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The map label for this gene is glnH [H]

Identifier: 32266237

GI number: 32266237

Start: 722420

End: 723247

Strand: Direct

Name: glnH [H]

Synonym: HH0738

Alternate gene names: 32266237

Gene position: 722420-723247 (Clockwise)

Preceding gene: 32266236

Following gene: 32266239

Centisome position: 40.15

GC content: 35.99

Gene sequence:

>828_bases
ATGAAAAAATTGCTTTTAGCGCTATTTGGCGTAGTATTTATGATAGGACTGAGTGCGTGTGGAGATAATCAAAGCACAAA
AGAGTCAAAAAATGCCTTAGAAGAAATTAAACAAAAAGGTGTTGTGCGCATAGGAGTATTTAGCGATAAACCACCCTTTG
GTTTCATCAATGAAAAAGGACAAAATGATGGTTTTGATGTTTATATTGCAAAACGCATCGCTAAAGATTTACTTGGAGAT
GAAAATAAAGTTGAATTTGTGCTTACTGAAGCTGCTTCTCGCGTAGAGTTTTTGCGCTCTAATAAGGTAGATATTATTAT
GGCAAACTTCACTCAAACTCCAGAACGCGAGGCAGTAGTAGATTTTGCTAAACCCTATATGAAAGTGGCTCTTGGTGTTA
TATCTCCAAATGGCGAGATTAAAAGCATTGATGACTTAAAAGGGAAAAAACTAATTGTCAATAAAGGCACAACAGCTGAC
GCATTCTTTAGCAAAAACTACCCTGATATTGAATTGCTTAAATATGAGCAAAATACTGAAGCTTTTCTCGCGCTTAAAGA
TAAAAGAGGTGATGCACTTGCTCACGATAATACTCTTTTGCTCGCGTGGGCTATGGAAAATGAAGGTTTTAACGTGGGCA
TTCCAACACTAGGTGAAGAAGAAAATATTGCTCCAGCAATAAAAAAAGGAAATATTGAGCTTAAAAATTGGCTTGATAAT
GAAATTACATCACTTACCCACGAAGGCTTTATGAAAGAAGCTTATGAGAAAACTCTCGCCCCTATTTTTGGCGAGGAAAA
AATGGATTCTGTTATTTTCTCTCATTAA

Upstream 100 bases:

>100_bases
TTATGAAAGAGAACAAAGAATCTTTAATGATATTTACTTCTATCACATTTTTTTGTGGCACAATAATCTTTAATACCCAT
TTATAAAAGGAGTTAAATAA

Downstream 100 bases:

>100_bases
TTTTTACTCCATTGCTTAAAGTTTAAGCAATGGATTTTCATTCCTCCCATTTTTGTAATGCTAAATCTGTATTGATATGG
CAATACCCAAGTGCATTTTT

Product: amino-acid ABC transporter periplasmic solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKKLLLALFGVVFMIGLSACGDNQSTKESKNALEEIKQKGVVRIGVFSDKPPFGFINEKGQNDGFDVYIAKRIAKDLLGD
ENKVEFVLTEAASRVEFLRSNKVDIIMANFTQTPEREAVVDFAKPYMKVALGVISPNGEIKSIDDLKGKKLIVNKGTTAD
AFFSKNYPDIELLKYEQNTEAFLALKDKRGDALAHDNTLLLAWAMENEGFNVGIPTLGEEENIAPAIKKGNIELKNWLDN
EITSLTHEGFMKEAYEKTLAPIFGEEKMDSVIFSH

Sequences:

>Translated_275_residues
MKKLLLALFGVVFMIGLSACGDNQSTKESKNALEEIKQKGVVRIGVFSDKPPFGFINEKGQNDGFDVYIAKRIAKDLLGD
ENKVEFVLTEAASRVEFLRSNKVDIIMANFTQTPEREAVVDFAKPYMKVALGVISPNGEIKSIDDLKGKKLIVNKGTTAD
AFFSKNYPDIELLKYEQNTEAFLALKDKRGDALAHDNTLLLAWAMENEGFNVGIPTLGEEENIAPAIKKGNIELKNWLDN
EITSLTHEGFMKEAYEKTLAPIFGEEKMDSVIFSH
>Mature_275_residues
MKKLLLALFGVVFMIGLSACGDNQSTKESKNALEEIKQKGVVRIGVFSDKPPFGFINEKGQNDGFDVYIAKRIAKDLLGD
ENKVEFVLTEAASRVEFLRSNKVDIIMANFTQTPEREAVVDFAKPYMKVALGVISPNGEIKSIDDLKGKKLIVNKGTTAD
AFFSKNYPDIELLKYEQNTEAFLALKDKRGDALAHDNTLLLAWAMENEGFNVGIPTLGEEENIAPAIKKGNIELKNWLDN
EITSLTHEGFMKEAYEKTLAPIFGEEKMDSVIFSH

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=221, Percent_Identity=28.0542986425339, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787085, Length=255, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=25.5725190839695, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787031, Length=198, Percent_Identity=29.7979797979798, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=24.4635193133047, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30602; Mature: 30602

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLALFGVVFMIGLSACGDNQSTKESKNALEEIKQKGVVRIGVFSDKPPFGFINEKG
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
QNDGFDVYIAKRIAKDLLGDENKVEFVLTEAASRVEFLRSNKVDIIMANFTQTPEREAVV
CCCCEEHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
DFAKPYMKVALGVISPNGEIKSIDDLKGKKLIVNKGTTADAFFSKNYPDIELLKYEQNTE
HHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECCCCC
AFLALKDKRGDALAHDNTLLLAWAMENEGFNVGIPTLGEEENIAPAIKKGNIELKNWLDN
EEEEEECCCCCEEECCCEEEEEEEECCCCCEECCCCCCCCCCCCCHHHCCCEEHHHHHHH
EITSLTHEGFMKEAYEKTLAPIFGEEKMDSVIFSH
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MKKLLLALFGVVFMIGLSACGDNQSTKESKNALEEIKQKGVVRIGVFSDKPPFGFINEKG
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
QNDGFDVYIAKRIAKDLLGDENKVEFVLTEAASRVEFLRSNKVDIIMANFTQTPEREAVV
CCCCEEHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
DFAKPYMKVALGVISPNGEIKSIDDLKGKKLIVNKGTTADAFFSKNYPDIELLKYEQNTE
HHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHCCCCCCEEEEEECCCCC
AFLALKDKRGDALAHDNTLLLAWAMENEGFNVGIPTLGEEENIAPAIKKGNIELKNWLDN
EEEEEECCCCCEEECCCEEEEEEEECCCCCEECCCCCCCCCCCCCHHHCCCEEHHHHHHH
EITSLTHEGFMKEAYEKTLAPIFGEEKMDSVIFSH
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]