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The map label for this gene is dcuB

Identifier: 32266222

GI number: 32266222

Start: 703014

End: 704441

Strand: Direct

Name: dcuB

Synonym: HH0723

Alternate gene names: 32266222

Gene position: 703014-704441 (Clockwise)

Preceding gene: 32266221

Following gene: 32266223

Centisome position: 39.07

GC content: 42.65

Gene sequence:

>1428_bases
ATGGAAATAATATTACTGGTTTTACAAGTAGCAGTGCTACTTGGTGCGATTTTCTTAGGTATCCGACTAGGAGGTATGGC
GATAGGCTATGCTGGAGGATTCGGTGTTGTAGTGCTTTGTCTCGTGCTCGGTATGAAACCCGGAGATATTCCTTGGGACG
TGATTTTAATCATTATGTCTGTGATTGCAGCGATTGCGGCAATGCAACTTGCTGGTGGGCTAGACTATATGGTGCAAGTG
GCAGAAAAAATCTTGCGCAAGAATCCAAAATACATTAACTACCTTGCTCCAACGGTTACATATTTCCTTACATTCTTAGC
TGGCACTGGGCATACAGCATTTTCAATGATACCTGTGATTGTAGAAGTGGCAAAAGAGCAAAACATTAAACCTTCCGCAC
CTCTTTCAATCGCCGTTGTCGCAAGTCAGATTGCTATCACTGCATCGCCTGTAAGTGCTGCAGTGGTGTATATGAGTGGT
GCTCTTGAAGATTTTGGCTGGAATTACCCTACATTGCTACTTGTATGGCTTATTACAACTTTTGCCGCTTGTATGCTTAC
TGCATTTGTGGTGAGCAAGTTTTTCCCACTTGATTTAAGCAAGGACATCATCTATCAAGAACGATTAAAAGCGGGACTTG
TTAAGCCTTCTGTTGGTGCACAACATATTGAACTCAAAAAAGGTGCAAAACTAAGTGTAGGAATTTTCTTATTAGGTGTG
CTCTGCGTGGTGATTTATGCCACTTCAATTTCTGATGTTGTGCGTAAGCCTTTCTTGGATATCCTAAATGGTGTGATTGT
AAGCAATAATGGACAAGGGCTTATGGCAGGTATTGCTGAATTTATGAGAGGCTACATTGACCCAGTGAGATTACCACGCG
ATGGAGCGATTATGGCATTTATGTTAGCTATTGCTACTTGTATTACGATTTTTTGCAAGATAGATACAGGCAAACTTCTT
GAAGCCAGCACTTTTAAGGCAGGTATGACAGCTTGTATTTGTGTGCTAGGCGTTGCTTGGCTTGGCAATACCTTTGTCGC
CGGATACAAAAAAGAAATTGGAGATTTAGCAAGCAACCTTGTAGGTGATTATCCTGCACTTTTAGCAGTGGCACTTTTCT
TTGCAAGTATGTTGCTTTACTCTCAGGCAGCAACAGCAAAGGCTATTATGCCTGTGGTCATTGGTGCACTTGGCATAACT
GCGGCTACTCCAGAATCTTCCTACATTCTTGTAGCAAGCTTTGCAGCAGTTTCTGCTCTCTTCGTATTGCCCACATACCC
TACCCTGCTTGGTGCAGTACAAATGGACGATACCGGCTCAACGCGTATTGGTAAATATGTCTTTAACCATAGCTTTATTG
TGCCCGGCACACTTGCGATAATCTTTGCAGTAGGGCTTGGGTTTATAGTCACTCCTCTCTTTGCTTAA

Upstream 100 bases:

>100_bases
AAAAATTCGCCTTTTGCATAATCTCTTTGTAAAGTGTTTTTAGAGGAGTAGCCAAAGGCACTTCTTTAAAACTTACAATT
TCATTTTTTAAGGATTTTTT

Downstream 100 bases:

>100_bases
GATGTCTCTATATGCAGGAGCATTCAATGAATATCCTTGCGTATAGATTCTATTTTATTATTTATGTAAAATATATTGTT
TTTTCTATTTGTTGGTATTT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MEIILLVLQVAVLLGAIFLGIRLGGMAIGYAGGFGVVVLCLVLGMKPGDIPWDVILIIMSVIAAIAAMQLAGGLDYMVQV
AEKILRKNPKYINYLAPTVTYFLTFLAGTGHTAFSMIPVIVEVAKEQNIKPSAPLSIAVVASQIAITASPVSAAVVYMSG
ALEDFGWNYPTLLLVWLITTFAACMLTAFVVSKFFPLDLSKDIIYQERLKAGLVKPSVGAQHIELKKGAKLSVGIFLLGV
LCVVIYATSISDVVRKPFLDILNGVIVSNNGQGLMAGIAEFMRGYIDPVRLPRDGAIMAFMLAIATCITIFCKIDTGKLL
EASTFKAGMTACICVLGVAWLGNTFVAGYKKEIGDLASNLVGDYPALLAVALFFASMLLYSQAATAKAIMPVVIGALGIT
AATPESSYILVASFAAVSALFVLPTYPTLLGAVQMDDTGSTRIGKYVFNHSFIVPGTLAIIFAVGLGFIVTPLFA

