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The map label for this gene is pyrC_2

Identifier: 32266113

GI number: 32266113

Start: 593645

End: 594676

Strand: Direct

Name: pyrC_2

Synonym: HH0614

Alternate gene names: 32266113

Gene position: 593645-594676 (Clockwise)

Preceding gene: 32266112

Following gene: 32266115

Centisome position: 33.0

GC content: 40.21

Gene sequence:

>1032_bases
ATGCCACAAACATTACAACTCACAAACCCTATGGATATGCATCTTCATTTGCGCGAGGGAGAAATGCTTAGTGCTATCTT
GCCTTTTAGTGCAAATCCTTTTAGTGCTGCTGTTGTTATGCCAAATCTTAAAACCCCTATTACCACTACTGCACAAGCTC
TTGCATACAAAAGGCAGATTCTATCACTTTGTTCTACATCATTTGAACCACTTATGAGTATTTTTCTTACTCCAGACCTT
GACAAACAAGAACTTATACGTGCTAAAGAAGCAGGCATTCATATTCTTAAACTTTATCCTAAGGGCTCAACTACGGGAAG
TGAGAAGGGCGTTAAAGAGATGTTATGCGAAAAGACACTTCATATCTTTGAGATTGCTCAAGAATTAGGCTTTATTCTCT
CCATACACGGCGAGAGTAATGGCTTTTGTATGGAAAGAGAATATGAATTTTTGCCTATTTTTGCCCATATTGCACAACAT
TTCCCTCGTCTTCGCATAATCATTGAGCATATGAGCGATAGGCGTAGTCTTGAGTTGATTGAACAATACCCAAATCTCTA
TGCTACACTTACATTACATCACATTACTATGAATCTTGATGACGTGCTTGGTGGGGGGATTAATCCTCATCACTTCTGCA
AACCTATGCTTAAAACCAAAAAAGACCAACAAGCCTTGCTGCAAGCTGCTCTAAATGCTCACCCAAAAGTAAGTTTTGGG
TCGGATTCTGCGCCTCATTTAGAATCTGCAAAACTTAACTCCAAAGGTGCAGCGGGGATTTTTTCTGCCCCTATTTTACT
CCCAGCCCTTGCTGAAGTTTTTGCATTTCATAACGCCCTTGATTGCTTGCAAACATTTATAAGCAATAGGGCTATCGCGA
ACTACAAGCTAAAGCATTTTCCGATTAAAACCATCACGCTTGAGCGTGTGGAAAATCCAGTGCCTCCTTACATTAATACA
CCTCTTGGGCATATTATACCACTCCGAGCGCAAACAAATCTCTCTTGGCGCATTAAAGAGGAAAATAGATGA

Upstream 100 bases:

>100_bases
TGTTACACGTTTTGAAGCTATAAGCAAAGAAGAATGCGAATCTTATAAAACTCATTTACTTGCTCTTCTGAAAAATTGTC
AAAAAACATAAGGACAAGCT

Downstream 100 bases:

>100_bases
GAAATAAATGCAGCACCTAAATCCTACATAAAATCCAAGTTGCAATCTCATCTGCCAAAAATAACTCATCTGCTACCAAA
AATAATTTGCCATTATAAGA

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MPQTLQLTNPMDMHLHLREGEMLSAILPFSANPFSAAVVMPNLKTPITTTAQALAYKRQILSLCSTSFEPLMSIFLTPDL
DKQELIRAKEAGIHILKLYPKGSTTGSEKGVKEMLCEKTLHIFEIAQELGFILSIHGESNGFCMEREYEFLPIFAHIAQH
FPRLRIIIEHMSDRRSLELIEQYPNLYATLTLHHITMNLDDVLGGGINPHHFCKPMLKTKKDQQALLQAALNAHPKVSFG
SDSAPHLESAKLNSKGAAGIFSAPILLPALAEVFAFHNALDCLQTFISNRAIANYKLKHFPIKTITLERVENPVPPYINT
PLGHIIPLRAQTNLSWRIKEENR

Sequences:

