTTGGAGTAAGCAACGTGTTATTGTCAGTGGTGGTGGTTCAAAAGAAAGCATAGATGAGGTGCGCTATA
TTTCTAATCGAAGCAGTGGCAAACAGGCAAGTTATCTTGCTTTAGCTCTATATATGCTTGGCGCGGAGGTAACTTTTATC
TCAAGCTCATTCCCGCTTGTGTTGCCCTTGGACATAAAATGTGTGAGTGTGGAGAGTGTGGTAACATTTGAAAAGGCAAT
TCATAAAACGTTTAATAGTTTAGATTCTATAGAATCTAAACCTATTATTTTTATGGCAGCAGCTCTTGCAGATTATGCGC
CAAAACCTCAAAGTGGCAAACTCAAAAAAGAGCAAATTGGAGAGAATCTCTCTCTTTTGTGTTCCAAAACAAATGATATT
TTGTCATCTATTTCATCGCAAAAAGCCTATAAAGTAGGCTTTAAGGCTGAAATTGATGATGTAAATGCCTTATATTATGC
TCAAAATATGCTAAAGGCAAAAGAGTGTGAAATGGTGTGCCTTAATGTTGTGGGTTCTCAAAACCCTTTTGGTGGAGAAA
CAAATAGAATCAAAATAATTACACATAAGGACATACAAGAGGTAAGTGGTTCAAAGCTTGAAGTTGCTTTTGGTATTGCT
AGTGCATTTTCACAACTACAATCTCTTAAAGGTGATGAATGA

Upstream 100 bases:

>100_bases
GCTTCAAAATAACCTCAAAATAAGCTCCCTTATGTTAGATTCTTAAAGAATCTAGCTTTTGCGTGAAAAAACCAAGCAAT
AAAAAGCTCAAGGAGAGCAT

Downstream 100 bases:

>100_bases
TACAAAAGCTTCAAAAGCTTTCGCATTTGTTAAAAAATAGCCAACAAACACCAGCAACTTATAATGCTTCACTTCCCATA
TTAATACGCGTTATCCAAAA

Product: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MHHIFVNKKILILVSGSIAVYKMLDCISQLTKYGADVRVVMSRESRAFIAPLSFEAMSHNIVLHDDNQSWHTTLQEVSPN
HIFYAKWADIVLVAPATANTIAKIACGIADNVLLSTLLATRAPKLVAPAMNTAMLNASQTKHNLSMLADMGYEIIVPRSS
ILMCGDEGEGALAQVEEIIYRLGREILRDTFWSKQRVIVSGGGSKESIDEVRYISNRSSGKQASYLALALYMLGAEVTFI
SSSFPLVLPLDIKCVSVESVVTFEKAIHKTFNSLDSIESKPIIFMAAALADYAPKPQSGKLKKEQIGENLSLLCSKTNDI
LSSISSQKAYKVGFKAEIDDVNALYYAQNMLKAKECEMVCLNVVGSQNPFGGETNRIKIITHKDIQEVSGSKLEVAFGIA
SAFSQLQSLKGDE

Sequences:

