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The map label for this gene is argG

Identifier: 32266720

GI number: 32266720

Start: 1189082

End: 1190296

Strand: Direct

Name: argG

Synonym: HH1221

Alternate gene names: 32266720

Gene position: 1189082-1190296 (Clockwise)

Preceding gene: 32266718

Following gene: 32266721

Centisome position: 66.09

GC content: 40.0

Gene sequence:

>1215_bases
ATGGCAGCGATTAAAAAGGTTGTTTTGGCATATAGTGGCGGACTTGATACGAGTGTGATTTTAAAATGGCTCGGAGATAA
TTATCATTGTGAGGTGGTAACCTTTACAGCTGATATTGGACAGGGTGAAGAGGTTGAGCCTGCACGAGAAAAGGCTTTAA
AGCTTGGTATAAAAAGTGAAAATATTTTTATTGAAGATTTAAGAGAGGAGTTTATCAAAGATTTTGTCTTTCCTATGTTT
CGTGCAAATACAATTTATGAGGGAGAATATTTGCTTGGCACAAGCATTGCACGCCCTCTTATTGCAAAAAGACTTGTTGA
GATTGCTAAAAGTGTTGGGGCTGATGCCATAGCACACGGAGCGACAGGAAAGGGAAATGACCAAGTGCGATTTGAGCTCG
GAGCCTACGCGCTTAATCCAGATATTAAAGTCATTGCGCCTTGGCGTGAATGGGATTTGAATAGTCGTGAAAAACTCTTA
GCCTATGCAGAATCTGCAGGGATTGCTATTGAAAAAAAGCAAAATAAATCGCCTTATTCAATGGACGCAAACTTATTGCA
TATTAGCTATGAGGGACAGATTCTAGAAGACCCAAATGTAGAACCAGAAGAGGATATGTGGCGATGGAGTGTTTCACCAC
TTAATGCGCCAGATAAGCCAGAATCTATTAGCATTGAGTTTCAAAATGGCGATGGCGTGGCGATAAATGGAGAAAAGCTA
AGTCCTGCGAATTTTTGGGTAAATCTCAATGAGCTAGGCGGAAAGCACGGCATTGGGCGGCTTGATTTGGTAGAAAATCG
CTATGTGGGTATGAAATCTCGTGGGTGTTATGAAACACCCGGAGGCACAATATATTTAAAAGCTCATCGTGCGATAGAAT
CTTTGTGTCTTGATAGGGAAGAGGCACATTTAAAAGATTCTATAATGCCAAAGTATGCAGAGCTCATTTATAATGGCTAT
TGGTTTAGCCCAGAGCGCGAGGCATTACAAGCACTTATTGATAAGACACAGCAAAAAGTAAGTGGAGTAGTGCGCCTTAA
GCTCTATAAAGGGAATGTCATTGTTATAGGGCGAGAATCTAAAAATTCTTTATTTAATGCAGCATATAGCACTTTTGAGG
AAGATTCTGTATATAATCAAAAAGATGCCGCAGGGTTTATTAAACTTAATGCTTTACGCTTTATCATCGCAGGAAAGGCA
AATAGGGACAAATAA

Upstream 100 bases:

>100_bases
TCATAGTGATTGAAAAATACAATCATAACAAAATATTGCATATATAGTATCTTTAGTATAGAATATGCACTTTAGATTCT
ATAAAAATATGGAGTAGATT

Downstream 100 bases:

>100_bases
GTGATGATGGGGTTAAGATTTTGTATCTTAGTATCTTTATTTTGCACATATATTTATGCCGAGCCTAATAAAAATAGTCC
ATTTGTGCTCAACTTTGATG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MAAIKKVVLAYSGGLDTSVILKWLGDNYHCEVVTFTADIGQGEEVEPAREKALKLGIKSENIFIEDLREEFIKDFVFPMF
RANTIYEGEYLLGTSIARPLIAKRLVEIAKSVGADAIAHGATGKGNDQVRFELGAYALNPDIKVIAPWREWDLNSREKLL
AYAESAGIAIEKKQNKSPYSMDANLLHISYEGQILEDPNVEPEEDMWRWSVSPLNAPDKPESISIEFQNGDGVAINGEKL
SPANFWVNLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIYLKAHRAIESLCLDREEAHLKDSIMPKYAELIYNGY
WFSPEREALQALIDKTQQKVSGVVRLKLYKGNVIVIGRESKNSLFNAAYSTFEEDSVYNQKDAAGFIKLNALRFIIAGKA
NRDK

Sequences:

