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The map label for this gene is def

Identifier: 32266068

GI number: 32266068

Start: 547401

End: 547946

Strand: Direct

Name: def

Synonym: HH0569

Alternate gene names: 32266068

Gene position: 547401-547946 (Clockwise)

Preceding gene: 32266067

Following gene: 32266069

Centisome position: 30.43

GC content: 34.8

Gene sequence:

>546_bases
TTGAAATACCCGGAGATGACTTTGGCGACCTTAGCGATTTTGAAATATCCTAATGCACTATTGCGCAAAAAATCTATACC
TGTTGAGATTTTTGATGATAATTTACATAACTTCCTTGATGATATGTATGAGACGCTTATAGAAAGCAAAGGTGTTGGCT
TAGCTGCTATTCAAGTAGGAAGGGCGGAGCGAATTCTTATTATTAATATTCCGCGTGAAGAGGATAAACAACAATATAAA
GAAGATTTGCTTGAAATCATTAATCCCACTTTTTTAACGCAAGAAGAATGTGTAGAATGGGAAGAGGGCTGTTTATCTGT
GCCTGATTTTTATGAAAGTATCAAACGATTTGATAAAGTCTCAATCGCTTATAAAGACCGCTATGGTAATGACAGGATTC
TCAAAGCACAAGGATTCCTTGCGGTAGCAATTCAACACGAAATAGACCACCTCAATGGTGTTTTATTTGTAGATAAACTC
CCTATTTTAAAACGCAAAAAATTTGAAAAAGAACTCAAAAAACTTAAAAAAGAATCTCAAGCCTAA

Upstream 100 bases:

>100_bases
TTAGAAGAAGCCAAAAAAATGCTTGATGAAGCTTTAGCAGAAGATAAACCTCTTAAATTTGGCAAAACAGGGGCAGGTAG
CTCTACAAATAATGATGACT

Downstream 100 bases:

>100_bases
GAATTGGTAATGGTAAATACAATCCTAAGTGCAACAAGCTTCGGCACATCAGCAAAAGTAGTTGAAGTAGAAGCAACTTT
TACAAGAGGATTGCCTCATT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKYPEMTLATLAILKYPNALLRKKSIPVEIFDDNLHNFLDDMYETLIESKGVGLAAIQVGRAERILIINIPREEDKQQYK
EDLLEIINPTFLTQEECVEWEEGCLSVPDFYESIKRFDKVSIAYKDRYGNDRILKAQGFLAVAIQHEIDHLNGVLFVDKL
PILKRKKFEKELKKLKKESQA

Sequences:

>Translated_181_residues
MKYPEMTLATLAILKYPNALLRKKSIPVEIFDDNLHNFLDDMYETLIESKGVGLAAIQVGRAERILIINIPREEDKQQYK
EDLLEIINPTFLTQEECVEWEEGCLSVPDFYESIKRFDKVSIAYKDRYGNDRILKAQGFLAVAIQHEIDHLNGVLFVDKL
PILKRKKFEKELKKLKKESQA
>Mature_181_residues
MKYPEMTLATLAILKYPNALLRKKSIPVEIFDDNLHNFLDDMYETLIESKGVGLAAIQVGRAERILIINIPREEDKQQYK
EDLLEIINPTFLTQEECVEWEEGCLSVPDFYESIKRFDKVSIAYKDRYGNDRILKAQGFLAVAIQHEIDHLNGVLFVDKL
PILKRKKFEKELKKLKKESQA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=38.2352941176471, Blast_Score=118, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645728, Length=152, Percent_Identity=30.2631578947368, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_HELHP (Q7VIN5)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860100.1
- ProteinModelPortal:   Q7VIN5
- SMR:   Q7VIN5
- GeneID:   1493623
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0569
- NMPDR:   fig|235279.1.peg.569
- HOGENOM:   HBG665227
- OMA:   EFLAIVM
- ProtClustDB:   PRK00150
- BioCyc:   HHEP235279:HH_0569-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21073; Mature: 21073

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: ACT_SITE 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYPEMTLATLAILKYPNALLRKKSIPVEIFDDNLHNFLDDMYETLIESKGVGLAAIQVG
CCCCHHHHHHHHHHHCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEC
RAERILIINIPREEDKQQYKEDLLEIINPTFLTQEECVEWEEGCLSVPDFYESIKRFDKV
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
SIAYKDRYGNDRILKAQGFLAVAIQHEIDHLNGVLFVDKLPILKRKKFEKELKKLKKESQ
HEEEECCCCCCEEEEECCEEEEEEHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MKYPEMTLATLAILKYPNALLRKKSIPVEIFDDNLHNFLDDMYETLIESKGVGLAAIQVG
CCCCHHHHHHHHHHHCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEC
RAERILIINIPREEDKQQYKEDLLEIINPTFLTQEECVEWEEGCLSVPDFYESIKRFDKV
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
SIAYKDRYGNDRILKAQGFLAVAIQHEIDHLNGVLFVDKLPILKRKKFEKELKKLKKESQ
HEEEECCCCCCEEEEECCEEEEEEHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954