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The map label for this gene is mraW

Identifier: 32266778

GI number: 32266778

Start: 1242153

End: 1243106

Strand: Direct

Name: mraW

Synonym: HH1279

Alternate gene names: 32266778

Gene position: 1242153-1243106 (Clockwise)

Preceding gene: 32266777

Following gene: 32266779

Centisome position: 69.04

GC content: 37.21

Gene sequence:

>954_bases
ATGAAACATTATTCAGTTTTAAAAAATGAAATGATACAGGCTTTGGATTGTTTGAAAGAAGATTCTATCTTGATTGATTG
CACTTTGGGCTTTGGAGGGCATACTATTGGGGCATTGCAGGCTTATCCAAATATTGAAGTTTATGCTTTTGATAAAGATA
TATATGCACTTAATCTTGCCAAAGAGCGATTAAAACCTTATTTGCAAAACATTCATTTTTGTCATAATGCTTTTTCTCAA
TTTTTAGACATTGTGCCAAATGTGGTATTGCCTCGTGTAAGGGGCATTATTGCTGATATTGGTGTAAGCTCTATGCAGCT
TGATGAAACGCAACGTGGCTTTAGCTTTGTCTCTTCCACGCTTGATATGCGTATGGATACACGAGCAGACTTGAATGCTA
CAAAAGTGATTAATACTTATTCGCCTATTCGTTTAGAAGAGATTTTCAGAATCTATGGAGAAGTGCGCCAGAGTAAAAAA
CTCGCAGAGATTATTGCATACGAACGCAAGAAAAAGCCTTTTAGCTCTTGCTTAGAATTAAGCACACTTATTGAGCAACA
TTTTCCTCGTGTTGGAGGTATACATCCAGCGACACTAGCTTTTCAGGCACTACGCATTGAGGTTAATGATGAACTAGGCG
AGCTTAAGCGATTGCTCCATAACATAGAACTTGCTTTTGATGAGGGCAAAATTGCATCTTGTAGGGTAGGAATCATCTCT
TTTCATTCTCTAGAAGATAGAATCATTAAGCAATGTTTTAAACAATGGAGCAAATCGTGCATTTGCGCAGAAGAAAGTTT
AAGGTGCGAGTGTGGCAATAATCACGCTAAAGGGCAGATTCTTACTAAGAAGCCAATTATCCCAACCCCACAAGAGATAG
CGCAAAATAAGCGTTCTCGTAGCGCAAAATTAAGAATTTTTGAATTAAAGAGTTCTAAAGATAAAGGAGTATAA

Upstream 100 bases:

>100_bases
TGTGATAGTGATAAGTTTAAAAAATTCAGATTATAAAGAACATCAAATTATTCTTGAGAACCATTTGCGATTTGATTATT
TCAAGCCTTAATAGAGTATA

Downstream 100 bases:

>100_bases
ATGAATAGTGAAAATTATAAAACTATGCGTCTTGATGATATTATTCTTCCTAAATCGCGTGTGAGCGAATGGGAAAAAGA
AGAGATTGCAAATGCCATTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKHYSVLKNEMIQALDCLKEDSILIDCTLGFGGHTIGALQAYPNIEVYAFDKDIYALNLAKERLKPYLQNIHFCHNAFSQ
FLDIVPNVVLPRVRGIIADIGVSSMQLDETQRGFSFVSSTLDMRMDTRADLNATKVINTYSPIRLEEIFRIYGEVRQSKK
LAEIIAYERKKKPFSSCLELSTLIEQHFPRVGGIHPATLAFQALRIEVNDELGELKRLLHNIELAFDEGKIASCRVGIIS
FHSLEDRIIKQCFKQWSKSCICAEESLRCECGNNHAKGQILTKKPIIPTPQEIAQNKRSRSAKLRIFELKSSKDKGV

Sequences:

