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The map label for this gene is aroE

Identifier: 32266746

GI number: 32266746

Start: 1211625

End: 1212431

Strand: Direct

Name: aroE

Synonym: HH1247

Alternate gene names: 32266746

Gene position: 1211625-1212431 (Clockwise)

Preceding gene: 32266745

Following gene: 32266747

Centisome position: 67.34

GC content: 34.82

Gene sequence:

>807_bases
ATGCTTGGTTTTTTTGCTGTTTATGGCAATCCTATCACGCATTCTAAATCCCCTTTTTTGCATAATTACGCCTTTACAAA
ACTTGGCTTAAGTGGCTATTATAGTCGTATTTTGCTTGATAAAGGTGCAAACCTTCGCCAAAATTTTCTTTCAAATGGCT
TAAGTGGAGCAAATATTACTTTACCCTTTAAAGAAGAAGCTTTTAATCAATGTGATGAGGTGCGCGGAGTAGCCCAAAAT
ATTGGCGCTTGTAATACTTGGGTGCTTGAGGACAAAAATCATCTTGTTGGTTATAATACAGATGCGCAAGGATTCTATGA
GTGCATTAAAGAATATAAGATTAAAAATGCCCTTATCATTGGCGCAGGTGGTTCAGCCAAGGCAGTAGCGATGATACTTC
AATCTCATAATATTCCAACAACGCTTATTAATCGCTCCGTGCAAAATCTTAGCTTTTTTGTTCATAAAGGTTTTGAATGC
TATGTAAACAGTGAATTTAAACCCACTTGTAGTTATGATATTCTTATTAATACTACAAGTGCAGGGCTTAATGATAATCT
ATTGCCTTGTGATGAATCACAACTTAAAGAACTCTGCTCTTGTGGTAAATATGCATTTGACCTTATTTATGGGAAATGCA
CACCTTTTCTTGCTTTAGCTCAATCATTCCATCTTAGTTGTAGTGATGGAAAAGAAATGCTTATAAATCAAGCGGCTCTT
TCATTTGAGCTTTTTTGCAAACAAAAATATAACAAAGGCAATTTAGAGATACAACGTATTGCATCGTTTATGAATGAGAT
TTTATAG

Upstream 100 bases:

>100_bases
TGCTTGAAATTCAAGAAGAATGGGCAAAGATTTTGCTTTCAAATGGGCAATATGGTTATGTTTCGTCTCATTATATTGTT
AAAGAAGGTGCAAACTAGCT

Downstream 100 bases:

>100_bases
AAAGCCTACGAATTTATGTTATAATGTATATTTAAGATTTAATCAAGGAGAGAAAATGCGTATTGATGATGCGCTTTTAA
GCAAGCTTGAACGACTATCT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLGFFAVYGNPITHSKSPFLHNYAFTKLGLSGYYSRILLDKGANLRQNFLSNGLSGANITLPFKEEAFNQCDEVRGVAQN
IGACNTWVLEDKNHLVGYNTDAQGFYECIKEYKIKNALIIGAGGSAKAVAMILQSHNIPTTLINRSVQNLSFFVHKGFEC
YVNSEFKPTCSYDILINTTSAGLNDNLLPCDESQLKELCSCGKYAFDLIYGKCTPFLALAQSFHLSCSDGKEMLINQAAL
SFELFCKQKYNKGNLEIQRIASFMNEIL

Sequences:

>Translated_268_residues
MLGFFAVYGNPITHSKSPFLHNYAFTKLGLSGYYSRILLDKGANLRQNFLSNGLSGANITLPFKEEAFNQCDEVRGVAQN
IGACNTWVLEDKNHLVGYNTDAQGFYECIKEYKIKNALIIGAGGSAKAVAMILQSHNIPTTLINRSVQNLSFFVHKGFEC
YVNSEFKPTCSYDILINTTSAGLNDNLLPCDESQLKELCSCGKYAFDLIYGKCTPFLALAQSFHLSCSDGKEMLINQAAL
SFELFCKQKYNKGNLEIQRIASFMNEIL
>Mature_268_residues
MLGFFAVYGNPITHSKSPFLHNYAFTKLGLSGYYSRILLDKGANLRQNFLSNGLSGANITLPFKEEAFNQCDEVRGVAQN
IGACNTWVLEDKNHLVGYNTDAQGFYECIKEYKIKNALIIGAGGSAKAVAMILQSHNIPTTLINRSVQNLSFFVHKGFEC
YVNSEFKPTCSYDILINTTSAGLNDNLLPCDESQLKELCSCGKYAFDLIYGKCTPFLALAQSFHLSCSDGKEMLINQAAL
SFELFCKQKYNKGNLEIQRIASFMNEIL

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=253, Percent_Identity=35.1778656126482, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1787983, Length=265, Percent_Identity=27.9245283018868, Blast_Score=71, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320332, Length=266, Percent_Identity=26.6917293233083, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_HELHP (Q7VGS3)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860778.1
- HSSP:   Q9SQT8
- ProteinModelPortal:   Q7VGS3
- GeneID:   1492935
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1247
- NMPDR:   fig|235279.1.peg.1247
- HOGENOM:   HBG553408
- OMA:   ELAYGKG
- ProtClustDB:   PRK00258
- BioCyc:   HHEP235279:HH_1247-MONOMER
- BRENDA:   1.1.1.25
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29792; Mature: 29792

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: ACT_SITE 64-64

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFFAVYGNPITHSKSPFLHNYAFTKLGLSGYYSRILLDKGANLRQNFLSNGLSGANIT
CCEEEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEE
LPFKEEAFNQCDEVRGVAQNIGACNTWVLEDKNHLVGYNTDAQGFYECIKEYKIKNALII
ECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCEEEE
GAGGSAKAVAMILQSHNIPTTLINRSVQNLSFFVHKGFECYVNSEFKPTCSYDILINTTS
ECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEECC
AGLNDNLLPCDESQLKELCSCGKYAFDLIYGKCTPFLALAQSFHLSCSDGKEMLINQAAL
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
SFELFCKQKYNKGNLEIQRIASFMNEIL
HHHHHHHHCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MLGFFAVYGNPITHSKSPFLHNYAFTKLGLSGYYSRILLDKGANLRQNFLSNGLSGANIT
CCEEEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEE
LPFKEEAFNQCDEVRGVAQNIGACNTWVLEDKNHLVGYNTDAQGFYECIKEYKIKNALII
ECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHCCEEEE
GAGGSAKAVAMILQSHNIPTTLINRSVQNLSFFVHKGFECYVNSEFKPTCSYDILINTTS
ECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEEECC
AGLNDNLLPCDESQLKELCSCGKYAFDLIYGKCTPFLALAQSFHLSCSDGKEMLINQAAL
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
SFELFCKQKYNKGNLEIQRIASFMNEIL
HHHHHHHHCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954