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The map label for this gene is yifB [H]

Identifier: 32266069

GI number: 32266069

Start: 548037

End: 549476

Strand: Direct

Name: yifB [H]

Synonym: HH0570

Alternate gene names: 32266069

Gene position: 548037-549476 (Clockwise)

Preceding gene: 32266068

Following gene: 32266070

Centisome position: 30.46

GC content: 38.12

Gene sequence:

>1440_bases
TTGCCTCATTTTAGTATCACCGGTTTAGCTCATAGCTCTATTCAAGAATCCAAACAACGTGTGCAATCCGCGCTTGCCAA
TAATGGCATTACTCTCCCGCCGCTTAAAATTAATATCAATCTCTCTCCATCTGACCTTCCTAAGAGTGGTGGGCATTTTG
ATTTGCCTATTGCGCTCGCATTGCAAAAACAAGGAGAGAATCTTGAACAATGGTTTGCTTTTGGCGAACTTGGGCTTGAT
GGAAGCATAAAATATCTTGATTCTATATATCCACTTTTGCTTGATATTTCCTTACTCCACCCTCACGCAAAGGTTATTGT
GCCTCTTTGCGCTAAAGAATTACTTAGTCTAATCCCCAACCTCACATTTTATTTTGCCTCGCATTTACAAGAAGCTATTG
AGCTTCTTAATAATCCGCAATTACATACAACTTCGCAACCTCAAATGACACATAACACACTTGGATTTAAGCATTTTGAA
CTTGAAGGAAAAACTTATTTTTATCAAGATGAGTTCGCATCTGATTTTAAACAAGTATGTGGGCAAGACATTGCCAAACG
CGCAGCACTCATTGCAGCAGCAGGATTCCATAATTTTATTATGGAAGGCTCACCGGGCTGTGGCAAAAGTATGATTGCCA
AACGACTTTTATACATTTTACCCCCTAGCTCTATGCAGGAAATGATAGAAAATGTCAAATCTCAAGCGCTCAATAAGCAA
AGCACTCATTATACACCTTTGCGTCCATTTCGTAATCCTCACCAAAGTGCGAGTAAATCAAGTATTCTTGGTTCAGCTAC
GCAGTTTGAAGTTAAACCAGGTGAGGTTGCTTTAGCACATAATGGCATTTTATTTTTTGATGAATTACCACATTTTAAAA
AAGATATTTTAGAATCTTTGCGTGAGCCACTTGAAAACAATAAGCTTGCTATTTCCCGTGTGCATTCTAAGCTTGAATAT
GACACAAATTTTATGTTTGTAGGTGCGCTTAATCCTTGTCCTTGCGGCAATCTTCTCAGCAAAAATAAACAATGTCGCTG
TCAAGAAAGAGAAATTAATCTCTATCGCTCAAAGCTTTCTGAACCTTTCCTTGATAGAATTGACCTTTTTGTCCAAATGA
GTGAGCAAGAAGACAGGCAAACACAAGCAGGACTAGATTCTCTAAGTATGCAAAAAATGGTTTTTGATGCTTTTAAAATG
CAAAAAAAACGAAAGCAAGAAGTGTTTAATGGCAAACTTGATGAGGCAGAAATTGAGCAATTTTGCATATTAGATATAGA
TTCTAAGAATCTACTTGACCAAGCGAGTGAGCGTTTTTCTCTCTCTTATCGCGCACAAAATAAGCTCAAAAAAGTAGCGC
GCACTATTGCGGATTTAGCCAAAGAGGAGCATATCACTAAAGCTCATTTGCTTGAAGCCCTAAGTTATCGGCGTATTTAA

Upstream 100 bases:

>100_bases
TCAAGCCTAAGAATTGGTAATGGTAAATACAATCCTAAGTGCAACAAGCTTCGGCACATCAGCAAAAGTAGTTGAAGTAG
AAGCAACTTTTACAAGAGGA

Downstream 100 bases:

>100_bases
AGAATCTCAGCACAAGGAGAAAAAATGCTTGAACATAAAACGATGCAAAATATACATAAACGGCTTAAAAAAATTATTGG
ACAGCTTAATGGTATTGATA

Product: competence protein ComM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MPHFSITGLAHSSIQESKQRVQSALANNGITLPPLKININLSPSDLPKSGGHFDLPIALALQKQGENLEQWFAFGELGLD
GSIKYLDSIYPLLLDISLLHPHAKVIVPLCAKELLSLIPNLTFYFASHLQEAIELLNNPQLHTTSQPQMTHNTLGFKHFE
LEGKTYFYQDEFASDFKQVCGQDIAKRAALIAAAGFHNFIMEGSPGCGKSMIAKRLLYILPPSSMQEMIENVKSQALNKQ
STHYTPLRPFRNPHQSASKSSILGSATQFEVKPGEVALAHNGILFFDELPHFKKDILESLREPLENNKLAISRVHSKLEY
DTNFMFVGALNPCPCGNLLSKNKQCRCQEREINLYRSKLSEPFLDRIDLFVQMSEQEDRQTQAGLDSLSMQKMVFDAFKM
QKKRKQEVFNGKLDEAEIEQFCILDIDSKNLLDQASERFSLSYRAQNKLKKVARTIADLAKEEHITKAHLLEALSYRRI

