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The map label for this gene is ruvC

Identifier: 32266610

GI number: 32266610

Start: 1082089

End: 1082646

Strand: Direct

Name: ruvC

Synonym: HH1111

Alternate gene names: 32266610

Gene position: 1082089-1082646 (Clockwise)

Preceding gene: 32266607

Following gene: 32266611

Centisome position: 60.14

GC content: 35.13

Gene sequence:

>558_bases
TTGCATATCTTAGGTATAGACCCCGGAAGTAGAAATTGCGGTTATGCAATTATCAATTATGCACCTCACGTTAATTCACT
TAAACTCATTGAAGCAGGTATTATTAAAATCAAAGAGCGTGATTTACAAACCCAAATTATGGAATTTGTAGAAGGCATTG
ATTTGGTAATTAAAAATATTCATATTGATGAGGTGGCTATTGAAGATATATTTTTTGCTTACAACCCTCAAAGTGTCATC
AAACTTGCTCAATTTCGTGGGGCATTGAGCTTAAAAATTCTCCAAGAGATTGGAAATTTTTCTGAATACACACCCTTACA
GGTCAAAAAAGCAATCACAGGAAATGGTAAGGCAGACAAAACCCAAGTTGCATTTATGGTAAAGCGCATTTTAGGGCTTA
AGGGTGAAATAAAACCTCTTGATATTACAGATGCTATTGCCATTGCCATTACACATTCTCAACGTATCAAAACAAACGAT
GTTCATTCTACACCTAATCTTCAACGTGCAATGCCTTTAAAATCTCTTTCTGCAAACCCTTCTAAAGGCTGTATATGA

Upstream 100 bases:

>100_bases
CTATGTGAATAAAATGTGAAAAAACAAAAATCCTTTTGATTTTTTAATTAAAATTTGGAAGTGAAATTATAATGAAAAAA
ACTTTTAATTTGGATTAAAT

Downstream 100 bases:

>100_bases
AACAAAAAACATATACATTTTTTGATTCTCAAACACATATCTCTTATGCTTTTGAAGACAATGCTCTCACACCTTGTAAA
AAGCCTCCAAAAAAATATGA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MHILGIDPGSRNCGYAIINYAPHVNSLKLIEAGIIKIKERDLQTQIMEFVEGIDLVIKNIHIDEVAIEDIFFAYNPQSVI
KLAQFRGALSLKILQEIGNFSEYTPLQVKKAITGNGKADKTQVAFMVKRILGLKGEIKPLDITDAIAIAITHSQRIKTND
VHSTPNLQRAMPLKSLSANPSKGCI

Sequences:

>Translated_185_residues
MHILGIDPGSRNCGYAIINYAPHVNSLKLIEAGIIKIKERDLQTQIMEFVEGIDLVIKNIHIDEVAIEDIFFAYNPQSVI
KLAQFRGALSLKILQEIGNFSEYTPLQVKKAITGNGKADKTQVAFMVKRILGLKGEIKPLDITDAIAIAITHSQRIKTND
VHSTPNLQRAMPLKSLSANPSKGCI
>Mature_185_residues
MHILGIDPGSRNCGYAIINYAPHVNSLKLIEAGIIKIKERDLQTQIMEFVEGIDLVIKNIHIDEVAIEDIFFAYNPQSVI
KLAQFRGALSLKILQEIGNFSEYTPLQVKKAITGNGKADKTQVAFMVKRILGLKGEIKPLDITDAIAIAITHSQRIKTND
VHSTPNLQRAMPLKSLSANPSKGCI

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=39.3939393939394, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_HELHP (Q7VH56)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860642.1
- ProteinModelPortal:   Q7VH56
- SMR:   Q7VH56
- GeneID:   1492799
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1111
- NMPDR:   fig|235279.1.peg.1111
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   HHEP235279:HH_1111-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 20416; Mature: 20416

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILGIDPGSRNCGYAIINYAPHVNSLKLIEAGIIKIKERDLQTQIMEFVEGIDLVIKNI
CEEEECCCCCCCCCEEEEECCCCCCCEEHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHC
HIDEVAIEDIFFAYNPQSVIKLAQFRGALSLKILQEIGNFSEYTPLQVKKAITGNGKADK
CHHHHHHHHHHEEECHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCH
TQVAFMVKRILGLKGEIKPLDITDAIAIAITHSQRIKTNDVHSTPNLQRAMPLKSLSANP
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCHHHCCCCC
SKGCI
CCCCC
>Mature Secondary Structure
MHILGIDPGSRNCGYAIINYAPHVNSLKLIEAGIIKIKERDLQTQIMEFVEGIDLVIKNI
CEEEECCCCCCCCCEEEEECCCCCCCEEHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHC
HIDEVAIEDIFFAYNPQSVIKLAQFRGALSLKILQEIGNFSEYTPLQVKKAITGNGKADK
CHHHHHHHHHHEEECHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCH
TQVAFMVKRILGLKGEIKPLDITDAIAIAITHSQRIKTNDVHSTPNLQRAMPLKSLSANP
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHCCHHHCCCCC
SKGCI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954