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The map label for this gene is frdB

Identifier: 32266186

GI number: 32266186

Start: 668019

End: 668762

Strand: Direct

Name: frdB

Synonym: HH0687

Alternate gene names: 32266186

Gene position: 668019-668762 (Clockwise)

Preceding gene: 32266185

Following gene: 32266187

Centisome position: 37.13

GC content: 40.32

Gene sequence:

>744_bases
ATGAGTGCAACACAACAAAAAGGAAGAACACTAACTATTCGAGCATTAAAGTTTGACCCACAAAGTGCAGTGTCGAAACC
ACATTTTCGTGAGTATCAGGTTGAAGAAGCTCATTCAATGACAGTTTTTATTGCATTGGGTATGATTAGAGAGCAACAAG
ACCCTGATTTAAGCTTTGATTTTGTATGTCGTGCTGGTATCTGCGGAAGCTGTGCAATGATGATTAACGGACGTCCAAGA
CTTGCGTGTAAAACACTTACTCAAGACTTCCCTGATGGTGTCATTACCCTTATGCCTCTCCCTGCATTTAAACTTATCAA
AGATTTAAGCGTAAATACAGGCGAATGGTTTGCAGGTATGACAAAACGAGTAGAAAGCTGGATACATACTCAACATCAGC
CCGATATTTCTAAACTTGAAGTGCCTATTGAACCTGAAGTAGCTGATGAAGTTTTTGAACTTGATAGATGTATTGAGTGT
GGCTGTTGTATTGCAAGCTGTGCAACAAAAGTTATGCGCGAAGATTTTGTTGGTGCAGCTGGAATGAATCGGATTGTGCG
ATTTATGATTGACCCGCACGATGAGCGCACTGATGAGGATTATTATGAGCTTGTAGGTAACGATGATGGTGTATTTGGTT
GTATGAGTCTTATTGCTTGCCACGATACTTGCCCAAAAGAACTTCCTCTACAGAGCAAAATTGCTTATTTGCGCCGCAAA
ATGCTTAGTGTAGGTATGAAATAA

Upstream 100 bases:

>100_bases
AAGCAAAGGGCGGTGATAGATATGCCTTGCAAGAAGCCTTGATGCCTTTCAATCTCCAACCACAATTTAAAGCAAGAAAC
CAACGACTAGGAGATAAGTA

Downstream 100 bases:

>100_bases
CACGATCTTTCTACCCTTTTACTTAAAAAGGGTAGATTCTAAAGAAACATAATGCAATCTATTTATTCTGTCTTTTTTTC
ATCAACAAATATTGCTTTGC

Product: fumarate reductase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSATQQKGRTLTIRALKFDPQSAVSKPHFREYQVEEAHSMTVFIALGMIREQQDPDLSFDFVCRAGICGSCAMMINGRPR
LACKTLTQDFPDGVITLMPLPAFKLIKDLSVNTGEWFAGMTKRVESWIHTQHQPDISKLEVPIEPEVADEVFELDRCIEC
GCCIASCATKVMREDFVGAAGMNRIVRFMIDPHDERTDEDYYELVGNDDGVFGCMSLIACHDTCPKELPLQSKIAYLRRK
MLSVGMK

Sequences:

>Translated_247_residues
MSATQQKGRTLTIRALKFDPQSAVSKPHFREYQVEEAHSMTVFIALGMIREQQDPDLSFDFVCRAGICGSCAMMINGRPR
LACKTLTQDFPDGVITLMPLPAFKLIKDLSVNTGEWFAGMTKRVESWIHTQHQPDISKLEVPIEPEVADEVFELDRCIEC
GCCIASCATKVMREDFVGAAGMNRIVRFMIDPHDERTDEDYYELVGNDDGVFGCMSLIACHDTCPKELPLQSKIAYLRRK
MLSVGMK
>Mature_246_residues
SATQQKGRTLTIRALKFDPQSAVSKPHFREYQVEEAHSMTVFIALGMIREQQDPDLSFDFVCRAGICGSCAMMINGRPRL
ACKTLTQDFPDGVITLMPLPAFKLIKDLSVNTGEWFAGMTKRVESWIHTQHQPDISKLEVPIEPEVADEVFELDRCIECG
CCIASCATKVMREDFVGAAGMNRIVRFMIDPHDERTDEDYYELVGNDDGVFGCMSLIACHDTCPKELPLQSKIAYLRRKM
LSVGMK

Specific function: The fumarate reductase enzyme complex is required for fumarate respiration using formate or sulfide as electron donor [H]

COG id: COG0479

COG function: function code C; Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI115387094, Length=233, Percent_Identity=31.7596566523605, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786943, Length=225, Percent_Identity=34.2222222222222, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790596, Length=220, Percent_Identity=28.6363636363636, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17533915, Length=232, Percent_Identity=30.1724137931034, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322987, Length=235, Percent_Identity=30.2127659574468, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137106, Length=236, Percent_Identity=29.6610169491525, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24643156, Length=251, Percent_Identity=29.4820717131474, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: NA

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 27796; Mature: 27664

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATQQKGRTLTIRALKFDPQSAVSKPHFREYQVEEAHSMTVFIALGMIREQQDPDLSFD
CCCCCCCCCEEEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
FVCRAGICGSCAMMINGRPRLACKTLTQDFPDGVITLMPLPAFKLIKDLSVNTGEWFAGM
HHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCHHHHHH
TKRVESWIHTQHQPDISKLEVPIEPEVADEVFELDRCIECGCCIASCATKVMREDFVGAA
HHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GMNRIVRFMIDPHDERTDEDYYELVGNDDGVFGCMSLIACHDTCPKELPLQSKIAYLRRK
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
MLSVGMK
HHHHCCC
>Mature Secondary Structure 
SATQQKGRTLTIRALKFDPQSAVSKPHFREYQVEEAHSMTVFIALGMIREQQDPDLSFD
CCCCCCCCEEEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
FVCRAGICGSCAMMINGRPRLACKTLTQDFPDGVITLMPLPAFKLIKDLSVNTGEWFAGM
HHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCHHHHHH
TKRVESWIHTQHQPDISKLEVPIEPEVADEVFELDRCIECGCCIASCATKVMREDFVGAA
HHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GMNRIVRFMIDPHDERTDEDYYELVGNDDGVFGCMSLIACHDTCPKELPLQSKIAYLRRK
HHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
MLSVGMK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2244791; 9492313; 14500908; 10586875; 11248702 [H]