>Translated_413_residues
MHHIFVNKKILILVSGSIAVYKMLDCISQLTKYGADVRVVMSRESRAFIAPLSFEAMSHNIVLHDDNQSWHTTLQEVSPN
HIFYAKWADIVLVAPATANTIAKIACGIADNVLLSTLLATRAPKLVAPAMNTAMLNASQTKHNLSMLADMGYEIIVPRSS
ILMCGDEGEGALAQVEEIIYRLGREILRDTFWSKQRVIVSGGGSKESIDEVRYISNRSSGKQASYLALALYMLGAEVTFI
SSSFPLVLPLDIKCVSVESVVTFEKAIHKTFNSLDSIESKPIIFMAAALADYAPKPQSGKLKKEQIGENLSLLCSKTNDI
LSSISSQKAYKVGFKAEIDDVNALYYAQNMLKAKECEMVCLNVVGSQNPFGGETNRIKIITHKDIQEVSGSKLEVAFGIA
SAFSQLQSLKGDE
>Mature_413_residues
MHHIFVNKKILILVSGSIAVYKMLDCISQLTKYGADVRVVMSRESRAFIAPLSFEAMSHNIVLHDDNQSWHTTLQEVSPN
HIFYAKWADIVLVAPATANTIAKIACGIADNVLLSTLLATRAPKLVAPAMNTAMLNASQTKHNLSMLADMGYEIIVPRSS
ILMCGDEGEGALAQVEEIIYRLGREILRDTFWSKQRVIVSGGGSKESIDEVRYISNRSSGKQASYLALALYMLGAEVTFI
SSSFPLVLPLDIKCVSVESVVTFEKAIHKTFNSLDSIESKPIIFMAAALADYAPKPQSGKLKKEQIGENLSLLCSKTNDI
LSSISSQKAYKVGFKAEIDDVNALYYAQNMLKAKECEMVCLNVVGSQNPFGGETNRIKIITHKDIQEVSGSKLEVAFGIA
SAFSQLQSLKGDE

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine [H]

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family [H]

Homologues:

Organism=Homo sapiens, GI71725351, Length=182, Percent_Identity=35.7142857142857, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI87082301, Length=369, Percent_Identity=35.5013550135501, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17560194, Length=181, Percent_Identity=34.8066298342541, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322762, Length=195, Percent_Identity=32.3076923076923, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322925, Length=132, Percent_Identity=34.8484848484849, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324629, Length=123, Percent_Identity=34.1463414634146, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24657262, Length=179, Percent_Identity=31.8435754189944, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382 [H]

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein [H]

EC number: =4.1.1.36; =6.3.2.5 [H]

Molecular weight: Translated: 45195; Mature: 45195

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHIFVNKKILILVSGSIAVYKMLDCISQLTKYGADVRVVMSRESRAFIAPLSFEAMSHN
CCEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHHCCE
IVLHDDNQSWHTTLQEVSPNHIFYAKWADIVLVAPATANTIAKIACGIADNVLLSTLLAT
EEEECCCCHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
RAPKLVAPAMNTAMLNASQTKHNLSMLADMGYEIIVPRSSILMCGDEGEGALAQVEEIIY
CCCCHHHCCHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHHHH
RLGREILRDTFWSKQRVIVSGGGSKESIDEVRYISNRSSGKQASYLALALYMLGAEVTFI
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEE
SSSFPLVLPLDIKCVSVESVVTFEKAIHKTFNSLDSIESKPIIFMAAALADYAPKPQSGK
ECCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
LKKEQIGENLSLLCSKTNDILSSISSQKAYKVGFKAEIDDVNALYYAQNMLKAKECEMVC
CCHHHHCCCCEEEECCHHHHHHHHHCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHEEE
LNVVGSQNPFGGETNRIKIITHKDIQEVSGSKLEVAFGIASAFSQLQSLKGDE
EEHHCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHHIFVNKKILILVSGSIAVYKMLDCISQLTKYGADVRVVMSRESRAFIAPLSFEAMSHN
CCEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHHCCE
IVLHDDNQSWHTTLQEVSPNHIFYAKWADIVLVAPATANTIAKIACGIADNVLLSTLLAT
EEEECCCCHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
RAPKLVAPAMNTAMLNASQTKHNLSMLADMGYEIIVPRSSILMCGDEGEGALAQVEEIIY
CCCCHHHCCHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHHHH
RLGREILRDTFWSKQRVIVSGGGSKESIDEVRYISNRSSGKQASYLALALYMLGAEVTFI
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEE
SSSFPLVLPLDIKCVSVESVVTFEKAIHKTFNSLDSIESKPIIFMAAALADYAPKPQSGK
ECCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCC
LKKEQIGENLSLLCSKTNDILSSISSQKAYKVGFKAEIDDVNALYYAQNMLKAKECEMVC
CCHHHHCCCCEEEECCHHHHHHHHHCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHEEE
LNVVGSQNPFGGETNRIKIITHKDIQEVSGSKLEVAFGIASAFSQLQSLKGDE
EEHHCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]