Sequences:

>Translated_475_residues
MEIILLVLQVAVLLGAIFLGIRLGGMAIGYAGGFGVVVLCLVLGMKPGDIPWDVILIIMSVIAAIAAMQLAGGLDYMVQV
AEKILRKNPKYINYLAPTVTYFLTFLAGTGHTAFSMIPVIVEVAKEQNIKPSAPLSIAVVASQIAITASPVSAAVVYMSG
ALEDFGWNYPTLLLVWLITTFAACMLTAFVVSKFFPLDLSKDIIYQERLKAGLVKPSVGAQHIELKKGAKLSVGIFLLGV
LCVVIYATSISDVVRKPFLDILNGVIVSNNGQGLMAGIAEFMRGYIDPVRLPRDGAIMAFMLAIATCITIFCKIDTGKLL
EASTFKAGMTACICVLGVAWLGNTFVAGYKKEIGDLASNLVGDYPALLAVALFFASMLLYSQAATAKAIMPVVIGALGIT
AATPESSYILVASFAAVSALFVLPTYPTLLGAVQMDDTGSTRIGKYVFNHSFIVPGTLAIIFAVGLGFIVTPLFA
>Mature_475_residues
MEIILLVLQVAVLLGAIFLGIRLGGMAIGYAGGFGVVVLCLVLGMKPGDIPWDVILIIMSVIAAIAAMQLAGGLDYMVQV
AEKILRKNPKYINYLAPTVTYFLTFLAGTGHTAFSMIPVIVEVAKEQNIKPSAPLSIAVVASQIAITASPVSAAVVYMSG
ALEDFGWNYPTLLLVWLITTFAACMLTAFVVSKFFPLDLSKDIIYQERLKAGLVKPSVGAQHIELKKGAKLSVGIFLLGV
LCVVIYATSISDVVRKPFLDILNGVIVSNNGQGLMAGIAEFMRGYIDPVRLPRDGAIMAFMLAIATCITIFCKIDTGKLL
EASTFKAGMTACICVLGVAWLGNTFVAGYKKEIGDLASNLVGDYPALLAVALFFASMLLYSQAATAKAIMPVVIGALGIT
AATPESSYILVASFAAVSALFVLPTYPTLLGAVQMDDTGSTRIGKYVFNHSFIVPGTLAIIFAVGLGFIVTPLFA

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=469, Percent_Identity=53.7313432835821, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1790565, Length=443, Percent_Identity=33.6343115124154, Blast_Score=198, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 50207; Mature: 50207

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIILLVLQVAVLLGAIFLGIRLGGMAIGYAGGFGVVVLCLVLGMKPGDIPWDVILIIMS
CHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VIAAIAAMQLAGGLDYMVQVAEKILRKNPKYINYLAPTVTYFLTFLAGTGHTAFSMIPVI
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VEVAKEQNIKPSAPLSIAVVASQIAITASPVSAAVVYMSGALEDFGWNYPTLLLVWLITT
HHHHHHCCCCCCCCEEHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
FAACMLTAFVVSKFFPLDLSKDIIYQERLKAGLVKPSVGAQHIELKKGAKLSVGIFLLGV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHH
LCVVIYATSISDVVRKPFLDILNGVIVSNNGQGLMAGIAEFMRGYIDPVRLPRDGAIMAF
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHH
MLAIATCITIFCKIDTGKLLEASTFKAGMTACICVLGVAWLGNTFVAGYKKEIGDLASNL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGDYPALLAVALFFASMLLYSQAATAKAIMPVVIGALGITAATPESSYILVASFAAVSAL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHH
FVLPTYPTLLGAVQMDDTGSTRIGKYVFNHSFIVPGTLAIIFAVGLGFIVTPLFA
HHHCCHHHHHHEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIILLVLQVAVLLGAIFLGIRLGGMAIGYAGGFGVVVLCLVLGMKPGDIPWDVILIIMS
CHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VIAAIAAMQLAGGLDYMVQVAEKILRKNPKYINYLAPTVTYFLTFLAGTGHTAFSMIPVI
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VEVAKEQNIKPSAPLSIAVVASQIAITASPVSAAVVYMSGALEDFGWNYPTLLLVWLITT
HHHHHHCCCCCCCCEEHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
FAACMLTAFVVSKFFPLDLSKDIIYQERLKAGLVKPSVGAQHIELKKGAKLSVGIFLLGV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHH
LCVVIYATSISDVVRKPFLDILNGVIVSNNGQGLMAGIAEFMRGYIDPVRLPRDGAIMAF
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHH
MLAIATCITIFCKIDTGKLLEASTFKAGMTACICVLGVAWLGNTFVAGYKKEIGDLASNL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGDYPALLAVALFFASMLLYSQAATAKAIMPVVIGALGITAATPESSYILVASFAAVSAL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHH
FVLPTYPTLLGAVQMDDTGSTRIGKYVFNHSFIVPGTLAIIFAVGLGFIVTPLFA
HHHCCHHHHHHEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]