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The map label for this gene is hyaA

Identifier: 32265555

GI number: 32265555

Start: 60643

End: 61803

Strand: Direct

Name: hyaA

Synonym: HH0056

Alternate gene names: 32265555

Gene position: 60643-61803 (Clockwise)

Preceding gene: 32265552

Following gene: 32265556

Centisome position: 3.37

GC content: 42.64

Gene sequence:

>1161_bases
ATGAAAACACAAGATGCATACCAAGAGTTGTTTAAAAAGGCTTCCCAAAGGCTTGATGAGCTCTCCAAGCTTCCTGCTCA
TAATGCAGAAAAAGACTTATTACAAGAATTAGAATCTAAAGGATTCTCTCGCCGTGATTTTATGAAATGGTCAGGAATGA
TGACAGCTGCATTAGCCTTACCCGCAACTTTTGCCCCGCTCACTGCTAAAGCTGCAGAAGTTGCTAATCGTCTCCCTGTG
GTATGGTTACATATGGCAGAATGCACAGGTTGCAGTGAAAGCTTACTTCGCAGTGATGCGCCGAGCATAGATTCTTTAAT
CTTTGATTATATAAGCCTTGAATACCACGAAACCGTTATGGCTGCTTCAGGTTGGCAAGCCGAACATAATCTTGAAAATG
CCATCGAAAAATATGCTGGGCAATATATTTTAATGGTAGAAGGCGGTATCCCCAAAGGCAGTAGTGAGTTTTATCTCACT
ATCGGAGGACACGCTCGCACAGGTGCAGAAGAAGCACGACACGCAGCAAAAGGCGCAAAAGCAATTTTTGCCATTGGCAC
TTGTTCAAGCTTTGGCGGTGTCCAAGCTGCACATCCTAACCCAACAAATGCTGTGCCTCTTAGCGAAATTACTAACCGCA
GTGTCATTAATGTGCCTGGTTGTCCGCCAAGTGAAAAAAACATTGTAGGAAATGTGTTGCATTTTATCCTTTTTGGCTCA
CTTCCTTCACTTGATGCATATAATCGTCCAACTTGGGCTTATGGACTAAGAATCCACGATTTATGCGAACGTCGAGGACG
TTTTGATGCAGGACAATTCGTTCAACATTTTGGCGATGAAAATGCACAAAAAGGCTACTGCCTTTACAAAGTAGGCTGTA
AAGGACCTTATACTTTTAACAATTGCTCAAAATTAAGATTTAACCAACATACAAGTTGGCCTATTCAAGCAGGACACGGC
TGTATAGGTTGTAGTGAGCCTCACTTTTGGGATACAATGAACCCATTTGAAGAGCCTATAGGCAATCGTCTTTATGCAAC
AAGTTTTAATGGAATGGGCGCAGACAAAACGGCAGATACAATCGGTGCTGTAGTGCTTGGGGCTGCAGCCATAGGTATTG
CCGCACACGCAATTATTAGTTCAACACAAAATGCAAAATAA

Upstream 100 bases:

>100_bases
TAAAACAATCAAAGCATTTTATAAACAATTATAAACAAAATCTCAAATTTAATGTAAAATACTATTGCTTAACAACAAAA
ATCATAAGGAAAGGGTTTTT

Downstream 100 bases:

>100_bases
GGGGTAGAATATGACAAAACGTATTATTGTAGATCCAATTACAAGAATTGAAGGGCACTTACGAATTGAGGTTATTGTTG
ATGAAAATAATGTGATACAA

Product: Ni/Fe hydrogenase beta subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKTQDAYQELFKKASQRLDELSKLPAHNAEKDLLQELESKGFSRRDFMKWSGMMTAALALPATFAPLTAKAAEVANRLPV
VWLHMAECTGCSESLLRSDAPSIDSLIFDYISLEYHETVMAASGWQAEHNLENAIEKYAGQYILMVEGGIPKGSSEFYLT
IGGHARTGAEEARHAAKGAKAIFAIGTCSSFGGVQAAHPNPTNAVPLSEITNRSVINVPGCPPSEKNIVGNVLHFILFGS
LPSLDAYNRPTWAYGLRIHDLCERRGRFDAGQFVQHFGDENAQKGYCLYKVGCKGPYTFNNCSKLRFNQHTSWPIQAGHG
CIGCSEPHFWDTMNPFEEPIGNRLYATSFNGMGADKTADTIGAVVLGAAAIGIAAHAIISSTQNAK

