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The map label for this gene is dcuA

Identifier: 32265878

GI number: 32265878

Start: 370065

End: 371639

Strand: Direct

Name: dcuA

Synonym: HH0379

Alternate gene names: 32265878

Gene position: 370065-371639 (Clockwise)

Preceding gene: 32265876

Following gene: 32265879

Centisome position: 20.57

GC content: 38.98

Gene sequence:

>1575_bases
ATGGAATTTTTTACAAGTTTAAATGAAGGGTGGCAATTTAGTATTCAGCTCCTTGTCGTGCTTATTTGCCTTTTTTATGG
TGCAAGAAAAGGCGGTATTGCTTTGGGATTACTTGGGGGCATTGGTATTTTAATGCTCGTATTTGTCTTTCATATCAAGC
CGGGCAAACCTGCTATTGATGTTATGCTTACCATTCTTGCTGTGGTTGTAGCAAGTGCAACACTACAAGCAAGCGGAGGC
TTAGATGTGATGCTCCAAATTGCAGAGCGTATTCTAAGACGCAATCCGAAATTTCTCACAATCCTTGCTCCTTTTGTAAC
TTGCTTTTTGACGATTCTTTGTGGCACAGGACACGTAGTTTATACAATTATGCCTATTATTTATGATATTGCAATTAAAA
ACAATATCCGCCCTGAACGACCTATGGCAGCTGCTTCTATTTCTTCACAAATGGGGATTATTGCATCTCCTGTATCTGTG
GCAGTTGTGAGCCTAACAGCCTTGCTTTTAAATACAGAGCATAAACTTGCAGGATTTGATGGCTATATCAATTTGCTTCA
AATTACTATTCCTAGCACACTTTTTGGCGTGCTTTGCATCGGTATTTTTTCTTGGTTTAGAGGCAAAGACTTGGATAAAG
ACTTGGAATTTCAAGAAAAGTTAAAAGACCCCGAATTTCACAAATATGTTTATGGGGATTCTAAAACACTTTTGGGAGTC
AAACTTCCCACAAAAGATTGGGTGGCTATGTGGATATTTTTAGGTGCAATCGCACTTGTAGCCTTAGTAGGAGCTTTTGA
ATCTATCAAGCCAAACTGGGGACAAAAAATGGCTTATGAAGCTCATCTGCAAGAAACAACTATCAAATGGACACCAAATG
GTGAGAATAAAGGTATAGTGGATTTAAAGGCTCTAGGCTTTGACCAAAAGGCACAAGGGGGGGGGCAGAGTGCATTTAAA
TCCGCTTTAGAATCTGGAGAAATTGTAGCTATTGCACAAAAAGACAAGCTTGGTAATCCATCTATGGATTATATTTCAAT
GGTAAATGTCATTCAAGCTTTTATGCTCCTCGCAGGCGCACTCATTATTATTTTTACGAATGTTGATGCGAAAAAAATTG
GGAATAATGAAATTTTTAAATCTGGTATGATTGCGCTCGTAGCAGTATTTGGAATCTCGTGGATGGCAGATACAATGTTT
GCAGTGCATACACCGATGATGAAAGATGCACTTGGTGATATTGTCAAAGCTCACCCTTGGACTTATGCGATTATGCTCTT
GCTGATTTCTAAATTTGTTAATTCTCAAGCAGCAGCTATTGCAGCATTTGTGCCCTTGGCTTTGGGTATTGGTGTAGAGC
CGGGTATTATTGTAGCTTTTGCGGCAGCTTGTTATGGATATTATATTTTGCCTACTTATCCTAGTGATTTGGCAACGATT
CAATTTGATAGAAGTGGCACAACGCATATTGGCAGATTTGTTATTAATCACAGCTTTATCCTACCAGGGCTTATTGGGGT
ATTTAGCTCTTGTGTAGCAGGATATTTGCTTGCAAAGGTTGCAGGATATATTTAG

Upstream 100 bases:

>100_bases
TCACGCCATAAAAAAACTTAAATATTATCTATGATTAAGTCAGATTTAACATTCTTTTAGAAATAATGCTCCAAATTTTT
CTCATACTCAAGGAGGACTT

Downstream 100 bases:

>100_bases
CTTAACTTGATAGAATCTAAATGCGGATTCTATCAAGTTTAAGGAGCATATATGCCTTATCTTTTGCTTACAGGTGCAAG
TGGCTACATCGGTTCTCATA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MEFFTSLNEGWQFSIQLLVVLICLFYGARKGGIALGLLGGIGILMLVFVFHIKPGKPAIDVMLTILAVVVASATLQASGG
LDVMLQIAERILRRNPKFLTILAPFVTCFLTILCGTGHVVYTIMPIIYDIAIKNNIRPERPMAAASISSQMGIIASPVSV
AVVSLTALLLNTEHKLAGFDGYINLLQITIPSTLFGVLCIGIFSWFRGKDLDKDLEFQEKLKDPEFHKYVYGDSKTLLGV
KLPTKDWVAMWIFLGAIALVALVGAFESIKPNWGQKMAYEAHLQETTIKWTPNGENKGIVDLKALGFDQKAQGGGQSAFK
SALESGEIVAIAQKDKLGNPSMDYISMVNVIQAFMLLAGALIIIFTNVDAKKIGNNEIFKSGMIALVAVFGISWMADTMF
AVHTPMMKDALGDIVKAHPWTYAIMLLLISKFVNSQAAAIAAFVPLALGIGVEPGIIVAFAAACYGYYILPTYPSDLATI
QFDRSGTTHIGRFVINHSFILPGLIGVFSSCVAGYLLAKVAGYI