>Translated_317_residues
MKHYSVLKNEMIQALDCLKEDSILIDCTLGFGGHTIGALQAYPNIEVYAFDKDIYALNLAKERLKPYLQNIHFCHNAFSQ
FLDIVPNVVLPRVRGIIADIGVSSMQLDETQRGFSFVSSTLDMRMDTRADLNATKVINTYSPIRLEEIFRIYGEVRQSKK
LAEIIAYERKKKPFSSCLELSTLIEQHFPRVGGIHPATLAFQALRIEVNDELGELKRLLHNIELAFDEGKIASCRVGIIS
FHSLEDRIIKQCFKQWSKSCICAEESLRCECGNNHAKGQILTKKPIIPTPQEIAQNKRSRSAKLRIFELKSSKDKGV
>Mature_317_residues
MKHYSVLKNEMIQALDCLKEDSILIDCTLGFGGHTIGALQAYPNIEVYAFDKDIYALNLAKERLKPYLQNIHFCHNAFSQ
FLDIVPNVVLPRVRGIIADIGVSSMQLDETQRGFSFVSSTLDMRMDTRADLNATKVINTYSPIRLEEIFRIYGEVRQSKK
LAEIIAYERKKKPFSSCLELSTLIEQHFPRVGGIHPATLAFQALRIEVNDELGELKRLLHNIELAFDEGKIASCRVGIIS
FHSLEDRIIKQCFKQWSKSCICAEESLRCECGNNHAKGQILTKKPIIPTPQEIAQNKRSRSAKLRIFELKSSKDKGV

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=33.0357142857143, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI165377202, Length=197, Percent_Identity=35.0253807106599, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=33.8607594936709, Blast_Score=155, Evalue=3e-39,
Organism=Drosophila melanogaster, GI62472493, Length=340, Percent_Identity=32.6470588235294, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571637, Length=340, Percent_Identity=32.6470588235294, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_HELHP (Q7VGP1)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860810.1
- ProteinModelPortal:   Q7VGP1
- SMR:   Q7VGP1
- GeneID:   1492967
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1279
- NMPDR:   fig|235279.1.peg.1279
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- BioCyc:   HHEP235279:HH_1279-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36003; Mature: 36003

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: BINDING 51-51 BINDING 78-78 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHYSVLKNEMIQALDCLKEDSILIDCTLGFGGHTIGALQAYPNIEVYAFDKDIYALNLA
CCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCEEEHHHH
KERLKPYLQNIHFCHNAFSQFLDIVPNVVLPRVRGIIADIGVSSMQLDETQRGFSFVSST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDMRMDTRADLNATKVINTYSPIRLEEIFRIYGEVRQSKKLAEIIAYERKKKPFSSCLEL
HHHHCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
STLIEQHFPRVGGIHPATLAFQALRIEVNDELGELKRLLHNIELAFDEGKIASCRVGIIS
HHHHHHHCCCCCCCCHHHHHHHHHEEEECHHHHHHHHHHHHHHEEECCCCCCEEEEEHHH
FHSLEDRIIKQCFKQWSKSCICAEESLRCECGNNHAKGQILTKKPIIPTPQEIAQNKRSR
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCC
SAKLRIFELKSSKDKGV
CCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKHYSVLKNEMIQALDCLKEDSILIDCTLGFGGHTIGALQAYPNIEVYAFDKDIYALNLA
CCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCEEEEEECCCEEEHHHH
KERLKPYLQNIHFCHNAFSQFLDIVPNVVLPRVRGIIADIGVSSMQLDETQRGFSFVSST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDMRMDTRADLNATKVINTYSPIRLEEIFRIYGEVRQSKKLAEIIAYERKKKPFSSCLEL
HHHHCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
STLIEQHFPRVGGIHPATLAFQALRIEVNDELGELKRLLHNIELAFDEGKIASCRVGIIS
HHHHHHHCCCCCCCCHHHHHHHHHEEEECHHHHHHHHHHHHHHEEECCCCCCEEEEEHHH
FHSLEDRIIKQCFKQWSKSCICAEESLRCECGNNHAKGQILTKKPIIPTPQEIAQNKRSR
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCC
SAKLRIFELKSSKDKGV
CCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954