>Translated_404_residues
MAAIKKVVLAYSGGLDTSVILKWLGDNYHCEVVTFTADIGQGEEVEPAREKALKLGIKSENIFIEDLREEFIKDFVFPMF
RANTIYEGEYLLGTSIARPLIAKRLVEIAKSVGADAIAHGATGKGNDQVRFELGAYALNPDIKVIAPWREWDLNSREKLL
AYAESAGIAIEKKQNKSPYSMDANLLHISYEGQILEDPNVEPEEDMWRWSVSPLNAPDKPESISIEFQNGDGVAINGEKL
SPANFWVNLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIYLKAHRAIESLCLDREEAHLKDSIMPKYAELIYNGY
WFSPEREALQALIDKTQQKVSGVVRLKLYKGNVIVIGRESKNSLFNAAYSTFEEDSVYNQKDAAGFIKLNALRFIIAGKA
NRDK
>Mature_403_residues
AAIKKVVLAYSGGLDTSVILKWLGDNYHCEVVTFTADIGQGEEVEPAREKALKLGIKSENIFIEDLREEFIKDFVFPMFR
ANTIYEGEYLLGTSIARPLIAKRLVEIAKSVGADAIAHGATGKGNDQVRFELGAYALNPDIKVIAPWREWDLNSREKLLA
YAESAGIAIEKKQNKSPYSMDANLLHISYEGQILEDPNVEPEEDMWRWSVSPLNAPDKPESISIEFQNGDGVAINGEKLS
PANFWVNLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIYLKAHRAIESLCLDREEAHLKDSIMPKYAELIYNGYW
FSPEREALQALIDKTQQKVSGVVRLKLYKGNVIVIGRESKNSLFNAAYSTFEEDSVYNQKDAAGFIKLNALRFIIAGKAN
RDK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=403, Percent_Identity=46.8982630272953, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI16950633, Length=403, Percent_Identity=46.8982630272953, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI1789563, Length=415, Percent_Identity=28.433734939759, Blast_Score=131, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=414, Percent_Identity=44.9275362318841, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI21358151, Length=403, Percent_Identity=45.1612903225806, Blast_Score=345, Evalue=3e-95,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_HELHP (Q7VGU9)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860752.1
- ProteinModelPortal:   Q7VGU9
- SMR:   Q7VGU9
- GeneID:   1492909
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1221
- NMPDR:   fig|235279.1.peg.1221
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BioCyc:   HHEP235279:HH_1221-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45161; Mature: 45030

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 90-90 BINDING 95-95 BINDING 120-120 BINDING 122-122 BINDING 126-126 BINDING 126-126 BINDING 127-127 BINDING 130-130 BINDING 180-180 BINDING 189-189 BINDING 265-265 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIKKVVLAYSGGLDTSVILKWLGDNYHCEVVTFTADIGQGEEVEPAREKALKLGIKSE
CCHHHEEHHHCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHHHHEECCCCC
NIFIEDLREEFIKDFVFPMFRANTIYEGEYLLGTSIARPLIAKRLVEIAKSVGADAIAHG
CCHHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCC
ATGKGNDQVRFELGAYALNPDIKVIAPWREWDLNSREKLLAYAESAGIAIEKKQNKSPYS
CCCCCCCEEEEEEEEEEECCCEEEEECCHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCC
MDANLLHISYEGQILEDPNVEPEEDMWRWSVSPLNAPDKPESISIEFQNGDGVAINGEKL
CCCEEEEEEECCEEECCCCCCCCHHHEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCC
SPANFWVNLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIYLKAHRAIESLCLDRE
CCCEEEEEHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCCCH
EAHLKDSIMPKYAELIYNGYWFSPEREALQALIDKTQQKVSGVVRLKLYKGNVIVIGRES
HHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEEECC
KNSLFNAAYSTFEEDSVYNQKDAAGFIKLNALRFIIAGKANRDK
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
AAIKKVVLAYSGGLDTSVILKWLGDNYHCEVVTFTADIGQGEEVEPAREKALKLGIKSE
CHHHEEHHHCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHHHHEECCCCC
NIFIEDLREEFIKDFVFPMFRANTIYEGEYLLGTSIARPLIAKRLVEIAKSVGADAIAHG
CCHHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCC
ATGKGNDQVRFELGAYALNPDIKVIAPWREWDLNSREKLLAYAESAGIAIEKKQNKSPYS
CCCCCCCEEEEEEEEEEECCCEEEEECCHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCC
MDANLLHISYEGQILEDPNVEPEEDMWRWSVSPLNAPDKPESISIEFQNGDGVAINGEKL
CCCEEEEEEECCEEECCCCCCCCHHHEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCC
SPANFWVNLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIYLKAHRAIESLCLDRE
CCCEEEEEHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCCCH
EAHLKDSIMPKYAELIYNGYWFSPEREALQALIDKTQQKVSGVVRLKLYKGNVIVIGRES
HHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCEEEEEECC
KNSLFNAAYSTFEEDSVYNQKDAAGFIKLNALRFIIAGKANRDK
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954