>Translated_343_residues
MPQTLQLTNPMDMHLHLREGEMLSAILPFSANPFSAAVVMPNLKTPITTTAQALAYKRQILSLCSTSFEPLMSIFLTPDL
DKQELIRAKEAGIHILKLYPKGSTTGSEKGVKEMLCEKTLHIFEIAQELGFILSIHGESNGFCMEREYEFLPIFAHIAQH
FPRLRIIIEHMSDRRSLELIEQYPNLYATLTLHHITMNLDDVLGGGINPHHFCKPMLKTKKDQQALLQAALNAHPKVSFG
SDSAPHLESAKLNSKGAAGIFSAPILLPALAEVFAFHNALDCLQTFISNRAIANYKLKHFPIKTITLERVENPVPPYINT
PLGHIIPLRAQTNLSWRIKEENR
>Mature_342_residues
PQTLQLTNPMDMHLHLREGEMLSAILPFSANPFSAAVVMPNLKTPITTTAQALAYKRQILSLCSTSFEPLMSIFLTPDLD
KQELIRAKEAGIHILKLYPKGSTTGSEKGVKEMLCEKTLHIFEIAQELGFILSIHGESNGFCMEREYEFLPIFAHIAQHF
PRLRIIIEHMSDRRSLELIEQYPNLYATLTLHHITMNLDDVLGGGINPHHFCKPMLKTKKDQQALLQAALNAHPKVSFGS
DSAPHLESAKLNSKGAAGIFSAPILLPALAEVFAFHNALDCLQTFISNRAIANYKLKHFPIKTITLERVENPVPPYINTP
LGHIIPLRAQTNLSWRIKEENR

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=348, Percent_Identity=33.6206896551724, Blast_Score=188, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323452, Length=370, Percent_Identity=34.8648648648649, Blast_Score=189, Evalue=6e-49,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_HELHP (Q7VIJ1)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860145.1
- HSSP:   P05020
- ProteinModelPortal:   Q7VIJ1
- SMR:   Q7VIJ1
- GeneID:   1493668
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0614
- NMPDR:   fig|235279.1.peg.614
- HOGENOM:   HBG628648
- OMA:   KESACGC
- ProtClustDB:   PRK05451
- BioCyc:   HHEP235279:HH_0614-MONOMER
- BRENDA:   3.5.2.3
- HAMAP:   MF_00219
- InterPro:   IPR006992
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF04909 Amidohydro_2

EC number: =3.5.2.3

Molecular weight: Translated: 38495; Mature: 38364

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTLQLTNPMDMHLHLREGEMLSAILPFSANPFSAAVVMPNLKTPITTTAQALAYKRQI
CCCCEECCCCCCEEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LSLCSTSFEPLMSIFLTPDLDKQELIRAKEAGIHILKLYPKGSTTGSEKGVKEMLCEKTL
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
HIFEIAQELGFILSIHGESNGFCMEREYEFLPIFAHIAQHFPRLRIIIEHMSDRRSLELI
HHHHHHHHCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHH
EQYPNLYATLTLHHITMNLDDVLGGGINPHHFCKPMLKTKKDQQALLQAALNAHPKVSFG
HHCCCCEEEEEEEEEEECHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
SDSAPHLESAKLNSKGAAGIFSAPILLPALAEVFAFHNALDCLQTFISNRAIANYKLKHF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEEC
PIKTITLERVENPVPPYINTPLGHIIPLRAQTNLSWRIKEENR
CEEEEEHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCC
>Mature Secondary Structure 
PQTLQLTNPMDMHLHLREGEMLSAILPFSANPFSAAVVMPNLKTPITTTAQALAYKRQI
CCCEECCCCCCEEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LSLCSTSFEPLMSIFLTPDLDKQELIRAKEAGIHILKLYPKGSTTGSEKGVKEMLCEKTL
HHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
HIFEIAQELGFILSIHGESNGFCMEREYEFLPIFAHIAQHFPRLRIIIEHMSDRRSLELI
HHHHHHHHCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHH
EQYPNLYATLTLHHITMNLDDVLGGGINPHHFCKPMLKTKKDQQALLQAALNAHPKVSFG
HHCCCCEEEEEEEEEEECHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
SDSAPHLESAKLNSKGAAGIFSAPILLPALAEVFAFHNALDCLQTFISNRAIANYKLKHF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEEC
PIKTITLERVENPVPPYINTPLGHIIPLRAQTNLSWRIKEENR
CEEEEEHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954