Sequences:

>Translated_386_residues
MKTQDAYQELFKKASQRLDELSKLPAHNAEKDLLQELESKGFSRRDFMKWSGMMTAALALPATFAPLTAKAAEVANRLPV
VWLHMAECTGCSESLLRSDAPSIDSLIFDYISLEYHETVMAASGWQAEHNLENAIEKYAGQYILMVEGGIPKGSSEFYLT
IGGHARTGAEEARHAAKGAKAIFAIGTCSSFGGVQAAHPNPTNAVPLSEITNRSVINVPGCPPSEKNIVGNVLHFILFGS
LPSLDAYNRPTWAYGLRIHDLCERRGRFDAGQFVQHFGDENAQKGYCLYKVGCKGPYTFNNCSKLRFNQHTSWPIQAGHG
CIGCSEPHFWDTMNPFEEPIGNRLYATSFNGMGADKTADTIGAVVLGAAAIGIAAHAIISSTQNAK
>Mature_386_residues
MKTQDAYQELFKKASQRLDELSKLPAHNAEKDLLQELESKGFSRRDFMKWSGMMTAALALPATFAPLTAKAAEVANRLPV
VWLHMAECTGCSESLLRSDAPSIDSLIFDYISLEYHETVMAASGWQAEHNLENAIEKYAGQYILMVEGGIPKGSSEFYLT
IGGHARTGAEEARHAAKGAKAIFAIGTCSSFGGVQAAHPNPTNAVPLSEITNRSVINVPGCPPSEKNIVGNVLHFILFGS
LPSLDAYNRPTWAYGLRIHDLCERRGRFDAGQFVQHFGDENAQKGYCLYKVGCKGPYTFNNCSKLRFNQHTSWPIQAGHG
CIGCSEPHFWDTMNPFEEPIGNRLYATSFNGMGADKTADTIGAVVLGAAAIGIAAHAIISSTQNAK

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=341, Percent_Identity=45.4545454545455, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=48.2876712328767, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 41966; Mature: 41966

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQDAYQELFKKASQRLDELSKLPAHNAEKDLLQELESKGFSRRDFMKWSGMMTAALAL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
PATFAPLTAKAAEVANRLPVVWLHMAECTGCSESLLRSDAPSIDSLIFDYISLEYHETVM
CHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AASGWQAEHNLENAIEKYAGQYILMVEGGIPKGSSEFYLTIGGHARTGAEEARHAAKGAK
HCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCC
AIFAIGTCSSFGGVQAAHPNPTNAVPLSEITNRSVINVPGCPPSEKNIVGNVLHFILFGS
EEEEEECCCCCCCEECCCCCCCCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCC
LPSLDAYNRPTWAYGLRIHDLCERRGRFDAGQFVQHFGDENAQKGYCLYKVGCKGPYTFN
CCCCCCCCCCCCEECCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
NCSKLRFNQHTSWPIQAGHGCIGCSEPHFWDTMNPFEEPIGNRLYATSFNGMGADKTADT
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHH
IGAVVLGAAAIGIAAHAIISSTQNAK
HHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKTQDAYQELFKKASQRLDELSKLPAHNAEKDLLQELESKGFSRRDFMKWSGMMTAALAL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
PATFAPLTAKAAEVANRLPVVWLHMAECTGCSESLLRSDAPSIDSLIFDYISLEYHETVM
CHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AASGWQAEHNLENAIEKYAGQYILMVEGGIPKGSSEFYLTIGGHARTGAEEARHAAKGAK
HCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCC
AIFAIGTCSSFGGVQAAHPNPTNAVPLSEITNRSVINVPGCPPSEKNIVGNVLHFILFGS
EEEEEECCCCCCCEECCCCCCCCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCC
LPSLDAYNRPTWAYGLRIHDLCERRGRFDAGQFVQHFGDENAQKGYCLYKVGCKGPYTFN
CCCCCCCCCCCCEECCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
NCSKLRFNQHTSWPIQAGHGCIGCSEPHFWDTMNPFEEPIGNRLYATSFNGMGADKTADT
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHH
IGAVVLGAAAIGIAAHAIISSTQNAK
HHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]