Sequences:

>Translated_479_residues
MPHFSITGLAHSSIQESKQRVQSALANNGITLPPLKININLSPSDLPKSGGHFDLPIALALQKQGENLEQWFAFGELGLD
GSIKYLDSIYPLLLDISLLHPHAKVIVPLCAKELLSLIPNLTFYFASHLQEAIELLNNPQLHTTSQPQMTHNTLGFKHFE
LEGKTYFYQDEFASDFKQVCGQDIAKRAALIAAAGFHNFIMEGSPGCGKSMIAKRLLYILPPSSMQEMIENVKSQALNKQ
STHYTPLRPFRNPHQSASKSSILGSATQFEVKPGEVALAHNGILFFDELPHFKKDILESLREPLENNKLAISRVHSKLEY
DTNFMFVGALNPCPCGNLLSKNKQCRCQEREINLYRSKLSEPFLDRIDLFVQMSEQEDRQTQAGLDSLSMQKMVFDAFKM
QKKRKQEVFNGKLDEAEIEQFCILDIDSKNLLDQASERFSLSYRAQNKLKKVARTIADLAKEEHITKAHLLEALSYRRI
>Mature_478_residues
PHFSITGLAHSSIQESKQRVQSALANNGITLPPLKININLSPSDLPKSGGHFDLPIALALQKQGENLEQWFAFGELGLDG
SIKYLDSIYPLLLDISLLHPHAKVIVPLCAKELLSLIPNLTFYFASHLQEAIELLNNPQLHTTSQPQMTHNTLGFKHFEL
EGKTYFYQDEFASDFKQVCGQDIAKRAALIAAAGFHNFIMEGSPGCGKSMIAKRLLYILPPSSMQEMIENVKSQALNKQS
THYTPLRPFRNPHQSASKSSILGSATQFEVKPGEVALAHNGILFFDELPHFKKDILESLREPLENNKLAISRVHSKLEYD
TNFMFVGALNPCPCGNLLSKNKQCRCQEREINLYRSKLSEPFLDRIDLFVQMSEQEDRQTQAGLDSLSMQKMVFDAFKMQ
KKRKQEVFNGKLDEAEIEQFCILDIDSKNLLDQASERFSLSYRAQNKLKKVARTIADLAKEEHITKAHLLEALSYRRI

Specific function: Unknown

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=495, Percent_Identity=35.1515151515151, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 54145; Mature: 54014

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00676 SIGMA54_INTERACT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFSITGLAHSSIQESKQRVQSALANNGITLPPLKININLSPSDLPKSGGHFDLPIALA
CCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHEEE
LQKQGENLEQWFAFGELGLDGSIKYLDSIYPLLLDISLLHPHAKVIVPLCAKELLSLIPN
EHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCH
LTFYFASHLQEAIELLNNPQLHTTSQPQMTHNTLGFKHFELEGKTYFYQDEFASDFKQVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCEEEEHHHHHHHHHHHH
GQDIAKRAALIAAAGFHNFIMEGSPGCGKSMIAKRLLYILPPSSMQEMIENVKSQALNKQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
STHYTPLRPFRNPHQSASKSSILGSATQFEVKPGEVALAHNGILFFDELPHFKKDILESL
CCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEEECCEEEECCCCHHHHHHHHHH
REPLENNKLAISRVHSKLEYDTNFMFVGALNPCPCGNLLSKNKQCRCQEREINLYRSKLS
HHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHC
EPFLDRIDLFVQMSEQEDRQTQAGLDSLSMQKMVFDAFKMQKKRKQEVFNGKLDEAEIEQ
CHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
FCILDIDSKNLLDQASERFSLSYRAQNKLKKVARTIADLAKEEHITKAHLLEALSYRRI
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PHFSITGLAHSSIQESKQRVQSALANNGITLPPLKININLSPSDLPKSGGHFDLPIALA
CCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHEEE
LQKQGENLEQWFAFGELGLDGSIKYLDSIYPLLLDISLLHPHAKVIVPLCAKELLSLIPN
EHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCH
LTFYFASHLQEAIELLNNPQLHTTSQPQMTHNTLGFKHFELEGKTYFYQDEFASDFKQVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCEEEEHHHHHHHHHHHH
GQDIAKRAALIAAAGFHNFIMEGSPGCGKSMIAKRLLYILPPSSMQEMIENVKSQALNKQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
STHYTPLRPFRNPHQSASKSSILGSATQFEVKPGEVALAHNGILFFDELPHFKKDILESL
CCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEEECCEEEECCCCHHHHHHHHHH
REPLENNKLAISRVHSKLEYDTNFMFVGALNPCPCGNLLSKNKQCRCQEREINLYRSKLS
HHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHC
EPFLDRIDLFVQMSEQEDRQTQAGLDSLSMQKMVFDAFKMQKKRKQEVFNGKLDEAEIEQ
CHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
FCILDIDSKNLLDQASERFSLSYRAQNKLKKVARTIADLAKEEHITKAHLLEALSYRRI
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]