Sequences:

>Translated_524_residues
MEFFTSLNEGWQFSIQLLVVLICLFYGARKGGIALGLLGGIGILMLVFVFHIKPGKPAIDVMLTILAVVVASATLQASGG
LDVMLQIAERILRRNPKFLTILAPFVTCFLTILCGTGHVVYTIMPIIYDIAIKNNIRPERPMAAASISSQMGIIASPVSV
AVVSLTALLLNTEHKLAGFDGYINLLQITIPSTLFGVLCIGIFSWFRGKDLDKDLEFQEKLKDPEFHKYVYGDSKTLLGV
KLPTKDWVAMWIFLGAIALVALVGAFESIKPNWGQKMAYEAHLQETTIKWTPNGENKGIVDLKALGFDQKAQGGGQSAFK
SALESGEIVAIAQKDKLGNPSMDYISMVNVIQAFMLLAGALIIIFTNVDAKKIGNNEIFKSGMIALVAVFGISWMADTMF
AVHTPMMKDALGDIVKAHPWTYAIMLLLISKFVNSQAAAIAAFVPLALGIGVEPGIIVAFAAACYGYYILPTYPSDLATI
QFDRSGTTHIGRFVINHSFILPGLIGVFSSCVAGYLLAKVAGYI
>Mature_524_residues
MEFFTSLNEGWQFSIQLLVVLICLFYGARKGGIALGLLGGIGILMLVFVFHIKPGKPAIDVMLTILAVVVASATLQASGG
LDVMLQIAERILRRNPKFLTILAPFVTCFLTILCGTGHVVYTIMPIIYDIAIKNNIRPERPMAAASISSQMGIIASPVSV
AVVSLTALLLNTEHKLAGFDGYINLLQITIPSTLFGVLCIGIFSWFRGKDLDKDLEFQEKLKDPEFHKYVYGDSKTLLGV
KLPTKDWVAMWIFLGAIALVALVGAFESIKPNWGQKMAYEAHLQETTIKWTPNGENKGIVDLKALGFDQKAQGGGQSAFK
SALESGEIVAIAQKDKLGNPSMDYISMVNVIQAFMLLAGALIIIFTNVDAKKIGNNEIFKSGMIALVAVFGISWMADTMF
AVHTPMMKDALGDIVKAHPWTYAIMLLLISKFVNSQAAAIAAFVPLALGIGVEPGIIVAFAAACYGYYILPTYPSDLATI
QFDRSGTTHIGRFVINHSFILPGLIGVFSSCVAGYLLAKVAGYI

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=504, Percent_Identity=54.7619047619048, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1790580, Length=210, Percent_Identity=39.0476190476191, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 56703; Mature: 56703

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFFTSLNEGWQFSIQLLVVLICLFYGARKGGIALGLLGGIGILMLVFVFHIKPGKPAID
CCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHH
VMLTILAVVVASATLQASGGLDVMLQIAERILRRNPKFLTILAPFVTCFLTILCGTGHVV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHH
YTIMPIIYDIAIKNNIRPERPMAAASISSQMGIIASPVSVAVVSLTALLLNTEHKLAGFD
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCC
GYINLLQITIPSTLFGVLCIGIFSWFRGKDLDKDLEFQEKLKDPEFHKYVYGDSKTLLGV
HHEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHEEEECCCCEEEEE
KLPTKDWVAMWIFLGAIALVALVGAFESIKPNWGQKMAYEAHLQETTIKWTPNGENKGIV
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEECCCCCCCCEE
DLKALGFDQKAQGGGQSAFKSALESGEIVAIAQKDKLGNPSMDYISMVNVIQAFMLLAGA
EEEECCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
LIIIFTNVDAKKIGNNEIFKSGMIALVAVFGISWMADTMFAVHTPMMKDALGDIVKAHPW
CEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCH
TYAIMLLLISKFVNSQAAAIAAFVPLALGIGVEPGIIVAFAAACYGYYILPTYPSDLATI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCCCCCEEE
QFDRSGTTHIGRFVINHSFILPGLIGVFSSCVAGYLLAKVAGYI
EECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFFTSLNEGWQFSIQLLVVLICLFYGARKGGIALGLLGGIGILMLVFVFHIKPGKPAID
CCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHH
VMLTILAVVVASATLQASGGLDVMLQIAERILRRNPKFLTILAPFVTCFLTILCGTGHVV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHH
YTIMPIIYDIAIKNNIRPERPMAAASISSQMGIIASPVSVAVVSLTALLLNTEHKLAGFD
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCC
GYINLLQITIPSTLFGVLCIGIFSWFRGKDLDKDLEFQEKLKDPEFHKYVYGDSKTLLGV
HHEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHEEEECCCCEEEEE
KLPTKDWVAMWIFLGAIALVALVGAFESIKPNWGQKMAYEAHLQETTIKWTPNGENKGIV
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEECCCCCCCCEE
DLKALGFDQKAQGGGQSAFKSALESGEIVAIAQKDKLGNPSMDYISMVNVIQAFMLLAGA
EEEECCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
LIIIFTNVDAKKIGNNEIFKSGMIALVAVFGISWMADTMFAVHTPMMKDALGDIVKAHPW
CEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCH
TYAIMLLLISKFVNSQAAAIAAFVPLALGIGVEPGIIVAFAAACYGYYILPTYPSDLATI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCCCCCEEE
QFDRSGTTHIGRFVINHSFILPGLIGVFSSCVAGYLLAKVAGYI
EECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]