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The map label for this gene is hhaIIM [H]

Identifier: 32266743

GI number: 32266743

Start: 1209054

End: 1209737

Strand: Direct

Name: hhaIIM [H]

Synonym: HH1244

Alternate gene names: 32266743

Gene position: 1209054-1209737 (Clockwise)

Preceding gene: 32266742

Following gene: 32266744

Centisome position: 67.2

GC content: 34.36

Gene sequence:

>684_bases
ATGAGTAAAGATTTGCAAGATATTATTAAAAGCAAATTTCGCGAAAAAAAGCTTTTTAATACGCGTTTAAAAATTGATGG
CTTAGAGCTTTTGTCTATGATAGAATCCCAAAGTATTGATTTATGTTTTTTTGACCCGCAATATCGCGGAGTATTAGATA
AAATGAAATATGGCAATGAAGGTGAGCGGCAAAGAGGTAGAGCAAATCTAATGCAAATGAGCGAGGAAAATATTATACAT
TTTTTACAAGAGATTGCTCGTGTGCTTAAATTAAGTAAATATTTAATGCTGTGGATTGATAAGTTTCATTTATGTGAGGG
GATTCATCAATGGCTTAAAAATACAAATTTGCAAATTGTAGATTTGATTACTTGGGATAAACAAAAAATGGGTATGGGCT
ATCGCACTAGGCGACAAAGTGAATATTTGCTCATTTTGCAAAAAAAACCGCTCAAAGCGAAAGGCACTTGGGAGAGAAAA
AATATCCGTGATATATGGAGTGAAAAAATCCCATCAGATTCTATAAAAATTCATCCTCATACAAAGCCAAAGGGTTTGCA
AAGTGCGCTTATAGAATCTTGCACCAAAGTAGGCGAAGTGGTGCTTGATCCAGCAAGCGGTAGTTTTAGCGTATTAGAGT
GTTGCAGAGAATTAAACCGGCATTTTATAGGGAGTAATTTATAA

Upstream 100 bases:

>100_bases
TGTTTTGTATTGCTTTTAGACGAGGAAAAGATTCCACAAATTTTTAATTAACACTACAATTATGTTCAGAAAAAATTAAA
GCAATTCTTAAGGATAAATG

Downstream 100 bases:

>100_bases
GTCTTTTCTAAAATCATTTTTTTATGTTAGAATCCGCTATTTATGGATATGAGCTAAAAAGGGACTTTAAATGATAAGCT
ATAAAGATTCTGGAGTGAAT

Product: adenine-specific DNA methylase

Products: NA

Alternate protein names: M.HhaII; Adenine-specific methyltransferase HhaII [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSKDLQDIIKSKFREKKLFNTRLKIDGLELLSMIESQSIDLCFFDPQYRGVLDKMKYGNEGERQRGRANLMQMSEENIIH
FLQEIARVLKLSKYLMLWIDKFHLCEGIHQWLKNTNLQIVDLITWDKQKMGMGYRTRRQSEYLLILQKKPLKAKGTWERK
NIRDIWSEKIPSDSIKIHPHTKPKGLQSALIESCTKVGEVVLDPASGSFSVLECCRELNRHFIGSNL

Sequences:

>Translated_227_residues
MSKDLQDIIKSKFREKKLFNTRLKIDGLELLSMIESQSIDLCFFDPQYRGVLDKMKYGNEGERQRGRANLMQMSEENIIH
FLQEIARVLKLSKYLMLWIDKFHLCEGIHQWLKNTNLQIVDLITWDKQKMGMGYRTRRQSEYLLILQKKPLKAKGTWERK
NIRDIWSEKIPSDSIKIHPHTKPKGLQSALIESCTKVGEVVLDPASGSFSVLECCRELNRHFIGSNL
>Mature_226_residues
SKDLQDIIKSKFREKKLFNTRLKIDGLELLSMIESQSIDLCFFDPQYRGVLDKMKYGNEGERQRGRANLMQMSEENIIHF
LQEIARVLKLSKYLMLWIDKFHLCEGIHQWLKNTNLQIVDLITWDKQKMGMGYRTRRQSEYLLILQKKPLKAKGTWERKN
IRDIWSEKIPSDSIKIHPHTKPKGLQSALIESCTKVGEVVLDPASGSFSVLECCRELNRHFIGSNL

Specific function: This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HhaII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 26599; Mature: 26467

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDLQDIIKSKFREKKLFNTRLKIDGLELLSMIESQSIDLCFFDPQYRGVLDKMKYGNE
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCC
GERQRGRANLMQMSEENIIHFLQEIARVLKLSKYLMLWIDKFHLCEGIHQWLKNTNLQIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
DLITWDKQKMGMGYRTRRQSEYLLILQKKPLKAKGTWERKNIRDIWSEKIPSDSIKIHPH
EEEECCHHHHCCCHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCC
TKPKGLQSALIESCTKVGEVVLDPASGSFSVLECCRELNRHFIGSNL
CCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKDLQDIIKSKFREKKLFNTRLKIDGLELLSMIESQSIDLCFFDPQYRGVLDKMKYGNE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCC
GERQRGRANLMQMSEENIIHFLQEIARVLKLSKYLMLWIDKFHLCEGIHQWLKNTNLQIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
DLITWDKQKMGMGYRTRRQSEYLLILQKKPLKAKGTWERKNIRDIWSEKIPSDSIKIHPH
EEEECCHHHHCCCHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCC
TKPKGLQSALIESCTKVGEVVLDPASGSFSVLECCRELNRHFIGSNL
CCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3248721; 6315538 [H]